To support the surveillance of key macroalgae and non-indigenous species in Nova Scotia and New Brunswick, five quantitative PCR (qPCR) assays were designed and tested at 111 sites in 2022-2023 targeting the following non-indigenous macroalgal species: Antithamnion sparsum, Bonnemaisonia hamifera, Codium fragile, Dasysiphonia japonica, Fucus serratus. All assays were developed in 2022 by the Center for Environmental Genomics Applications (CEGA, Newfoundland, Canada) except Antithamnion sparsum, for which an assay was developed in 2023 by the Aquatic Biotechnology Laboratory (ABL) at the Bedford Institute of Oceanography. All amplification was performed by the ABL in 2022-2023. The assay developed for Fucus serratus was later determined to be non-specific, and amplifies both F. serratus and Fucus distichus.Cite this data as: Krumhansl K, Brooks C, Lowen B, DiBacco C, (2025). Quantitative PCR (qPCR) of Key Macroalgal Non-Indigenous Species in Nova Scotia and New Brunswick Waters. Version 1.5. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=quantitative_qpcr_macroalgal_nonindigenous_species_novascotia_newbrunswick_2022_2023&v=1.5For additional information please see:LeBlanc F., Belliveau V., Watson E., Coomber C., Simard N., DiBacco C., Bernier R., Gagné N. 2020. Environment DNA (eDNA) detection of marine aquatic invasive species (AIS) in Eastern Canada using a targeted species-specific qPCR approach. Management of Biological Invasions 11(2):201-217Krumhansl K.A., Brooks C.M., Lowen B., O’Brien J., Wong M., DiBacco C. Loss, resilience and recovery of kelp forests in a region of rapid ocean warming. Annals of Botany 2024 Mar 8; 133(1):73-92Brooks C.M., Krumhansl K.A. 2023. First record of the Asian Antithamnion sparsum Tokida, 1932 (Ceramiales, Rhodophyta) in Nova Scotia, Canada. BioInvasions Records 12(3):745-725.

PURPOSE:Provide early detection of newly arrived Aquatic Invasive Species (AIS) and determine the spread, establishment and spatial distribution of existing AIS within marine waters of the southern Gulf of St. Lawrence (sGSL), DFO Gulf Region boundaries (northern and eastern coastal shores of NB, Gulf shore of NS, and PEI shoreline).DESCRIPTION:DFO Science monitors for AIS in the Gulf Region. The data collected from DFO's biofouling monitoring program provides an overview of the distribution and abundance of Aquatic Invasive Species (AIS) in the Gulf Region. This information can be used by the general public, scientists and DFO managers.Monitoring program targeting aquatic invasive species (AIS). Native biofouling species are not included in this dataset. Botrylloides violaceus: Violet tunicateBotryllus schlosseri: Golden star tunicateCiona intestinalis: Vase tunicateStyela clava: Clubbed tunicateCaprella mutica*: Japanese skeleton shrimpMembranipora membranacea: Coffin box bryozoanCarcinus maenas*: European green crabCodium fragile*: Oyster thief algaJuxtacribrilina mutabilis**: Ribbed bryozoan (JCM)*indicates species that are not included as percent cover as they are not accurately captured by the sampling method, but are included as detections.**indicates species was only integrated fully into the monitoring protocol starting in 2025.Included here is a dataset of detection and percent cover data of AIS, as well as a monitoring station dataset. Environmental data collected, including from temperature loggers, are stored but not included here. PARAMETERS COLLECTED:Air and water temperature, salinity, depth, dissolved oxygen, weather conditions, list of biofouling AIS, percent cover of AIS on PVC plates, and incidental detection of other species and AIS in surrounding area. Only species data is pushed to the Open Data platform.NOTES ON QUALITY CONTROL:Each sample and species is processed and identified in a standardized fashion using standardized DFO Science AIS protocols and taxonomic references. Data is manually entered into DFO Gulf Region's AIS Science biofouling database and randomly verified for accuracy.SAMPLING METHODS:Biofouling monitoring is conducted using PVC collector plates that are deployed in the water column approximately 1 meter below the sea surface in the spring of each year. Biofouling organisms settle on these plates which are collected in the fall of the same year. Abundances of AIS are given as percent plate cover. Physico-chemical data including temperature, conductivity, and depth as well as weather conditions are noted at each geo-referenced biofouling monitoring site during initial deployment and at time of retrieval. All biofouling organisms settled on the underside of the PVC plates are noted and percent cover of each AIS is estimated. Any AIS present at the location is also noted. A HOBO temperature and light logger was deployed.USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Sensitive Ecosystems Inventory (SEI) project boundaries (study areas) contains attributes describing each project (project level metadata), and includes links to the locations of other data associated with the project (e.g. reports, polygon datasets, plot files). SEI identifies and maps rare and fragile terrestrial ecosystems in a given area for the purpose of encouraging land-use decisions that will ensure the continued integrity of these ecosystems. This layer is derived from the STE_TEI_PROJECT_BOUNDARIES_SP layer by filtering on the PROJECT_TYPE attribute. Project types include: SEI, TEMSEI, TEMSET, TEMSEW and SEIWHR. Current version: v11 (published on 2024-10-03) Previous versions: v10 (published on 2023-11-14), v9 (published on 2023-03-01), v8 (published on 2016-09-01)

