Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

2014 to 2023 pelagic fish abundance in the Estuary and the Gulf of St. Lawrence. The abundance is expressed as a number of individuals per normalized trawl set. Each species is individually represented in the northern Gulf and in the southern Gulf. Input data are from the annual August (North) and September (South) multidisciplinary surveys. The pelagic species represented are: Arctic Cod, Atlantic Argentine, Atlantic Herring, Atlantic Mackerel, Atlantic Soft Pout, Capelin, Lumpfish, Pollock, Rainbow Smelt, Sand Lances, Silver Hake, Threespine Stickleback and White Barracudina.PurposeSince 1990, the Department of Fisheries and Oceans has been conducting an annual multidisciplinary survey in the Estuary and northern Gulf of St. Lawrence using a standardized protocol. In the southern Gulf of St. Lawrence, these bottom trawl surveys has been carrying out each September since 1971. These missions are an important source of information about the status of the marine ressources.The objectives of the surveys are multiple: to estimate the abundance and biomass of groundfish and invertebrates, to identify the spatial distribution and biological characteristics of these species, to monitor the biodiversity of the Estuary and Gulf and finally, to describe the environmental conditions observed in the area at the moment of the sampling.The southern Gulf surveys are realized using the following standardized protocol:Hurlbut,T. and D.Clay (eds) 1990. Protocols for Research Vessel Cruises within the Gulf Region (Demersal Fish) (1970-1987). Can. MS Rep. Fish. Aquat. Sci. No. 2082: 143p.The sampling protocols used for the Estuary and northern Gulf surveys are described in details in the following publications:Bourdages, H., Archambault, D., Bernier, B., Fréchet, A., Gauthier, J., Grégoire, F., Lambert, J., et Savard, L. 2010. Résultats préliminaires du relevé multidisciplinaire de poissons de fond et de crevette d’août 2009 dans le nord du golfe du Saint-Laurent. Rapp. stat. can. sci. halieut. aquat. 1226 : xii+ 72 p. Bourdages, H., Archambault, D., Morin, B., Fréchet, A., Savard, L., Grégoire, F., et Bérubé, M. 2003. Résultats préliminaires du relevé multidisciplinaire de poissons de fond et de crevette d’août 2003 dans le nord du golfe du Saint-Laurent. Secr. can. consult. sci. du MPO. Doc. rech. 2003/078. vi + 68 p.Annual reports are available at the Canadian Science Advisory Secretariat (CSAS), (http://www.dfo-mpo.gc.ca/csas-sccs/index-eng.htm).Bourdages, H., Brassard, C., Desgagnés, M., Galbraith, P., Gauthier, J., Légaré, B., Nozères, C. and Parent, E. 2017. Preliminary results from the groundfish and shrimp multidisciplinary survey in August 2016 in the Estuary and northern Gulf of St. Lawrence. DFO Can. Sci. Advis. Sec. Res. Doc. 2017/002. v + 87 p.

The North Shore of the Lower Estuary (Upper North Shore, Quebec) is a productive coastal system where many commercial species of benthic invertebrates are fished in the infralittoral (10-20 m) and circalittoral (20-50 m) zone. However, little data exist on the biodiversity of non-commercial species and the environmental characteristics of the benthic habitat in this area. Two scientific surveys were conducted in 2018 and 2019 to address this knowledge gap by developing a framework of biodiversity and environmental (water column and seafloor) data taking that will be used to determine the baseline state of the benthic ecosystem in this region.Surveys were conducted in 2018 (August 11-14) and 2019 (July 30-August 5) in the Upper North Shore region (between the towns of Forestville and Godbout). Surveys followed a fixed sampling design of eight transects perpendicular to bathymetry with stations at 10 m depth intervals in a bathymetry range of 10-50 m for a total of approximately 40 stations per survey. Specimens were collected with a beam trawl with an opening of 2.8 m. The hauls were made at a target speed of 2 knots and a target duration of 7 minutes. Start and end positions were recorded to calculate the distance traveled on each tow using the geosphere library of R. The average tow distance was approximately 425 m. The area covered at each tow was the product of the trawl opening and the distance traveled. The three files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "event_information" file includes generic event information, including date and location. The "additional_information_event_and_occurrence" file includes sample size, sampling protocol and sampling effort, among others. The "taxon_occurrence" file includes the taxonomy of the species observed, identified to the species or lowest possible taxonomic level. For abundance and biomass estimates, contact Virginie Roy (virginie.roy@dfo-mpo.gc.ca). For quality controls, all taxonomic names were checked against the World Register of Marine Species (WoRMS) to match recognized standards. The WoRMS match was placed in the "scientificNameID" field of the occurrence file. Special cases were noted in "identificationRemarks" and selected specimens were confirmed using field photos. Data quality checks were performed using the R obistools and worrms libraries. All sampling locations were spatially validated.This project was funded by DFO Coastal Environmental Baseline Program under Canada’s Oceans Protection Plan. This initiative aims to acquire environmental baseline data contributing to the characterization of important coastal areas and to support evidence-based assessments and management decisions for preserving marine ecosystems.

