A research survey of scallops (mainly sea scallop Placopecten magellanicus, but also Icelandic Scallop Chlamys islandica) using a dredge was carried out by DFO (Fisheries and Oceans Canada) every 1 or 2 years since 1992 in the Magdalen Islands (fishing area 20). The main objective of this research survey was to assess Sea Scallop stocks. Another objective was to document taxa associated with scallop habitat according to a fixed random sampling plan. Occurrences for the year 2021 and 2022 are presented by species (or taxon) by station. Starting in 2021, catches were weighed, and specimens photographed, with information available upon request. The taxonomic and geographical validity of the data was checked and the World Register of Marine Species served as the taxonomic authority for naming all taxa recorded during the survey. Epibenthic invertebrates (mainly molluscs, echinoderms and crustaceans) as well as demersal fish were identified from the dredge catches. The historical dataset is available at the following link : https://open.canada.ca/data/en/dataset/71732ad5-5c70-4dbf-916d-a94e1380c53bThe study area is located south of the Magdalen Islands and the sampling of scallop beds is carried out at depths of generally around 25 to 35 m. A random selection of sampling stations is carried out from a fixed station grid. Sampling is done along transects at these randomly drawn stations in the study area. Sampling is done with a lined Digby scallop dredge (20 mm mesh) over approximately 500 m along the seabed. The four baskets of the dredge are examined for all scallops, and starting in 2022, also for all fishes. One basket (first on the starboard side) is sorted and examined for associated species. Most specimens are counted by taxa. Those that are too small and numerous, or colonial, are noted for presence or relative abundance. Special cases are occasionally conserved for taxonomic analysis, for example, ascidians (to monitor for invasive species) and sponges (to document new species). The availability of photos and some conserved specimens enables future review. Changes are anticipated in the identifications, notably for Bryozoan, Hydrozoa, and Porifera, that are currently the focus of research efforts.

A research survey of scallops (mainly sea scallop Placopecten magellanicus, but also Icelandic Scallop Chlamys islandica) using a dredge was carried out by DFO (Fisheries and Oceans Canada) every 1 or 2 years since 1992 in the Magdalen Islands (fishing area 20). The main objective of this research survey was to assess Sea Scallop stocks. Another objective was to document taxa associated with scallop habitat according to a fixed random sampling plan. Occurrences by species (or taxon) are presented by station. The taxonomic and geographical validity of the data was checked and the World Register of Marine Species served as the taxonomic authority for naming all taxa recorded during the survey. Epibenthic invertebrates (mainly molluscs, echinoderms and crustaceans) as well as demersal fish were identified from the dredge catches. The current data starting in 2021 are available at the following link : https://open.canada.ca/data/en/dataset/6529a4b0-f863-4568-ac71-1fa26cf68679The study area is located south of the Magdalen Islands and the sampling of scallop beds is carried out at depths of 10 to 38 m, generally around 25 to 35 m. A random selection of sampling stations is carried out from a fixed station grid. Sampling is done along transects at these randomly drawn stations in the study area. Sampling is done with a lined Digby scallop dredge (20 mm mesh) over approximately 500 m along the seabed. The four baskets of the dredge are examined for all scallops. Next, a basket (the first on the starboard side) is sorted and examined for associated species. Most specimens are counted by taxon. The presence or relative abundance of undersized and numerous, or colonial, organisms is noted. Special cases are sometimes retained for taxonomic analysis, for example, ascidians (to monitor invasive species) and sponges (to document new species).

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

The porbeagle shark (Lamna nasus), is a species found in Atlantic Canadian waters which is encountered in commercial and recreational fisheries. Pop-up Satellite Archival Tags (PSAT) from Wildlife Computers were applied to porbeagle sharks from 2005 to 2021 to collect data on depth (pressure), temperature and ambient light level (for position estimation). Deployments were conducted in Canada and the Faroe Islands on commercial, recreational and scientific charters, typically in summer and fall but some over winter when the porbeagle commercial fishery was active in Canada. A variety of tag models were deployed: PAT 4 (n=1), Mk10 (N=41), and MiniPAT (N=15) and 51 of 57 tags reported. One individual shark was recaptured and the physical tag was returned. The porbeagle sharks tagged ranged in size from 76 cm to 249 cm Fork Length (curved); 42 were female, 15 were male. Time at liberty ranged from 4 – 356 days and 14 tags remained on for the programmed duration. Raw data transmitted from the PSAT’s after release was processed through Wildlife Computers software (GPE3) to get summary files, assuming a maximum swimming speed of 2m/s, NOAA OI SST V2 High Resolution data set for SST reference and ETOPO1-Bedrock dataset for bathymetry reference. The maximum likelihood position estimates are available in .csv and .kmz format and depth and temperature profiles are also in .csv format. Other tag outputs as well as metadata from the deployments can be obtained upon request from: warren.joyce@dfo-mpo.gc.ca or heather.bowlby@dfo-mpo.gc.ca.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

11 tagged Blue whales (Balaenoptera musculus) were tracked during the daytime movements as well as the feeding behaviour in the St. Lawrence River estuary. Kernel density was applied to derminate the high density feeding areas of all individuals combined (30, 40, 50, 60, 75, 95 %).Doniol-Valcroze T, Lesage V, Giard J, Michaud R, 2012. Challenges in marine mammal habitat modelling: evidence of multiple foraging habitats from the identification of feeding events in blue whales. Endang Species Res, Vol. 17 : 255–268, doi : 10.3354/esr00427(English version only)

Speckled Dace (Rhinichthys osculus) are listed under the Species at Risk Act (SARA) as endangered. These fresh water fish, of the family Cyprinidae, are found in Canada only in the Kettle Valley of British Columbia. Proposed critical habitat was based on minimum viable population analysis and assumed densities of fish. From October 19th to the 22nd of 2015, night time pole seining surveys were conducted to enumerate Speckled Dace in proposed critical habitat on the West Kettle River; one of three rivers containing Speckled Dace. The estimated population abundance of Speckled Dace within the survey area was 8,978 (6,143-11,814), however only 1,014 of these are estimated to be adults.

