In 2018, Fisheries and Oceans Canada initiated the Multidisciplinary Arctic Program (MAP) – Last Ice, the first ecosystem study of the poorly characterized region of the Lincoln Sea in the Marine Protected Area of Tuvaijuittuq, where multiyear ice still resides in the Arctic Ocean. MAP-Last Ice takes a coordinated approach to integrate the physical, biochemical, and ecological components of the sea ice-ocean connected ecosystem and its response to climate and ocean forcings. The cross-disciplinary program establishes baseline ecological knowledge for Tuvaijuittuq and, in particular, for its unique multiyear ice ecosystem. The database provides baseline data on the abundance of bacteria and viruses in multi- and first-year ice and in surface waters of the Lincoln Sea in Tuvaijuittuq, and their relation to bio-physical conditions. The data were collected during the 2018 spring field campaign of the MAP-Last Ice Program, at an ice camp offshore of Canadian Forces Station (CFS) Alert.

This record contains information on the samples collected and the intended parameters for analysis from sediment cores collected as part of this project including bacteria, chemistry, stable isotopes, fatty acids, or meiofauna.

Bay-scale empirical demonstrations of how bivalve aquaculture alters plankton composition, and subsequently ecological functioning and higher trophic levels, are lacking. Temporal, inter- and within-bay variation in hydrodynamic, environmental, and aquaculture pressure limit efficient plankton monitoring design to detect bay-scale changes and inform aquaculture ecosystem interactions. Here, we used flow cytometry to investigate spatio-temporal variations in bacteria and phytoplankton (< 20 µm) composition in four bivalve aquaculture embayments. We observed higher abundances of bacteria and phytoplankton in shallow embayments that experienced greater freshwater and nutrient inputs. Depleted nutrient conditions may have led to the dominance of picophytoplankton cells, which showed strong within-bay variation as a function of riverine vs freshwater influence and nutrient availability. Although environmental forcings appeared to be a strong driver of spatio-temporal trends, results showed that bivalve aquaculture may reduce near-lease phytoplankton abundance and favor bacterial growth. We discuss aquaculture pathways of effects such as grazing, benthic-pelagic coupling processes, and microbial biogeochemical cycling. Conclusions provide guidance on optimal sampling considerations using flow cytometry in aquaculture sites based on embayment geomorphology and hydrodynamics.Cite this data as: Sharpe H, Lacoursière-Roussel A, Barrell J (2024). Monitoring bay-scale bivalve aquaculture ecosystem interactions using flow cytometry. Version 1.2. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=monitoring_bay-scale_bivalve_aquaculture_ecosystem_interactions_using_flow_cytometry&v=1.2

Cyanobacteria (also referred to as blue-green algae) are common photosynthetic bacteria that live in surface waters. Under favorable conditions, such as warm water and high nutrient content, these bacteria can form nuisance “blooms”. The presence of blooms in recreational water causes unpleasant aesthetics and exposure to some toxin-producing blooms may pose potential health risks. Contact with blooms can cause skin rashes and irritation, itchy eyes, and ear infections. Inhaling water may cause allergic-like reactions, runny noses or sore throats. Ingestion of toxins can cause a range of symptoms (e.g., hepatotoxic or neurotoxic effects, and even death). There has been increased public awareness as a result of research over the past 25 years, recent monitoring efforts, and increased public education on the topic. In 2009, Alberta Health and Alberta Health Services began seasonal monitoring for cyanobacterial blooms at high use recreational beaches. In 2019, beach operators took over the sampling role from Alberta Health Services under the Alberta Safe Beach Protocol. Water is collected from shallow water adjacent to beaches and submitted to laboratories for analysis of cyanobacterial bloom indicators. These data, along with visual inspection, are used to characterize potential cyanobacterial blooms and issue recreational water use advisories when cyanobacteria are found in a waterbody at levels that can affect human health. The data presented below is organized into two files that contain supporting data and key cyanobacterial bloom indicators, and counts of individual cyanobacteria species, respectively. Each row represents a water sample collected from an Alberta beach. In the cyanobacteria species data, each water sample will have many associated rows of data. Each column represents a piece of information about that water sample (e.g., key indicators and supporting information) that is used to characterize cyanobacterial blooms. Data from the current year (2025) should be considered preliminary and might change with further quality control/quality assurance steps. This dataset is updated monthly between June and September each year. For more information on these indicators please refer to the column descriptions “Usage Considerations” associated with this dataset.

PURPOSE:The purpose of this work was to determine (1) how the population genomic structure relates to gut microbiota composition of White Hake (Urophycis tenuis), and (2) whether microbiota community variation provides complementary insights into population structure of this species in eastern Canada.DESCRIPTION:Integrating host-associated microbiota with genomic approaches offers an opportunity to better understand the multiple biological dimensions shaping population structure in marine fishes. A clear understanding of population structure and dynamics is essential for informed fisheries management and conservation decisions; however, while genomic approaches have greatly improved our ability to delineate biological populations, they provide only a partial representation of biological structure, as patterns of differentiation reflect both historical divergence and contemporary ecological conditions. Host-associated microbiota can influence population-level ecological processes by contributing functional, potentially heritable variation that shapes host phenotype and fitness.In this study, we combined genotyping-by-sequencing with 16S rRNA gene amplicon sequencing to examine how population genomic structure relates to gut microbiota composition and to evaluate whether microbiota community variation provides complementary insights into population structure in White Hake (Urophycis tenuis) in eastern Canada. Genomic analyses identified two populations with greater spatial overlap than previously reported. Variation partitioning revealed that host genetics explained a negligible proportion of microbiota variation compared to environmental factors and fish length, suggesting that ontogenetic shifts in habitat use and resource acquisition influence gut microbiota composition. Several taxa were differentially abundant among fish length categories used as a proxy for diet, including taxa with chitin-degrading potential such as Photobacterium and Lachnospirales, which were enriched in smaller fish known to consume a crustacean-dominated diet. Together, these results indicate that gut microbiota composition in White Hake primarily reflects ecological and life-history processes rather than host population structure. PARAMETERS COLLECTED:Environmental parameters were also collected at most sampling sites, including depth, water temperature, oxygen and salinity levels.PHYSICAL SAMPLE DETAILS:Fin samples were collected to characterize the fish genotypes. Intestine (rectum) samples were collected to study fish gut microbiome.SAMPLING METHODS:In 2022 and 2023, White Hake were sampled during Fisheries and Oceans Canada (DFO) annual bottom trawl ecosystem monitoring surveys.USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Historical finds of Coleophora serratella

Historical finds of Fenusa pumila

This new data class brings over data from the Waste Management Information System (WMIS), which is a Microsoft Access based database used by the Ministry of Natural Resources (MNR) to track Waste Management Sites. This was married with the spatial data from Waste Disposal Sites where possible Different Waste Disposal Site types collected by the Ministry of Natural Resources include: * Compost Disposal * Hazardous Waste Disposal * Household Waste Disposal * Industrial Waste Disposal * Septic Drying Bed * Septic Field * Sewage Disposal * Tile Bed * Transfer Station This product requires the use of geographic information system (GIS) software.

Historical finds of Coleophora laricella

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b