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We have found 22 datasets for the keyword " genbank". You can continue exploring the search results in the list below.
Datasets: 106,103
Contributors: 42
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22 Datasets, Page 1 of 3
Cold-water coral DNA sequences from Eastern Canada: Part 1
Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.
FADM - Public Sustained Yield Units
The spatial representation for a Public Sustained Yield Unit, which is an area of Crown land, usually a natural topographic unit determined by drainage areas, managed for sustained yield by the Crown through the Ministry of Forests. It includes all Crown lands within the currently established boundaries of the unit and excludes federal lands, provincial parks, experimental forest reserves, gazetted watersheds and tree farm licences. Crown land designated as a public sustained yield unit under Section 6 of the Forest Act. A portion of a TSA
Manitoba COVID-19 Rapid Antigen Test Kit Distributors
Feature point layer showing COVID-19 rapid antigen test kit distribution sites in Manitoba.Feature point layer showing locations of distribution sites that currently have COVID-19 rapid antigen test kits available in Manitoba. The main purpose of this data is to provide the public with the hours of operation for these sites. Distributors include pharmacies, retail stores, and community libraries. This data is populated by Manitoba Shared Health and is updated as needed. This feature layer is used in the Manitoba COVID-19 Rapid Antigen Test Kit Availability Map and Manitoba COVID-19 Rapid Antigen Test Kit Availability application. For more information on Manitoba’s response to COVID-19, please visit the following site: https://www.gov.mb.ca/covid19/index.html Fields included [Alias (Field Name): Field description] Site Category (Site_Category): Indicates whether the site is a retail business, pharmacy, or community library Site Name (Site_Name): Official business name of the site Address (Address): Street address of the site City or Town (City_or_Town): City or town in which the site is located Postal Code (Postal_Code): Postal code that corresponds with the street address of the site Phone (Phone): Telephone number for the site French Service (French_Service): Indicates whether a site self-identified as having services available in French; if unknown, value is null Health Authority (Health_Authority): Name of the regional health authority where the site is located Hours of Operation (Hours_of_Operation): Listing of days and times that the site is open to the public Alt Hours Start (Alt_Hours_Start): Start date of when alternate hours are in effect Alt Hours End (Alt_Hours_End): End date of when alternate hours are in effect Alternate Hours of Operation (Alternate_Hours_of_Operation): Listing of alternate days and times that the site is open to the public Latitude (Latitude): Latitudinal coordinate of the site Longitude (Longitude): Longitudinal coordinate of the site
FADM - Region Compartment
The spatial representation for an Inventory Region or Compartment. Established by the Resource Inventory Branch, Inventory Regions are large drainages encompassing groups of small drainages also known as compartments. These were once used for planning purposes, similar to the current Planning Cells. They are now maintained in the files as reference for many administration boundaries
Lake Ontario tagged Atlantic salmon
Get data on Lake Ontario tagged Atlantic salmon recaptures. This dataset represents tagged adult Atlantic salmon that were released and then recaptured by anglers. These fish were used as brood stock (breeding) in the Ministry of Natural Resources and Forestry’s fish culture program. All fish were tagged with a streamer tag near the dorsal fin. Each tag has a unique number and a phone number so anglers can call and let the ministry know about their catch. The data includes: * tagging date * recapture date * tag number/colour * location released * location recaptured * days since released * distance travelled
Ontario Dam Inventory
The Ontario Dam Inventory (ODI) is an inventory of medium and large dams throughout Ontario. It uses a point-based system (x, y location) to identify each dam location. The ODI does not contain: * small dams * small water control structures * beaver dams * water crossings * road embankments * locks * falls * rapids * culverts Each dam location has an identifier that can be used to link to other dam information databases.
Registered Flu and COVID Providers 1
Registered vaccine distribution locations for the Flu and COVID-19 season that will appear on the Vaccine Provider App.
The Canadian Radiological Monitoring Network – Gross Alpha / Beta in Drinking Water
This dataset provides the results obtained by Health Canada’s Canadian Radiological Monitoring Network (CRMN) for the gross alpha and beta activity concentrations in drinking water, given in units of becquerels per liter (Bq/L). More information about the CRMN network can be found on the Health Canada website (see link below). Although water quality is a matter of provincial jurisdiction, the CRMN, in collaboration with the city of Ottawa, has been conducting a targeted program to monitor the radiological content of drinking water from two water treatment plants in Ottawa, ON. The Guidelines for Canadian Drinking Water Quality recommend screening levels of 0.5 Bq/L and 1.0 Bq/L for gross alpha and gross beta activity, respectively. The screening levels are set to reflect the most restrictive Maximum Acceptable Concentrations (MACs) for specific radionuclides in drinking water. If the screening levels are not exceeded, compliance with the guidelines can be inferred. The screening levels set out in the Guidelines for Canadian Drinking Water Quality are calculated based on annual averages of radionuclides in drinking water. Short-term exposure to levels above those recommended by these guidelines does not indicate a health risk. The measured gross alpha and gross beta activity concentrations presented here are well below the screening levels set by the Guidelines for Canadian Drinking Water Quality, with only one exception to date. This occurred February 28, 2011, and was attributable to the flushing of lead pipes at the water treatment plant. It resulted in a spike of naturally occurring lead radionuclides that was dealt with immediately by the City of Ottawa. The map shows the approximate sampling location for each monitoring station. Stations are found within the associated location range.
Base Stream and Flow Representation
The Single Line Hydrography Network (SLNET) contains all captured single line representations of hydrographic features. In addition, single line representations of polygonal features and single line arbitrary network connectors are in the file.
Time stamps
Location of timestampers.attributs:ID - Unique IdentifierNo - Number**This third party metadata element was translated using an automated translation tool (Amazon Translate).**
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