This inventory, conducted from September 26th to October 3th, 2019, aimed to describe the community structure of macroalgae and benthic macroinvertebrates of five small estuaries of the Upper North Shore of Quebec, namely Barthélemy Bay and the Colombier, Mistassini, Franquelin and Saint-Nicolas rivers. This inventory is part of a doctoral study of Valentine Loiseau on the global changes in the St. Lawrence system, mainly the study of marine benthic communities in response to changes of salinity, to ensure proper management of the environment in the face of future changes. The main objective is to describe the structure and the levels of specific diversities of mediolittoral communities of benthic macroinvertebrates and macroalgae along a salinity gradient. These five small estuaries were selected because of their similar size, hard substrates and easy access. Three levels of hypoosmotic stress (low, medium, high) and one control level (seawater) were used for each of the selected estuaries, with eight quadrats per stress level. Quadrat positions were randomly selected but had to meet two criteria: (1) regular height in the foreshore to control the influence of other stresses (temperature, exposure); and (2) presence of at least one macroalga to maintain homogeneity. A percentage cover by macroalgal and macroinvertebrate species was estimated, and then all organisms were weighed by species and size group. The salinity of the nearest water point was measured at mid-tide with a portable refractometer and a Castaway-type CTD (Conductivity-Temperature-Density) probe. The inventory was done using a stratified random sampling design and the sampling unit was a quadrat measuring 25 x 25 cm. The three files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "event_information" file includes the generic information of the quadrat, including date and location. The "additional_information_event_and_occurrence" file includes salinity and substrate type of the quadrat, as well as the total weight of all individuals of the same species caught in the quadrat extrapolated to one square metre of surface. For nudibranchs and barnacles, weight was estimated from the size of the individuals so that they were not removed from the environment. The "taxon_occurrence" file includes the taxonomic inventory of macroalgal and benthic macroinvertebrate species observed in the quadrat, identified to the lowest possible species or taxonomic level and biomass by identified species.For quality control, organisms were identified on the field using the following guide: Chabot, Robert et Anne Rossignol. 2003. Algues et faune du littoral du Saint-Laurent maritime : Guide d'identification. Institut des Sciences de la mer de Rimouski, Rimouski ; Pêches et Océans Canada (Institut Maurice-Lamontagne), Mont-Joli. 113 pages. The taxonomy was checked against the World Register of Marine Species (WoRMS) to match recognized standards and using the R obistools and worrms libraries. The WoRMS match was placed in the "scientificNameID" field of the occurrence file. All sample locations were spatially validated. This project was funded by DFO Coastal Environmental Baseline Program under Canada’s Oceans Protection Plan. This initiative aims to acquire environmental baseline data contributing to the characterization of important coastal areas and to support evidence-based assessments and management decisions for preserving marine ecosystems.

Seamounts have been identified as Ecologically or Biologically Significant Areas (EBSAs) due to their unique oceanography and ecology; they frequently serve as sites for fisheries and as habitat for a number of species of conservation concern. A mix of isolated seamounts and seamount complexes are distributed throughout Canada’s Pacific offshore waters, although only a subset of these are named. We used several pre-existing spatial databases and predictive models to map all named seamounts within Canada’s Exclusive Economic Zone (EEZ), all named seamounts fished by Canada in international waters, and any predicted (modelled) unnamed seamounts in the EEZ. These data are intended to inform marine planning initiatives in BC by providing collaborative, peer-reviewed scientific data at scales relevant to a BC coast-wide analysis.

