In 2012 and 2013, Fisheries and Oceans Canada surveyed the benthos in two areas closed to bottom contact fishing, the Narwhal Overwintering and Coldwater Coral Zone (now the Disko Fan Conservation Area, DFCA), and the Hatton Basin Voluntary Coral Protection Zone (now the Hatton Basin Conservation Area, HBCA). Samples were collected following protocols recommended by the Arctic Council’s Circumpolar Biodiversity Monitoring Plan for the purposes of providing baseline data for future monitoring of benthic invertebrates in this sensitive region, and for facilitating pan-Arctic comparisons of benthic communities. Five biodiversity monitoring stations were established, four in the DFCA and one in the HBCA, each of which was fully sampled according to those protocols with Van Veen grabs or box corers, drop cameras and temperature recorders attached to the gear. This report summarises the grab/core-sampled benthic fauna collected during the 2012 survey of the Conservation Areas and complements another report documenting the epibenthos from the camera transects in the DFCA. Here we report on macrofauna in the 1-cm size fraction, and on foraminiferan meiofauna.The data provided is presented in the following report (see related link) :Jacobs, K., Bouchard Marmen, M., Rincón, B., MacDonald, B., Lirette, C., Gibb, O., Treble, M., and Kenchington, E. 2022. Biodiversity Monitoring Stations for Benthic Macrofauna and Meiofauna in the Disko Fan and Hatton Basin Conservation Areas. Can. Tech. Rep. Fish. Aquat. Sci. 3487: vi + 86 p.Cite this data as: Bouchard Marmen, Marieve; Rincon, Beatriz ; MacDonald, Barry; Lirette, Camille; Gibb, Olivia; Treble, Margaret ; Jacobs, Kevin; Kenchington, Ellen (2022). Biodiversity Monitoring Stations for Benthic Macrofauna and Meiofauna in the Disko Fan and Hatton Basin Conservation Areas. Published January 2023. Ocean Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/b7bcff18-698b-4d40-a7bd-13d39925cbeb

This resource documents a dataset of epifauna occurrences collected in 2021 during The Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) program developed by the Department of Fisheries and Oceans Canada (DFO) in collaboration with university partners. The overall objective of KEBABB is to characterize the variability and trends in physical, chemical, and biological oceanographic conditions and food webs supporting fisheries in the connected ecosystems of western Baffin Bay and Lancaster Sound. In 2021, DFO expanded the KEBABB program to Barrow Strait (KEBABS-Knowledge and Ecosystem-Based Approach in Barrow Strait), a key productive area of the Tallurutiup Imanga National Marine Conservation Area. The study took place in the Eastern Canadian Arctic (mainly in Baffin Bay, Davis Strait and Barrow Strait). Sampling is done along transects at fixed stations in the study area. Catches are collected with a 1.5 m Agassiz trawl (5 mm mesh net) for 3 minutes bottom-contact time at a target speed of 1.5 knots and with a 3 m benthic beam trawl (6.4 mm mesh net) for 15 minutes bottom-contact time at a target speed of 3 knots. A total of 16 stations were sampled for epifauna in 2021 between 85-850 m depth. Epibenthic invertebrates are identified to the lowest possible taxonomic level and photographed. All unknown specimens are frozen. In the lab, the identifications are validated or refined with the photos and the frozen specimens.The data are presented in Darwin Core and are separated in two files:The “Activité_épifaune_KEBABB_epifauna_event_en” file which contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The “Occurrence_épifaune_KEBABB_epifauna_en” file that contains the taxonomic occurrences.Further details on sampling can be found in the following report: Pućko, M., Charette, J., Tremblay P., Brulotte S., St-Denis B., Ciastek S., Hedges, K., Kuzyk, Z., Roy V., and Michel, C. 2022. An ecosystem-based approach in the eastern Arctic: KEBABB/S (Knowledge and Ecosystem-Based Approach in Baffin Bay/Barrow Strait) 2021 expedition report. Can. Manuscr. Rep. Fish. Aquat. Sci. 3250: viii + 58 p. https://publications.gc.ca/collections/collection_2022/mpo-dfo/Fs97-4-3250-eng.pdfUSE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

