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We have found 10 datasets for the keyword "genbank". You can continue exploring the search results in the list below.
Datasets: 105,254
Contributors: 42
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10 Datasets, Page 1 of 1
Cold-water coral DNA sequences from Eastern Canada: Part 1
Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.
Manitoba COVID-19 Rapid Antigen Test Kit Distributors
Feature point layer showing COVID-19 rapid antigen test kit distribution sites in Manitoba.Feature point layer showing locations of distribution sites that currently have COVID-19 rapid antigen test kits available in Manitoba. The main purpose of this data is to provide the public with the hours of operation for these sites. Distributors include pharmacies, retail stores, and community libraries. This data is populated by Manitoba Shared Health and is updated as needed. This feature layer is used in the Manitoba COVID-19 Rapid Antigen Test Kit Availability Map and Manitoba COVID-19 Rapid Antigen Test Kit Availability application. For more information on Manitoba’s response to COVID-19, please visit the following site: https://www.gov.mb.ca/covid19/index.html Fields included [Alias (Field Name): Field description] Site Category (Site_Category): Indicates whether the site is a retail business, pharmacy, or community library Site Name (Site_Name): Official business name of the site Address (Address): Street address of the site City or Town (City_or_Town): City or town in which the site is located Postal Code (Postal_Code): Postal code that corresponds with the street address of the site Phone (Phone): Telephone number for the site French Service (French_Service): Indicates whether a site self-identified as having services available in French; if unknown, value is null Health Authority (Health_Authority): Name of the regional health authority where the site is located Hours of Operation (Hours_of_Operation): Listing of days and times that the site is open to the public Alt Hours Start (Alt_Hours_Start): Start date of when alternate hours are in effect Alt Hours End (Alt_Hours_End): End date of when alternate hours are in effect Alternate Hours of Operation (Alternate_Hours_of_Operation): Listing of alternate days and times that the site is open to the public Latitude (Latitude): Latitudinal coordinate of the site Longitude (Longitude): Longitudinal coordinate of the site
The Canadian Radiological Monitoring Network – Gross Alpha / Beta in Drinking Water
This dataset provides the results obtained by Health Canada’s Canadian Radiological Monitoring Network (CRMN) for the gross alpha and beta activity concentrations in drinking water, given in units of becquerels per liter (Bq/L). More information about the CRMN network can be found on the Health Canada website (see link below). Although water quality is a matter of provincial jurisdiction, the CRMN, in collaboration with the city of Ottawa, has been conducting a targeted program to monitor the radiological content of drinking water from two water treatment plants in Ottawa, ON. The Guidelines for Canadian Drinking Water Quality recommend screening levels of 0.5 Bq/L and 1.0 Bq/L for gross alpha and gross beta activity, respectively. The screening levels are set to reflect the most restrictive Maximum Acceptable Concentrations (MACs) for specific radionuclides in drinking water. If the screening levels are not exceeded, compliance with the guidelines can be inferred. The screening levels set out in the Guidelines for Canadian Drinking Water Quality are calculated based on annual averages of radionuclides in drinking water. Short-term exposure to levels above those recommended by these guidelines does not indicate a health risk. The measured gross alpha and gross beta activity concentrations presented here are well below the screening levels set by the Guidelines for Canadian Drinking Water Quality, with only one exception to date. This occurred February 28, 2011, and was attributable to the flushing of lead pipes at the water treatment plant. It resulted in a spike of naturally occurring lead radionuclides that was dealt with immediately by the City of Ottawa. The map shows the approximate sampling location for each monitoring station. Stations are found within the associated location range.
Manitoba Hog Prices 10 years
Manitoba market hog prices and United States (U.S.) iso-wean pig and feeder pig prices weekly and monthly, for the last 10 years.The table represents prices paid for market hogs in Manitoba and iso-wean and feeder pigs in the U.S. weekly and monthly for the current and last 10 years. For hog price report definitions and calculations, click here. Manitoba market hog prices are collected from major processors in Manitoba, compiled and released weekly by Manitoba Agriculture and Resource Development (ARD). Manitoba market hog prices are weighted by the volume of hogs processed, and averaged monthly. United States (U.S.) iso-wean and feeder pig prices are sourced from the United States Department of Agriculture (USDA) and presented in Canadian dollars (C$) using the Bank of Canada exchange rate. Monthly U.S. iso-wean and feeder pig prices are a simple average of the weekly U.S. total composite weighted average prices. Fields included (Alias (Field name): Field description) Year (Year): year from the selection of last ten years including current year Period (Period): period of time to be presented on charts from the selection of Monthly and Weekly PeriodNo (PeriodNo): serial number of period (1-12 for Monthly presentation, 1-52 for Weekly presentation) Price (Price): Hogs price for the corresponding period of time (C$ per head for U.S. feeder pigs and iso-wean pigs, CAD per 100 kg for Manitoba market hogs)Hog category (Hog category): category of animals from the selection of U.S. feeder pigs, U.S. iso-wean pigs, Manitoba market hogs
MetNotes
MetNotes are a geo- and time-referenced, free form polygon product issued by MSC that complement today's location-based dissemination systems. The concise text of a MetNote (similar to a Tweet) is consistent with communication today where people are seeking information at a glance. Meteorologists will issue a MetNote to add contextual and/or impact information to complement the public forecast that is valid over a specific area, for a specific time range.
