Identification of significant concentrations of sponges in the Gulf of St. Lawrence biogeographic unit using Kernel density estimation (KDE).This method was applied to create a modelled biomass surface for each taxa and an aerial expansion method was permitted to identify significant concentrations. Only geo-referenced biomass data have been used to identify the “hot spots”. The borders of the areas were refined using knowledge of null catches and species distribution models. Predictive models were produced using a random forest machine-learning technique. For more details, please refer to this report: Kenchington, E., L. Beazley, C. Lirette, F.J. Murillo, J. Guijarro, V. Wareham, K. Gilkinson, M. Koen Alonso, H. Benoît, H. Bourdages, B. Sainte-Marie, M. Treble, and T. Siferd. 2016. Delineation of Coral and Sponge Significant Benthic Areas in Eastern Canada Using Kernel Density Analyses and Species Distribution Models. DFO Can. Sci. Advis. Sec. Res. Doc. 2016/093. vi + 178 p.http://waves-vagues.dfo-mpo.gc.ca/Library/40577806.pdfThe present layer only contains the analysis results for sponges. Purpose:As part of the Canada's commitment to the identification and protection of sensitive benthic marine ecosystems, maps of the location of significant concentrations of corals and sponges on the east coast of Canada were produced through quantitative analyses of research vessel trawl survey data, supplemented with other data sources where available. The taxa analyzed are sponges (Porifera), large and small gorgonian corals (Alcyonacea), and sea pens (Pennatulacea). However, only the sponges (Porifera) and sea pens (Pennatulacea) have been considered in the analysis concerning the Gulf of St. Lawrence biogeographic unit.

Identification of significant concentrations of sea pens in the Gulf of St. Lawrence biogeographic unit using Kernel density estimation (KDE).This method was applied to create a modelled biomass surface for each taxa and an aerial expansion method was permitted to identify significant concentrations. Only geo-referenced biomass data have been used to identify the “hot spots”. The borders of the areas were refined using knowledge of null catches and species distribution models. Predictive models were produced using a random forest machine-learning technique. For more details, please refer to this report: Kenchington, E., L. Beazley, C. Lirette, F.J. Murillo, J. Guijarro, V. Wareham, K. Gilkinson, M. Koen Alonso, H. Benoît, H. Bourdages, B. Sainte-Marie, M. Treble, and T. Siferd. 2016. Delineation of Coral and Sponge Significant Benthic Areas in Eastern Canada Using Kernel Density Analyses and Species Distribution Models. DFO Can. Sci. Advis. Sec. Res. Doc. 2016/093. vi + 178 p.http://waves-vagues.dfo-mpo.gc.ca/Library/40577806.pdfThe present layer only contains the analysis results for sea pens. Purpose:As part of the Canada's commitment to the identification and protection of sensitive benthic marine ecosystems, maps of the location of significant concentrations of corals and sponges on the east coast of Canada were produced through quantitative analyses of research vessel trawl survey data, supplemented with other data sources where available. The taxa analyzed are sponges (Porifera), large and small gorgonian corals (Alcyonacea), and sea pens (Pennatulacea). However, only the sponges (Porifera) and sea pens (Pennatulacea) have been considered in the analysis concerning the Gulf of St. Lawrence biogeographic unit.

Polygons denoting concentrations of sea pens, small and large gorgonian corals and sponges on the east coast of Canada have been identified through spatial analysis of research vessel survey by-catch data following an approach used by the Northwest Atlantic Fisheries Organization (NAFO) in the Regulatory Area (NRA) on Flemish Cap and southeast Grand Banks. Kernel density analysis was used to identify high concentrations and the area occupied by successive catch weight thresholds was used to identify aggregations. These analyses were performed for each of the five biogeographic zones of eastern Canada. The largest sea pen fields were found in the Laurentian Channel as it cuts through the Gulf of St. Lawrence, while large gorgonian coral forests were found in the Eastern Arctic and on the northern Labrador continental slope. Large ball-shaped Geodia spp. sponges were located along the continental slopes north of the Grand Banks, while on the Scotian Shelf a unique population of the large barrel-shaped sponge Vazella pourtalesi was identified. The latitude and longitude marking the positions of all tows which form these and other dense aggregations are provided along with the positions of all tows which captured black coral, a non-aggregating taxon which is long-lived and vulnerable to fishing pressures.These polygons identify sea pen fields from the broader distribution of sea pens in the region as sampled by Campelen trawl gear in the Newfoundland - Labrador Shelves biogeographic zone. A 0.4 kg minimum threshold for the sea pen catch was identified as the weight that separated the sea pen field habitat from the broader distribution of sea pens with these research vessel tow data and gear type.