The mako shark (Isurus oxyrinchus), is a species found in Atlantic Canadian waters which is encountered in commercial and recreational fisheries. Pop-up Satellite Archival Tags (PSAT) from Wildlife Computers were applied to mako sharks from 2011 to 2013 to collect data on depth (pressure), temperature and ambient light level (for position estimation). Deployments were conducted in Canada on commercial vessels, typically in summer and fall from July to October. Two types of tag models were deployed: Mk10 (N=28), and MiniPAT (N=9) and 28 of 37 tags reported (one female shark was recaptured). The mako sharks tagged ranged in size from 80 cm to 229 cm Fork Length (curved); 13 were female, 17 were male, and 7 were unknown sex. Time at liberty ranged from 0 – 185 days and 6 tags remained on for the programmed duration. Raw data transmitted from the PSAT’s after release was processed through Wildlife Computers software (GPE3) to get summary files, assuming a maximum swimming speed of 2m/s, NOAA OI SST V2 High Resolution data set for SST reference and ETOPO1-Bedrock dataset for bathymetry reference. The maximum likelihood position estimates are available in .csv and .kmz format and depth and temperature profiles are also in .csv format. Other tag outputs as well as metadata from the deployments can be obtained upon request from: warren.joyce@dfo-mpo.gc.ca or heather.bowlby@dfo-mpo.gc.ca.

"Fall" missions occur primarily in October and November, but sets from September and December are also present in the data. Collected data includes total catch in numbers and weights by species. Length frequency data is available for most species, as are the age, sex, maturity and weight information for a subset of the individual animals. Other data such as ageing material, genetic material, and stomach contents are often also collected, but are stored elsewhere."Fall" cruises occur in September, October, November and December.Cite this data as: Clark, D., Emberley, J. Data of Maritimes Fall Research Vessel Survey. Published January 2021. Population Ecology Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/5f82b379-c1e5-4a02-b825-f34fc645a529

PURPOSE:As a part of a two-decade series of research, this study aims to provide a comprehensive synthesis of the effects of harvest and environmental change on fisheries in Great Bear Lake. The main objectives are to assess demographic traits and the current status of harvested species, with a focus on evaluating sustainable harvest levels of lake trout, a cold-adapted species with a relatively narrow thermal niche. As part of this research, trends in water quality and primary productivity are monitored to evaluate potential effects of change on fisheries. DESCRIPTION:Great Bear Lake, one of the largest lakes in North America, contains culturally and recreationally important fish species. Great Bear Lake is located in the sub-Arctic and Arctic Circle. As part of a two-decade series of research aimed to provide a comprehensive synthesis of the effects of harvest and environmental change on fisheries in Great Bear Lake, the main objectives of this study are to assess demographic traits and the current status of harvested species, with a focus on evaluating sustainable harvest levels of lake trout, a cold-adapted species with a relatively narrow thermal niche. As part of this research, trends in water quality and primary productivity are monitored to evaluate potential effects of change on fisheries. From 2012 to 2019, surface water temperature data was collected at depths of 0.1 to 1.0 meters using a Hydrolab Series 5 Data Sonde Multiparameter instrument through partnered community-led and community/Fisheries and Oceans Canada/university partners collaborative sampling. The project has strong community involvement, including youth through the Guardian Program, to facilitate capacity building and community leadership in the long-term monitoring of Great Bear Lake fisheries and the aquatic ecosystem. This data is an extension of baseline data sets on water quality on the lake. These data will contribute to a better understanding cumulative impacts of climate change on the functioning of large northern lake ecosystems and provide a benchmark for monitoring further change. This data will be important for developing effective strategies for maintaining community-led aquatic monitoring and managing natural resources, particularly fish, which are expected to be increasingly important to communities with declines in other country foods such as caribou. We acknowledge the data were collected in the Sahtú Settlement Area and are made publicly available with the agreement of the Délı̨nę Renewable Resources Council (Délı̨nę Ɂehdzo Got’ı̨nę (Renewable Resources Council)).Collaborators include: the Community of Délı̨nę partners (data collection), Délı̨nę Renewable Resource Council, University of Manitoba, University of Queens, University of British Columbia, University of Alberta, Environment and Climate Change Canada, and Great Lakes Fisheries Commission, Sahtú Renewable Resource Board. Community of Délı̨nę partners and field workers that participated in data collection include Daniel Baton, Morris Betsidea, Joey Dillion, Jade English, Stanley Ferdanan, Bruce Kenny, Elaine Kenny, Darren Kenny, Greg Kenny, Joseph Kenny, Rocky Kenny, Ted Mackienzo, George Menacho, Bobby Modeste, Gina Nyelle, Brent Taniton, Allison Tatti, Gerald Tutcho, Archie Vital, Barbara Yukon, Caroline Yukon, Chris Yukon, and Cyre Yukon.Funding and logistical support was provided by: Northwest Territories Cumulative Impact Monitoring, Natural Sciences and Engineering Research Council of Canada, Environment and Climate Change Canada, Sahtú Renewable Resource Board, the Polar Continental Shelf Program, and Fisheries and Oceans Canada.