To support the surveillance of key macroalgae and non-indigenous species in Nova Scotia and New Brunswick, five quantitative PCR (qPCR) assays were designed and tested at 111 sites in 2022-2023 targeting the following non-indigenous macroalgal species: Antithamnion sparsum, Bonnemaisonia hamifera, Codium fragile, Dasysiphonia japonica, Fucus serratus. All assays were developed in 2022 by the Center for Environmental Genomics Applications (CEGA, Newfoundland, Canada) except Antithamnion sparsum, for which an assay was developed in 2023 by the Aquatic Biotechnology Laboratory (ABL) at the Bedford Institute of Oceanography. All amplification was performed by the ABL in 2022-2023. The assay developed for Fucus serratus was later determined to be non-specific, and amplifies both F. serratus and Fucus distichus.Cite this data as: Krumhansl K, Brooks C, Lowen B, DiBacco C, (2025). Quantitative PCR (qPCR) of Key Macroalgal Non-Indigenous Species in Nova Scotia and New Brunswick Waters. Version 1.5. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=quantitative_qpcr_macroalgal_nonindigenous_species_novascotia_newbrunswick_2022_2023&v=1.5For additional information please see:LeBlanc F., Belliveau V., Watson E., Coomber C., Simard N., DiBacco C., Bernier R., Gagné N. 2020. Environment DNA (eDNA) detection of marine aquatic invasive species (AIS) in Eastern Canada using a targeted species-specific qPCR approach. Management of Biological Invasions 11(2):201-217Krumhansl K.A., Brooks C.M., Lowen B., O’Brien J., Wong M., DiBacco C. Loss, resilience and recovery of kelp forests in a region of rapid ocean warming. Annals of Botany 2024 Mar 8; 133(1):73-92Brooks C.M., Krumhansl K.A. 2023. First record of the Asian Antithamnion sparsum Tokida, 1932 (Ceramiales, Rhodophyta) in Nova Scotia, Canada. BioInvasions Records 12(3):745-725.

PURPOSE:Provide early detection of newly arrived Aquatic Invasive Species (AIS) and determine the spread, establishment and spatial distribution of existing AIS within marine waters of the southern Gulf of St. Lawrence (sGSL), DFO Gulf Region boundaries (northern and eastern coastal shores of NB, Gulf shore of NS, and PEI shoreline).DESCRIPTION:DFO Science monitors for AIS in the Gulf Region. The data collected from DFO's biofouling monitoring program provides an overview of the distribution and abundance of Aquatic Invasive Species (AIS) in the Gulf Region. This information can be used by the general public, scientists and DFO managers.Monitoring program targeting aquatic invasive species (AIS). Native biofouling species are not included in this dataset. Botrylloides violaceus: Violet tunicateBotryllus schlosseri: Golden star tunicateCiona intestinalis: Vase tunicateStyela clava: Clubbed tunicateCaprella mutica*: Japanese skeleton shrimpMembranipora membranacea: Coffin box bryozoanCarcinus maenas*: European green crabCodium fragile*: Oyster thief algaJuxtacribrilina mutabilis**: Ribbed bryozoan (JCM)*indicates species that are not included as percent cover as they are not accurately captured by the sampling method, but are included as detections.**indicates species was only integrated fully into the monitoring protocol starting in 2025.Included here is a dataset of detection and percent cover data of AIS, as well as a monitoring station dataset. Environmental data collected, including from temperature loggers, are stored but not included here. PARAMETERS COLLECTED:Air and water temperature, salinity, depth, dissolved oxygen, weather conditions, list of biofouling AIS, percent cover of AIS on PVC plates, and incidental detection of other species and AIS in surrounding area. Only species data is pushed to the Open Data platform.NOTES ON QUALITY CONTROL:Each sample and species is processed and identified in a standardized fashion using standardized DFO Science AIS protocols and taxonomic references. Data is manually entered into DFO Gulf Region's AIS Science biofouling database and randomly verified for accuracy.SAMPLING METHODS:Biofouling monitoring is conducted using PVC collector plates that are deployed in the water column approximately 1 meter below the sea surface in the spring of each year. Biofouling organisms settle on these plates which are collected in the fall of the same year. Abundances of AIS are given as percent plate cover. Physico-chemical data including temperature, conductivity, and depth as well as weather conditions are noted at each geo-referenced biofouling monitoring site during initial deployment and at time of retrieval. All biofouling organisms settled on the underside of the PVC plates are noted and percent cover of each AIS is estimated. Any AIS present at the location is also noted. A HOBO temperature and light logger was deployed.USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

