Nearshore marine construction activities often involve projects conducted directly in or adjacent to eelgrass beds and can have detrimental effects on eelgrass health, through physical destruction of beds, smothering of plants by sediment, and light reduction from turbidity. A liquefied natural gas (LNG) marine terminal is proposed to be constructed near Goldboro in Isaacs Harbour on the Eastern shore of Nova Scotia in an area where sediments are contaminated with heavy metals from historical goldmining tailings. We conducted a pre-impact assessment of the eelgrass beds in Isaacs Harbour and in adjacent contaminated and non-contaminated harbours. We used underwater video to precisely map the eelgrass bed in the direct construction footprint in Isaacs Harbour. We surveyed 169 stations along ~40 km of coastline from Wine Harbour to New Harbour to identify eelgrass presence or absence in the nearby region and provide data on the distribution and abundance of other sensitive fish habitat such as kelp and other macrophytes. Sediment samples were collected and analyzed for grain size, organic matter content and heavy metal contamination. We also collected eelgrass plants to assess plant condition using morphological and physiological metrics, and heavy metal contamination in plant tissues. The overall condition of eelgrass plants in the surveyed area fell within the range of healthy plant characteristics (morphometrics and carbohydrates reserves) seen elsewhere along the Atlantic coast. However, a few stations displayed high arsenic and mercury contamination in sediments, which translated in some cases to high contamination in eelgrass rhizomes and leaves. There would be significant risk of impact on benthic habitat and contamination of marine biota from resuspension of sediments during a construction and operation of a ship terminal in Isaacs Harbour. This pre-impact assessment will allow DFO to assess the LNG terminal construction proposal and develop appropriate mitigation and monitoring procedures. Collected data will also be used for habitat-forming species distribution modeling to inform marine spatial and conservation planning.Vercaemer, B., O’Brien, J. M., Guijarro-Sabaniel, J. and Wong, M. C. 2022. Distribution and condition of eelgrass (Zostera marina) in the historical goldmining region of Goldboro, Nova Scotia. Can. Tech. Rep. Aquat. Sci. 3513: v + 67 p.Cite this data as: Vercaemer, B., O’Brien, J. M., Guijarro-Sabaniel, J., Wong, M. Data of: Eelgrass (Zostera marina) study in the historical goldmining region of Goldboro, Nova Scotia (2020). Published: February 2023. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/ee88aa17-fd30-4d4a-8924-897fd47cf560

This collection of eelgrass data has been collated to produce a national map of the location and distribution of eelgrass beds across Canada. The data providers collaborating in this initiative include Federal, Provincial and Municipal government departments and agencies, academia, non-governmental organizations, community groups, private sector, Indigenous groups and independent science organizations. The National Eelgrass Task Force (NETForce) is a collaborative, diverse and inclusive partnership of scientists, managers, and stakeholders working towards a concrete vision which is to create a national map of eelgrass distribution in Canada that is publicly accessible, dynamic, and useful for monitoring and collective decision-making. The eelgrass data were collected using various mapping techniques including species distribution models, benthic sonar, field measurements of habitat presence or absence, video transects, aerial photography, field validation, literature review, satellite imageries, LiDAR, Airborne spectrographic imaging, and Unoccupied Aerial Vehicle (UAV). The metadata provided by the partners relevant for their own projects and the field names were made similar for the compiled dataset. We also created additional fields that differentiated the datasets, and these include data provider, institution code, water body, mapping techniques, province, biogeographic region, eelgrass observation... Other fields are included depending on the original metadata provided by the data provider (i.e. eelgrass percentage cover, eelgrass density, map reference, image classification technique). The data span from 1987 to present, with some eelgrass beds being surveyed only once while others were sampled across several years. Uncertainty information associated with a dataset is included in the metadata when available. This map is intended to be evergreen and more eelgrass data will be added when available. This compiled dataset has been collected by many organizations for different purposes, using different survey techniques and different methodologies and, therefore, considerable care must be taken when using these data. For further information concerning specific datasets contact the data provider/institution and/or see the associated technical report (if available) included in the Report folder under the ‘Data and Resources’ section.This group of eelgrass data has been divided using the geographic boundaries of the Federal Marine Bioregions (https://open.canada.ca/data/en/dataset/23eb8b56-dac8-4efc-be7c-b8fa11ba62e9). The title of each geodatabase (FGDB/GDB) contains the name of the bioregion.The Data Dictionary guide provides the fields description (English and French) from each layer included in the geodatabases.For additional information please see:Gomez C., Guijarro-Sabaniel J., Wong M. 2021. National Eelgrass Task (NET) Force: engagement in support of a dynamic map of eelgrass distribution in Canada to support monitoring, research and decision making. Can. Tech. Rep. Aquat. Sci. 3437: vi + 48 p. https://waves-vagues.dfo-mpo.gc.ca/library-bibliotheque/4098218x.pdfGuijarro-Sabaniel, J., Thomson, J. A., Vercaemer, B. and Wong, M. C. 2024. National Eelgrass Task Force (NETForce): Building a dynamic, open eelgrass map for Canada. Can. Tech. Rep. Fish. Aquat. Sci. 3583: v + 31 p. https://waves-vagues.dfo-mpo.gc.ca/library-bibliotheque/41223147.pdf