The Department of Fisheries and Oceans (DFO) Science Branch has designed a multispecies dive survey protocol to provide unbiased, coast wide monitoring of benthic invertebrate stocks (as may be required under the updated Fisheries Act) and associated habitat information for a suite of benthic marine invertebrate species (Green (Strongylocentrotus droebachiensis), Purple (S. purpuratus) and Red Sea Urchin (Mesocentrotus franciscanus), Geoduck (Panopea generosa), Giant Red Sea Cucumber (Apostichopus californicus), Northern Abalone (Haliotis kamtschatkana), and Sunflower Sea Star (Pycnopodia helianthoides)). Based on information available at this time, the proposed survey design can provide estimates of coast wide stock status for Red Sea Urchin and Giant Red Sea Cucumber, and relative abundance indices for Geoduck, Green Sea Urchin, Purple Sea Urchin, Northern Abalone and Sunflower Sea Star.The new protocol was tested through a series of pilot surveys conducted on a subset of areas of the BC coast each September from 2016-2021. Design of the pilot surveys was based on previous dive survey data and experience and demonstrated the practical feasibility of the protocol, while also gathering preliminary information to guide recommendations about the statistical design of the survey.The dataset consists of a relational database containing tables representing each component of the survey methodology. The primary component of the survey is a transect location. Along each transect, systematically spaced quadrats are sampled, and on each quadrat, substrate observations are recorded, multiple species of algae are recorded, and multiple individual invertebrates are measured or counted. The tables are linked by transect number and quadrat number.

PURPOSE:The Department of Fisheries and Oceans (DFO) conducted a baseline survey of biological communities and habitat parameters in the offshore Canadian Beaufort Sea between 2012 and 2014, as part of the federally administered Beaufort Regional Environmental Assessment. The BREA-Marine Fishes Project (BREA-MFP) was the first comprehensive baseline study of offshore marine fish diversity and associated habitats in the Canadian Beaufort Sea. Knowledge gained during the BREA-MFP supports regulatory processes pertaining to offshore development and Oceans Management in the Inuvialuit Settlement Region, and provides baseline context for studies of the effects of climate change and variability. The Canadian Beaufort Sea – Marine Ecosystem Assessment (CBS-MEA, 2017-2019 and 2021-2024) is building on system baselines and ecological knowledge derived from the BREA-MFP to develop a comprehensive research and monitoring approach for the offshore Canadian Beaufort Sea. This approach will enable us to better understand the relationship between oceanographic drivers and ecosystem responses. The CBS-MEA focuses on integrating oceanography, food web linkages, physical-biological couplings and spatial and inter-annual variabilities, while also expanding baseline coverage of species diversity, abundances, and habitat associations to areas of the Beaufort Sea and Canadian Archipelago that are previously unstudied in this context.DESCRIPTION:Between 2017 and 2019, and between 2021 and 2024, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope in August and early September each year. Sampling was conducted from the F/V Frosti at over 150 stations along ten multi-year transects, and over 50 non-transect stations. Standardized sampling was conducted at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

This dataset contains the abundance (per m²) and the biomass (mg dry per m²) of macrofauna (≥ 500µm) in eelgrass and adjacent bare soft sediments, collected at sites in the Atlantic of Nova Scotia from 2009 to 2013.Cite this data as: Wong M.C. Data of Benthic invertebrates in seagrass and bare soft sediments in Atlantic Nova Scotia Published May 2020. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/05d5f46a-7f19-11ea-8a4e-1860247f53e3Publications: Wong, M. C., & Dowd, M. (2021). Functional trait complementarity and dominance both determine benthic secondary production in temperate seagrass beds. Ecosphere. 12(11), e03794. https://doi.org/10.1002/ecs2.3794Wong, M. C. (2018). Secondary Production of Macrobenthic Communities in Seagrass (Zostera marina, Eelgrass) Beds and Bare Soft Sediments Across Differing Environmental Conditions in Atlantic Canada. Estuaries and Coasts, 41, 536–548. https://doi.org/10.1007/s12237-017-0286-2