An Element Occurrence (EO) is an area of land and/or water in which a species or ecological community is, or was, present. An Element is either a species (or subspecies taxa) or an ecological community, the Occurrence is the documented location. The EO concept is part of NatureServe methodology. This methodology is used throughout the NatureServe network. EOs are created based on the Element Occurrence Data Standard and are a derived product developed from submitted observations. An EO should have practical conservation value for the Element as evidenced by potential continued (or historical) presence and/or regular recurrence at a given location. For Species Elements, the EO often corresponds with the local population, but when appropriate may be a portion of a population (e.g., for long distance dispersers) or may be a group of nearby populations (e.g., metapopulation). For Ecological Community Elements, the EO may represent a stand or patch of a natural community, or a cluster of stands or patches of a natural community. This dataset contains Non-sensitive EOs. Non-Sensitive EOs are locations (i.e. occurrences) of species or communities that are rare (or of conservation concern for some other reason) and for which there are no restrictions regarding public access to location data (beyond agreeing to the Terms and Conditions detailed below). This data updates on a daily basis.

The Coastal Oceanography and Ecosystem Research section (DFO Science) reviewed the presence of Cod in the Population Ecology Division (DFO Science) Ecosystem Survey trawls to describe the likelihood of presence. The survey consists of a stratified random design using a bottom trawl. This layer was created for consideration in oil spill response planning.A version of this dataset was created for the National Environmental Emergency Center (NEEC) following their data model and is available for download in the Resources section.Cite this data as: Lazin, G., Hamer, A.,Corrigan, S., Bower, B., and Harvey, C. Data of: Likelihood of presence of Atlantic Cod in Area Response Planning pilot areas. Published: June 2018. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, St. Andrews, N.B. https://open.canada.ca/data/en/dataset/af2bf6c0-481d-4445-bbc6-7a785d2a9aa9

This project was completed by the Shellfish Section in the Newfoundland and Labrador Science Branch of Fisheries and Oceans Canada (DFO), in collaboration with industry partners. The Coastal Environmental Baseline program supported the Placentia Bay portion of project work for an ongoing industry-DFO collaborative post-season trap survey for Snow Crab that was initiated in 2003 and has occurred each year. This survey is conducted by Snow Crab harvesters accompanied by at-sea observers and takes place in NAFO Divisions 2J3KLNOP4R. Historically the survey focused on commercial fishing grounds but began transitioning to a partly random stratified design in 2017. Since 2018, approximately 50% of survey stations are randomly allocated while 50% remain fixed. At each station, six (for inshore stations) or ten (for offshore stations) commercial traps are set in a fleet. To gather data on non-commercial sized Snow Crab, including females, many fleets also include one small-mesh trap. The coverage of small-mesh traps has been expanding in recent years with the aim of one small-mesh trap for every station in the coming years. Biological sampling is undertaken on at least one commercial trap and the small-mesh trap at each station. The data from this survey is incorporated into the annual stock assessment for Snow Crab in the Newfoundland and Labrador region. This record contains trap locations for Placentia Bay, and information on the types of data collected. More detailed information can be found in Pantin et al. (2022).https://publications.gc.ca/collections/collection_2023/mpo-dfo/fs70-5/Fs70-5-2022-076-eng.pdf

Polar cod (Boreogadus saida), Atlantic cod (Gadus morhua), and Greenland cod (Gadus macrocephalus) are prominent gadid species within the northwest Atlantic Ocean in terms of their ecological and socio-economic importance but it is unclear how climate-induced changes in ocean temperature may alter their distributions by the end of the century (2100). We used physiologically based species distribution models to predict how ocean warming will influence the availability of suitable habitat for early life-stages in these marine gadids. We applied CMIP5 ocean temperature projections to egg survival and juvenile growth models for Polar cod, Atlantic cod, and Greenland cod to create predicted suitability raster surfaces for these metrics across four climatology periods (1981–2005, 2026–2050, 2051–2075, 2076–2100). The analysis focused on the projected changes in temperature in ocean shelf areas where ocean depth is ≤400 m. We created an integrated habitat suitability index by combining the suitability surfaces for egg survival and growth potential to predict areas and periods where thermal conditions were suitable for both life stages. The resulting surfaces indicate that suitable thermal habitat for the juvenile life stages of all three species will shift poleward, but the magnitude of the shift and the overall area of thermally suitable habitat remaining will differ across species and life stages through time. Modelled layers are provided in NetCDF format by metric (egg survival, growth potential, habitat suitability). Data layers for Polar cod, Atlantic cod, and Greenland cod are included within each NetCDF file as variables across time. Note that in this study we refer to Gadus macrocephalus/ogac as Greenland cod since Gadus ogac is thought to be a junior synonym of Gadus macrocephalus (Carr et al., 1999). For more details on the methods and results for this analysis see Cote et al. (2021).References:Carr, S. M., Kivlichan, D. S., Pepin, P., & Crutcher, D. C. (1999). Molecular systematics of gadid fishes: implications for the biogeographic origins of Pacific species. Canadian Journal of Zoology, 77(1), 19–26. https://doi.org/10.1139/cjz-77-1-19Cote, D., Konecny, C. A., Seiden, J., Hauser, T., Kristiansen, T., & Laurel, B. J. (2021). Forecasted Shifts in Thermal Habitat for Cod Species in the Northwest Atlantic and Eastern Canadian Arctic. Frontiers in Marine Science, 8(November), 1–15. https://doi.org/10.3389/fmars.2021.764072