Significant Benthic Areas are defined in DFO's Ecological Risk Assessment Framework (ERAF) as "significant areas of cold-water corals and sponge dominated communities", where significance is determined "through guidance provided by DFO-lead processes based on current knowledge of such species, communities and ecosystems". Here we provide maps of the location of significant concentrations of corals and sponges on the east coast of Canada produced through quantitative analyses of research vessel trawl survey data, supplemented with other data sources where available. We have conducted those analyses following a bio-regionalization approach in order to facilitate modelling of similar species, given that many of the multispecies surveys do not record coral and sponge catch at species level resolution. The taxa analyzed are sponges (Porifera), large and small gorgonian corals (Alcyonacea), and sea pens (Pennatulacea). We applied kernel density estimation (KDE) to create a modelled biomass surface for each of those taxa, and applied an aerial expansion method to identify significant concentrations, following an approach first applied in 2010 to this region. We compared our results to those obtained previously. KDE uses only geo-referenced biomass data to identify "hot spots". The borders of the areas so identified can be refined using knowledge of null catches and species distribution models that predict species presence-absence and/or biomass, both incorporating environmental data.

A research survey on American lobster (Homarus americanus) and rock crab (Cancer irroratus) has been carried out annually on the Magdalen Islands since 1995 to assess the abundance and structure of populations in this area. The survey is carried out during the first two weeks of September and covers the southern part of the Magdalen Islands, from "Baie de Plaisance" to "Grande-Entrée", extending slightly eastwards between "Havre-aux-Maisons" and "Grande-Entrée". The survey lasts 8 to 15 days, depending of the weather conditions, and covers a maximum of 48 stations. Approximately half of the stations are surveyed in duplicate (two tows per station). The tows always cover 500 meters, with a slightly variable trawl opening (as explained above), averaging around 10 meters.The gear used is a Nephrops trawl, a benthic trawl originally developed for langoustine fishing in Brittany, but slightly modified to target lobster. The trawl is deployed from the CCGS Leim and the harvested organisms are hauled aboard and sorted. Cephalothorax length, sex and shell stage are recorded for all lobsters. This is supplemented by information on reproductive conditions for certain size classes, and dissections of 25 males and 25 females per mission. Rock crabs are also included in the survey and are counted with additional measurements of shell width, sex and shell condition that are recorded on predetermined stations. For other stations individuals data are collected depending on the time available between trawl hauls.The publication contains 4 files; the "Information_stations" file which contains the data of the stations, the "Data_homard_lobster_survey" file which contains the measurements of each lobster, the "Nombre_crabe_crab_number" file which contains the count of rock crabs, the "Mesure_crabe_crab_measurement" file contains the characteristics measured for each crab. Each of the files can be linked by the "date", "st", "tr" columns. The “bat” column notes the boat code, because the gear is not deployed in the same way on each boat.*This record consist of raw data and quality has not been verifiedAssociated species are identified and semi-quantitatively counted directly on the sorting table, and the results are presented in the following publication: https://open.canada.ca/data/en/dataset/99cb7034-f3d5-4da4-a5bc-e81315cfb8eb

A research survey on American lobster (Homarus americanus) has been conducted annually in the Magdalen Islands since 1995 to assess the abundance and structure of lobster populations in this area. Starting in 2007, the main objective was modified to include the diversity and abundance of benthic species associated with lobster habitat. Only benthic species data associated with lobster habitat are presented in this dataset.Surveys were generally conducted during the first two weeks of September from 2007 to 2020 on the entire south side of the Magdalen Islands, from Grosse-Île to Havre-Aubert. The sampling plan consists of 70 trawl tows on 50 fixed stations, between 4 and 35 meters in depth. Some stations had double tows. Specimens were collected using a Nephrops-type door trawl with a total width of 3.04 meters consisting of four Vexar™ lined baskets of 19 millimeter mesh size in order to harvest small individuals. Start and end positions were recorded to calculate the distance traveled at each tow using the geosphere library in R. The year 2011 is not included in the dataset, as biodiversity data were not recorded. Since 2017, the average tow distance has been reduced from 1000 meters to 500 meters. The opening of the trawl is also noted every minute throughout the duration of the tow so that the area sampled can be calculated. The area covered in each tow was the product of the trawl opening and the distance traveled.The two files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "Event_data" file includes generic activity information, including date and location. The "Occurrence" file includes the taxonomy of the observed species, identified to the species or lowest possible taxonomic level. For abundance and biomass estimates, contact Benoît Bruneau (Benoit.Bruneau@dfo-mpo.gc.ca).For quality controls, all taxonomic names were checked against the World Register of Marine Species (WoRMS) to match recognized standards. The WoRMS match was placed in the "ScientificnameID" field of the occurrence file. Special cases were noted in the "identificationRemarks" field and selected specimens were confirmed with field photos mentioned in "associatedMedia". Data quality checks were performed using the R obistools and WORMS libraries. All sampling locations were spatially validated.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

