Basic biological data for all fish caught during the 2012 BSMFP expedition. Includes identification, weight, length (total, fork, and, standard), liver weight, gonad weight, sex and maturity level.

During the period of 1987 to 1994, Robin J. LeBrasseur and N. Brent Hargreaves lead a juvenile salmon predation research project in Alberni Inlet and Barkley Sound BC. This dataset contains the research survey catch data and individual fish examinations data.

PURPOSE:To characterize food webs of lake fish communities using stable isotopes, gut content morphology and DNA.DESCRIPTION:Data sets containing fish species caught in Miramichi Lake, McKiel Lake and Nashwaak Lake. PARAMETERS COLLECTED:Species counts (ecological); points (spatial)USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

An exploratory fishery was carried out in the Mackenzie River Delta between 1989 and 1993 at the request of the Inuvik Hunters and Trappers Committee to assess the commercial fishery potential in this area. Data collected during this exploratory fishery was used in two technical reports. Broad whitefish were the target species of this fishery (DFO Technical Report 2180), however other species such as northern pike, inconnu, and lake whitefish were also harvested (DFO Technical Report 2330).Biological data from broad whitefish (fork length, age, gonadosomatic index and instantaneous mortality) were analyzed to assess the impact of an exploratory fishery in the Mackenzie River Delta. The data were collected using variable mesh experimental gill nets and commercial harvesters' 139 mm (5.5”) mesh gill nets. The data suggest that the broad whitefish population might be separated, with larger mature spawners gathering in the main channels prior to spawning and smaller, immature or resting fish staying in side channels away from strong currents. From this analysis we conclude that the size and structure of the broad whitefish population(s) found in this area are stable at the current level of total harvest (commercial and subsistence combined) There may be room for increased harvests but to what level is uncertain.Biological data from northern pike, inconnu, and lake whitefish were analyzed to assess the impact of the fishery on population abundance and structure. All three by catch species support subsistence fisheries in the Mackenzie Delta. lnconnu and lake whitefish migrate substantial distances, crossing land claim borders and are likely fished by a variety of user groups. Northern pike, on the other hand tend to be non-migratory with localized populations that are mainly fished by people living in the near vicinity. A concern was that commercial fishing pressure might reduce the numbers of fish available to subsistence users. Based on trends in size and age frequency, age at maturity, sex ratio, growth rates, and mortality rates, we conclude that inconnu and lake whitefish populations in the Mackenzie Delta have remained healthy and stable at the current harvest levels, however northern pike populations showed a decrease in the proportion of older fish, possibly indicating over-fishing of local stocks. lnconnu and lake whitefish may be able to withstand increased harvest, but to what extent is unknown, given that little reliable information is available on subsistence harvest levels in this region. Increasing harvest levels of pike may be risky and we suggest that a reduction of current commercial harvest quotas be considered.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

The Great Lakes Fish Biodiversity Science Database is a compilation of fish community and habitat data from DFO Science surveys, primarily related to freshwater fishes of conservation concern in the Great Lakes basin. Data include: sampling site location, date, fish species and counts, and associated habitat information. Project-specific details including purpose/objectives and study methodology are often reported in the DFO Canadian data report of fisheries and aquatic sciences series.

An exploratory project on the taxonomic and genetic diversity of decapods in three ocean subregions (Northeast Pacific, Canadian Arctic, and Northwest Atlantic), which were sampled in 2022, was undertaken by the Arctic Working Group under the Canada-U.S. Fisheries and Climate Collaboration between Fisheries and Oceans Canada (DFO) and the National Marine Fisheries Service (NMFS) of the National Oceanic and Atmospheric Administration (NOAA). This collaboration framework aims to pool Canadian and U.S. data to explore the impacts of broad-scale climate change on marine biodiversity. In early summer 2022, a sampling protocol with the selection of targeted decapods was provided to DFO and NOAA collaborators. Targeted genera were collected from a total of 10 research programs across three ocean subregions and four marine regions. The Northeast Pacific samples were collected in the Bering Sea during the Northern Bering Sea Ecosystem and Surface Trawl Survey, and the Eastern and Northern Bering Sea Continental Shelf Bottom Trawl Survey of Groundfish and Invertebrate Fauna onboard the F/V Northwest Explorer, F/V Alaska Knight and F/V Vesteraalen. In the Western Canadian Arctic (mainly from Beaufort Sea and Amundsen Gulf), specimens were collected during DFO’s Canadian Beaufort Sea – Marine Ecosystem Assessment (CBS-MEA) survey onboard the F/V Frosti. In Eastern Canadian Arctic (mainly from Baffin Bay and Davis Strait), specimens were collected during DFO’s Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) survey onboard the CCGS Amundsen and DFO’s North Atlantic Fisheries Organization (NAFO) Subarea 0B survey onboard the R/V Tarajoq. In the Estuary and Gulf of St. Lawrence (EGSL), specimens were collected from coastal surveys (scallops, sea cucumber, snow crab, and whelk surveys) onboard the CCGS Leim and offshore during the Ecosystemic Survey onboard the CCGS Teleost. Decapods were collected from various sampling gears (benthic beam trawl, modified Atlantic Western IIA otter trawl, Bacalao trawl, shrimp trawl, Digby scallop dredge, or modified sea cucumber dredge) and identified to the lowest possible taxonomic level and photographed, when possible. All specimens were frozen at sea (n = 995). In the lab, the identifications were validated or refined with the photos and the frozen specimens. DNA was extracted for 87 specimens and a section of COI gene was amplified in order to be sequenced using Sanger method. Sequences were compared with existing data using The Basic Local Alignment Search Tool (BLAST) in the National Center for Bio-technology Information Nucleotide database (NCBI-nt, including the GenBank database) to compare scientific names, where available.The present dataset includes 391 decapod species occurrences. DNA was extracted for a subset of 87 specimens (COI gene); sequences are publicly available on BOLD data portal under project code DDAO (see supporting document "citations_references.csv" for more information).The data are presented in Darwin Core format and are separated in three files:The "Activité_décapodes_DDAO_decapods_event_en" file contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The "Occurrence_décapodes_DDAO_decapods_en" file contains the taxonomic occurrences.The "ADN_décapodes_DDAO_decapods_DNA_en" file contains the DNA derived data.For further details, please refer to the technical report available in the supporting document named "citations_references.csv". USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

The Fish Pathology Program (FPP) located at the Pacific Biological Station in Nanaimo has been assessing the health of aquatic animals since the early 1970’s. Utilizing traditional diagnostic methods, the FPP has supported internal and external clients to provide clinical data and management advice on the health of aquatic animals. The dataset contains information from diagnostic fish health cases coming from the Salmonid Enhancement Program, Research, Public and I&T submissions. Data contained in the database includes pathogen findings from submitted cases from all of the Pacific Region.The publication of The Fish Health Database will comply with public release recommendations documented in recommendation twenty two, made in volume three of the Final Report (October 2012) submitted by the Cohen Commission of Inquiry into the Decline of the Sockeye Salmon in the Fraser River.

Ichthyoplankton surveys were conducted in the Strait of Georgia (British Columbia) during 1979-1981 to ascertain the onset of fish spawning, and to explore distributional pattern and estimate total biomass of fish species.Oblique tows were made using 0.25m2 Bongos equipped with 351 micron Nitex nets of modified SCOR design. All sampling gear was black to minimize potential avoidance and resulting catch bias. The tow procedure generally followed that established by CALCOFI.This dataset contains a compilation of corrected catches of juvenile fishes, fish eggs and fish larvae by station.