This dataset documents the epifauna occurrences collected from 2021 to 2024 during the Canadian Beaufort Sea Marine Ecosystem Assessment (CBS-MEA) conducted by the Department of Fisheries and Oceans (DFO). This scientific program focuses on the integration of oceanography, food web linkages, physical-biological couplings, and spatial and interannual variabilities. The program also aims to expand the baseline coverage of species diversity, abundances, and habitat associations in previously unstudied areas of the Beaufort Sea and Western Canadian Archipelago. The study took place mainly in the Canadian Beaufort Sea and the Amundsen Gulf. Sampling is done along transects at fixed stations in the study area. Catches are collected with a 3 m benthic beam trawl for 10 minutes bottom-contact time at a target speed of 2 knots and with a modified Atlantic Western IIA otter trawl for 20 minutes bottom-contact time at a target speed of 2.9 knots. A total of 32 stations were sampled for epifauna in 2021, 22 in 2022, 23 in 2023 and 22 in 2024, between 22-655 m depth. Epibenthic invertebrates were identified to the lowest taxonomic level possible and photographed. All unknown specimens are frozen. In the lab, the identifications are validated or refined with the photos and the frozen specimens.The data are presented in Darwin Core and are separated in two files:The "Activité_épifaune_CBSMEA_epifauna_event_en" file which contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The "Occurrence_épifaune_CBSMEA_epifauna_en" file that contains the taxonomic occurrences.

Fisheries and Oceans Canada (DFO) selected the Manicouagan Peninsula area as a site of interest for the possible establishment of a Marine Protected Area (MPA) in 1998. With this in mind, DFO conducted surveys to assess benthic biodiversity between 2006 and 2008. During the various campaigns, more than a hundred stations were sampled using various tools to target epibenthic and endobenthic species. Various tools were used during the three years of sampling, including an SM (Smith-McIntyre) grab with an area of 0.1 m² and a height of 20 cm, an IKU-type grab with a surface area of 1.5 m² and a height of 50 cm, and a hydraulic dredge measuring 1.36 m wide and 0.66 m high with a net mesh size of 2.3 cm. As the dredge clogged fairly quickly, small organisms were collected in abundance. In 2006 and 2007, a camera mounted on a sled pulled by a boat along transects of varying lengths depending on the sampling campaign collected images (3,008 x 2,000 pixels) every 10 seconds, representing a seabed area of 40 x 40 cm.The data are presented in Darwin Core format in the file “donnees_manicouagan_data” The available data include taxonomic identifications of samples (individuals collected or identified in images) as well as metadata related to stations, including sampling date, depth, and geographic coordinates. Please note that the depths are for informational purposes only, since the bathymetry is very steep in this region.Further details on sampling can be found in the following report: Provencher, L et Nozères, C. 2011. Protocole de suivi des communautés benthiques de la zone de protection marine Manicouagan. Secr. can. de consult. sci. du MPO. Doc. de rech. 2011/051. iv +25 p. https://waves-vagues.dfo-mpo.gc.ca/library-bibliotheque/344357.pdf

The North Shore of the Lower Estuary (Upper North Shore, Quebec) is a productive coastal system where many commercial species of benthic invertebrates are fished in the infralittoral (10-20 m) and circalittoral (20-50 m) zone. However, little data exist on the biodiversity of non-commercial species and the environmental characteristics of the benthic habitat in this area. Two scientific surveys were conducted in 2018 and 2019 to address this knowledge gap by developing a framework of biodiversity and environmental (water column and seafloor) data taking that will be used to determine the baseline state of the benthic ecosystem in this region.Surveys were conducted in 2018 (August 11-14) and 2019 (July 30-August 5) in the Upper North Shore region (between the towns of Forestville and Godbout). Surveys followed a fixed sampling design of eight transects perpendicular to bathymetry with stations at 10 m depth intervals in a bathymetry range of 10-50 m for a total of approximately 40 stations per survey. Specimens were collected with a beam trawl with an opening of 2.8 m. The hauls were made at a target speed of 2 knots and a target duration of 7 minutes. Start and end positions were recorded to calculate the distance traveled on each tow using the geosphere library of R. The average tow distance was approximately 425 m. The area covered at each tow was the product of the trawl opening and the distance traveled. The three files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "event_information" file includes generic event information, including date and location. The "additional_information_event_and_occurrence" file includes sample size, sampling protocol and sampling effort, among others. The "taxon_occurrence" file includes the taxonomy of the species observed, identified to the species or lowest possible taxonomic level. For abundance and biomass estimates, contact Virginie Roy (virginie.roy@dfo-mpo.gc.ca). For quality controls, all taxonomic names were checked against the World Register of Marine Species (WoRMS) to match recognized standards. The WoRMS match was placed in the "scientificNameID" field of the occurrence file. Special cases were noted in "identificationRemarks" and selected specimens were confirmed using field photos. Data quality checks were performed using the R obistools and worrms libraries. All sampling locations were spatially validated.This project was funded by DFO Coastal Environmental Baseline Program under Canada’s Oceans Protection Plan. This initiative aims to acquire environmental baseline data contributing to the characterization of important coastal areas and to support evidence-based assessments and management decisions for preserving marine ecosystems.