A research survey on snow crab (Chionoecetes opilio) was conducted from May 2006 to May 2009 in the Bay of Ste. Marguerite near Sept-Îles, Quebec. The main objective of this survey was to assess the abundance of snow crab and benthic species associated with snow crab habitat. Only data for benthic species associated with snow crab habitat are presented in this dataset.Data were collected according to a fixed station sampling design consisting of 79 stations, between 7 to 198 meters depth. Specimens were collected using a beam trawl. The codend was lined with a small stretched mesh net in order to harvest the small individuals. The hauls were made at a target duration of 15 minutes. Start and end positions were recorded to calculate the distance traveled on each tow using the geosphere library in R. The two files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "Activity_Information" file includes generic activity information, including date and location. The "occurrence_taxon" file includes the taxonomy of the species observed, identified to the species or lowest possible taxonomic level. To obtain the abundance and biomass assessment, contact Bernard Sainte-Marie (Bernard.Sainte-Marie@dfo-mpo.gc.ca).For quality controls, all taxonomic names were checked against the World Register of Marine Species (WoRMS) to match recognized standards. The WoRMS match was placed in the "ScientificnameID" field of the occurrence file. Data quality checks were performed using the R obistools and worrms libraries. All sampling locations were spatially validated.

The Ocean Data Inventory database is an inventory of all of the oceanographic time series data held by the Ocean Science Division at the Bedford Institute of Oceanography. The data archive includes about 5800 current meter and acoustic doppler time series, 4500 coastal temperature time series from thermographs, as well as a small number (200) of tide gauges. Many of the current meters also have temperature and salinity sensors. The area for which there are data is roughly defined as the North Atlantic and Arctic from 30° - 82° N, although there are some minor amounts of data from other parts of the world. The time period is from 1960 to present. The database is updated on a regular basis.

