PURPOSE:The purpose of this work was to determine (1) how the population genomic structure relates to gut microbiota composition of White Hake (Urophycis tenuis), and (2) whether microbiota community variation provides complementary insights into population structure of this species in eastern Canada.DESCRIPTION:Integrating host-associated microbiota with genomic approaches offers an opportunity to better understand the multiple biological dimensions shaping population structure in marine fishes. A clear understanding of population structure and dynamics is essential for informed fisheries management and conservation decisions; however, while genomic approaches have greatly improved our ability to delineate biological populations, they provide only a partial representation of biological structure, as patterns of differentiation reflect both historical divergence and contemporary ecological conditions. Host-associated microbiota can influence population-level ecological processes by contributing functional, potentially heritable variation that shapes host phenotype and fitness.In this study, we combined genotyping-by-sequencing with 16S rRNA gene amplicon sequencing to examine how population genomic structure relates to gut microbiota composition and to evaluate whether microbiota community variation provides complementary insights into population structure in White Hake (Urophycis tenuis) in eastern Canada. Genomic analyses identified two populations with greater spatial overlap than previously reported. Variation partitioning revealed that host genetics explained a negligible proportion of microbiota variation compared to environmental factors and fish length, suggesting that ontogenetic shifts in habitat use and resource acquisition influence gut microbiota composition. Several taxa were differentially abundant among fish length categories used as a proxy for diet, including taxa with chitin-degrading potential such as Photobacterium and Lachnospirales, which were enriched in smaller fish known to consume a crustacean-dominated diet. Together, these results indicate that gut microbiota composition in White Hake primarily reflects ecological and life-history processes rather than host population structure. PARAMETERS COLLECTED:Environmental parameters were also collected at most sampling sites, including depth, water temperature, oxygen and salinity levels.PHYSICAL SAMPLE DETAILS:Fin samples were collected to characterize the fish genotypes. Intestine (rectum) samples were collected to study fish gut microbiome.SAMPLING METHODS:In 2022 and 2023, White Hake were sampled during Fisheries and Oceans Canada (DFO) annual bottom trawl ecosystem monitoring surveys.USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

BiologicEcologic ISO Feature Dataset symbolization and publication. September 5, 2017.

Historical finds of Profenusa thomsoni

In 2018, Fisheries and Oceans Canada initiated the Multidisciplinary Arctic Program (MAP) – Last Ice, the first ecosystem study of the poorly characterized region of the Lincoln Sea in the Marine Protected Area of Tuvaijuittuq, where multiyear ice still resides in the Arctic Ocean. MAP-Last Ice takes a coordinated approach to integrate the physical, biochemical, and ecological components of the sea ice-ocean connected ecosystem and its response to climate and ocean forcings. The cross-disciplinary program establishes baseline ecological knowledge for Tuvaijuittuq and, in particular, for its unique multiyear ice ecosystem. The database provides baseline data on the abundance of bacteria and viruses in multi- and first-year ice and in surface waters of the Lincoln Sea in Tuvaijuittuq, and their relation to bio-physical conditions. The data were collected during the 2018 spring field campaign of the MAP-Last Ice Program, at an ice camp offshore of Canadian Forces Station (CFS) Alert.

Zones were created to manage the potential spread of fish pathogens. This product requires the use of geographic information system (GIS) software.

