An exploratory project on the taxonomic and genetic diversity of decapods in three ocean subregions (Northeast Pacific, Canadian Arctic, and Northwest Atlantic), which were sampled in 2022, was undertaken by the Arctic Working Group under the Canada-U.S. Fisheries and Climate Collaboration between Fisheries and Oceans Canada (DFO) and the National Marine Fisheries Service (NMFS) of the National Oceanic and Atmospheric Administration (NOAA). This collaboration framework aims to pool Canadian and U.S. data to explore the impacts of broad-scale climate change on marine biodiversity. In early summer 2022, a sampling protocol with the selection of targeted decapods was provided to DFO and NOAA collaborators. Targeted genera were collected from a total of 10 research programs across three ocean subregions and four marine regions. The Northeast Pacific samples were collected in the Bering Sea during the Northern Bering Sea Ecosystem and Surface Trawl Survey, and the Eastern and Northern Bering Sea Continental Shelf Bottom Trawl Survey of Groundfish and Invertebrate Fauna onboard the F/V Northwest Explorer, F/V Alaska Knight and F/V Vesteraalen. In the Western Canadian Arctic (mainly from Beaufort Sea and Amundsen Gulf), specimens were collected during DFO’s Canadian Beaufort Sea – Marine Ecosystem Assessment (CBS-MEA) survey onboard the F/V Frosti. In Eastern Canadian Arctic (mainly from Baffin Bay and Davis Strait), specimens were collected during DFO’s Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) survey onboard the CCGS Amundsen and DFO’s North Atlantic Fisheries Organization (NAFO) Subarea 0B survey onboard the R/V Tarajoq. In the Estuary and Gulf of St. Lawrence (EGSL), specimens were collected from coastal surveys (scallops, sea cucumber, snow crab, and whelk surveys) onboard the CCGS Leim and offshore during the Ecosystemic Survey onboard the CCGS Teleost. Decapods were collected from various sampling gears (benthic beam trawl, modified Atlantic Western IIA otter trawl, Bacalao trawl, shrimp trawl, Digby scallop dredge, or modified sea cucumber dredge) and identified to the lowest possible taxonomic level and photographed, when possible. All specimens were frozen at sea (n = 995). In the lab, the identifications were validated or refined with the photos and the frozen specimens. DNA was extracted for 87 specimens and a section of COI gene was amplified in order to be sequenced using Sanger method. Sequences were compared with existing data using The Basic Local Alignment Search Tool (BLAST) in the National Center for Bio-technology Information Nucleotide database (NCBI-nt, including the GenBank database) to compare scientific names, where available.The present dataset includes 391 decapod species occurrences. DNA was extracted for a subset of 87 specimens (COI gene); sequences are publicly available on BOLD data portal under project code DDAO (see supporting document "citations_references.csv" for more information).The data are presented in Darwin Core format and are separated in three files:The "Activité_décapodes_DDAO_decapods_event_en" file contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The "Occurrence_décapodes_DDAO_decapods_en" file contains the taxonomic occurrences.The "ADN_décapodes_DDAO_decapods_DNA_en" file contains the DNA derived data.For further details, please refer to the technical report available in the supporting document named "citations_references.csv". USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Historical finds of Profenusa thomsoni

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

This dataset contains the data reported in Wesley R Ogloff, Randi A Anderson, David J Yurkowski, Cassandra D Debets, W Gary Anderson, Steven H Ferguson, Spatiotemporal variation of ringed seal blubber cortisol levels in the Canadian Arctic, Journal of Mammalogy, 2022;, gyac047, https://doi.org/10.1093/jmammal/gyac047Cite this data as:Wesley R Ogloff, Randi A Anderson, David J Yurkowski, Cassandra D Debets, W Gary Anderson, Steven H Ferguson. 2022 Spatiotemporal variation of ringed seal blubber cortisol levels in the Canadian Arctic. Arctic and Aquatic Research Division, Fisheries and Oceans Canada, Winnipeg, MB. https://open.canada.ca/data/en/dataset/e1c6b350-0159-11ed-8212-1860247f53e3

