A research survey on American lobster (Homarus americanus) and rock crab (Cancer irroratus) has been carried out annually on the Magdalen Islands since 1995 to assess the abundance and structure of populations in this area. The survey is carried out during the first two weeks of September and covers the southern part of the Magdalen Islands, from "Baie de Plaisance" to "Grande-Entrée", extending slightly eastwards between "Havre-aux-Maisons" and "Grande-Entrée". The survey lasts 8 to 15 days, depending of the weather conditions, and covers a maximum of 48 stations. Approximately half of the stations are surveyed in duplicate (two tows per station). The tows always cover 500 meters, with a slightly variable trawl opening (as explained above), averaging around 10 meters.The gear used is a Nephrops trawl, a benthic trawl originally developed for langoustine fishing in Brittany, but slightly modified to target lobster. The trawl is deployed from the CCGS Leim and the harvested organisms are hauled aboard and sorted. Cephalothorax length, sex and shell stage are recorded for all lobsters. This is supplemented by information on reproductive conditions for certain size classes, and dissections of 25 males and 25 females per mission. Rock crabs are also included in the survey and are counted with additional measurements of shell width, sex and shell condition that are recorded on predetermined stations. For other stations individuals data are collected depending on the time available between trawl hauls.The publication contains 4 files; the "Information_stations" file which contains the data of the stations, the "Data_homard_lobster_survey" file which contains the measurements of each lobster, the "Nombre_crabe_crab_number" file which contains the count of rock crabs, the "Mesure_crabe_crab_measurement" file contains the characteristics measured for each crab. Each of the files can be linked by the "date", "st", "tr" columns. The “bat” column notes the boat code, because the gear is not deployed in the same way on each boat.*This record consist of raw data and quality has not been verifiedAssociated species are identified and semi-quantitatively counted directly on the sorting table, and the results are presented in the following publication: https://open.canada.ca/data/en/dataset/99cb7034-f3d5-4da4-a5bc-e81315cfb8eb

This data report provides information on temperature and salinity in the Godbout region of the St. Lawrence Estuary. Sampling was carried out from 2019 to 2021 over an area of <5 km2. The databases provide information on temperature and salinity at an hourly rate for 2 years. The aim of this project is to analyze telemetry data from sea urchins (Strongylocentrotus droebachiensis), snow crabs (Chionoecetes opilio), rock crabs (Cancer irroratus), spider crabs (Hyas spp.) and whelks (Buccinum undatum). This report focuses on the presentation of benthic environmental data collected throughout the study with high spatial and temporal resolution. All reported variables were collected at the seafloor, as the aim of the project was to study the movement of epibenthic species. Temperature data were collected from three devices: telemetry receivers with integrated temperature sensors (InnovaseaTM), HoboTM and Star-OddiTM probes. Temperature data processing involved cleaning up extreme values (below 2°C and above 20°C) and homogenizing the data to fit the bathymetry matrix (1m x 1m cells) of the study site. Temperature data are provided in a NetCDF file with a matrix of the entire study site, where there is a stratum for each hour between August 2019 and October 2021 and in each file, a temperature value for each pixel of the raster. Salinity data were collected from Star-OddiTM probes only. Salinity values were averaged hourly for the entire study area. Salinity data is provided as a CSV file with one salinity value per hour for the entire study area.

SummaryThe Quebec region of the Department of Fisheries and Oceans (DFO) is responsible for the assessment of several fish and invertebrate stocks exploited in the Estuary and the northern Gulf of St. Lawrence. The commercial catches sampling program is one of the sources of information used to complete these assessments. The data collected by this program, at wharf or at sea, offers among other things the advantage of a relatively large spatio-temporal coverage and provides some of the necessary knowledge to assess the demography and the structure of the exploited populations. This program is implemented by specialized DFO staff whose main mandate is to collect biological data on groundfish, pelagic fish and marine invertebrate species that are commercially exploited in the various marine communities.DataThis dataset on the common rock crab (Cancer irroratus) includes the metadata, sample weight, shell width, shell condition and the sex of the specimens measured. This dataset covers the periods of 1990 and 1995 to present. In order to protect the confidentiality of the sources, some informations (such as those concerning the vessel) have been excluded and others (such as the date of capture) have been simplified. Entries where there was only one vessel in a fishing area for a given year were also excluded. Further information including the fishing areas coordinates can be found by clicking on the «Atlantic and Arctic commercial fisheries» and «Fishing areas» links below.

