Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Nekton assemblages in Zostera marina beds and adjacent bare soft-sediments were sampled on the south and eastern shore of Nova Scotia. Sampling gear used were visual snorkel transects and a benthic beam trawl. Fish were identified and size either measured (trawl) or estimated in situ (snorkel transects). Surveys were conducted in mid-July to August in summer of 2013 and 2014 across multiple sampling sites. Multiple replicate transects were conducted at each site. Raw abundances from observations were transformed into young of year (YOY) equivalent abundance, and then into density of each species calibrated to account for the sampling equipment and day/night differences.Cite this data as: Wong, M. C. Data of: Fish and large decapods in eelgrass (Zostera marina) beds on the Atlantic coast of Nova Scotia, Canada. Published: April 2020. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/dbc56f11-4a97-45e7-99f4-71966b51630c

Harbour seals (Phoca vitulina) are found along temperate and Arctic marine coastlines of the Northern Hemisphere. They are found in coastal waters of the northern Atlantic and Pacific Oceans, as well as those of the Baltic and North Seas. In Canada, they may be found off the coastal waters of British Columbia, Nunavut, Manitoba, Ontario, Quebec, New Brunswick, Prince Edward Island, Nova Scotia, and Newfoundland and Labrador.Population trends and abundance of harbour seals in British Columbia are assessed based on aerial surveys conducted during 1966-2019. Based on counts conducted in Index Areas distributed throughout the province, the trend observed in the Strait of Georgia appears to be generally indicative of harbour seal populations throughout British Columbia. Total abundance of harbour seals on the B.C. coast in 2008 was estimated to be on the order of about 105,000 (95% confidence interval of 90,900 to 118,900) seals. Total abundance was re-estimated in 2022 (estimate and CI pending completion of CSAS process). Historic reconstructions indicate the population was depleted by a period of commercial harvesting during 1879-1914, and subsequently maintained below natural levels by predator control programs until the early 1960s. Already depleted, the population could not sustain a second period of intense commercial harvesting during 1962-1968 and was further depleted, but now appears to have fully recovered.

This project was completed by the Productive Capacity group (Coastal and Freshwater Ecology Section) in the Newfoundland and Labrador Science Branch of Fisheries and Oceans Canada (DFO). American lobster (Homarus americanus) is a commercially important decapod crustacean species along the east coast of North America, ranging from the Labrador coast south to Cape Hatteras. Juvenile lobster < 40 mm CL (carapace length) recruitment has been studied extensively in the southern portions of their range. However, investigations of settlement habitat association and recruitment of juvenile lobster in the northern extremes along the Newfoundland coast have been largely unsuccessful. We investigated juvenile lobster density, habitat, and depth associations in three areas of Newfoundland, using scuba – Port Saunders area, 8 dives conducted 28 September - 2 October 2017, and Burin Peninsula 10 dives (7 Placentia Bay, 3 Fortune Bay) conducted 30 September - 4 October 2018 over a total seabed area of 9,138 m2, within 200 meters of shore. Port Saunders and Fortune Bay had relatively higher lobster density (0.09 and 0.40 m-2, respectively); >65% were juveniles, in contrast to Placentia Bay where lobster densities of all size groups were low (mean 0.01 m-2) and no juvenile lobsters were observed at all. Where observed all juvenile lobster were significantly associated with shallow (<6 m) habitat and showed no overlap with distribution of adults (>82.5 mm CL) which we observed at depths 6 to 17 m. Our sites were dominated by varying mixes of cobble and pebble (77%); rock/bedrock (12%) and mud/sand/small pebble (11%) substrates interspersed with overlying kelp (32%) and eelgrass (11%) vegetation. We observed no significant associations with substrate or vegetation. This record contains the geographic locations of the 7 Placentia Bay sites surveyed, and information on the timing and type of data collected at each site, which was one component supported by Coastal Environmental Baseline Program of a larger collaborative project.