PURPOSE:To characterize food webs of lake fish communities using stable isotopes, gut content morphology and DNA.DESCRIPTION:Data sets containing fish species caught in Miramichi Lake, McKiel Lake and Nashwaak Lake. PARAMETERS COLLECTED:Species counts (ecological); points (spatial)USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Historical finds of Coleophora laricella

Since 1985, research surveys targeting scallops—primarily the sea scallop (Placopecten magellanicus) and, to a lesser extent, the Icelandic scallop (Chlamys islandica)—have been conducted by Fisheries and Oceans Canada (DFO) at one- or two-year intervals around the Magdalen Islands (fishing area 20A). The main objective of this survey is to assess the status of sea scallop stocks. The study area is situated south of the Magdalen Islands, where scallop beds are typically sampled at depths ranging from approximately 25 to 35 m. Sampling stations are randomly selected from a predetermined fixed grid, with sampling conducted along transects at these randomly assigned locations within the study area. Each station is sampled using a lined Digby scallop dredge (20 mm mesh size), towed for roughly 500 m across the seabed.This publication includes three files: the file biometriePetoncle_20, which contains detailed biometric data (species, size, weights and sex) from 1998 to 2024; the file taillePetoncle_20, which provides the size of the individuals sampled from 2009 to 2024; and the file traitPetoncle_20 which contains the abundances and densities per tow from 2009 to 2024. Data on abundances and densities per tow from 1998-2008 is available upon request.This dataset is updated every one to two years as data becomes available. A cleaning of aberrant data has been carried out. However, there is missing data in various columns of the dataset – use the data with caution. If you have any questions please contact DFO.DataManagementSAISB-GestionDonneesDAISS.MPO@dfo-mpo.gc.ca or the author. For certain time periods, associated species are identified and semi-quantitatively counted directly on the sorting table, and the results are presented in the following publications: - https://open.canada.ca/data/en/dataset/6529a4b0-f863-4568-ac71-1fa26cf68679- https://open.canada.ca/data/en/dataset/71732ad5-5c70-4dbf-916d-a94e1380c53b 

A research survey of scallops (mainly sea scallop Placopecten magellanicus, but also Icelandic Scallop Chlamys islandica) using a dredge was carried out by DFO (Fisheries and Oceans Canada) every 1 or 2 years since 1992 in the Magdalen Islands (fishing area 20). The main objective of this research survey was to assess Sea Scallop stocks. Another objective was to document taxa associated with scallop habitat according to a fixed random sampling plan. Occurrences by species (or taxon) are presented by station. The taxonomic and geographical validity of the data was checked and the World Register of Marine Species served as the taxonomic authority for naming all taxa recorded during the survey. Epibenthic invertebrates (mainly molluscs, echinoderms and crustaceans) as well as demersal fish were identified from the dredge catches. The current data starting in 2021 are available at the following link : https://open.canada.ca/data/en/dataset/6529a4b0-f863-4568-ac71-1fa26cf68679The study area is located south of the Magdalen Islands and the sampling of scallop beds is carried out at depths of 10 to 38 m, generally around 25 to 35 m. A random selection of sampling stations is carried out from a fixed station grid. Sampling is done along transects at these randomly drawn stations in the study area. Sampling is done with a lined Digby scallop dredge (20 mm mesh) over approximately 500 m along the seabed. The four baskets of the dredge are examined for all scallops. Next, a basket (the first on the starboard side) is sorted and examined for associated species. Most specimens are counted by taxon. The presence or relative abundance of undersized and numerous, or colonial, organisms is noted. Special cases are sometimes retained for taxonomic analysis, for example, ascidians (to monitor invasive species) and sponges (to document new species).

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