This data set provides geographic information for Health PEI facilities (Hospitals, Cancer Treatment Centres, Palliative Care Centre, Primary Care Networks, and Public Nursing Homes.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

An exploratory project on the taxonomic and genetic diversity of decapods in three ocean subregions (Northeast Pacific, Canadian Arctic, and Northwest Atlantic), which were sampled in 2022, was undertaken by the Arctic Working Group under the Canada-U.S. Fisheries and Climate Collaboration between Fisheries and Oceans Canada (DFO) and the National Marine Fisheries Service (NMFS) of the National Oceanic and Atmospheric Administration (NOAA). This collaboration framework aims to pool Canadian and U.S. data to explore the impacts of broad-scale climate change on marine biodiversity. In early summer 2022, a sampling protocol with the selection of targeted decapods was provided to DFO and NOAA collaborators. Targeted genera were collected from a total of 10 research programs across three ocean subregions and four marine regions. The Northeast Pacific samples were collected in the Bering Sea during the Northern Bering Sea Ecosystem and Surface Trawl Survey, and the Eastern and Northern Bering Sea Continental Shelf Bottom Trawl Survey of Groundfish and Invertebrate Fauna onboard the F/V Northwest Explorer, F/V Alaska Knight and F/V Vesteraalen. In the Western Canadian Arctic (mainly from Beaufort Sea and Amundsen Gulf), specimens were collected during DFO’s Canadian Beaufort Sea – Marine Ecosystem Assessment (CBS-MEA) survey onboard the F/V Frosti. In Eastern Canadian Arctic (mainly from Baffin Bay and Davis Strait), specimens were collected during DFO’s Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) survey onboard the CCGS Amundsen and DFO’s North Atlantic Fisheries Organization (NAFO) Subarea 0B survey onboard the R/V Tarajoq. In the Estuary and Gulf of St. Lawrence (EGSL), specimens were collected from coastal surveys (scallops, sea cucumber, snow crab, and whelk surveys) onboard the CCGS Leim and offshore during the Ecosystemic Survey onboard the CCGS Teleost. Decapods were collected from various sampling gears (benthic beam trawl, modified Atlantic Western IIA otter trawl, Bacalao trawl, shrimp trawl, Digby scallop dredge, or modified sea cucumber dredge) and identified to the lowest possible taxonomic level and photographed, when possible. All specimens were frozen at sea (n = 995). In the lab, the identifications were validated or refined with the photos and the frozen specimens. DNA was extracted for 87 specimens and a section of COI gene was amplified in order to be sequenced using Sanger method. Sequences were compared with existing data using The Basic Local Alignment Search Tool (BLAST) in the National Center for Bio-technology Information Nucleotide database (NCBI-nt, including the GenBank database) to compare scientific names, where available.The present dataset includes 391 decapod species occurrences. DNA was extracted for a subset of 87 specimens (COI gene); sequences are publicly available on BOLD data portal under project code DDAO (see supporting document "citations_references.csv" for more information).The data are presented in Darwin Core format and are separated in three files:The "Activité_décapodes_DDAO_decapods_event_en" file contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The "Occurrence_décapodes_DDAO_decapods_en" file contains the taxonomic occurrences.The "ADN_décapodes_DDAO_decapods_DNA_en" file contains the DNA derived data.For further details, please refer to the technical report available in the supporting document named "citations_references.csv". USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Historical finds of Profenusa thomsoni

CMIST is a peer-reviewed screening-level risk assessment tool for marine invasive species (Drolet et al. 2016). It is a short questionnaire that follows the invasion process from arrival to impact and is designed so an informed assessor can evaluate one species in an assessment area in approximately one day using easily accessible information from internet databases, primary literature, and grey literature. Species can be those with an invasion history in an area or those that are candidates for future invasions.CMIST is score-based and incorporates both likelihood and impact of invasion as well as uncertainty. Questions are general to make CMIST broadly applicable to different taxa, different assessment areas, and different project goals. To date, CMIST has been tested with molluscs, tunicates, crustaceans, and polychaetes introduced or at risk of introduction to three Canadian marine ecoregions (DFO 2009). CMIST has also been successfully applied to non-indigenous freshwater fish in British Columbia with adapted guidelines (T. Therriault, pers. comm.). Upon completion, CMIST produces a risk score adjusted for the assessor’s uncertainty which, combined with information collected during the assessment, can be used to assist in management decisions. For example, in 2015, CMIST assessments were used to identify high risk invaders in three Canadian marine ecoregions (DFO 2016).

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

This dataset represents the radioisoptopic tracers for selected rocks in the province of Saskatchewan, Canada.This dataset represents the compilation of radioisoptopic tracers for selected rocks in the province of Saskatchewan, Canada.  **Please Note – All published Saskatchewan Geological Survey datasets, including those available through the Saskatchewan Mining and Petroleum GeoAtlas, are sourced from the Enterprise GIS Data Warehouse. They are therefore identical and share the same refresh schedule.