YEC Power substations
Yukon Energy Corporation (YEC) Power S ubstations are the locations where electrical power is transformed, generally from high to low voltage. This data was provided by YEC and will be updated when new substations are constructed.Distributed from [GeoYukon](https://yukon.ca/geoyukon) by the [Government of Yukon](https://yukon.ca/maps) . Discover more digital map data and interactive maps from Yukon's digital map data collection.For more information: [geomatics.help@yukon.ca](mailto:geomatics.help@yukon.ca)
The Canadian Radiological Monitoring Network – Strontium-90 in Milk
This dataset provides results obtained by Health Canada’s Canadian Radiological Monitoring Network (CRMN) for 90Sr activity in milk samples originating from various locations across Canada. More information about the CRMN network can be found on the Health Canada website (see link below). From 1984 to 1993, data was collected from 20 locations. After 1993, routine milk analysis for radio-strontium activity was discontinued for all monitoring stations, except the Ottawa location. The results provided here are 90Sr activity concentrations in units of becquerels per litre (Bq/L). Milk is an important matrix for environmental radioactivity assessment since many radionuclides of health concern exhibit efficient soil to milk uptake pathways. As such, the surveillance of milk provides a very good indication as to the severity of environmental impact following a nuclear event. Strontium-90 (90Sr) is a radionuclide of particular concern because it has a long radiological and biological half-life coupled with a high affinity for incorporation into human bone and teeth. Nuclear weapons testing and nuclear accidents have released such long-lived radionuclides into the atmosphere, which in turn have entered the biological life cycle. Nevertheless, a steady decline in the activity concentration of 90Sr in milk can be seen since most of the nuclear weapon testing was halted in the 1960s. The map shows the approximate sampling location for each monitoring station. Stations are found within the associated location range.
Taxonomic and Genetic Diversity of Decapods in Northeast Pacific, Canadian Arctic and Northwest Atlantic
An exploratory project on the taxonomic and genetic diversity of decapods in three ocean subregions (Northeast Pacific, Canadian Arctic, and Northwest Atlantic), which were sampled in 2022, was undertaken by the Arctic Working Group under the Canada-U.S. Fisheries and Climate Collaboration between Fisheries and Oceans Canada (DFO) and the National Marine Fisheries Service (NMFS) of the National Oceanic and Atmospheric Administration (NOAA). This collaboration framework aims to pool Canadian and U.S. data to explore the impacts of broad-scale climate change on marine biodiversity. In early summer 2022, a sampling protocol with the selection of targeted decapods was provided to DFO and NOAA collaborators. Targeted genera were collected from a total of 10 research programs across three ocean subregions and four marine regions. The Northeast Pacific samples were collected in the Bering Sea during the Northern Bering Sea Ecosystem and Surface Trawl Survey, and the Eastern and Northern Bering Sea Continental Shelf Bottom Trawl Survey of Groundfish and Invertebrate Fauna onboard the F/V Northwest Explorer, F/V Alaska Knight and F/V Vesteraalen. In the Western Canadian Arctic (mainly from Beaufort Sea and Amundsen Gulf), specimens were collected during DFO’s Canadian Beaufort Sea – Marine Ecosystem Assessment (CBS-MEA) survey onboard the F/V Frosti. In Eastern Canadian Arctic (mainly from Baffin Bay and Davis Strait), specimens were collected during DFO’s Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) survey onboard the CCGS Amundsen and DFO’s North Atlantic Fisheries Organization (NAFO) Subarea 0B survey onboard the R/V Tarajoq. In the Estuary and Gulf of St. Lawrence (EGSL), specimens were collected from coastal surveys (scallops, sea cucumber, snow crab, and whelk surveys) onboard the CCGS Leim and offshore during the Ecosystemic Survey onboard the CCGS Teleost. Decapods were collected from various sampling gears (benthic beam trawl, modified Atlantic Western IIA otter trawl, Bacalao trawl, shrimp trawl, Digby scallop dredge, or modified sea cucumber dredge) and identified to the lowest possible taxonomic level and photographed, when possible. All specimens were frozen at sea (n = 995). In the lab, the identifications were validated or refined with the photos and the frozen specimens. DNA was extracted for 87 specimens and a section of COI gene was amplified in order to be sequenced using Sanger method. Sequences were compared with existing data using The Basic Local Alignment Search Tool (BLAST) in the National Center for Bio-technology Information Nucleotide database (NCBI-nt, including the GenBank database) to compare scientific names, where available.The present dataset includes 391 decapod species occurrences. DNA was extracted for a subset of 87 specimens (COI gene); sequences are publicly available on BOLD data portal under project code DDAO (see supporting document "citations_references.csv" for more information).The data are presented in Darwin Core format and are separated in three files:The "Activité_décapodes_DDAO_decapods_event_en" file contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The "Occurrence_décapodes_DDAO_decapods_en" file contains the taxonomic occurrences.The "ADN_décapodes_DDAO_decapods_DNA_en" file contains the DNA derived data.For further details, please refer to the technical report available in the supporting document named "citations_references.csv". USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.
Licensed Payday Lenders in Nova Scotia
List of businesses located in Nova Scotia, licensed to provide payday loans
Record - S1A-EW-GRDM-1SDH-20231220T184607-20231220T184640-051742-063FD1-FDA4-Sentinel-1
The Sentinel mirror is maintained by the Government of Canada through the Copernicus collaborative ground segment program as well as EUMETSAT. Data is made available as quickly as possible based on Canada coverage availability at the source. **This third party metadata element follows the Spatio Temporal Asset Catalog (STAC) specification.**
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