Description:Biophysical Units: Under the Pacific Marine Ecological Classification System (PMECS; DFO 2016; Rubidge et al. 2016), biophysical units are areas of distinct physiographic and oceanographic conditions and processes that shape species composition at spatial extents of 1000s of km. Geomorphic units:Geomorphic units or geozones are discrete geomorphological structures at the scale of 100s of km that are assumed to have distinctive biological assemblages (e.g., plateaus, ridges, seamounts, canyons). Although the spatial scale of geomorphic units is nested within biophysical units, a single geomorphic unit such as a trough may span more than one biophysical unit. The following 5 layers are included in this geodatabase:1. Biophysical_Units_L4A - Predicted PMECS Biophysical Units (Level 4A) output from the random forest analysis2. Biophysical_Units_L4B - Predicted PMECS Biophysical Units (Level 4B) output from the random forest analysis3. Biophysical_Units_ProbAssign_L4AB - Layer showing the probability that a grid cell was assigned to a given biophysical unit in the final random forest predictive modelling step4. Cluster_L4AB - Layer showing the output of species assemblage cluster analysis5. Geomorphic_Units - Geomorphic units for the BC coast that combines geomorphic units produced by Rubidge et al. 2016) and Proudfoot and Robb (2022).Methods:Biophysical Units:Rubidge et al. (2016) used a two-step process to identify biophysical units in British Columbia. First, a cluster analysis based on the similarity of species composition was used to group sites with similar species into distinct biological assemblages. Second, a random forest analysis was used to identify environmental correlates of the biological assemblages identified by the cluster analysis and to predict and assign the biological assemblage present in areas with too few biological data. Two different similarity thresholds were used to identify two levels (4A, 4B) of biophysical units; see Rubidge et al. (2016) for details. Indicator species for each assemblage (biophysical unit) were also identified.Geomorphic units:Rubidge et al. (2016) used the benthic terrain modeller (BTM) tool with broad and fine-scale benthic positioning index (BPI) parameters to define geomorphic units on the continental shelf in the Northern Shelf Bioregion and the continental slope in both the Northern Shelf Bioregion and Southern Shelf Bioregion. In 2022, geomorphic units were produced for the Strait of Georgia and Southern Shelf Bioregions following the same methods as Rubidge et al. (2016) (Proudfoot and Robb 2022). The geomorphic units produced as part of the PMECS process were merged with the geomorphic units produced for the Strait of Georgia and Southern Shelf bioregions to produce a continuous spatial data product representing geomorphic units for the Canadian Pacific continental shelf and slope. After merging, the geomorphic units produced in 2016 were unchanged (i.e., they are consistent with the original geomorphic units described in Rubidge et al. 2016).Data Sources:From Rubidge et al. (2016): Species data was taken from Fisheries and Oceans Canada (DFO) standardized fisheries-independent research surveys: groundfish trawl and long-line (2003-2013), Tanner Crab trawl and trap (2000–2006), and Dungeness Crab trap (2000–2014). Environmental data came from NASA, the Canadian Hydrographic Service, Fisheries and Oceans Canada, Bio-ORACLE, and elsewhere (details in Rubidge et al. 2016). From Proudfoot and Robb (2022): bathymetry data came from Natural Resources Canada (details in Proudfoot and Robb 2022).Uncertainties:The data is intended for use at the bioregional scale, and caution should be used for finer-scale analyses.

Programs that perform a laboratory analysis of body fluids and tissue to determine the presence of irregularities or to identify unknown substances submitted for analysis. Definition is protected by Copyright by Information and Referral Federal of Los Angeles County, Inc (https://211taxonomy.org/subscriptions/#agreement)