The Brier Island/Digby Neck area has been identified as an Ecologically and Biologically Significant Area (EBSA) by Fisheries and Oceans Canada and is one of four marine areas within the Bay of Fundy recognised by Parks Canada as of national significance for marine conservation planning. The area is representative of important outer Bay of Fundy features with significant marine mammal, bird, and benthic diversity including potentially important aggregations of sensitive benthic species such as horse mussel and sponge. Much of the information used for this recognition is now over 40 years old and should be re-validated using standardised georeferenced survey methods. As a first phase, a diver-based survey of the sublittoral habitats and associated species was conducted in August and September of 2017 for the Brier Island area. This report summarises the major sublittoral habitat types, species assemblages, and oceanographic conditions observed at 20 locations including Northwest and Southwest Ledges, Gull Rock, Peter’s Island, and Grand Passage. A total of 962 records were made of 178 taxa, consisting of 43 algae and 135 animals. Comparison with historical records largely confirmed the continued presence of unique habitats and species assemblages for which this area was initially recognised as an EBSA. Differences in species richness observed for cryptic and less known taxonomic groups such as sponges and bryozoans were attributable to changes in survey methods and knowledge. Based on these findings, additional surveys of inshore and offshore Brier Island using more quantitative methods developed for other Bay of Fundy EBSAs would further support regional MPA network planning and provide relative scales of species diversity and habitat coverage for this area.

PURPOSE:In this study, we examined the structure and function of the Southampton Island marine food web across 149 species of benthic and pelagic invertebrates, fishes, marine mammals and seabirds collected from 2016 to 2019, to provide a baseline for future studies that aim to quantify temporal changes in food web structuring. More specifically,we used a multi-biomarker approach combining stable isotopes and HBIs to: (i) determine the vertical trophic structure of the marine food web, (ii) investigate the contribution of benthic and pelagic-derived prey to the higher trophic level species of the Arctic food web, and (iii) determine the role of ice algae and phytoplankton carbon source use across different trophic levels and compartments (pelagic and benthic). By shedding new light on the functioning of the Southampton Island food web and specifically how the contribution of ice algae and benthic habitat shapes its structure, these results will be relevant to adaptive management and conservation initiatives implemented in response to anthropogenic stressors and climate change. DESCRIPTION:Climate-driven alterations of the marine environment are most rapid in Arctic and subarctic regions, including Hudson Bay in northern Canada, where declining sea ice, warming surface waters and ocean acidification are occurring at alarming rates. These changes are altering primary production patterns that will ultimately cascade up through the food web. Here, we investigated (i) the vertical trophic structure of the Southampton Island marine ecosystem in northern Hudson Bay, (ii) the contribution of benthic and pelagic-derived prey to the higher trophic level species, and (iii) the relative contribution of ice algae and phytoplankton derived carbon in sustaining this ecosystem. For this purpose, we measured bulk stable carbon, nitrogen and sulfur isotope ratios as well as highly branched isoprenoids in samples belonging to 149 taxa, including invertebrates, fishes, seabirds and marine mammals. We found that the benthic invertebrates occupied 4 trophic levels and that the overall trophic system went up to an average trophic position of 4.8. The average δ34S signature of pelagic organisms indicated that they exploit both benthic and pelagic food sources, suggesting there are many interconnections between these compartments in this coastal area. The relatively high sympagic carbon dependence of Arctic marine mammals (53.3 ± 22.2 %) through their consumption of benthic invertebrate prey, confirms the important role of the benthic subweb for sustaining higher trophic level consumers in the coastal pelagic environment. Therefore, a potential decrease in the productivity of ice algae could lead to a profound alteration of the benthic food web and a cascading effect on this Arctic ecosystem.Collaborators:Centre for Earth Observation Science, University of Manitoba, Winnipeg, Manitoba, Canada - R´emi Amiraux, C.J. Mundy, Jens K. Ehn, Z.A. Kuzyk.Quebec-Ocean, Sentinel North and Takuvik, Biology Department, Laval University, Quebec, Quebec, Canada - Marie Pierrejean.Scottish Association for Marine Science, Oban, UK - Thomas A. Brown.Department of Natural Resource Sciences, McGill University, Ste. Anne de Bellevue, Quebec, Canada - Kyle H. Elliott.Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada - Steven H. Ferguson, Cory J.D. Matthews, Cortney A. Watt, David J. Yurkowski.School of the Environment, University of Windsor, Windsor, Ontario, Canada - Aaron T. Fisk.Science and Technology Branch, Environment and Climate Change Canada, Ottawa, Ontario, Canada - Grant Gilchrist.College of Fisheries and Ocean Sciences, University of Alaska Fairbanks, Fairbanks, AK, USA - Katrin Iken.Department of Earth Sciences, University of New Brunswick, Fredericton, NB, Canada - Audrey Limoges.Department of Integrative Biology, University of Windsor, Windsor, Ontario, Canada - Oliver P. Love, Wesley R. Ogloff.Department of Arctic Biology, The University Centre in Svalbard, Longyearbyen, Norway - Janne E. Søreide.

