This layer details Important Areas (IAs) relevant to key invertebrate species (which are not corals or sponges) in the West Coast Vancouver Island (WCVI) ecoregion. This data was mapped to inform the selection of marine Ecologically and Biologically Significant Areas (EBSA). Experts have indicated that these areas are relevant based upon their high ranking in one or more of three criteria (Uniqueness, Aggregation, and Fitness Consequences). The distribution of IAs within ecoregions is used in the designation of EBSAs.Canada’s Oceans Act provides the legislative framework for an integrated ecosystem approach to management in Canadian oceans, particularly in areas considered ecologically or biologically significant. DFO has developed general guidance for the identification of ecologically or biologically significant areas. The criteria for defining such areas include uniqueness, aggregation, fitness consequences, resilience, and naturalness. This science advisory process identifies proposed EBSAs in Canadian Pacific marine waters, specifically in the Strait of Georgia (SOG), along the west coast of Vancouver Island (WCVI, southern shelf ecoregion), and in the Pacific North Coast Integrated Management Area (PNCIMA, northern shelf ecoregion).Initial assessment of IAs in PNCIMA was carried out in September 2004 to March 2005 with spatial data collection coordinated by Cathryn Clarke. Subsequent efforts in WCVI and SOG were conducted in 2009, and may have used different scientific advisors, temporal extents, data, and assessment methods. WCVI and SOG IA assessment in some cases revisits data collected for PNCIMA, but should be treated as a separate effort.Other datasets in this series detail IAs for birds, cetaceans, coral and sponges, fish, geographic features, and other vertebrates.Though data collection is considered complete, the emergence of significant new data may merit revisiting of IAs on a case by case basis.

This layer details Important Areas (IAs) relevant to key invertebrate species (which are not corals or sponges) in the Pacific North Coast Integrated Management Area (PNCIMA). This data was mapped to inform the selection of marine Ecologically and Biologically Significant Areas (EBSA). Experts have indicated that these areas are relevant based upon their high ranking in one or more of three criteria (Uniqueness, Aggregation and Fitness Consequences). The distribution of IAs within ecoregions is used in the designation of EBSAs.Canada’s Oceans Act provides the legislative framework for an integrated ecosystem approach to management in Canadian oceans, particularly in areas considered ecologically or biologically significant. DFO has developed general guidance for the identification of ecologically or biologically significant areas. The criteria for defining such areas include uniqueness, aggregation, fitness consequences, resilience, and naturalness. This science advisory process identifies proposed EBSAs in Canadian Pacific marine waters, specifically in the Strait of Georgia (SOG), along the west coast of Vancouver Island (WCVI, southern shelf ecoregion), and in the Pacific North Coast Integrated Management Area (PNCIMA, northern shelf ecoregion).Initial assessment of IAs in PNCIMA was carried out in September 2004 to March 2005 with spatial data collection coordinated by Cathryn Clarke. Subsequent efforts in WCVI and SOG were conducted in 2009, and may have used different scientific advisors, temporal extents, data, and assessment methods. WCVI and SOG IA assessment in some cases revisits data collected for PNCIMA, but should be treated as a separate effort.Other datasets in this series detail IAs for birds, cetaceans, coral and sponges, fish, geographic features, and other vertebrates.Though data collection is considered complete, the emergence of significant new data may merit revisiting of IAs on a case by case basis.

This dataset contains the abundance (per m²) and the biomass (mg dry per m²) of macrofauna (≥ 500µm) in eelgrass and adjacent bare soft sediments, collected at sites in the Atlantic of Nova Scotia from 2009 to 2013.Cite this data as: Wong M.C. Data of Benthic invertebrates in seagrass and bare soft sediments in Atlantic Nova Scotia Published May 2020. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/05d5f46a-7f19-11ea-8a4e-1860247f53e3Publications: Wong, M. C., & Dowd, M. (2021). Functional trait complementarity and dominance both determine benthic secondary production in temperate seagrass beds. Ecosphere. 12(11), e03794. https://doi.org/10.1002/ecs2.3794Wong, M. C. (2018). Secondary Production of Macrobenthic Communities in Seagrass (Zostera marina, Eelgrass) Beds and Bare Soft Sediments Across Differing Environmental Conditions in Atlantic Canada. Estuaries and Coasts, 41, 536–548. https://doi.org/10.1007/s12237-017-0286-2