The layer presents the information on the distribution of eelgrass (Zostera marina) beds in James Bay, Chaleur Bay, Estuary and Gulf of St. Lawrence according to a literature review of documents produced between 1987 and 2009. Additional InformationEelgrass's inventory was produced according to a literature review of the following documents:Calderón, I. 1996. Caractérisation de la végétation et de la faune ichtyenne de la baie de Sept-Îles. Document réalisé par la Corporation de protection de l'environnement de Sept-Îles pour Pêches et Océans Canada. 26p. + 5 annexes.Comité côtier Les Escoumins à la Rivière Betsiamites. 2004. Inventaire de localisation des bancs de zostère marine dans la zone côtière Les Escoumins à la rivière Betsiamites. 9 p.Comité ZIP Côte-Nord du Golfe. 2001. Inventaire du potentiel côtier et marin de la Basse-Côte-Nord. Version préliminaire de rapport sous forme de CD-ROM, Sept-Îles, mars 2001.Comité ZIP de la rive nord de l’estuaire. 2008. Guide d’intervention en matière de protection et de mise en valeur des habitats littoraux d’intérêt de la rive nord de l’estuaire maritime (fiches 14 à 20). 8 p. + 7 fiches + annexe.Conseil Régional de l’Environnement Gaspésie et des Îles-de-la-Madeleine (2004). Inventaire et étude des bancs de zostère marine sur le territoire couvert par les comités de gestion intégrée de la zone côtière de l’Est du Québec. CONSORTIUM GAUTHIER & GUILLEMETTE - G.R.E.B.E. 1992. Description et cartographie des habitats côtiers de la Baie de Hannah jusqu'à la rivière au Castor. Rapport présenté à Hydro-Québec, Complexe Nottaway-Broadback-Rupert (NBR), Vol. 2, Annexe cartographique.Giguère, M., C. Duluc, S. Brulotte, F. Hazel, S. Pereira et M. Gaudet. 2006. Inventaire d’une population d'huître américaine (Crassostrea virginica) dans le Bassin aux Huîtres aux Îles-de-la-Madeleine en 2005. Rapport manuscrit. vi + 21 p.Grant, C. et L. Provencher, 2007. Caractérisation de l’habitat et de la faune des herbiers de Zostera marina (L.) de la péninsule de Manicouagan (Québec). Rapp. tech. can. sci. halieut. aquat. 2772 : viii + 65 p. Groupe Environnement Littoral. 1992. Complexe NBR. La zostère marine. Rapport présenté à la vice-présidence Environnement d'Hydro-Québec. 9 p. + 2 cartes.Harvey, C. et D. Brouard. 1992. Étude exploratoire du barachois de Chandler: aspects biophysiques et contamination. Rapport présenté à Environnement Canada, Direction de la protection de l'environnement région du Québec. 39 p. et annexes.Hazel, François, 2002. Données de terrain prises par F. Hazel, Septembre 2002.Ellefsen, H.-F. 2009. Communication personnelle de Hans-Frédéric Ellefsen (MPO).Jacquaz et coll. 1990. Étude biophysique de l'habitat du poisson de quatre barachois de la baie des Chaleurs.Kedney, G. et P. Kaltenback. 1996. Acquisition de connaissances et mise en valeur des habitats du banc de Portneuf. Document réalisé par la firme Pro Faune pour le Comité touristique de Rivière-Portneuf. 50 pages et 5 annexes.Lalumière, R. 1987. Répartition de la zostère marine (Zostera marina) sur la côte est de la baie James; été 1987. Rapport produit par Gilles Shooner et Associés inc. pour la Société d’énergie de la Baie James. 30 p. et annexes.Lalumière, R., L. Belzile et C. Lemieux. 1992. Étude de la zostère marine le long de la côte nord-est de la baie James (été 1991). Rapport présenté au Service écologie de la SEBJ. 31 p. + carte.Leblanc, J. 2002. Communication personnelle de Judith Leblanc (MPO).Lemieux, C. 1995. Acquisition de connaissances des habitats côtiers dans la région de Rimouski (1995). Rapport du Groupe-Conseil GENIVAR présenté au Ministère des Pêches et des Océans du Canada, Division de la Gestion de l’Habitat du Poisson, 52 pages + 2 annexes.Lemieux, C. et R. Lalumière. 1995. Acquisition de connaissances des habitats côtiers du barachois de Saint-Omer. Rap. du Groupe conseil Genivar inc. pour la DGHP, MPO, 44 pages + 3 ann.Martel, Marie-Claude, Lizon Provencher, Cindy Grant, Hans-Frédéric Ellefsen et Selma Pereira, 2009. Distribution and description of eelgrassbeds in Québec. Fisheries and Oceans Canada, Canadian Science Advisory Secretariat, Research Document 2009/050. 45p. Morin, D. 2009. Communication personnelle de Danièle Morin (MRNF).Naturam Environnement. 1999. Caractérisation biophysique, socio-économique et détermination des enjeux dans un secteur potentiel pour l’identification d’une zone de protection marine pilote: portion ouest de la MRC Manicouagan. Baie-Comeau. 311 p. Pelletier, Claudel. 2003. Communication personnelle de Claudel Pelletier, FAPAQ, lettre en date du 24 février 2003.Pereira, S. 2009. Communication personnelle de Selma Pereira (MPO).Vaillancourt, M.-A. et C. Lafontaine. 1999. Caractérisation de la Baie Mitis. Jardins de Métis et Pêches et Océans Canada. Grand-Métis. 185 p.

During the period of 1987 to 1994, Robin J. LeBrasseur and N. Brent Hargreaves lead a juvenile salmon predation research project in Alberni Inlet and Barkley Sound BC. This dataset contains the research survey catch data and individual fish examinations data.