An exploratory project on the taxonomic and genetic diversity of decapods in three ocean subregions (Northeast Pacific, Canadian Arctic, and Northwest Atlantic), which were sampled in 2022, was undertaken by the Arctic Working Group under the Canada-U.S. Fisheries and Climate Collaboration between Fisheries and Oceans Canada (DFO) and the National Marine Fisheries Service (NMFS) of the National Oceanic and Atmospheric Administration (NOAA). This collaboration framework aims to pool Canadian and U.S. data to explore the impacts of broad-scale climate change on marine biodiversity. In early summer 2022, a sampling protocol with the selection of targeted decapods was provided to DFO and NOAA collaborators. Targeted genera were collected from a total of 10 research programs across three ocean subregions and four marine regions. The Northeast Pacific samples were collected in the Bering Sea during the Northern Bering Sea Ecosystem and Surface Trawl Survey, and the Eastern and Northern Bering Sea Continental Shelf Bottom Trawl Survey of Groundfish and Invertebrate Fauna onboard the F/V Northwest Explorer, F/V Alaska Knight and F/V Vesteraalen. In the Western Canadian Arctic (mainly from Beaufort Sea and Amundsen Gulf), specimens were collected during DFO’s Canadian Beaufort Sea – Marine Ecosystem Assessment (CBS-MEA) survey onboard the F/V Frosti. In Eastern Canadian Arctic (mainly from Baffin Bay and Davis Strait), specimens were collected during DFO’s Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) survey onboard the CCGS Amundsen and DFO’s North Atlantic Fisheries Organization (NAFO) Subarea 0B survey onboard the R/V Tarajoq. In the Estuary and Gulf of St. Lawrence (EGSL), specimens were collected from coastal surveys (scallops, sea cucumber, snow crab, and whelk surveys) onboard the CCGS Leim and offshore during the Ecosystemic Survey onboard the CCGS Teleost. Decapods were collected from various sampling gears (benthic beam trawl, modified Atlantic Western IIA otter trawl, Bacalao trawl, shrimp trawl, Digby scallop dredge, or modified sea cucumber dredge) and identified to the lowest possible taxonomic level and photographed, when possible. All specimens were frozen at sea (n = 995). In the lab, the identifications were validated or refined with the photos and the frozen specimens. DNA was extracted for 87 specimens and a section of COI gene was amplified in order to be sequenced using Sanger method. Sequences were compared with existing data using The Basic Local Alignment Search Tool (BLAST) in the National Center for Bio-technology Information Nucleotide database (NCBI-nt, including the GenBank database) to compare scientific names, where available.The present dataset includes 391 decapod species occurrences. DNA was extracted for a subset of 87 specimens (COI gene); sequences are publicly available on BOLD data portal under project code DDAO (see supporting document "citations_references.csv" for more information).The data are presented in Darwin Core format and are separated in three files:The "Activité_décapodes_DDAO_decapods_event_en" file contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The "Occurrence_décapodes_DDAO_decapods_en" file contains the taxonomic occurrences.The "ADN_décapodes_DDAO_decapods_DNA_en" file contains the DNA derived data.For further details, please refer to the technical report available in the supporting document named "citations_references.csv". USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Zooplankton and ichthyoplankton data are archived in the Institute of Ocean Sciences (IOS) Zooplankton Database. The data available spans from 1980 to 2018 and is an extraction of vertical net hauls as biomass by major taxa collected during surveys conducted in the oceanic and coastal waters of the Northeast Pacific Ocean. The majority of vertical net hauls in this data set were collected from 10 metres above the sea floor or an approximate maximum depth of 250 metres. For further data requests, please use the contact information provided.

Zooplankton samples were collected at Ocean Station "P" (50.0000, -145.0000) from 1956 to 1980, and were analyzed to various levels of taxonomic resolution over the years. Although summaries of these data have been previously published ((LeBrasseur 1965) and (Fulton 1978, 1983)) the detailed species data have never been published. This detailed dataset contains total zooplankton wet weights/m3 for the whole period of 1956 to 1980, as well as densities (numbers/m3) for five major taxa (copepods, chaetognaths, euphausiids, amphipods, and Aglantha) from 1964 to 1967, species identifications, counts and lengths for many samples collected between 1968 to 1980. The attached supporting document (Ocean Station "Papa" detailed zooplankton data: 1956 – 1980) contains information on the methods used to collect and process the data along with descriptions of a number of fairly minor points about the data that were not resolved. It also describes, in detail, the format of the original data files, the corrections/changes that were made to these files in creating this version, and how these errors affect what was published in Fulton (1983).The purpose of this record is to make the detailed data available to the scientific community in an electronic format and to provide a convenient reference for citing the detailed data. Waddell, Brenda J., and Skip McKinnell. 1995. Ocean Station "Papa" detailed zooplankton data:1956 - 1980. Can. Tech. Rep. Fish. Aquat. Sci. 2056: 21 p.