The Department of Fisheries and Oceans (DFO) Science Branch has designed a multispecies dive survey protocol to provide unbiased, coast wide monitoring of benthic invertebrate stocks (as may be required under the updated Fisheries Act) and associated habitat information for a suite of benthic marine invertebrate species (Green (Strongylocentrotus droebachiensis), Purple (S. purpuratus) and Red Sea Urchin (Mesocentrotus franciscanus), Geoduck (Panopea generosa), Giant Red Sea Cucumber (Apostichopus californicus), Northern Abalone (Haliotis kamtschatkana), and Sunflower Sea Star (Pycnopodia helianthoides)). Based on information available at this time, the proposed survey design can provide estimates of coast wide stock status for Red Sea Urchin and Giant Red Sea Cucumber, and relative abundance indices for Geoduck, Green Sea Urchin, Purple Sea Urchin, Northern Abalone and Sunflower Sea Star.The new protocol was tested through a series of pilot surveys conducted on a subset of areas of the BC coast each September from 2016-2021. Design of the pilot surveys was based on previous dive survey data and experience and demonstrated the practical feasibility of the protocol, while also gathering preliminary information to guide recommendations about the statistical design of the survey.The dataset consists of a relational database containing tables representing each component of the survey methodology. The primary component of the survey is a transect location. Along each transect, systematically spaced quadrats are sampled, and on each quadrat, substrate observations are recorded, multiple species of algae are recorded, and multiple individual invertebrates are measured or counted. The tables are linked by transect number and quadrat number.

The purpose of the survey is to document and record habitat types and associated algae and marine invertebrate species in a variety of habitat types. Transect locations are randomly selected throughout the study area, which rotates between the north and south coasts of British Columbia on a biannual basis. Transects are laid perpendicular to the shoreline. A team of two divers swim the transect with data sheets to collect habitat, algae and marine invertebrate data as detailed below in the methods section. Data is keypunched in an MS Access database that can be queried for species observations and environmental information.This dataset includes three tables pulled from the original database containing observations by species, observations by quadrat, and additional header information for each observation. All three tables can be linked by the field HKey. Three lookup tables are included as well, one for algae, one for invertebrates, and one for substrates.

This geodatabase includes hotspot maps of 1) nearshore habitat richness, 2) diversity (fish and invertebrates), and 3) biomass (using catch per unit effort of fish and invertebrates), as well as two layers showing the spatial extent of the diversity and biomass hotspot analyses. Full details and methods can be found in the Rubidge et al. 2018 CSAS Research Document 2018/053 available here or at https://waves-vagues.dfo-mpo.gc.ca/Library/40759842.pdf. These data were reviewed as part of a Canadian Science Advisory Secretariat (CSAS) regional peer review process on Nov 1-2, 2017.Habitat Richness Hotspots: Because there are no systematic surveys of nearshore species that span the entire coastline of Northern Shelf Bioregion, the nearshore habitat richness hotspots were developed as a proxy for species diversity in nearshore areas. Habitat richness was calculated from eight habitat features: eelgrass, surfgrass, canopy-forming kelp, estuaries, areas of high rugosity, and hard, mixed, and soft substrate. The number of features within 1 km x 1 km planning units was counted, and hotspots were identified using the Getis-Ord G* tool in ArcGIS. Planning units with Gi_Bin values of 3 (99% confidence) were classified as habitat richness hotspots.Diversity and Biomass Hotspots: Hotspots of fish and invertebrate diversity and biomass were developed as proxies for spatial patterns of productivity in the Northern Shelf Bioregion. Diversity (Shannon diversity) and biomass (kg/hour or count/hook/hour) were calculated from DFO synoptic trawl and outside hard-bottom longline (HBLL) survey catch records. The outside HBLL survey was previously referred to as Pacific Halibut Management Area (PHMA) survey. The synoptic trawl and HBLL surveys have complementary spatial coverage, with the HBLL surveys occurring in more coastal areas (20–260 m) and the synoptic trawl surveys occurring on deeper shelf areas (50–1300 m). Hotspots were identified using the Getis-Ord G* tool in ArcGIS for five separate analyses: fish biomass (trawl), fish diversity (trawl), fish diversity (longline), invertebrate biomass (trawl), and invertebrate diversity (trawl). Using the Minimum Bounding Geometry Tool, convex hull polygons were drawn around groups of hotspot points (Gi_Bin values of 1, 2, or 3; confidence ≥90%) containing 10 or more points. The resulting polygons were then buffered by 1 km and manually edited where needed to exclude any large areas of the polygons that did not include hotspot points.

