In 2009, DFO defined 12 marine bioregions across the three oceans bordering Canada to support its marine planning efforts, such as the establishment of networks of marine protected areas. However, these bioregions cover vast areas and exhibit significant ecological heterogeneity, especially along the coasts. Yet, this heterogeneity in coastal ecosystems often needs to be considered at the local scale, particularly for management and conservation purposes.The objective of this exercise is to subdivide the Estuary and Gulf of St. Lawrence (EGSL) bioregion for the province of Quebec into coastal sub-bioregions to better reflect local and regional coastal characteristics. The coastal classification presented in this report is based on the integration of four existing classification systems for the EGSL, which were not specifically designed for classifying coastal ecosystems. Integrating these classification systems into a single approach allowed us to define 13 coastal sub-bioregions for the EGSL. Data presented here are the limit of the 13 ecoregions made from this work. A technical report is available for more details. See supporting documents:Gendreau, Y., Narancic, B. et Bourassa, M-N. 2025. Classification écologique du territoire côtier de l’estuaire et du golfe du Saint-Laurent au Québec. Rapp. tech. can. sci. halieut. aquat. 0000 :v + 22p.

DFO has committed to establishing a representative network of Marine Conservation Areas collectively comprising 30% of the EEZ by 2030. The MCT 2.0 program was established in 2021 to help achieve this target and establish long-term monitoring within Canadian conservation areas. Information on the distribution of species within a protected area is essential for the management and monitoring of a site. Decisions made on zoning, risk assessment, activity approvals and management effectiveness all require information on the distribution and status of species. The Scotian Shelf-Bay of Fundy Bioregion spans the domain of the Maritimes Region, and within it there are 8 Marine Refuges (MR), 3 Marine Protected Areas (MPA), and 2 Areas of Interest (AOI). Environmental DNA (eDNA) offers a promising approach for biodiversity monitoring that has been gaining increased attention in the marine realm. Through sampling relatively small volumes of water (~3L), eDNA can capture of millions of fragments of eDNA on specialized filters that can then be sequenced to identify the species composition of an area. eDNA metabarcoding is based on the foundations of DNA barcoding, where species are identified solely by their unique DNA sequences. This method is relatively simple, efficient (with the ability to characterize both fish and invertebrate diversity), and non-invasive, meaning there is no disruption of sensitive benthic habitats or need to dissect tissue samples from captured fish and invertebrates. eDNA has been shown to be comparable to other biodiversity censusing techniques and has the potential to rapidly conduct biodiversity surveys over a relatively large area, such as the Eastern Shore Islands AOI. The DFO Maritimes summer multispecies trawl survey is one of the longest-running data series with applications in fisheries stock assessment, and one of the only sources of data for offshore MPA design and monitoring. In this project we pair eDNA samples collected within current and proposed conservation areas with trawl catch data to create a biodiversity and animal community monitoring time series for these areas. We use eDNA and trawl catch data as complementary tools, where trawl-based catch provides information on fish abundance, size distributions and sex, and eDNA provides information on genetic diversity and can detect cryptic and rare species. Cite this data as: Jeffery, N.W. Monitoring regional conservation areas using paired environmental DNA and research trawl surveys. Published: May 2026. Coastal Ecosystems Science Division, Maritimes Region, Fisheries and Oceans Canada, Dartmouth NS.