Distribution of eelgrasses in coastal British Columbia showing relative abundance (RA) and overall relative importance (RI). RI is based on project region and not on the province as a whole. CRIMS is a legacy dataset of BC coastal resource data that was acquired in a systematic and synoptic manner from 1979 and was intermittently updated throughout the years. Resource information was collected in nine study areas using a peer-reviewed provincial Resource Information Standards Committee consisting of DFO Fishery Officers, First Nations, and other subject matter experts. There are currently no plans to update this legacy data.

Distribution of Bald Eagle habitat in coastal British Columbia showing relative abundance (RA) and overall relative importance (RI). RI is based on project region and not on the province as a whole. CRIMS is a legacy dataset of BC coastal resource data that was acquired in a systematic and synoptic manner from 1979 and was intermittently updated throughout the years. Resource information was collected in nine study areas using a peer-reviewed provincial Resource Information Standards Committee consisting of DFO Fishery Officers, First Nations, and other subject matter experts. There are currently no plans to update this legacy data.

A Conservation Unit (CU) is a group of wild Pacific salmon sufficiently isolated from other groups that, if extirpated, is very unlikely to recolonize naturally within an acceptable timeframe, such as a human lifetime or a specified number of salmon generations.Holtby and Ciruna (2007) provided a framework for aggregating the five species of salmon (genus Oncorhynchus) found on Canada’s Pacific coast into species-specific CUs based on three primary characteristics: ecotypology, life history and genetics. The first stage in the description of the Conservation Units is based solely on ecology. The ecotypologies used in this framework include a combined characterization of both freshwater and near-shore marine environments, and is termed “joint adaptive zone”. The second stage of the description involves the use of life history, molecular genetics, and further ecological characterizations to group and partition the first stage units into the final Conservation Units. The result is CUs that are described through the joint application of all three axes. It is important to note that CUs are distinct from other aggregates of Pacific salmon, such as designatable units (DUs) under the Species at Risk Act or management units (MUs).CU Counting Sites:Salmon spawner enumeration data in the Pacific Region is stored and managed in the New Salmon Escapement Database (NuSEDS). The term “escapement” is used to refer to the group of mature salmon that have ‘escaped’ from various sources of exploitation, and returned to freshwater to spawn and reproduce. This data is assigned to a “Counting Site”, which may be a complete watercourse with a marine terminus, a tributary to a larger watercourse, or a defined reach within a watercourse that may or may not encompass the entire population but represents an index of the abundance of that population. CU Status:CUs form the basic unit for assessment under Canada’s Policy for the Conservation of Wild Salmon Policy (WSP) (DFO 2005). The biological status of a CU is evaluated using a number of metrics (Holt et al. 2009; Holt 2009), which indicate a WSP status zone: Red (poor status), Amber (marginal status), or Green (healthy status). A final step then incorporates all metric and status-related information into a final integrated status for each CU, along with expert commentary to support the final status determination (e.g., DFO 2012; DFO 2016). This information is used as inputs to fisheries management processes to help prioritize assessment activities and management actions.Note: CU boundaries were reviewed in 2020-2021 and have been updated from the BC Freshwater Atlas 1:50,000 scale to the BC Freshwater Atlas 1:20,000 scale. The CU boundaries were last updated in March 2023. Please be aware that CUs may be reviewed and are subject to change without notice.Please refer to Conservation Unit Review Requests-Form and Summary for a list of CU review requests that are ongoing or have been finalized.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