This dataset documents the infauna occurrences collected from 2021 to 2023 during the Canadian Beaufort Sea Marine Ecosystem Assessment (CBS-MEA) conducted by the Department of Fisheries and Oceans (DFO). This scientific program focuses on the integration of oceanography, food web linkages, physical-biological couplings, and spatial and interannual variabilities.The program also aims to expand the baseline coverage of species diversity, abundances, and habitat associations in previously unstudied areas of the Beaufort Sea and Western Canadian Archipelago. The study took place mainly in the Canadian Beaufort Sea and the Amundsen Gulf. Sampling is done along transects at fixed stations in the study area. Catches are collected using a 50 x 50 cm box-corer. 2 or 3 box core is collected per station to obtain replicates. A total of 29 stations were sampled for infauna in 2021, 15 in 2022 and 25 in 2023 between 10-653 m depth. Half of the box corer (0.125 m2) is sampled for infauna taxonomy. The first 20 cm of sediment are collected and sieved through a 0.5 mm mesh sieve. The samples are preserved in seawater-formaldehyde solution (10 % v/v). In the lab, infauna is identified to the lowest taxon level possible.The data are presented in two files:The "Activité_endofaune_CBSMEA_infauna_event_en" file which contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The "Occurrence_endofaune_CBSMEA_infauna_en" file that contains the taxonomic occurrences.

This dataset contains the abundance (per m²) and the biomass (mg dry per m²) of macrofauna (≥ 500µm) in eelgrass and adjacent bare soft sediments, collected at sites in the Atlantic of Nova Scotia from 2009 to 2013.Cite this data as: Wong M.C. Data of Benthic invertebrates in seagrass and bare soft sediments in Atlantic Nova Scotia Published May 2020. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/05d5f46a-7f19-11ea-8a4e-1860247f53e3Publications: Wong, M. C., & Dowd, M. (2021). Functional trait complementarity and dominance both determine benthic secondary production in temperate seagrass beds. Ecosphere. 12(11), e03794. https://doi.org/10.1002/ecs2.3794Wong, M. C. (2018). Secondary Production of Macrobenthic Communities in Seagrass (Zostera marina, Eelgrass) Beds and Bare Soft Sediments Across Differing Environmental Conditions in Atlantic Canada. Estuaries and Coasts, 41, 536–548. https://doi.org/10.1007/s12237-017-0286-2

Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes.Cite this data as: Jeffery, N., Rubidge, E., Abbott, C., Westfall, K., Stanley, R. (2024): Data of: eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment.Published: August 2024. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/43a91ba7-8025-4330-88db-db14022d729d

Benthic Marine Ecounits in coastal and offshore British Columbia. Benthic ecounits are intended to describe the sea bed and nearshore. Seven variables were selected to derive benthic ecounits: 1. Depth; 2. Slope; 3. Relief; 4. Temperature; 5. Exposure; 6. Current and 7. Substrate. CRIMS is a legacy dataset of BC coastal resource data that was acquired in a systematic and synoptic manner from 1979 and was intermittently updated throughout the years. Resource information was collected in nine study areas using a peer-reviewed provincial Resource Information Standards Committee consisting of DFO Fishery Officers, First Nations, and other subject matter experts. There are currently no plans to update this legacy data.

The purpose of the survey is to document and record habitat types and associated algae and marine invertebrate species in a variety of habitat types. Transect locations are randomly selected throughout the study area, which rotates between the north and south coasts of British Columbia on a biannual basis. Transects are laid perpendicular to the shoreline. A team of two divers swim the transect with data sheets to collect habitat, algae and marine invertebrate data as detailed below in the methods section. Data is keypunched in an MS Access database that can be queried for species observations and environmental information.This dataset includes three tables pulled from the original database containing observations by species, observations by quadrat, and additional header information for each observation. All three tables can be linked by the field HKey. Three lookup tables are included as well, one for algae, one for invertebrates, and one for substrates.