An exploratory project on the taxonomic and genetic diversity of decapods in three ocean subregions (Northeast Pacific, Canadian Arctic, and Northwest Atlantic), which were sampled in 2022, was undertaken by the Arctic Working Group under the Canada-U.S. Fisheries and Climate Collaboration between Fisheries and Oceans Canada (DFO) and the National Marine Fisheries Service (NMFS) of the National Oceanic and Atmospheric Administration (NOAA). This collaboration framework aims to pool Canadian and U.S. data to explore the impacts of broad-scale climate change on marine biodiversity. In early summer 2022, a sampling protocol with the selection of targeted decapods was provided to DFO and NOAA collaborators. Targeted genera were collected from a total of 10 research programs across three ocean subregions and four marine regions. The Northeast Pacific samples were collected in the Bering Sea during the Northern Bering Sea Ecosystem and Surface Trawl Survey, and the Eastern and Northern Bering Sea Continental Shelf Bottom Trawl Survey of Groundfish and Invertebrate Fauna onboard the F/V Northwest Explorer, F/V Alaska Knight and F/V Vesteraalen. In the Western Canadian Arctic (mainly from Beaufort Sea and Amundsen Gulf), specimens were collected during DFO’s Canadian Beaufort Sea – Marine Ecosystem Assessment (CBS-MEA) survey onboard the F/V Frosti. In Eastern Canadian Arctic (mainly from Baffin Bay and Davis Strait), specimens were collected during DFO’s Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) survey onboard the CCGS Amundsen and DFO’s North Atlantic Fisheries Organization (NAFO) Subarea 0B survey onboard the R/V Tarajoq. In the Estuary and Gulf of St. Lawrence (EGSL), specimens were collected from coastal surveys (scallops, sea cucumber, snow crab, and whelk surveys) onboard the CCGS Leim and offshore during the Ecosystemic Survey onboard the CCGS Teleost. Decapods were collected from various sampling gears (benthic beam trawl, modified Atlantic Western IIA otter trawl, Bacalao trawl, shrimp trawl, Digby scallop dredge, or modified sea cucumber dredge) and identified to the lowest possible taxonomic level and photographed, when possible. All specimens were frozen at sea (n = 995). In the lab, the identifications were validated or refined with the photos and the frozen specimens. DNA was extracted for 87 specimens and a section of COI gene was amplified in order to be sequenced using Sanger method. Sequences were compared with existing data using The Basic Local Alignment Search Tool (BLAST) in the National Center for Bio-technology Information Nucleotide database (NCBI-nt, including the GenBank database) to compare scientific names, where available.The present dataset includes 391 decapod species occurrences. DNA was extracted for a subset of 87 specimens (COI gene); sequences are publicly available on BOLD data portal under project code DDAO (see supporting document "citations_references.csv" for more information).The data are presented in Darwin Core format and are separated in three files:The "Activité_décapodes_DDAO_decapods_event_en" file contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The "Occurrence_décapodes_DDAO_decapods_en" file contains the taxonomic occurrences.The "ADN_décapodes_DDAO_decapods_DNA_en" file contains the DNA derived data.For further details, please refer to the technical report available in the supporting document named "citations_references.csv". USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

This layer details Important Areas (IAs) relevant to key invertebrate species (which are not corals or sponges) in the Strait of Georgia (SOG) ecoregion. This data was mapped to inform the selection of marine Ecologically and Biologically Significant Areas (EBSA). Experts have indicated that these areas are relevant based upon their high ranking in one or more of three criteria (Uniqueness, Aggregation, and Fitness Consequences). The distribution of IAs within ecoregions is used in the designation of EBSAs.Canada’s Oceans Act provides the legislative framework for an integrated ecosystem approach to management in Canadian oceans, particularly in areas considered ecologically or biologically significant. DFO has developed general guidance for the identification of ecologically or biologically significant areas. The criteria for defining such areas include uniqueness, aggregation, fitness consequences, resilience, and naturalness. This science advisory process identifies proposed EBSAs in Canadian Pacific marine waters, specifically in the Strait of Georgia (SOG), along the west coast of Vancouver Island (WCVI, southern shelf ecoregion), and in the Pacific North Coast Integrated Management Area (PNCIMA, northern shelf ecoregion).Initial assessment of IA's in PNCIMA was carried out in September 2004 to March 2005 with spatial data collection coordinated by Cathryn Clarke. Subsequent efforts in WCVI and SOG were conducted in 2009, and may have used different scientific advisors, temporal extents, data, and assessment methods. WCVI and SOG IA assessment in some cases revisits data collected for PNCIMA, but should be treated as a separate effort.Other datasets in this series detail IAs for birds, cetaceans, coral and sponges, fish, geographic features, and other vertebrates.Though data collection is considered complete, the emergence of significant new data may merit revisiting of IA's on a case by case basis.

