Benthic Marine Ecounits in coastal and offshore British Columbia. Benthic ecounits are intended to describe the sea bed and nearshore. Seven variables were selected to derive benthic ecounits: 1. Depth; 2. Slope; 3. Relief; 4. Temperature; 5. Exposure; 6. Current and 7. Substrate. CRIMS is a legacy dataset of BC coastal resource data that was acquired in a systematic and synoptic manner from 1979 and was intermittently updated throughout the years. Resource information was collected in nine study areas using a peer-reviewed provincial Resource Information Standards Committee consisting of DFO Fishery Officers, First Nations, and other subject matter experts. There are currently no plans to update this legacy data.

Fisheries and Oceans Canada (DFO) selected the Manicouagan Peninsula area as a site of interest for the possible establishment of a Marine Protected Area (MPA) in 1998. With this in mind, DFO conducted surveys to assess benthic biodiversity between 2006 and 2008. During the various campaigns, more than a hundred stations were sampled using various tools to target epibenthic and endobenthic species. Various tools were used during the three years of sampling, including an SM (Smith-McIntyre) grab with an area of 0.1 m² and a height of 20 cm, an IKU-type grab with a surface area of 1.5 m² and a height of 50 cm, and a hydraulic dredge measuring 1.36 m wide and 0.66 m high with a net mesh size of 2.3 cm. As the dredge clogged fairly quickly, small organisms were collected in abundance. In 2006 and 2007, a camera mounted on a sled pulled by a boat along transects of varying lengths depending on the sampling campaign collected images (3,008 x 2,000 pixels) every 10 seconds, representing a seabed area of 40 x 40 cm.The data are presented in Darwin Core format in the file “donnees_manicouagan_data” The available data include taxonomic identifications of samples (individuals collected or identified in images) as well as metadata related to stations, including sampling date, depth, and geographic coordinates. Please note that the depths are for informational purposes only, since the bathymetry is very steep in this region.Further details on sampling can be found in the following report: Provencher, L et Nozères, C. 2011. Protocole de suivi des communautés benthiques de la zone de protection marine Manicouagan. Secr. can. de consult. sci. du MPO. Doc. de rech. 2011/051. iv +25 p. https://waves-vagues.dfo-mpo.gc.ca/library-bibliotheque/344357.pdf

PURPOSE:In this study, we examined the structure and function of the Southampton Island marine food web across 149 species of benthic and pelagic invertebrates, fishes, marine mammals and seabirds collected from 2016 to 2019, to provide a baseline for future studies that aim to quantify temporal changes in food web structuring. More specifically,we used a multi-biomarker approach combining stable isotopes and HBIs to: (i) determine the vertical trophic structure of the marine food web, (ii) investigate the contribution of benthic and pelagic-derived prey to the higher trophic level species of the Arctic food web, and (iii) determine the role of ice algae and phytoplankton carbon source use across different trophic levels and compartments (pelagic and benthic). By shedding new light on the functioning of the Southampton Island food web and specifically how the contribution of ice algae and benthic habitat shapes its structure, these results will be relevant to adaptive management and conservation initiatives implemented in response to anthropogenic stressors and climate change. DESCRIPTION:Climate-driven alterations of the marine environment are most rapid in Arctic and subarctic regions, including Hudson Bay in northern Canada, where declining sea ice, warming surface waters and ocean acidification are occurring at alarming rates. These changes are altering primary production patterns that will ultimately cascade up through the food web. Here, we investigated (i) the vertical trophic structure of the Southampton Island marine ecosystem in northern Hudson Bay, (ii) the contribution of benthic and pelagic-derived prey to the higher trophic level species, and (iii) the relative contribution of ice algae and phytoplankton derived carbon in sustaining this ecosystem. For this purpose, we measured bulk stable carbon, nitrogen and sulfur isotope ratios as well as highly branched isoprenoids in samples belonging to 149 taxa, including invertebrates, fishes, seabirds and marine mammals. We found that the benthic invertebrates occupied 4 trophic levels and that the overall trophic system went up to an average trophic position of 4.8. The average δ34S signature of pelagic organisms indicated that they exploit both benthic and pelagic food sources, suggesting there are many interconnections between these compartments in this coastal area. The relatively high sympagic carbon dependence of Arctic marine mammals (53.3 ± 22.2 %) through their consumption of benthic invertebrate prey, confirms the important role of the benthic subweb for sustaining higher trophic level consumers in the coastal pelagic environment. Therefore, a potential decrease in the productivity of ice algae could lead to a profound alteration of the benthic food web and a cascading effect on this Arctic ecosystem.Collaborators:Centre for Earth Observation Science, University of Manitoba, Winnipeg, Manitoba, Canada - R´emi Amiraux, C.J. Mundy, Jens K. Ehn, Z.A. Kuzyk.Quebec-Ocean, Sentinel North and Takuvik, Biology Department, Laval University, Quebec, Quebec, Canada - Marie Pierrejean.Scottish Association for Marine Science, Oban, UK - Thomas A. Brown.Department of Natural Resource Sciences, McGill University, Ste. Anne de Bellevue, Quebec, Canada - Kyle H. Elliott.Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada - Steven H. Ferguson, Cory J.D. Matthews, Cortney A. Watt, David J. Yurkowski.School of the Environment, University of Windsor, Windsor, Ontario, Canada - Aaron T. Fisk.Science and Technology Branch, Environment and Climate Change Canada, Ottawa, Ontario, Canada - Grant Gilchrist.College of Fisheries and Ocean Sciences, University of Alaska Fairbanks, Fairbanks, AK, USA - Katrin Iken.Department of Earth Sciences, University of New Brunswick, Fredericton, NB, Canada - Audrey Limoges.Department of Integrative Biology, University of Windsor, Windsor, Ontario, Canada - Oliver P. Love, Wesley R. Ogloff.Department of Arctic Biology, The University Centre in Svalbard, Longyearbyen, Norway - Janne E. Søreide.

