Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

BiologicEcologic ISO Feature Dataset symbolization and publication. September 5, 2017.

This dataset is a collaborative project by Natural Resources Canada and the federal, provincial and territorial members of the Geographical Names Board of Canada, illustrating a curated selection of official geographical names in Canada that have changed over time. The selection comes from the Canadian Geographical Names Database, displaying additional information including the previous name(s) of each feature, the year and the reason why the names changed along with a short history of each name change.

This spatial data identifies breeding zones used by forest managers and forest genetic associations to manage provincial forest genetic assets. The data: * shows the boundaries of breeding zones * identifies the primary or target species within each zone Species are associated with certain breeding programs, seed orchards and progeny (descendant) testing installations.

To support the surveillance of key macroalgae and non-indigenous species in Nova Scotia and New Brunswick, five quantitative PCR (qPCR) assays were designed and tested at 111 sites in 2022-2023 targeting the following non-indigenous macroalgal species: Antithamnion sparsum, Bonnemaisonia hamifera, Codium fragile, Dasysiphonia japonica, Fucus serratus. All assays were developed in 2022 by the Center for Environmental Genomics Applications (CEGA, Newfoundland, Canada) except Antithamnion sparsum, for which an assay was developed in 2023 by the Aquatic Biotechnology Laboratory (ABL) at the Bedford Institute of Oceanography. All amplification was performed by the ABL in 2022-2023. The assay developed for Fucus serratus was later determined to be non-specific, and amplifies both F. serratus and Fucus distichus.Cite this data as: Krumhansl K, Brooks C, Lowen B, DiBacco C, (2025). Quantitative PCR (qPCR) of Key Macroalgal Non-Indigenous Species in Nova Scotia and New Brunswick Waters. Version 1.5. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=quantitative_qpcr_macroalgal_nonindigenous_species_novascotia_newbrunswick_2022_2023&v=1.5For additional information please see:LeBlanc F., Belliveau V., Watson E., Coomber C., Simard N., DiBacco C., Bernier R., Gagné N. 2020. Environment DNA (eDNA) detection of marine aquatic invasive species (AIS) in Eastern Canada using a targeted species-specific qPCR approach. Management of Biological Invasions 11(2):201-217Krumhansl K.A., Brooks C.M., Lowen B., O’Brien J., Wong M., DiBacco C. Loss, resilience and recovery of kelp forests in a region of rapid ocean warming. Annals of Botany 2024 Mar 8; 133(1):73-92Brooks C.M., Krumhansl K.A. 2023. First record of the Asian Antithamnion sparsum Tokida, 1932 (Ceramiales, Rhodophyta) in Nova Scotia, Canada. BioInvasions Records 12(3):745-725.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Monitoring programs are an important component of Marine Protected Area (MPA) management, providing requisite information on the state of, and changes in, protected ecosystems. Monitoring is required to gauge the efficacy of MPAs towards their conservation objectives and provides information needed to evaluate the benefits provided to biodiversity from restricted access. However, in Nova Scotia’s coastal zone, there is a lack of baseline data, including fish diversity and community structure in macrophyte beds, which makes monitoring intractable. In 2017, the Eastern Shore Islands was identified as a coastal Area of Interest (AOI) for the potential establishment of an MPA. In 2018 an overview was conducted, detailing the spatial and temporal ecological attributes of the AOI. This information revealed a unique coastal ecosystem associated with a dense archipelago and relatively natural seascape. The abundance of plant and algal biogenic habitats within the area was assumed to host a diversity of juvenile fish species. The primary objective of this project is to begin development of a long-term biodiversity monitoring program in the Eastern Shore Islands and other coastal Areas of Interest for conservation planning. We propose implementing this program with the use of direct (beach seines, scuba diving, and stable isotope sampling) and indirect (environmental DNA - eDNA) sampling. Environmental DNA (eDNA) is a useful tool to examine marine biodiversity in a non-invasive way, on a small spatial scale. eDNA can be easily collected and filtered and is becoming increasingly cost efficient to sequence and may be a useful marine protected area monitoring tool. While eDNA generally yields comparable results to traditional sampling techniques in terms of biodiversity captured, little is known on how eDNA signals fluctuate across years (or even days to weeks). We will compare species detections using eDNA metabarcoding to visual surveys (scuba and seine nets) to census eelgrass beds across the coastal zone, providing a baseline and time series of species diversity on which to base long-term monitoring. This project will generate inventories of eelgrass bed locations, and fish and invertebrate diversity within eelgrass beds. We additionally collect fish length distribution data to examine seasonal and inter-annual trends in size structure over time. The data generated from direct and indirect sampling will provide a comprehensive and ongoing catalog of species diversity and community structure in coastal eelgrass beds, as well as best-practices for sampling eDNA in the coastal environment.Cite this data as: Jeffery, N.W., Pettitt-Wade, H., Van Wyngaarden, M., and Stanley, R.R.E. Maritimes Coastal Biodiversity Monitoring Program – Beach Seining.Published: December 2023. Coastal Ecosystems Science Division, Maritimes region, Fisheries and Oceans Canada, Dartmouth NS. https://open.canada.ca/data/en/dataset/dbbcb23a-d018-4b70-b8ec-89997aded770