This project was completed by the Coastal Environmental Baseline Program (Coastal and Freshwater Ecology Section) in the Newfoundland and Labrador Science Branch of Fisheries and Oceans Canada (DFO). The nearshore fish and invertebrate community was sampled annually in Placentia Bay at 14 sites for 5 years (2018 – 2022). The objective of this program was to collect data on juvenile fish (specifically Atlantic cod) communities and habitats within Placentia Bay. The sites and timing (October) were selected based on historical sampling. There were several data types collected during each of these sampling trips. Boat-based beach seining was used to collect data on fish and invertebrate diversity, abundance, distribution, and size. Visual transects were used to identify vegetation and substrate (habitat) types and proportions at seine sites. CTD profiles (temperature, conductivity, depth, and salinity) were obtained using a castaway CTD. Finally, eDNA samples were collected at each site (2018-2021) to obtain complementary information on the presence of juvenile fish and other species present. This record contains the geographic locations of the 14 sites, and information on the timing and type of data collected at each site.

CMIST is a peer-reviewed screening-level risk assessment tool for marine invasive species (Drolet et al. 2016). It is a short questionnaire that follows the invasion process from arrival to impact and is designed so an informed assessor can evaluate one species in an assessment area in approximately one day using easily accessible information from internet databases, primary literature, and grey literature. Species can be those with an invasion history in an area or those that are candidates for future invasions.CMIST is score-based and incorporates both likelihood and impact of invasion as well as uncertainty. Questions are general to make CMIST broadly applicable to different taxa, different assessment areas, and different project goals. To date, CMIST has been tested with molluscs, tunicates, crustaceans, and polychaetes introduced or at risk of introduction to three Canadian marine ecoregions (DFO 2009). CMIST has also been successfully applied to non-indigenous freshwater fish in British Columbia with adapted guidelines (T. Therriault, pers. comm.). Upon completion, CMIST produces a risk score adjusted for the assessor’s uncertainty which, combined with information collected during the assessment, can be used to assist in management decisions. For example, in 2015, CMIST assessments were used to identify high risk invaders in three Canadian marine ecoregions (DFO 2016).

Atlantic salmon postsmolts were surveyed via surface trawling during 2001 and 2003. These data were provided to the Coastal Oceanography and Ecosystem Research section of Fisheries and Oceans Canada. These data, and information from subsequent tagging studies were considered to estimate the likelihood of presence of Atlantic salmon within the Area Response Plan regions. Atlantic salmon presence varies seasonally and this spatial information should be used in conjunction with the temporal information in the attribute table.A version of this dataset was created for the National Environmental Emergency Center (NEEC) following their data model and is available for download in the Resources section.Cite this data as: Lazin, G., Hamer, A.,Corrigan, S., Bower, B., and Harvey, C. Data of: Likelihood of presence of Atlantic Salmon in Area Response Planning pilot areas. Published: June 2018. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, St. Andrews, N.B. https://open.canada.ca/data/en/dataset/436cdf90-9d6b-4784-938b-feec48844a67

The Department of Fisheries and Oceans (DFO) Science Branch has designed a multispecies dive survey protocol to provide unbiased, coast wide monitoring of benthic invertebrate stocks (as may be required under the updated Fisheries Act) and associated habitat information for a suite of benthic marine invertebrate species (Green (Strongylocentrotus droebachiensis), Purple (S. purpuratus) and Red Sea Urchin (Mesocentrotus franciscanus), Geoduck (Panopea generosa), Giant Red Sea Cucumber (Apostichopus californicus), Northern Abalone (Haliotis kamtschatkana), and Sunflower Sea Star (Pycnopodia helianthoides)). Based on information available at this time, the proposed survey design can provide estimates of coast wide stock status for Red Sea Urchin and Giant Red Sea Cucumber, and relative abundance indices for Geoduck, Green Sea Urchin, Purple Sea Urchin, Northern Abalone and Sunflower Sea Star.The new protocol was tested through a series of pilot surveys conducted on a subset of areas of the BC coast each September from 2016-2021. Design of the pilot surveys was based on previous dive survey data and experience and demonstrated the practical feasibility of the protocol, while also gathering preliminary information to guide recommendations about the statistical design of the survey.The dataset consists of a relational database containing tables representing each component of the survey methodology. The primary component of the survey is a transect location. Along each transect, systematically spaced quadrats are sampled, and on each quadrat, substrate observations are recorded, multiple species of algae are recorded, and multiple individual invertebrates are measured or counted. The tables are linked by transect number and quadrat number.