SEI_Polygons contains Sensitive Ecosystems Inventory polygons with key and amalgamated (concatenated) attributes derived from the RISC (Resource Inventory Standards Committee) standard attributes. SEI identifies and maps rare and fragile terrestrial ecosystems. Ecosystems mapped may include (but are not limited to) older forests, woodlands, coastal bluffs, herbaceous and sparsely vegetated ecosystems, grasslands, riparian ecosystems and wetlands. SEI methods include manual air photo interpretation or theming of other Ecosystem Mapping, each supported by selective field checking. This layer is derived from the STE_TEI_ATTRIBUTE_POLYS_SP layer by filtering on the PROJECT_TYPE attribute. Project types include: SEI, TEMSEI, TEMSET, and SEIWHR. Current version: v11 (published on 2024-10-03) Previous versions: v10 (published on 2023-11-14), v9 (published on 2023-03-01), v8 (published on 2016-09-01)

The Coastal Oceanography and Ecosystem Research section (DFO Science) reviewed the presence of Cod in the Population Ecology Division (DFO Science) Ecosystem Survey trawls to describe the likelihood of presence. The survey consists of a stratified random design using a bottom trawl. This layer was created for consideration in oil spill response planning.A version of this dataset was created for the National Environmental Emergency Center (NEEC) following their data model and is available for download in the Resources section.Cite this data as: Lazin, G., Hamer, A.,Corrigan, S., Bower, B., and Harvey, C. Data of: Likelihood of presence of Atlantic Cod in Area Response Planning pilot areas. Published: June 2018. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, St. Andrews, N.B. https://open.canada.ca/data/en/dataset/af2bf6c0-481d-4445-bbc6-7a785d2a9aa9

This project was completed by the Salmonids Section in the Newfoundland and Labrador Science Branch of Fisheries and Oceans Canada (DFO). The Coastal Environmental Baseline Program has supported efforts in 2018 and 2019 of tagged Atlantic salmon smolts leaving the Bay de L’eau River and Rushoon River region of Placentia Bay. This was part of a larger four year tracking study in this region (2018, 2019, 2021, 2022) trying to improve DFO’s understanding of the residency, survival, and migration routes of Atlantic salmon smolts during the first months at sea within northwest Placentia Bay. As of spring 2023, four years of detection data were being processed with the goal of presenting this work at the next Atlantic salmon CSAS meeting and developing a primary publication. This record contains the locations there smolt were tagged in Placentia Bay, NL.

A magnitude 5 earthquake scenario along an unnamed fault located about 15 km north-northeast of Burnaby City Hall and directly south of Mt Elsay. This fault is not known to be active, but this scenario represents a small but damaging event in the North Shore Mountains.