Marine classification schemes based on abiotic surrogates often inform regional marine conservation planning in lieu of detailed biological data. However, theses chemes may poorly represent ecologically relevant biological patterns required for effective design and management strategies. We used a community-level modeling approach to characterize and delineate representative mesoscale (tens to thousands of kilometers) assemblages of demersal fish and benthic invertebrates in the North-west Atlantic. Hierarchical clustering of species occurrence data from four regional annual multispecies trawl surveys revealed three to six groupings (predominant assemblage types) in each survey region, broadly associated with geomorphic and oceanographic features. Indicator analyses identified 3–34 emblematic taxa of each assemblage type. Random forest classifications accurately predicted assemblage dis-tributions from environmental covariates (AUC > 0.95) and identified thermal limits (annual minimum and maximum bottom temperatures) as important pre-dictors of distribution in each region. Using forecasted oceanographic conditions for the year 2075 and a regional classification model, we projected assemblage dis-tributions in the southernmost bioregion (Scotian Shelf-Bay of Fundy) under ahigh emissions climate scenario (RCP 8.5). Range expansions to the north eastare projected for assemblages associated with warmer and shallower waters of the Western Scotian Shelf over the 21st century as thermal habitat on the rela-tively cooler Eastern Scotian Shelf becomes more favorable. Community-level modeling provides a biotic-informed approach for identifying broadscale ecolog-ical structure required for the design and management of ecologically coherent, representative, well-connected networks of Marine Protected Areas. When com-bined with oceanographic forecasts, this modeling approach provides a spatial tool for assessing sensitivity and resilience to climate change, which can improve conservation planning, monitoring, and adaptive management.Cite this data as: O'Brien, J.M., Stanley, R.R.E., Jeffery, N.W., Heaslip, S.W., DiBacco, C., and Wang, Z. Demersal fish and benthic invertebrate assemblages in the Northwest Atlantic.Published: December 2024. Coastal Ecosystems Science Division, Maritimes region, Fisheries and Oceans Canada, Dartmouth NS.https://open.canada.ca/data/en/dataset/14d55ea5-b17d-478c-b9ee-6a7c04439d2b