Pepin et al. (2014) stated that three nested spatial scales were identified as relevant for the development of ecosystem summaries and management plans: Bioregion, Ecosystem Production Unit (EPU), and Ecoregion. A bioregion is composed by one or more EPUs, while an EPU consists of a combination of ecoregions, which represent elements with different physical and biological characteristics based on the analytical criteria applied. Pepin et al. (2014) reported on the consolidation of data and analyses of ecoregion structure for the continental shelf areas from the Labrador Sea to the mid-Atlantic Bight and provided recommendations on the definition of EPUs in the NAFO Convention Area. The results of two K-means clustering analyses (one geographically constrained and one un-constrained) and expert knowledge (including and considering location of ecoregions, knowledge of the distribution of major marine resources and fish stocks, and geographic proximity for delineation/definition of potential management units) served as guides for evaluation by NAFO’s (North Atlantic Fisheries Organization) working group on ecosystem science and assessments (WG-ESA). The final consensus from the discussions identified eight (8) major EPUs that can serve as practical candidate management units (from the 50 m isobaths, where research vessel data were available, seaward to the 1500 m isobaths) that consist of the Labrador Shelf (NAFO subareas 2GH), the northeast Newfoundland Shelf (subareas 2J3K), the Grand Banks (subareas 3LNO), Flemish Cap (subarea 3M), the Scotian Shelf (subareas 4VnsWX), Georges Bank (parts of subareas 5Ze and 5Zw), the Gulf of Maine (subarea 5Y and part of 5Ze) and the mid-Atlantic Bight (part of subarea 5Zw and subareas 6ABC). Southern Newfoundland (subarea 3Ps) was not included in the original analysis because fall survey data were unavailable. However, it was later added as an EPU after additional analysis of the fish community structure and trends using survey data from the spring, which indicated that this area is heavily influenced by the surrounding EPUs (NAFO 2015).The proposed candidate management units correspond to the EPUs that define major areas within the bioregions which contain a reasonably well defined food web/production system. The working group noted that the consensus solution represents a compromise that aims to define management units based on the boundaries of existing NAFO subareas that are appropriate for estimation of ecosystem and fishery production. References: NAFO. 2015. Report of the 8th Meeting of the NAFO Scientific Council (SC) Working Group on Ecosystem Science and Assessment (WGESA). 17-26 November 2015, Dartmouth, Canada. NAFO SCS Doc. 15/19.Pepin, P., Higdon, J., Koen-Alonso, M., Fogarty, M., and N. Ollerhead. 2014. Application of ecoregion analysis to the identification of Ecosystem Production Units (EPUs) in the NAFO Convention Area. NAFO SCR Doc. 14/069.

Three marine spatial planning areas are delineated in Eastern Canada to define the spatial extents of marine spatial plans being led by Fisheries and Oceans Canada (DFO): the Estuary and Gulf of St. Lawrence (EGSL), the Newfoundland and Labrador (NL) Shelves, and the Scotian Shelf and Bay of Fundy. The EGSL planning area includes the St. Lawrence River estuary from northeast of Île d’Orléans, Quebec, the Saguenay River estuary, and the entire Gulf of St. Lawrence as far north as the Strait of Belle Isle (NAFO Divisions 4RST). The NL Shelves planning area includes areas off southern, eastern and northern Newfoundland, part of the Churchill River and Lake Melville, as well as off the Labrador coast to the extent of the exclusive economic zone (EEZ) (NAFO Divisions 2GHJ and 3KLNOP). The Scotian Shelf and Bay of Fundy planning area includes DFO Maritimes’ administrative region off the Atlantic coast of Nova Scotia to the extent of the EEZ, the Bay of Fundy and the Canadian portion of the Gulf of Maine (NAFO Divisions 4VWX, 5Ze, and the Canadian portion of 5Y). The French EEZ for St. Pierre et Miquelon is excluded from the three planning areas. These planning areas are derived from Federal Marine Bioregions (https://open.canada.ca/data/en/dataset/23eb8b56-dac8-4efc-be7c-b8fa11ba62e9) that were developed by a Canadian Science Advisory Secretariat process using ecosystem-based management principles to define 13 ecological bioregions that have informed but not directed DFO implementation of marine spatial planning.

In 2016-17, DFO Maritimes Region undertook a Marine Protected Area (MPA) network analysis for the Scotian Shelf-Bay of Fundy Bioregion. The analysis considered available bioregional-scale ecological and human use data in an effort to identify a draft MPA network design that would protect biodiversity while minimizing any potential impacts on commercial fishing and other industries. The data layers used for the offshore component of the MPA network analysis are provided here. These layers are not presented in their original forms and were modified (e.g. clipped, reclassified, etc.) specifically for use in the MPA network analysis. They should not be used for any other purpose. Please see Serdynska et al. 2021 for details on how each layer was created.Serdynska, A.R., Pardy, G.S., and King, M.C. 2021. Offshore Ecological and Human Use Information considered in Marine Protected Area Network Design in the Scotian Shelf Bioregion. Can. Tech. Rep. Fish. Aquat. Sci. 3382: xi + 100 p. https://publications.gc.ca/collections/collection_2021/mpo-dfo/Fs97-6-3382-eng.pdfCite this data as: Serdynska, A.R., Pardy, G.S., and King, M.C. Data of: Offshore Ecological and Human Use Information considered in Marine Protected Area Network Design in the Scotian Shelf Bioregion. Published: January 2022. Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/2d9cce9a-d634-4b49-879f-87c40c52acf2