In 2012 and 2013, Fisheries and Oceans Canada surveyed the benthos in two areas closed to bottom contact fishing, the Narwhal Overwintering and Coldwater Coral Zone (now the Disko Fan Conservation Area, DFCA), and the Hatton Basin Voluntary Coral Protection Zone (now the Hatton Basin Conservation Area, HBCA). Samples were collected following protocols recommended by the Arctic Council’s Circumpolar Biodiversity Monitoring Plan for the purposes of providing baseline data for future monitoring of benthic invertebrates in this sensitive region, and for facilitating pan-Arctic comparisons of benthic communities. Five biodiversity monitoring stations were established, four in the DFCA and one in the HBCA, each of which was fully sampled according to those protocols with Van Veen grabs or box corers, drop cameras and temperature recorders attached to the gear. This report summarises the grab/core-sampled benthic fauna collected during the 2012 survey of the Conservation Areas and complements another report documenting the epibenthos from the camera transects in the DFCA. Here we report on macrofauna in the 1-cm size fraction, and on foraminiferan meiofauna.The data provided is presented in the following report (see related link) :Jacobs, K., Bouchard Marmen, M., Rincón, B., MacDonald, B., Lirette, C., Gibb, O., Treble, M., and Kenchington, E. 2022. Biodiversity Monitoring Stations for Benthic Macrofauna and Meiofauna in the Disko Fan and Hatton Basin Conservation Areas. Can. Tech. Rep. Fish. Aquat. Sci. 3487: vi + 86 p.Cite this data as: Bouchard Marmen, Marieve; Rincon, Beatriz ; MacDonald, Barry; Lirette, Camille; Gibb, Olivia; Treble, Margaret ; Jacobs, Kevin; Kenchington, Ellen (2022). Biodiversity Monitoring Stations for Benthic Macrofauna and Meiofauna in the Disko Fan and Hatton Basin Conservation Areas. Published January 2023. Ocean Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/b7bcff18-698b-4d40-a7bd-13d39925cbeb

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

PURPOSE:From August 1st to September 11th, 2014, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats in the southern Canadian Beaufort Sea and Amundsen Gulf as part of the Beaufort Regional Environmental Assessment (BREA) called the Beaufort Sea Marine Fishes Project (BSMFP). Sampling was conducted from the F/V Frosti at 54 stations along 14 transects. Standardized sampling was conducted at pre-determined depth stations (20-1500 m) using benthic trawling nets. The BSMFP is the first research project to use large research trawls to assess the biodiversity and abundance of offshore marine fishes in this area. In total, 42 species of fish were caught. This report contains information on fishing net deployments, catch per unit effort (CPUE), and basic biological data for all fishes caught.DESCRIPTION:Basic biological data for all fish caught during the 2014 BSMFP expedition. Includes identification, weight, length (total, fork, and, standard), liver weight, gonad weight, sex and maturity level. PARAMETERS COLLECTED:familyspeciesweighttotal lengthfork lengthstandard lengthliver weightgonad weightsexmaturitySAMPLING METHODS:Various trawl types.