Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Food Banks is a point dataset identifying food banks in British Columbia.

Feature point layer showing COVID-19 rapid antigen test kit distribution sites in Manitoba.Feature point layer showing locations of distribution sites that currently have COVID-19 rapid antigen test kits available in Manitoba. The main purpose of this data is to provide the public with the hours of operation for these sites. Distributors include pharmacies, retail stores, and community libraries. This data is populated by Manitoba Shared Health and is updated as needed. This feature layer is used in the Manitoba COVID-19 Rapid Antigen Test Kit Availability Map and Manitoba COVID-19 Rapid Antigen Test Kit Availability application. For more information on Manitoba’s response to COVID-19, please visit the following site: https://www.gov.mb.ca/covid19/index.html Fields included [Alias (Field Name): Field description] Site Category (Site_Category): Indicates whether the site is a retail business, pharmacy, or community library Site Name (Site_Name): Official business name of the site  Address (Address): Street address of the site  City or Town (City_or_Town): City or town in which the site is located Postal Code (Postal_Code): Postal code that corresponds with the street address of the site  Phone (Phone): Telephone number for the site French Service (French_Service): Indicates whether a site self-identified as having services available in French; if unknown, value is null   Health Authority (Health_Authority): Name of the regional health authority where the site is located Hours of Operation (Hours_of_Operation): Listing of days and times that the site is open to the public Alt Hours Start (Alt_Hours_Start): Start date of when alternate hours are in effect Alt Hours End (Alt_Hours_End): End date of when alternate hours are in effect Alternate Hours of Operation (Alternate_Hours_of_Operation): Listing of alternate days and times that the site is open to the public Latitude (Latitude): Latitudinal coordinate of the site Longitude (Longitude): Longitudinal coordinate of the site

This dataset provides the results obtained by Health Canada’s Canadian Radiological Monitoring Network (CRMN) for the gross alpha and beta activity concentrations in drinking water, given in units of becquerels per liter (Bq/L). More information about the CRMN network can be found on the Health Canada website (see link below). Although water quality is a matter of provincial jurisdiction, the CRMN, in collaboration with the city of Ottawa, has been conducting a targeted program to monitor the radiological content of drinking water from two water treatment plants in Ottawa, ON. The Guidelines for Canadian Drinking Water Quality recommend screening levels of 0.5 Bq/L and 1.0 Bq/L for gross alpha and gross beta activity, respectively. The screening levels are set to reflect the most restrictive Maximum Acceptable Concentrations (MACs) for specific radionuclides in drinking water. If the screening levels are not exceeded, compliance with the guidelines can be inferred. The screening levels set out in the Guidelines for Canadian Drinking Water Quality are calculated based on annual averages of radionuclides in drinking water. Short-term exposure to levels above those recommended by these guidelines does not indicate a health risk. The measured gross alpha and gross beta activity concentrations presented here are well below the screening levels set by the Guidelines for Canadian Drinking Water Quality, with only one exception to date. This occurred February 28, 2011, and was attributable to the flushing of lead pipes at the water treatment plant. It resulted in a spike of naturally occurring lead radionuclides that was dealt with immediately by the City of Ottawa. The map shows the approximate sampling location for each monitoring station. Stations are found within the associated location range.

A Sign is a lettered board, message or other display which includes all regulatory, warning, guide, informational, advisory, construction and maintenance and route markers, but excluding electronically controlled messages/displays. It is a Point feature

This dataset provides results obtained by Health Canada’s Canadian Radiological Monitoring Network (CRMN) for 90Sr activity in milk samples originating from various locations across Canada. More information about the CRMN network can be found on the Health Canada website (see link below). From 1984 to 1993, data was collected from 20 locations. After 1993, routine milk analysis for radio-strontium activity was discontinued for all monitoring stations, except the Ottawa location. The results provided here are 90Sr activity concentrations in units of becquerels per litre (Bq/L). Milk is an important matrix for environmental radioactivity assessment since many radionuclides of health concern exhibit efficient soil to milk uptake pathways. As such, the surveillance of milk provides a very good indication as to the severity of environmental impact following a nuclear event. Strontium-90 (90Sr) is a radionuclide of particular concern because it has a long radiological and biological half-life coupled with a high affinity for incorporation into human bone and teeth. Nuclear weapons testing and nuclear accidents have released such long-lived radionuclides into the atmosphere, which in turn have entered the biological life cycle. Nevertheless, a steady decline in the activity concentration of 90Sr in milk can be seen since most of the nuclear weapon testing was halted in the 1960s. The map shows the approximate sampling location for each monitoring station. Stations are found within the associated location range.

Yukon Energy Corporation (YEC) Power S ubstations are the locations where electrical power is transformed, generally from high to low voltage. This data was provided by YEC and will be updated when new substations are constructed.Distributed from [GeoYukon](https://yukon.ca/geoyukon) by the [Government of Yukon](https://yukon.ca/maps) . Discover more digital map data and interactive maps from Yukon's digital map data collection.For more information: [geomatics.help@yukon.ca](mailto:geomatics.help@yukon.ca)

The spatial representation for a Public Sustained Yield Unit, which is an area of Crown land, usually a natural topographic unit determined by drainage areas, managed for sustained yield by the Crown through the Ministry of Forests. It includes all Crown lands within the currently established boundaries of the unit and excludes federal lands, provincial parks, experimental forest reserves, gazetted watersheds and tree farm licences. Crown land designated as a public sustained yield unit under Section 6 of the Forest Act. A portion of a TSA

A vector representation of stream networks is a crucial dataset for the modelling the surface water and groundwater components of the hydrologic cycle. For many usages a crucial attribute of the drainage network is a digital topology and hierarchal stream order attribute (e.g., Strahler stream order). In Canada jurisdictional stream networks are available for the provinces and territories and nationally for Canada in the National Hydrological Network (NHN) dataset. Unfortunately, the NHN data lacks the same topological and attribute information that is available for numerous provinces due to standardization for the entire country. For Canada1Water it was also necessary to have a harmonized dataset with the United States, for both the southern transboundary watersheds and the Alaskan watersheds. This report documents the processes completed to upgrade the topological and graph network support for NHN and provide continuous connectivity with US datasets. It also highlights and corrects a number of stream density and stream order issues that occur within Canada across provincial and territorial borders and NTS tiles. All vector processing was completed in RivEX software extension for ArcMap. Following complete topological correction stream classification was assigned and a table of the node graph network developed. Additional work was then completed to normalize stream density particularly amongst low-order streams between British Columbia and the Yukon and amongst local NTS tiles in Quebec and Ontario. Corrected NHN Strahler stream order assignment was validated against a number of provincial and watershed datasets, all of which already have Strahler stream order attributed. These datasets are the same underlying digitized vector data, so there are no differences in node or polyline positions. Strahler stream order assignment validation was only done by visual comparison as due to differences in vector segments a statistical comparison is complicated. The transboundary integrated C1W stream network with complete classification provides a seamless national dataset to support transdisciplinary studies (fisheries, wildlife, health, pesticide and nutrient issues, mining impact, ecosystem restoration, numeric modelling) that involve a knowledge of stream distribution and ranking.

The Base Hydrography Single Line Hydrography Network (SLNET) is the Alberta Environment and Protected Areas base SLNET data set. It is an update of the Base Features HydroSLNET in Southern Alberta from orthophoto and various sources of provincial base data. It contains all captured single line representations of hydrographic features. In addition, single line representations of polygonal features and single line arbitrary network connectors are in the file.