Bay-scale empirical demonstrations of how bivalve aquaculture alters plankton composition, and subsequently ecological functioning and higher trophic levels, are lacking. Temporal, inter- and within-bay variation in hydrodynamic, environmental, and aquaculture pressure limit efficient plankton monitoring design to detect bay-scale changes and inform aquaculture ecosystem interactions. Here, we used flow cytometry to investigate spatio-temporal variations in bacteria and phytoplankton (< 20 µm) composition in four bivalve aquaculture embayments. We observed higher abundances of bacteria and phytoplankton in shallow embayments that experienced greater freshwater and nutrient inputs. Depleted nutrient conditions may have led to the dominance of picophytoplankton cells, which showed strong within-bay variation as a function of riverine vs freshwater influence and nutrient availability. Although environmental forcings appeared to be a strong driver of spatio-temporal trends, results showed that bivalve aquaculture may reduce near-lease phytoplankton abundance and favor bacterial growth. We discuss aquaculture pathways of effects such as grazing, benthic-pelagic coupling processes, and microbial biogeochemical cycling. Conclusions provide guidance on optimal sampling considerations using flow cytometry in aquaculture sites based on embayment geomorphology and hydrodynamics.Cite this data as: Sharpe H, Lacoursière-Roussel A, Barrell J (2024). Monitoring bay-scale bivalve aquaculture ecosystem interactions using flow cytometry. Version 1.2. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=monitoring_bay-scale_bivalve_aquaculture_ecosystem_interactions_using_flow_cytometry&v=1.2

Bivalve aquaculture has direct and indirect effects on plankton communities, which are highly sensitive to short-term (seasonal, interannual) and long-term climate changes, although how these dynamics alter aquaculture ecosystem interactions is poorly understood. Here, we investigate seasonal patterns in plankton abundance and community structure spanning several size fractions from 0.2 µm up to 5 mm, in a deep aquaculture embayment in northeast Newfoundland, Canada. Using flow cytometry and FlowCam imaging, we observed a clear seasonal relationship between fraction sizes driven by water column stratification (freshwater input, nutrient availability, light availability, water temperature). Plankton abundance decreased proportionally with increasing size fraction, aligning with size spectra theory. Within the bay, greater mesozooplankton abundance, and a greater relative abundance of copepods, was observed closest to the aquaculture lease. No significant spatial effect was observed for phytoplankton composition. While the months of August to October showed statistically similar plankton composition and size spectra slopes (i.e., food chain efficiency) and could be used for interannual variability comparisons of plankton composition, sampling for longer periods could capture long-term phenological shifts in plankton abundance and composition related to various processes, including climate change. Conclusions provide guidance on optimal sampling to monitor and assess aquaculture pathways of effects.Cite this data as: Sharpe H, Lacoursière-Roussel A, Gallardi D (2024). Ecological insight of seasonal plankton succession to monitor shellfish aquaculture ecosystem interactions. Version 3.2. Fisheries and Oceans Canada. Sampling event dataset. https://doi.org/10.25607/2ujdvh

The Nova Scotia Department of Fisheries and Aquaculture is the lead regulatory of aquaculture leasing and licensing. This file illustrates all the issued landbased licenses within Nova Scotia. Fisheries and Aquaculture also provides a mapping tool for this data at: http://novascotia.ca/fish/aquaculture/site-mapping-tool/

The project (Quoddy Region Pelagics Telemetry) will support the assessment of the effects of aquaculture on the distribution and abundance of pelagic fishes (salmon, mackerel, herring) and large predators (shark, marine mammals) in Passamaquoddy Bay and the Bay of Fundy, an area of intense finfish culture. An acoustic receivers network is placed yearly (from April to December) across various passageways, locations of project-specific interest, and at aquaculture sites in the region. Tagged pelagic species will be tracked through the network to provide information on migration routes, movement speed, survival rates and suspected predators, and determine interaction and residence at aquaculture sites. The network was utilized for monitoring the passage of: hatchery-reared wild salmon (n=340) released in the Magaguadavic River in 2018, 2019 and 2021, wild alewives (n=30) from the St. Croix River in 2021, and farmed Atlantic salmon released in the wild (n=99) in 2021. The receiver network has more recently supported adjacent projects on the use of the region by white shark and porbeagle as well as the residence of mackerel, herring, and sculpin at farm sites. The receivers additionally support other researchers with detection of striped bass, Inner Bay of Fundy Atlantic salmon, sturgeon, and many other species. Placement of the network will continue into 2025 inclusive with the longer-term goal to eventually deploy an array covering the entrance to the Bay of Fundy.Cite this data as: Trudel, M., Wilson, B., Black, M. 2023. Assessing bay-scale impacts of aquaculture operations on the distribution and abundance of pelagic fishes and large predators. Accessed via the Ocean Tracking Network OBIS IPT in January 2025 (version 3.1). https://doi.org/10.14286/xfa6sr

Provides access to information about marine aquaculture sites for shellfish. Users are able to view all marine aquaculture sites situated in tidal waters of New Brunswick and determine their Lease number, current status and area (in m2). The FAQ for the data can be found at https://www2.gnb.ca/content/dam/gnb/Departments/10/pdf/Aquaculture/MASMP_FrequentlyAskQuestuions.pdf/Fournit un accès à des renseignements relatifs aux sites aquacoles marins pour les mollusques. Les utilisateurs peuvent voir tous les sites aquacoles marins situés dans les eaux de marée du Nouveau-Brunswick et déterminer le numéro de la concession à bail, sa situation actuelle et sa superficie (en m2). La FAQ concernant les données se trouve ici https:

Environmental Monitoring Program data for aquaculture.

The boundaries of a unit of a Shellfish Aquaculture Plan within a Coastal Plan

Map of harbours critical to fishing and aquaculture industries managed by harbour authorities (Core fishing harbours), harbours that support fishing and aquaculture industries that aren’t managed by harbour authorities (Non-core fishing harbours), and harbours that support the recreational community (Recreational harbours).

The Nova Scotia Department of Fisheries and Aquaculture is the lead regulatory of rockweed leasing and licensing. This file illustrates all the issued rockweed leases along the coast of Nova Scotia. Fisheries and Aquaculture also provides a Nova Scotia Aquaculture and Rockweed Map viewer at: https://fishaqua.maps.arcgis.com/apps/instant/media/index.html?appid=a7c3520e73894539afadf8602ee57ef1

As part of the development of a nationally-consistent sampling design within the Aquaculture Monitoring Program (AMP), this data reports mesozooplankton assemblages observed at nine coastal shellfish aquaculture sites, located across four DFO regions, with sampling across months, tide phases, and sampling locations. In most sites, strong spatial effects were observed, while tide effects were generally less important for structuring the mesozooplankton communities. Seasonality emerged as an essential factor to design an efficient monitoring program. This dataset represents the first large-scale Canadian coastal study using imaging technology for plankton taxonomic Identification.Cite this data as: Finnis, S., Guyondet, T., McKindsey, C.W., Arseneau, J., Barrell, J., Duhaime, J., Filgueira, R., Gallardi, D., Gaspard, D., Gibb, O., Goodwin, C., Hua, K., Macdonald, T., Milne, R., Lacoursière-Roussel, A. 2023. Guidance on sampling effort to monitor mesozooplankton communities at Canadian bivalve aquaculture sites using an optical imaging system. Can. Tech. Rep. Fish. Aquat. Sci. 3581: vii + 101 p