Historical finds of Coleophora serratella

Monitoring of Unit 2 redfish by Fisheries and Oceans Canada (DFO) ceased in 2002. Since then, the Atlantic Groundfish Council (AGC, formerly the Groundfish Enterprise Allocation Council [GEAC]) has funded surveys approximately every two years in the area, in collaboration with DFO. Over the years, various vessels and gear types have been used. In 2024, a comparative survey was conducted using the CCGS John Cabot and a fishing industry vessel (the Léry Charles) to develop conversion factors that allow data from the 2020, 2022, and 2024 Unit 2 redfish surveys to be standardized to CCGS John Cabot equivalents. The survey covered the waters off southwestern Newfoundland and eastern Cape Breton, corresponding to redfish management Unit 2, which includes NAFO subdivisions 3Pn, 3Ps, 4Vn, 4Vs, and 4W. Here, data collected aboard the CCGS John Cabot are presented. Objectives• Assess the abundance and condition of groundfish and invertebrates• Assess environmental conditions• Inventory the biodiversity of benthic and demersal megafauna• Monitor the pelagic ecosystem• Collect samples for various research projectsData The CCGS John Cabot employed a four-sided modified Campelen 1800 shrimp trawl, fitted with a Rockhopper ("bicycle") footgear. The trawl extension and codend were lined with 12.7 mm knotless nylon mesh. Standard trawl tows were 15 minutes in duration, timed from bottom contact, with a target towing speed of 3 knots.For each fishing tow, the catch is sorted and weighed by taxa; individuals are then counted and biological data are collected on a subsample. For fish, crab and squid, size and weight are measured by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish, and the otoliths are sampled for several species such as Atlantic cod, Atlantic halibut, and Greenland halibut. Roughly 2-kg of shrimp are sorted and weighed by species. The other invertebrates are counted (no individual measurements) and photographed.The biological data are divided into 3 files: a “Stations” file containing set information, a “Catches” file containing catches per set for all species caught and a “Carbio” file containing biological and morphometric measurements per individual all species measured except shrimps. The columns source_info, no_survey, nbpc and station_number serve as a common key linking the three datasets. To further link the “Captures” and the “Carbio” files to the species level (for fish and certain invertebrate species), use the columns source_info, no_survey, nbpc, station_number, strap_code, and category. Further shrimp biological and morphometric data are available but are not presented here in order to simplify data presentation. For access to the complete information, please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca). The latest published data is preliminary and will be updated at the end of January of the following year.It is important to note that this is raw data. Only sets considered successful are retained. In each set, all species are kept, with a few exceptions. Data is available from 1997-2022 but please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca) for access and further details. For any other information please also contact the data management team.

AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas.Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador.Reference:TunicatesViolet tunicate (Botrylloides violaceus) 2007The violet tunicate was first detected in NL waters in 2007 in Belleoram, Fortune Bay on wharf structures and vessels (McKenzie et al. 2016b). This colonial tunicate forms irregular shaped colonies usually of a solid color (orange, purple, yellow or cream). It is currently found in relatively small colonies in four harbours in NL; Placentia Bay (1), Fortune Bay (1), Conception Bay (1) and the west coast of NL (2). The data provided here indicates the detections of this AIS in coastal NL.From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

Historical finds of Coleophora laricella

Fisheries and Oceans Canada (DFO) conducts an annual summer multidisciplinary scientific survey with a bottom trawl in the Estuary and the northern Gulf of St. Lawrence since 1984. Over the years, this survey has been conducted on four vessels: the MV Lady Hammond (1984-1990), the CCGS Alfred Needler (1990-2005), the CCGS Teleost (2004-2021) and the CCGS Cabot (2022-current). It is important to note that the objectives, the methods used and the identification of the species during these surveys have improved over time in response to DFO requests and mandates. The data are therefore not directly comparable between these surveys. The specificities of the missions onboard the MV Lady Hammond are described below.Objectives:1. Assess groundfish populations abundance and condition2. Assess environmental conditions3. Conduct a biodiversity inventory of benthic and demersal megafauna4. Monitor the pelagic ecosystem5. Collect samples for various research projectsSurvey descriptionThe survey covers the northern Gulf of St. Lawrence, that is the divisions 4R, 4S and the northern part of division 4T of the Northwest Atlantic Fisheries Organization (NAFO). A stratified random sampling strategy is used for this survey and the fishing gear used on the MV Lady Hammond is a bottom trawl Western IIA. Standard trawling tows last 30 minutes, starting from the time the trawl touches the sea floor. Towing speed is 3.5 knots.DataFor each fishing tow, the catch is sorted and weighed by taxa; individuals are counted and biological data are collected on a sub-sample. For fish, crab and squid, size and weight are measured by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish and otoliths are collected for redfish and Atlantic cod. Invertebrates are weighted and counted (no individual measurements).The biological data are divided into 4 files: a “Metadata” file containing set information, a “Catches” file containing catches per set for fish taxa, a “Carbio” file containing biological and morphometric measurements per individual and a “Freql” file containing the length frequency of fish. It's important to note that this is raw data. Only sets considered successful are retained. In each set, all species are kept, with a few exceptions. For more information please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca).