Effective conservation planning relies on understanding population connectivity which can be informed by genomic data. This is particularly important for sessile species like the horse mussel (Modiolus modiolus), a key habitat-forming species and conservation priority in Atlantic Canada), yet little genomic information is available to describe horse mussel connectivity patterns. We used more than 8000 restriction-site associated DNA sequencing-derived single nucleotide polymorphisms and a panel of 8 microsatellites to examine genomic connectivity among horse mussel populations in the Bay of Fundy, along the Scotian Shelf, and in the broader northwestern Atlantic extending to Newfoundland. Despite phenotypic differences between sampling locations, we found an overall lack of genetic diversity and population structure in horse mussels in the Northwest Atlantic Ocean. All sampled locations had low heterozygosity, very low FST, elevated inbreeding coefficients, and deviated from Hardy-Weinberg Equilibrium, highlighting generally low genetic diversity across all metrics. Principal components analysis, Admixture analysis, pairwise FST calculations, and analysis of outlier loci (potentially under selection) all showed no independent genomic clusters within the data, and an analysis of molecular variance showed that less than 1% of the variation within the SNP dataset was found between sampling locations. Our results suggest that connectivity is high among horse mussel populations in the Northwest Atlantic, and coupled with large effective population sizes, this has resulted in minimal genomic divergence across the region. These results can inform conservation design considerations in the Bay of Fundy and support further integration into the broader regional conservation network.Cite this data as: Van Wyngaarden, Mallory et al. (2024). Widespread genetic similarity between Northwest Atlantic populations of the horse mussel, Modiolus modiolus. Published: May 2025. Coastal Ecosystem Science Division, Maritimes Region, Fisheries and Oceans Canada, Dartmouth, NS.

Gross biological volume - total (GBVTOT) is an expression of in-the-tree stem total volume (m3) on a per-hectare basis. Calculated from the ground to the tip. Available here as a raster (GeoTIF) with a 20 m pixel resolution.Download: Here The Saskatchewan Ministry of Environment, Forest Service Branch, has developed a forest resource inventory (FRI) which meets a variety of strategic and operational planning information needs for the boreal plains. Such needs include information on the general land cover, terrain, and growing stock (height, diameter, basal area, timber volume and stem density) within the provincial forest and adjacent forest fringe. This inventory provides spatially explicit information as 10 m or 20 m raster grids and as vectors polygons for relatively homogeneous forest stands or naturally non-forested areas with a 0.5 ha minimum area and a 2.0 ha median area.  Gross biological volume per hectare - total (GBVTOT) is an expression of in-the-tree stem total volume (m3) on a per-hectare basis. Calculations are made from the ground to the tip. GBVTOT is available here as a color-mapped 16-bit unsigned integer raster grid in GeoTIFF format with a 20 m pixel resolution. An ArcGIS Pro layer file (*.lyrx) is supplied for viewing GBVTOT data in the following 50 m3/ha categories. Domain: [NULL, 0…1000]. RANGE LABEL RED GREEN BLUE 0 <= GBVTOT < 25 0 NA NA NA 25 <= GBVTOT < 75 50 63 81 181 75 <= GBVTOT < 125 100 66 101 160 125 <= GBVTOT < 175 150 68 121 138 175 <= GBVTOT < 225 200 71 140 117 225 <= GBVTOT < 275 250 74 160 96 275 <= GBVTOT < 325 300 85 178 79 325 <= GBVTOT < 375 350 123 191 74 375 <= GBVTOT < 425 400 161 203 70 425 <= GBVTOT < 475 450 198 216 66 475 <= GBVTOT < 525 500 236 229 61 525 <= GBVTOT < 575 550 255 226 53 575 <= GBVTOT < 625 600 255 209 40 625 <= GBVTOT < 675 650 255 191 28 675 <= GBVTOT < 725 700 255 174 16 725 <= GBVTOT < 775 750 255 156 3 775 <= GBVTOT < 825 800 253 139 9 825 <= GBVTOT < 875 850 251 121 20 875 <= GBVTOT < 925 900 249 103 31 925 <= GBVTOT < 975 950 246 85 43 975 <= GBVTOT <= 1000 1000 244 67 54 For more information, see the Forest Inventory Standard of the Saskatchewan Environmental Code, Forest Inventory Chapter.

Nice guys.**This third party metadata element was translated using an automated translation tool (Amazon Translate).**