This dataset contains the abundance (per m²) and the biomass (mg dry per m²) of macrofauna (≥ 500µm) in eelgrass and adjacent bare soft sediments, collected at sites in the Atlantic of Nova Scotia from 2009 to 2013.Cite this data as: Wong M.C. Data of Benthic invertebrates in seagrass and bare soft sediments in Atlantic Nova Scotia Published May 2020. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/05d5f46a-7f19-11ea-8a4e-1860247f53e3Publications: Wong, M. C., & Dowd, M. (2021). Functional trait complementarity and dominance both determine benthic secondary production in temperate seagrass beds. Ecosphere. 12(11), e03794. https://doi.org/10.1002/ecs2.3794Wong, M. C. (2018). Secondary Production of Macrobenthic Communities in Seagrass (Zostera marina, Eelgrass) Beds and Bare Soft Sediments Across Differing Environmental Conditions in Atlantic Canada. Estuaries and Coasts, 41, 536–548. https://doi.org/10.1007/s12237-017-0286-2

The North Shore of the Lower Estuary (Upper North Shore, Quebec) is a productive coastal system where many commercial species of benthic invertebrates are fished in the infralittoral (10-20 m) and circalittoral (20-50 m) zone. However, little data exist on the biodiversity of non-commercial species and the environmental characteristics of the benthic habitat in this area. Two scientific surveys were conducted in 2018 and 2019 to address this knowledge gap by developing a framework of biodiversity and environmental (water column and seafloor) data taking that will be used to determine the baseline state of the benthic ecosystem in this region.Surveys were conducted in 2018 (August 11-14) and 2019 (July 30-August 5) in the Upper North Shore region (between the towns of Forestville and Godbout). Surveys followed a fixed sampling design of eight transects perpendicular to bathymetry with stations at 10 m depth intervals in a bathymetry range of 10-50 m for a total of approximately 40 stations per survey. Specimens were collected with a beam trawl with an opening of 2.8 m. The hauls were made at a target speed of 2 knots and a target duration of 7 minutes. Start and end positions were recorded to calculate the distance traveled on each tow using the geosphere library of R. The average tow distance was approximately 425 m. The area covered at each tow was the product of the trawl opening and the distance traveled. The three files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "event_information" file includes generic event information, including date and location. The "additional_information_event_and_occurrence" file includes sample size, sampling protocol and sampling effort, among others. The "taxon_occurrence" file includes the taxonomy of the species observed, identified to the species or lowest possible taxonomic level. For abundance and biomass estimates, contact Virginie Roy (virginie.roy@dfo-mpo.gc.ca). For quality controls, all taxonomic names were checked against the World Register of Marine Species (WoRMS) to match recognized standards. The WoRMS match was placed in the "scientificNameID" field of the occurrence file. Special cases were noted in "identificationRemarks" and selected specimens were confirmed using field photos. Data quality checks were performed using the R obistools and worrms libraries. All sampling locations were spatially validated.This project was funded by DFO Coastal Environmental Baseline Program under Canada’s Oceans Protection Plan. This initiative aims to acquire environmental baseline data contributing to the characterization of important coastal areas and to support evidence-based assessments and management decisions for preserving marine ecosystems.

Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes.Cite this data as: Jeffery, N., Rubidge, E., Abbott, C., Westfall, K., Stanley, R. (2024): Data of: eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment.Published: August 2024. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/43a91ba7-8025-4330-88db-db14022d729d

The Department of Fisheries and Oceans (DFO) Science Branch has designed a multispecies dive survey protocol to provide unbiased, coast wide monitoring of benthic invertebrate stocks (as may be required under the updated Fisheries Act) and associated habitat information for a suite of benthic marine invertebrate species (Green (Strongylocentrotus droebachiensis), Purple (S. purpuratus) and Red Sea Urchin (Mesocentrotus franciscanus), Geoduck (Panopea generosa), Giant Red Sea Cucumber (Apostichopus californicus), Northern Abalone (Haliotis kamtschatkana), and Sunflower Sea Star (Pycnopodia helianthoides)). Based on information available at this time, the proposed survey design can provide estimates of coast wide stock status for Red Sea Urchin and Giant Red Sea Cucumber, and relative abundance indices for Geoduck, Green Sea Urchin, Purple Sea Urchin, Northern Abalone and Sunflower Sea Star.The new protocol was tested through a series of pilot surveys conducted on a subset of areas of the BC coast each September from 2016-2021. Design of the pilot surveys was based on previous dive survey data and experience and demonstrated the practical feasibility of the protocol, while also gathering preliminary information to guide recommendations about the statistical design of the survey.The dataset consists of a relational database containing tables representing each component of the survey methodology. The primary component of the survey is a transect location. Along each transect, systematically spaced quadrats are sampled, and on each quadrat, substrate observations are recorded, multiple species of algae are recorded, and multiple individual invertebrates are measured or counted. The tables are linked by transect number and quadrat number.