A Conservation Unit (CU) is a group of wild Pacific salmon sufficiently isolated from other groups that, if extirpated, is very unlikely to recolonize naturally within an acceptable timeframe, such as a human lifetime or a specified number of salmon generations.Holtby and Ciruna (2007) provided a framework for aggregating the five species of salmon (genus Oncorhynchus) found on Canada’s Pacific coast into species-specific CUs based on three primary characteristics: ecotypology, life history and genetics. The first stage in the description of the Conservation Units is based solely on ecology. The ecotypologies used in this framework include a combined characterization of both freshwater and near-shore marine environments, and is termed “joint adaptive zone”. The second stage of the description involves the use of life history, molecular genetics, and further ecological characterizations to group and partition the first stage units into the final Conservation Units. The result is CUs that are described through the joint application of all three axes. It is important to note that CUs are distinct from other aggregates of Pacific salmon, such as designatable units (DUs) under the Species at Risk Act or management units (MUs).CU Counting Sites:Salmon spawner enumeration data in the Pacific Region is stored and managed in the New Salmon Escapement Database (NuSEDS). The term “escapement” is used to refer to the group of mature salmon that have ‘escaped’ from various sources of exploitation, and returned to freshwater to spawn and reproduce. This data is assigned to a “Counting Site”, which may be a complete watercourse with a marine terminus, a tributary to a larger watercourse, or a defined reach within a watercourse that may or may not encompass the entire population but represents an index of the abundance of that population. CU Status:CUs form the basic unit for assessment under Canada’s Policy for the Conservation of Wild Salmon Policy (WSP) (DFO 2005). The biological status of a CU is evaluated using a number of metrics (Holt et al. 2009; Holt 2009), which indicate a WSP status zone: Red (poor status), Amber (marginal status), or Green (healthy status). A final step then incorporates all metric and status-related information into a final integrated status for each CU, along with expert commentary to support the final status determination (e.g., DFO 2012; DFO 2016). This information is used as inputs to fisheries management processes to help prioritize assessment activities and management actions.Note: CU boundaries were reviewed in 2020-2021 and have been updated from the BC Freshwater Atlas 1:50,000 scale to the BC Freshwater Atlas 1:20,000 scale. The CU boundaries were last updated in March 2023. Please be aware that CUs may be reviewed and are subject to change without notice.Please refer to Conservation Unit Review Requests-Form and Summary for a list of CU review requests that are ongoing or have been finalized.

A compendium of reports that provide information about aquatic and terrestrial animals and plants, soils, surface water, groundwater and their accompanying data files and maps