This inventory, conducted from September 26th to October 3th, 2019, aimed to describe the community structure of macroalgae and benthic macroinvertebrates of five small estuaries of the Upper North Shore of Quebec, namely Barthélemy Bay and the Colombier, Mistassini, Franquelin and Saint-Nicolas rivers. This inventory is part of a doctoral study of Valentine Loiseau on the global changes in the St. Lawrence system, mainly the study of marine benthic communities in response to changes of salinity, to ensure proper management of the environment in the face of future changes. The main objective is to describe the structure and the levels of specific diversities of mediolittoral communities of benthic macroinvertebrates and macroalgae along a salinity gradient. These five small estuaries were selected because of their similar size, hard substrates and easy access. Three levels of hypoosmotic stress (low, medium, high) and one control level (seawater) were used for each of the selected estuaries, with eight quadrats per stress level. Quadrat positions were randomly selected but had to meet two criteria: (1) regular height in the foreshore to control the influence of other stresses (temperature, exposure); and (2) presence of at least one macroalga to maintain homogeneity. A percentage cover by macroalgal and macroinvertebrate species was estimated, and then all organisms were weighed by species and size group. The salinity of the nearest water point was measured at mid-tide with a portable refractometer and a Castaway-type CTD (Conductivity-Temperature-Density) probe. The inventory was done using a stratified random sampling design and the sampling unit was a quadrat measuring 25 x 25 cm. The three files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "event_information" file includes the generic information of the quadrat, including date and location. The "additional_information_event_and_occurrence" file includes salinity and substrate type of the quadrat, as well as the total weight of all individuals of the same species caught in the quadrat extrapolated to one square metre of surface. For nudibranchs and barnacles, weight was estimated from the size of the individuals so that they were not removed from the environment. The "taxon_occurrence" file includes the taxonomic inventory of macroalgal and benthic macroinvertebrate species observed in the quadrat, identified to the lowest possible species or taxonomic level and biomass by identified species.For quality control, organisms were identified on the field using the following guide: Chabot, Robert et Anne Rossignol. 2003. Algues et faune du littoral du Saint-Laurent maritime : Guide d'identification. Institut des Sciences de la mer de Rimouski, Rimouski ; Pêches et Océans Canada (Institut Maurice-Lamontagne), Mont-Joli. 113 pages. The taxonomy was checked against the World Register of Marine Species (WoRMS) to match recognized standards and using the R obistools and worrms libraries. The WoRMS match was placed in the "scientificNameID" field of the occurrence file. All sample locations were spatially validated. This project was funded by DFO Coastal Environmental Baseline Program under Canada’s Oceans Protection Plan. This initiative aims to acquire environmental baseline data contributing to the characterization of important coastal areas and to support evidence-based assessments and management decisions for preserving marine ecosystems.

Polygons denoting concentrations of sea pens, small and large gorgonian corals and sponges on the east coast of Canada have been identified through spatial analysis of research vessel survey by-catch data following an approach used by the Northwest Atlantic Fisheries Organization (NAFO) in the Regulatory Area (NRA) on Flemish Cap and southeast Grand Banks. Kernel density analysis was used to identify high concentrations and the area occupied by successive catch weight thresholds was used to identify aggregations. These analyses were performed for each of the five biogeographic zones of eastern Canada. The largest sea pen fields were found in the Laurentian Channel as it cuts through the Gulf of St. Lawrence, while large gorgonian coral forests were found in the Eastern Arctic and on the northern Labrador continental slope. Large ball-shaped Geodia spp. sponges were located along the continental slopes north of the Grand Banks, while on the Scotian Shelf a unique population of the large barrel-shaped sponge Vazella pourtalesi was identified. The latitude and longitude marking the positions of all tows which form these and other dense aggregations are provided along with the positions of all tows which captured black coral, a non-aggregating taxon which is long-lived and vulnerable to fishing pressures.These polygons identify sea pen fields from the broader distribution of sea pens in the region as sampled by Western II A trawl gear in the Scotian Shelf biogeographic zone. A 0.1 kg minimum threshold for the sea pen catch was identified as the weight that separated the sea pen field habitat from the broader distribution of sea pens with these research vessel tow data and gear type.