Identification of significant concentrations of sea pens in the Gulf of St. Lawrence biogeographic unit using Kernel density estimation (KDE).This method was applied to create a modelled biomass surface for each taxa and an aerial expansion method was permitted to identify significant concentrations. Only geo-referenced biomass data have been used to identify the “hot spots”. The borders of the areas were refined using knowledge of null catches and species distribution models. Predictive models were produced using a random forest machine-learning technique. For more details, please refer to this report: Kenchington, E., L. Beazley, C. Lirette, F.J. Murillo, J. Guijarro, V. Wareham, K. Gilkinson, M. Koen Alonso, H. Benoît, H. Bourdages, B. Sainte-Marie, M. Treble, and T. Siferd. 2016. Delineation of Coral and Sponge Significant Benthic Areas in Eastern Canada Using Kernel Density Analyses and Species Distribution Models. DFO Can. Sci. Advis. Sec. Res. Doc. 2016/093. vi + 178 p.http://waves-vagues.dfo-mpo.gc.ca/Library/40577806.pdfThe present layer only contains the analysis results for sea pens. Purpose:As part of the Canada's commitment to the identification and protection of sensitive benthic marine ecosystems, maps of the location of significant concentrations of corals and sponges on the east coast of Canada were produced through quantitative analyses of research vessel trawl survey data, supplemented with other data sources where available. The taxa analyzed are sponges (Porifera), large and small gorgonian corals (Alcyonacea), and sea pens (Pennatulacea). However, only the sponges (Porifera) and sea pens (Pennatulacea) have been considered in the analysis concerning the Gulf of St. Lawrence biogeographic unit.

A relative exposure index (REI), unweighted fetch, effective fetch, and other fetch-based indices (i.e., sum, minimum) were calculated for the Newfoundland and Labrador (NL) Shelves bioregion. Due to the extensive coastline of the study region, this analysis was conducted for a 5km buffered region along the coast at a spatial resolution of 250m. Detailed methods on the selection of input points for the NL bioregion are included below.MethodsPreprocessing and input point selection:Land boundary files were obtained for Eastern Canada and the Canadian Arctic (NrCan 2017) at a scale of 1:50,000 as well as for Saint Pierre and Miquelon (Hijmans 2015), and the New England states (GADM 2012) however the scale at which these layers were produced is unknown. Land boundary files were merged into a single land polygon layer and watercourses reaching for in-land and/or above sea level were clipped from this polygon layer (Greyson 2021). A 5km buffer was generated around the NL provincial boundary. This buffer was then clipped by all land polygons to remove areas overlapping land polygons within the study area. All buffer segments intersecting the NAFO divisions within the NL bioregion were selected and the Union tool in ArcGIS Pro (v. 2.7.2) was used to fill-in gaps within the buffered area, creating a more continuous polygon. The buffered layer was then dissolved, and the NL provincial boundary polygon was erased from the buffered layer to create the study area polygon. A 250m fishnet was created and clipped to the study area (5km buffer layer) and the feature to point tool was used (with the “inside parameter checked”) to convert this grid into a point layer (approx. 1,000,000 points). The spatial resolution for all subsequent analyses was matched to the fishnet grid at 250m.ReferencesGADM database of Global Administrative Areas (2012). Global Administrative Areas, version 2.0. (accessed 2 December 2020). www.gadm.orgGreyson, P (2021) Land boundary file for Eastern Canada, the Canadian Arctic, the New England States and Saint Pierre and Miquelon. [shapefile]. Unpublished data.Hijmans, R. and University of California, Berkeley, Museum of Vertebrate Zoology. (2015). First-level Administrative Divisions, Saint Pierre and Miquelon, 2015. UC Berkeley, Museum of Vertebrate Zoology. Available at: http://purl.stanford.edu/bz573nv9230Natural Resources Canada (2017) Administrative Boundaries in Canada - CanVec Series - Administrative Features - Open Government Portal. (accessed 2 December 2020). https://open.canada.ca/data/en/dataset/306e5004-534b-4110-9feb-58e3a5c3fd97.

Launched in 2017, the Cumulative Effects of Marine Shipping (CEMS) initiative is part of Canada’s $1.5 billion Oceans Protection Plan, which is providing economic opportunities to Canadians today, while protecting our coasts and waterways for future generations. The Cumulative Effects of Marine Shipping initiative is another way that the Government of Canada is protecting our coasts and waterways.https://tc.canada.ca/en/marine-transportation/marine-pollution-environmental-response/cumulative-effects-marine-shippingAs part of this initiative, Transport Canada is working with Indigenous partners and stakeholders in six pilot areas across Canada. Together, we are trying to understand the effects of marine shipping in various coastal areas. These pilot areas include:- North Coast British Columbia- South Coast British Columbia- St. Lawrence and Saguenay Rivers, Quebec- Bay of Fundy, New Brunswick and Nova Scotia- Placentia Bay, Newfoundland- Cambridge Bay, Nunavut