The Eastern Shore Islands Area of Interest (AOI) is a large coastal AOI in the Maritimes Region spanning 2089 km2 and extending along 100 km of coastline. While much of the data that led to the area's designation as an AOI was collected in the nearshore coastal areas (i.e., seagrass and kelp distributions, coastal bird nesting sites, herring spawning areas), there is less information available on the fish and invertebrate community structure in offshore areas of the AOI. Current offshore sampling effort is primarily focused in the Summer multi-species RV survey which covers the Scotian Shelf but does not sample within the depth range covered by the AOI (~ <100m depth). Environmental DNA (eDNA) offers a promising approach for biodiversity monitoring that has been gaining increased attention in the marine realm. Through sampling relatively small volumes of water (~1-5 L), eDNA can capture of millions of fragments of eDNA on filters that can then be sequenced to identify the species composition of an area. eDNA metabarcoding is based on the foundations of DNA barcoding, where species are identified solely by their unique DNA sequences. eDNA metabarcoding relies on a comprehensive reference library of gene sequences from known species to rapidly identify which species are captured in every water sample. This method is relatively simple, efficient (with the ability to characterize both fish and invertebrate diversity), and non-invasive, meaning there is no disruption of sensitive benthic habitats or need to dissect tissue samples from captured fish and invertebrates. eDNA has been shown to be comparable to other biodiversity censusing techniques and has the potential to rapidly conduct biodiversity surveys over a relatively large area, such as the Eastern Shore Islands AOI. Our eDNA sampling in the Eastern Shore Islands targets fish and invertebrates using multiple genetic markers (e.g., 12S and COI) to obtain baseline information on these communities across transects spanning from inshore to offshore in the AOI. Over time, we will investigate changes in species richness and community composition using annual eDNA surveys as an ongoing monitoring tool for this coastal region. Cite this data as: Jeffery, N.W. Environmental DNA Monitoring in the Eastern Shore Islands Area of Interest - Offshore. Published: April 2026. Coastal Ecosystems Science Division, Maritimes Region, Fisheries and Oceans Canada, Dartmouth NS.

The Adaptive Management Framework for Yukon placer mining is complemented by traditional knowledge and monitoring of water quality objectives, aquatic health, and economic health. The aquatic health monitoring program is governed by the Aquatic Health Monitoring Protocol. The Protocol describes the locations, timing, frequency and methods employed during sampling, as well as the methods used to analyze sampling data. The Reference Condition Approach (RCA) is the method chosen for assessing the health of freshwater ecosystems in the Yukon. One RCA model was developed for bioassessment based upon benthic macroinvertebrates, and a second model was developed to assess the diversity of fish species. The RCA model for invertebrates relies upon 224 reference sites collected over the period 2004 to 2010 by the University of Western Ontario, Fisheries and Oceans Canada, and Yukon G overnment, using the same standard protocol. The invertebrate data set was analyzed at the family level. There are two fundamental steps in the process of developing the predictive model. The first is to classify the reference sites based on their biological characteristics. This requires defining a number of community types based on the taxonomic composition. The second step is to determine a subset of habitat attributes that are associated with those community types. Following this step the number and type of organisms expected to occur at any given site can be determined from habitat attributes. For more information, contact the Yukon Placer Secretariat.Distributed from [GeoYukon](https://yukon.ca/geoyukon) by the [Government of Yukon](https://yukon.ca/maps) . Discover more digital map data and interactive maps from Yukon's digital map data collection.For more information: [geomatics.help@yukon.ca](mailto:geomatics.help@yukon.ca)