This inventory, conducted from September 26th to October 3th, 2019, aimed to describe the community structure of macroalgae and benthic macroinvertebrates of five small estuaries of the Upper North Shore of Quebec, namely Barthélemy Bay and the Colombier, Mistassini, Franquelin and Saint-Nicolas rivers. This inventory is part of a doctoral study of Valentine Loiseau on the global changes in the St. Lawrence system, mainly the study of marine benthic communities in response to changes of salinity, to ensure proper management of the environment in the face of future changes. The main objective is to describe the structure and the levels of specific diversities of mediolittoral communities of benthic macroinvertebrates and macroalgae along a salinity gradient. These five small estuaries were selected because of their similar size, hard substrates and easy access. Three levels of hypoosmotic stress (low, medium, high) and one control level (seawater) were used for each of the selected estuaries, with eight quadrats per stress level. Quadrat positions were randomly selected but had to meet two criteria: (1) regular height in the foreshore to control the influence of other stresses (temperature, exposure); and (2) presence of at least one macroalga to maintain homogeneity. A percentage cover by macroalgal and macroinvertebrate species was estimated, and then all organisms were weighed by species and size group. The salinity of the nearest water point was measured at mid-tide with a portable refractometer and a Castaway-type CTD (Conductivity-Temperature-Density) probe. The inventory was done using a stratified random sampling design and the sampling unit was a quadrat measuring 25 x 25 cm. The three files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "event_information" file includes the generic information of the quadrat, including date and location. The "additional_information_event_and_occurrence" file includes salinity and substrate type of the quadrat, as well as the total weight of all individuals of the same species caught in the quadrat extrapolated to one square metre of surface. For nudibranchs and barnacles, weight was estimated from the size of the individuals so that they were not removed from the environment. The "taxon_occurrence" file includes the taxonomic inventory of macroalgal and benthic macroinvertebrate species observed in the quadrat, identified to the lowest possible species or taxonomic level and biomass by identified species.For quality control, organisms were identified on the field using the following guide: Chabot, Robert et Anne Rossignol. 2003. Algues et faune du littoral du Saint-Laurent maritime : Guide d'identification. Institut des Sciences de la mer de Rimouski, Rimouski ; Pêches et Océans Canada (Institut Maurice-Lamontagne), Mont-Joli. 113 pages. The taxonomy was checked against the World Register of Marine Species (WoRMS) to match recognized standards and using the R obistools and worrms libraries. The WoRMS match was placed in the "scientificNameID" field of the occurrence file. All sample locations were spatially validated. This project was funded by DFO Coastal Environmental Baseline Program under Canada’s Oceans Protection Plan. This initiative aims to acquire environmental baseline data contributing to the characterization of important coastal areas and to support evidence-based assessments and management decisions for preserving marine ecosystems.

Towfish (sidescan and video) and echo sounder surveys were utilized to examine bottom type and macrophyte cover within the area of two coastal marine finfish aquaculture sites, one in New Brunswick (Welch Cove) and one in Nova Scotia (Jordan Bay). Both towfish and echo sounder data could be used independently of one another. However, the towfish data were very useful for ground truthing echo sounder based classifications. All survey data were placed into a GIS which could be used to answer management questions such as the placement of cages at sites, benthic impacts and baseline conditions to determine long term changes.Cite this data as: Vandermeulen H. Data of: Exploratory Video-Sidescan and Echosounder Survey of Welch Cove. Published: June 2021. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/0083e317-8bb5-492a-8348-c021e183f307