AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas.Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador.Reference:TunicatesViolet tunicate (Botrylloides violaceus) 2007The violet tunicate was first detected in NL waters in 2007 in Belleoram, Fortune Bay on wharf structures and vessels (McKenzie et al. 2016b). This colonial tunicate forms irregular shaped colonies usually of a solid color (orange, purple, yellow or cream). It is currently found in relatively small colonies in four harbours in NL; Placentia Bay (1), Fortune Bay (1), Conception Bay (1) and the west coast of NL (2). The data provided here indicates the detections of this AIS in coastal NL.From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

Historical finds of Coleophora laricella

Historical finds of Coleophora serratella

A research survey of scallops (mainly sea scallop Placopecten magellanicus, but also Icelandic Scallop Chlamys islandica) using a dredge was carried out by DFO (Fisheries and Oceans Canada) every 1 or 2 years since 1992 in the Magdalen Islands (fishing area 20). The main objective of this research survey was to assess Sea Scallop stocks. Another objective was to document taxa associated with scallop habitat according to a fixed random sampling plan. Occurrences for the year 2021 and 2022 are presented by species (or taxon) by station. Starting in 2021, catches were weighed, and specimens photographed, with information available upon request. The taxonomic and geographical validity of the data was checked and the World Register of Marine Species served as the taxonomic authority for naming all taxa recorded during the survey. Epibenthic invertebrates (mainly molluscs, echinoderms and crustaceans) as well as demersal fish were identified from the dredge catches. The historical dataset is available at the following link : https://open.canada.ca/data/en/dataset/71732ad5-5c70-4dbf-916d-a94e1380c53bThe study area is located south of the Magdalen Islands and the sampling of scallop beds is carried out at depths of generally around 25 to 35 m. A random selection of sampling stations is carried out from a fixed station grid. Sampling is done along transects at these randomly drawn stations in the study area. Sampling is done with a lined Digby scallop dredge (20 mm mesh) over approximately 500 m along the seabed. The four baskets of the dredge are examined for all scallops, and starting in 2022, also for all fishes. One basket (first on the starboard side) is sorted and examined for associated species. Most specimens are counted by taxa. Those that are too small and numerous, or colonial, are noted for presence or relative abundance. Special cases are occasionally conserved for taxonomic analysis, for example, ascidians (to monitor for invasive species) and sponges (to document new species). The availability of photos and some conserved specimens enables future review. Changes are anticipated in the identifications, notably for Bryozoan, Hydrozoa, and Porifera, that are currently the focus of research efforts.

Historical finds of Gilpinia hercyniae

Historical finds of Adelges abietis

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

Historical finds of Pristiphora erichsonii

Historical finds of Pristiphora geniculata

Historical finds of Fenusa pumila