The Oceans Act (1997) commits Canada to maintaining biological diversity and productivity in the marine environment. A key component of this is to identify areas that are considered ecologically or biologically significant. Fisheries and Oceans Canada (DFO) Science has developed guidance on the identification of Ecologically or Biologically Significant Areas (EBSAs) (DFO 2004) and has endorsed the scientific criteria of the Convention on Biological Diversity (CBD) for identifying ecologically or biologically significant marine areas as defined in Annex I of Decision IX/20 of its 9th Conference of Parties. These criteria were applied to the Newfoundland and Labrador (NL) Shelves Bioregion in two separate data-driven processes. The first process focused on the area north of the Placentia Bay-Grand Banks (PBGB) Large Ocean Management Area (LOMA) (DFO 2013). The second process focused on the PBGB area (DFO 2019), where EBSAs had previously been identified using a more Delphic approach (Templeman 2007). In both cases, an EBSA Steering Committee, comprised of experts in oceanography, ecosystem structure and function, taxa-specific life histories and Geographic Information Systems (GIS) guided the process by advising or aiding in the identification, collection, processing and analysis of data layers, as well as participating in the final selection of candidate EBSAs (Wells et al. 2017, Ollerhead et al. 2017, Wells et al. 2019). All information was compiled in a GIS and a hierarchical approach was used to review individual data layers and groupings of data layers. Peer review meetings were held for both processes, during which candidate EBSAs were reviewed and the final EBSAs were agreed upon and delineated. In the northern study area, a total of fifteen EBSAs were identified and described; three of these areas are primarily coastal areas; seven are in offshore areas; four EBSAs straddle coastal and offshore areas; and one is a transitory EBSA that follows the southern extent of pack ice. In the PBGB study area, fourteen EBSAs were identified in two different categories: seven based on coastal data and seven based on offshore data. In comparing the new PBGB EBSAs to those identified in 2007, nine of them overlap spatially and are based on similar features; however, there were some variations in the boundaries. Two of the EBSAs that were identified in 2007 were no longer considered EBSAs in 2017, but portions of both of these areas were captured in part by other EBSAs. Five new EBSAs were identified in areas not previously considered.References:DFO, 2004. Identification of Ecologically and Biologically Significant Areas. DFO Can. Sci. Advis. Sec. Ecosystem Status Rep. 2004/006.DFO. 2013. Identification of additional Ecologically and Biologically Significant Areas (EBSAs) within the Newfoundland and Labrador Shelves Bioregion. DFO Can. Sci. Advis. Sec. Sci. Advis. Rep. 2013/048.DFO. 2019. Re-evaluation of the Placentia Bay-Grand Banks Area to Identify Ecologically and Biologically Significant Areas . DFO Can. Sci. Advis. Sec. Sci. Advis. Rep. 2019/040.Ollerhead, L.M.N., Gullage, M., Trip, N., and Wells, N. 2017. Development of Spatially Referenced Data Layers for Use in the Identification and Delineation of Candidate Ecologically and Biologically Significant Areas in the Newfoundland and Labrador Shelves Bioregion. DFO Can. Sci. Advis. Sec. Res. Doc. 2017/036. v + 38 pTempleman, N.D. 2007. Placentia Bay-Grand Banks Large Ocean Management Area Ecologically and Biologically Significant Areas. Can. Sci. Advis. Sec. Res. Doc. 2007/052: iii + 15 p.Wells, N.J., Stenson, G.B., Pepin, P., and Koen-Alonso, M. 2017. Identification and Descriptions of Ecologically and Biologically Significant Areas in the Newfoundland and Labrador Shelves Bioregion. DFO Can. Sci. Advis. Sec. Res. Doc. 2017/013. v + 87 p.Wells, N., K. Tucker, K. Allard, M. Warren, S. Olson, L. Gullage, C. Pretty, V. Sutton-Pande and K. Clarke. 2019. Re-evaluation of the Placentia Bay-Grand Banks Area of the Newfoundland and Labrador Shelves Bioregion to Identify and Describe Ecologically and Biologically Significant Areas. DFO Can. Sci. Advis. Sec. Res. Doc. 2019/049. viii + 138 p.

An ecoregion is an area with major physiographic and minor macroclimatic or oceanographic variation. There are 43 ecoregion in British Columbia of which 39 are terrestrial. Ecodivsions are meant to be mapped at 1:500,000 for regional strategic planning