An annual trawl survey is conducted in Southwestern Nova Scotia and the Bay of Fundy to assess the lobster stocks in the area. The survey is conducted with the Northeast Fisheries Science Center Ecosystem Survey Trawl (NEST), a small mesh trawl with a cod end liner, which ensures the capture of various sizes of lobster. The dimensions and location of the trawl are monitored and recorded throughout the tow using an electronic trawl mensuration system. In addition, water temperature and depth are also monitored. The target tow length is 1 kilometer which is tracked using an Olex marine charting system. Vessel crew, DFO science staff and a contracted at sea observer work together to perform required tasks and collect all relevant data. Catch from each tow is separated by species, weighed and counted. Length frequency data is collected on select groundfish and crab species and detailed morphometric data is collected on each lobster.PARAMETERS COLLECTED:Set information and profile - includes set date, time depth and locationCatch summary - weight and number caught of each speciesLength Freqeuncies - completed for up to 100 fish for selected speciesLobster Morphological Data - detailed data collected on each lobsterBottom TemperatureTrawl MetricsNOTES ON QUALITY CONTROL:Data is visually verified and double keypunched. On loading to Oracle, data is run through rigorous automated checks to verify data accuracy and integrity.SAMPLING METHODS:The target tow length is 1 kilometer which is tracked using an Olex marine charting system. Vessel crew, DFO science staff and a contracted at sea observer work together to perform required tasks and collect all relevant data. Catch from each tow is separated by species, weighed and counted. Length frequency data is collected on select groundfish and crab species, detailed morphometric data is collected on each lobster. Bottom temperature and trawl metrics are collected for each tow.CITATION LIST:Denton, Cheryl M.. 2020. Maritimes Region Inshore Lobster Trawl Survey Technical Description. Canadian technical reports of fisheries and aquatic sciences (DFO) 3376.

This project was completed by the Productive Capacity group (Coastal and Freshwater Ecology Section) in the Newfoundland and Labrador Science Branch of Fisheries and Oceans Canada (DFO). American lobster (Homarus americanus) is a commercially important decapod crustacean species along the east coast of North America, ranging from the Labrador coast south to Cape Hatteras. Juvenile lobster < 40 mm CL (carapace length) recruitment has been studied extensively in the southern portions of their range. However, investigations of settlement habitat association and recruitment of juvenile lobster in the northern extremes along the Newfoundland coast have been largely unsuccessful. We investigated juvenile lobster density, habitat, and depth associations in three areas of Newfoundland, using scuba – Port Saunders area, 8 dives conducted 28 September - 2 October 2017, and Burin Peninsula 10 dives (7 Placentia Bay, 3 Fortune Bay) conducted 30 September - 4 October 2018 over a total seabed area of 9,138 m2, within 200 meters of shore. Port Saunders and Fortune Bay had relatively higher lobster density (0.09 and 0.40 m-2, respectively); >65% were juveniles, in contrast to Placentia Bay where lobster densities of all size groups were low (mean 0.01 m-2) and no juvenile lobsters were observed at all. Where observed all juvenile lobster were significantly associated with shallow (<6 m) habitat and showed no overlap with distribution of adults (>82.5 mm CL) which we observed at depths 6 to 17 m. Our sites were dominated by varying mixes of cobble and pebble (77%); rock/bedrock (12%) and mud/sand/small pebble (11%) substrates interspersed with overlying kelp (32%) and eelgrass (11%) vegetation. We observed no significant associations with substrate or vegetation. This record contains the geographic locations of the 7 Placentia Bay sites surveyed, and information on the timing and type of data collected at each site, which was one component supported by Coastal Environmental Baseline Program of a larger collaborative project.

Location and contact information for Pacific Recreational Fishery Salmon Head Recovery Depots.The sport fishing community has an important role in the recovery of coded-wire tags found in Coho and Chinook. A coded-wire tag is a 1mm piece of wire that is laser etched with a unique number. Tags are injected into the nose cartilage of juvenile salmon prior to ocean migration. Annually, Canada and the United States tag over 50 million juvenile salmon. Fisheries and Oceans Canada applies about 5.5 million tags, using about 5.5 kilometres of wire. Anglers can recognize the presence of a coded-wire tag because of the missing adipose fin (located on the dorsal surface of the salmon). If you have caught an adipose fin clipped Coho and Chinook, it is a simple matter of removing the head from the fish, completing a sport head label and then submitting the head to a Sport Head Recovery Depot in the area. It is just as important to turn in heads from terminal or freshwater sites as it is from marine areas. Even though anglers fishing close to hatcheries can be fairly certain of the origin of their catch, data will not be recorded unless the heads from fin-clipped recoveries are turned in. Without the data, the health of the stock and the value of the resource to anglers could be underestimated.