DescriptionConservation of marine biodiversity requires understanding the joint influence of ongoing environmental change and fishing pressure. Addressing this challenge requires robust biodiversity monitoring and analyses that jointly account for potential drivers of change. Here, we ask how demersal fish biodiversity in Canadian Pacific waters has changed since 2003 and assess the degree to which these changes can be explained by environmental change and commercial fishing. Using a spatiotemporal multispecies model based on fisheries independent data, we find that species density (number of species per area) and community biomass have increased during this period. Environmental changes during this period were associated with temporal fluctuations in the biomass of species and the community as a whole. However, environmental changes were less associated with changes in species’ occurrence. Thus, the estimated increases in species density are not likely to be due to environmental change. Instead, our results are consistent with an ongoing recovery of the demersal fish community from a reduction in commercial fishing intensity from historical levels. These findings provide key insight into the drivers of biodiversity change that can inform ecosystem-based management.The layers provided represent three community metrics: 1) species density (i.e., species richness), 2) Hill-Shannon diversity, and 3) community biomass. All layers are provided at a 3 km resolution across the study domain for the period of 2003 to 2019. For each metric, we provide layers for three summary statistics: 1) the mean value in each grid cell over the temporal range, 2) the probability that the grid cell is a hotspot for that metric, and 3) the temporal coefficient of variation (i.e., standard deviation/mean) across all years.Methods:The analysis that produced these layers is presented in Thompson et al. (2022). The analysis uses data from the Groundfish Synoptic Bottom Trawl Research surveys in Queen Charlotte Sound (QCS), Hecate Strait (HS), West Coast Vancouver Island (WCVI), and West Coast Haida Gwaii (WCHG) from 2003 to 2019. Cartilaginous and bony fish species caught in DFO groundfish surveys that were present in at least 15% of all trawls over the depth range in which they were caught were included. This depth range was defined as that which included 95% of all trawls in which that species was present. The final dataset used in our analysis consisted of 57 species (Table S1 in Thompson et al. 2022).The spatiotemporal dynamics of the demersal fish community were modeled using the Hierarchical Modeling of Species Communities (HMSC) framework and package (Tikhonov et al. 2021) in R. This framework uses Bayesian inference to fit a multivariate hierarchical generalized mixed model. We modeled community dynamics using a hurdle model, which consists of two sub models: a presence-absence model and a biomass model that is conditional on presence. Our list of environmental covariates included bottom depth, bathymetric position index (BPI), mean summer tidal speed, substrate muddiness, substrate rockiness, whether the trawl was inside or outside of the ecosystem-based trawling footprint, and survey region (QCS & HS vs. WCVI & WCHG)), mean summer near-bottom temperature deviation, mean summer near-bottom dissolved oxygen deviation, mean summer cross-shore and along-shore current velocities near the seafloor, mean summer depth-integrated primary production, and local-scale commercial fishing effort.Layers are provided for three community metrics. All metrics should be interpreted as the value that would be expected in the catch from an average tow in the Groundfish Synoptic Bottom Trawl Research Surveys taken in a given 3 km grid cell. Species density (sometimes called species richness) should be interpreted as the number of the 57 species that would be caught in a trawl. Hill-Shannon diversity is a measure of diversity that gives greater weight to communities where biomass is spread equally across species. Community biomass is the total biomass across all 57 species that would be expected to be caught per square km in an average tow. Data Sources:Research data was provided by Pacific Science's Groundfish Data Unit for research surveys from the GFBio database between 2003 and 2019 that occurred in four regions: Queen Charlotte Sound, Hecate Strait, West Coast Haida Gwaii, and West Coast Vancouver Island. Our analysis excludes species that are rarely caught in the research trawls and so our estimates would not include the occurrence or biomass of these rare species.Commercial fishing data was accessed through a DFO R script detailed here: https://github.com/pbsassess/gfdata. Local scale commercial fishing effort was calculated from this data. The substrate layers were obtained from a substrate model (Gregr et al. 2021). The oceanographic layers (bottom temperature, dissolved oxygen, tidal and circulation speeds, primary production) were obtained from a hindcast simulation of the British Columbia continental margin (BCCM) model (Peña et al. 2019).Uncertainties:Species that are not well sampled by the trawl surveys may not be accurately estimated by our model. The model did not include spatiotemporal random effects, which likely underestimates spatiotemporal variability in the region. It is also important to underline covariate uncertainty and model uncertainty. The hotspot estimates provide one measure of model uncertainty/certainty.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.