Monitoring of Unit 2 redfish by Fisheries and Oceans Canada (DFO) ceased in 2002. Since then, the Atlantic Groundfish Council (AGC, formerly the Groundfish Enterprise Allocation Council [GEAC]) has funded surveys approximately every two years in the area, in collaboration with DFO. Over the years, various vessels and gear types have been used. In 2024, a comparative survey was conducted using the CCGS John Cabot and a fishing industry vessel (the Léry Charles) to develop conversion factors that allow data from the 2020, 2022, and 2024 Unit 2 redfish surveys to be standardized to CCGS John Cabot equivalents. The survey covered the waters off southwestern Newfoundland and eastern Cape Breton, corresponding to redfish management Unit 2, which includes NAFO subdivisions 3Pn, 3Ps, 4Vn, 4Vs, and 4W. Here, data collected aboard the CCGS John Cabot are presented. Objectives• Assess the abundance and condition of groundfish and invertebrates• Assess environmental conditions• Inventory the biodiversity of benthic and demersal megafauna• Monitor the pelagic ecosystem• Collect samples for various research projectsData The CCGS John Cabot employed a four-sided modified Campelen 1800 shrimp trawl, fitted with a Rockhopper ("bicycle") footgear. The trawl extension and codend were lined with 12.7 mm knotless nylon mesh. Standard trawl tows were 15 minutes in duration, timed from bottom contact, with a target towing speed of 3 knots.For each fishing tow, the catch is sorted and weighed by taxa; individuals are then counted and biological data are collected on a subsample. For fish, crab and squid, size and weight are measured by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish, and the otoliths are sampled for several species such as Atlantic cod, Atlantic halibut, and Greenland halibut. Roughly 2-kg of shrimp are sorted and weighed by species. The other invertebrates are counted (no individual measurements) and photographed.The biological data are divided into 3 files: a “Stations” file containing set information, a “Catches” file containing catches per set for all species caught and a “Carbio” file containing biological and morphometric measurements per individual all species measured except shrimps. The columns source_info, no_survey, nbpc and station_number serve as a common key linking the three datasets. To further link the “Captures” and the “Carbio” files to the species level (for fish and certain invertebrate species), use the columns source_info, no_survey, nbpc, station_number, strap_code, and category. Further shrimp biological and morphometric data are available but are not presented here in order to simplify data presentation. For access to the complete information, please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca). The latest published data is preliminary and will be updated at the end of January of the following year.It is important to note that this is raw data. Only sets considered successful are retained. In each set, all species are kept, with a few exceptions. Data is available from 1997-2022 but please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca) for access and further details. For any other information please also contact the data management team.

The Department of Fisheries established a survey of demersal juvenile Atlantic cod (*Gadus morhua*) in the nearshore (<10 m deep) in 1959. This survey aimed to characterize the distribution and abundance of juvenile Atlantic cod and was based upon Norway's Flodevigen sampling program which has been conducted continuously since 1919. A 25 m seine was used to sample juvenile Atlantic cod nursery locations on the Avalon Peninsula and Northeast coast of the island of Newfoundland in September and October. The survey continued until 1964 and became known as the Fleming survey, after original initiator Alistair Fleming. The survey was reinstated by Memorial University of Newfoundland from 1992 to 1997. Multiple tows were conducted at a subset of the original 55 Fleming sites located in St. Mary's Bay, Trepassey Bay, the Southern Shore, Conception Bay, Trinity Bay, Bonavista Bay, Gander Bay, New World Island, Fortune Harbour, Badger Bay, Halls Bay and Green Bay.This data set includes several different subsets, some of which span both Fleming Survey periods (1959-1964 and 1992-1997):I. JuvCodCatch60s90s:Catches of juvenile Atlantic cod (1959-1964, 1992-1997) from the first two consecutive tows at each site. This is a summary based on JuvCodLengths90s and FlemingSurveyData60s;II. JuvCodLength90s:Lengths of all individual juvenile Atlantic cod caught for each site (1992-1997);III. SiteEnvData60s90s:Station data for Fleming data 1992-1997;IV. FlemingSurveyData60s:Fleming survey data from 1959-1964 (note there are three record types pertaining to: station data "type 1"; general species catch data including juvenile Atlantic cod grouped by age class ("1+", "Zeroes", and "Total") "type 2; and juvenile Atlantic cod (species 223) listed in length bins "type 3"; andV. FlemingBycatch60s92to96:Bycatch data from 1959-1964 & 1992-1996 from first two tows at each site. This is a summary based partly on the FlemingSurveyData60s set.

Location and contact information for Pacific Recreational Fishery Salmon Head Recovery Depots.The sport fishing community has an important role in the recovery of coded-wire tags found in Coho and Chinook. A coded-wire tag is a 1mm piece of wire that is laser etched with a unique number. Tags are injected into the nose cartilage of juvenile salmon prior to ocean migration. Annually, Canada and the United States tag over 50 million juvenile salmon. Fisheries and Oceans Canada applies about 5.5 million tags, using about 5.5 kilometres of wire. Anglers can recognize the presence of a coded-wire tag because of the missing adipose fin (located on the dorsal surface of the salmon). If you have caught an adipose fin clipped Coho and Chinook, it is a simple matter of removing the head from the fish, completing a sport head label and then submitting the head to a Sport Head Recovery Depot in the area. It is just as important to turn in heads from terminal or freshwater sites as it is from marine areas. Even though anglers fishing close to hatcheries can be fairly certain of the origin of their catch, data will not be recorded unless the heads from fin-clipped recoveries are turned in. Without the data, the health of the stock and the value of the resource to anglers could be underestimated.