The layer presents the information on the distribution of eelgrass (Zostera marina) beds in James Bay, Chaleur Bay, Estuary and Gulf of St. Lawrence according to a literature review of documents produced between 1987 and 2009. Additional InformationEelgrass's inventory was produced according to a literature review of the following documents:Calderón, I. 1996. Caractérisation de la végétation et de la faune ichtyenne de la baie de Sept-Îles. Document réalisé par la Corporation de protection de l'environnement de Sept-Îles pour Pêches et Océans Canada. 26p. + 5 annexes.Comité côtier Les Escoumins à la Rivière Betsiamites. 2004. Inventaire de localisation des bancs de zostère marine dans la zone côtière Les Escoumins à la rivière Betsiamites. 9 p.Comité ZIP Côte-Nord du Golfe. 2001. Inventaire du potentiel côtier et marin de la Basse-Côte-Nord. Version préliminaire de rapport sous forme de CD-ROM, Sept-Îles, mars 2001.Comité ZIP de la rive nord de l’estuaire. 2008. Guide d’intervention en matière de protection et de mise en valeur des habitats littoraux d’intérêt de la rive nord de l’estuaire maritime (fiches 14 à 20). 8 p. + 7 fiches + annexe.Conseil Régional de l’Environnement Gaspésie et des Îles-de-la-Madeleine (2004). Inventaire et étude des bancs de zostère marine sur le territoire couvert par les comités de gestion intégrée de la zone côtière de l’Est du Québec. CONSORTIUM GAUTHIER & GUILLEMETTE - G.R.E.B.E. 1992. Description et cartographie des habitats côtiers de la Baie de Hannah jusqu'à la rivière au Castor. Rapport présenté à Hydro-Québec, Complexe Nottaway-Broadback-Rupert (NBR), Vol. 2, Annexe cartographique.Giguère, M., C. Duluc, S. Brulotte, F. Hazel, S. Pereira et M. Gaudet. 2006. Inventaire d’une population d'huître américaine (Crassostrea virginica) dans le Bassin aux Huîtres aux Îles-de-la-Madeleine en 2005. Rapport manuscrit. vi + 21 p.Grant, C. et L. Provencher, 2007. Caractérisation de l’habitat et de la faune des herbiers de Zostera marina (L.) de la péninsule de Manicouagan (Québec). Rapp. tech. can. sci. halieut. aquat. 2772 : viii + 65 p. Groupe Environnement Littoral. 1992. Complexe NBR. La zostère marine. Rapport présenté à la vice-présidence Environnement d'Hydro-Québec. 9 p. + 2 cartes.Harvey, C. et D. Brouard. 1992. Étude exploratoire du barachois de Chandler: aspects biophysiques et contamination. Rapport présenté à Environnement Canada, Direction de la protection de l'environnement région du Québec. 39 p. et annexes.Hazel, François, 2002. Données de terrain prises par F. Hazel, Septembre 2002.Ellefsen, H.-F. 2009. Communication personnelle de Hans-Frédéric Ellefsen (MPO).Jacquaz et coll. 1990. Étude biophysique de l'habitat du poisson de quatre barachois de la baie des Chaleurs.Kedney, G. et P. Kaltenback. 1996. Acquisition de connaissances et mise en valeur des habitats du banc de Portneuf. Document réalisé par la firme Pro Faune pour le Comité touristique de Rivière-Portneuf. 50 pages et 5 annexes.Lalumière, R. 1987. Répartition de la zostère marine (Zostera marina) sur la côte est de la baie James; été 1987. Rapport produit par Gilles Shooner et Associés inc. pour la Société d’énergie de la Baie James. 30 p. et annexes.Lalumière, R., L. Belzile et C. Lemieux. 1992. Étude de la zostère marine le long de la côte nord-est de la baie James (été 1991). Rapport présenté au Service écologie de la SEBJ. 31 p. + carte.Leblanc, J. 2002. Communication personnelle de Judith Leblanc (MPO).Lemieux, C. 1995. Acquisition de connaissances des habitats côtiers dans la région de Rimouski (1995). Rapport du Groupe-Conseil GENIVAR présenté au Ministère des Pêches et des Océans du Canada, Division de la Gestion de l’Habitat du Poisson, 52 pages + 2 annexes.Lemieux, C. et R. Lalumière. 1995. Acquisition de connaissances des habitats côtiers du barachois de Saint-Omer. Rap. du Groupe conseil Genivar inc. pour la DGHP, MPO, 44 pages + 3 ann.Martel, Marie-Claude, Lizon Provencher, Cindy Grant, Hans-Frédéric Ellefsen et Selma Pereira, 2009. Distribution and description of eelgrassbeds in Québec. Fisheries and Oceans Canada, Canadian Science Advisory Secretariat, Research Document 2009/050. 45p. Morin, D. 2009. Communication personnelle de Danièle Morin (MRNF).Naturam Environnement. 1999. Caractérisation biophysique, socio-économique et détermination des enjeux dans un secteur potentiel pour l’identification d’une zone de protection marine pilote: portion ouest de la MRC Manicouagan. Baie-Comeau. 311 p. Pelletier, Claudel. 2003. Communication personnelle de Claudel Pelletier, FAPAQ, lettre en date du 24 février 2003.Pereira, S. 2009. Communication personnelle de Selma Pereira (MPO).Vaillancourt, M.-A. et C. Lafontaine. 1999. Caractérisation de la Baie Mitis. Jardins de Métis et Pêches et Océans Canada. Grand-Métis. 185 p.