This dataset contains the abundance (per m²) and the biomass (mg dry per m²) of macrofauna (≥ 500µm) in eelgrass and adjacent bare soft sediments, collected at sites in the Atlantic of Nova Scotia from 2009 to 2013.Cite this data as: Wong M.C. Data of Benthic invertebrates in seagrass and bare soft sediments in Atlantic Nova Scotia Published May 2020. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/05d5f46a-7f19-11ea-8a4e-1860247f53e3Publications: Wong, M. C., & Dowd, M. (2021). Functional trait complementarity and dominance both determine benthic secondary production in temperate seagrass beds. Ecosphere. 12(11), e03794. https://doi.org/10.1002/ecs2.3794Wong, M. C. (2018). Secondary Production of Macrobenthic Communities in Seagrass (Zostera marina, Eelgrass) Beds and Bare Soft Sediments Across Differing Environmental Conditions in Atlantic Canada. Estuaries and Coasts, 41, 536–548. https://doi.org/10.1007/s12237-017-0286-2

This layer details Important Areas (IAs) relevant to key invertebrate species (which are not corals or sponges) in the West Coast Vancouver Island (WCVI) ecoregion. This data was mapped to inform the selection of marine Ecologically and Biologically Significant Areas (EBSA). Experts have indicated that these areas are relevant based upon their high ranking in one or more of three criteria (Uniqueness, Aggregation, and Fitness Consequences). The distribution of IAs within ecoregions is used in the designation of EBSAs.Canada’s Oceans Act provides the legislative framework for an integrated ecosystem approach to management in Canadian oceans, particularly in areas considered ecologically or biologically significant. DFO has developed general guidance for the identification of ecologically or biologically significant areas. The criteria for defining such areas include uniqueness, aggregation, fitness consequences, resilience, and naturalness. This science advisory process identifies proposed EBSAs in Canadian Pacific marine waters, specifically in the Strait of Georgia (SOG), along the west coast of Vancouver Island (WCVI, southern shelf ecoregion), and in the Pacific North Coast Integrated Management Area (PNCIMA, northern shelf ecoregion).Initial assessment of IAs in PNCIMA was carried out in September 2004 to March 2005 with spatial data collection coordinated by Cathryn Clarke. Subsequent efforts in WCVI and SOG were conducted in 2009, and may have used different scientific advisors, temporal extents, data, and assessment methods. WCVI and SOG IA assessment in some cases revisits data collected for PNCIMA, but should be treated as a separate effort.Other datasets in this series detail IAs for birds, cetaceans, coral and sponges, fish, geographic features, and other vertebrates.Though data collection is considered complete, the emergence of significant new data may merit revisiting of IAs on a case by case basis.

The purpose of the survey is to document and record habitat types and associated algae and marine invertebrate species in a variety of habitat types. Transect locations are randomly selected throughout the study area, which rotates between the north and south coasts of British Columbia on a biannual basis. Transects are laid perpendicular to the shoreline. A team of two divers swim the transect with data sheets to collect habitat, algae and marine invertebrate data as detailed below in the methods section. Data is keypunched in an MS Access database that can be queried for species observations and environmental information.This dataset includes three tables pulled from the original database containing observations by species, observations by quadrat, and additional header information for each observation. All three tables can be linked by the field HKey. Three lookup tables are included as well, one for algae, one for invertebrates, and one for substrates.

This dataset documents the epifauna occurrences collected from 2021 to 2024 during the Canadian Beaufort Sea Marine Ecosystem Assessment (CBS-MEA) conducted by the Department of Fisheries and Oceans (DFO). This scientific program focuses on the integration of oceanography, food web linkages, physical-biological couplings, and spatial and interannual variabilities. The program also aims to expand the baseline coverage of species diversity, abundances, and habitat associations in previously unstudied areas of the Beaufort Sea and Western Canadian Archipelago. The study took place mainly in the Canadian Beaufort Sea and the Amundsen Gulf. Sampling is done along transects at fixed stations in the study area. Catches are collected with a 3 m benthic beam trawl for 10 minutes bottom-contact time at a target speed of 2 knots and with a modified Atlantic Western IIA otter trawl for 20 minutes bottom-contact time at a target speed of 2.9 knots. A total of 32 stations were sampled for epifauna in 2021, 22 in 2022, 23 in 2023 and 22 in 2024, between 22-655 m depth. Epibenthic invertebrates were identified to the lowest taxonomic level possible and photographed. All unknown specimens are frozen. In the lab, the identifications are validated or refined with the photos and the frozen specimens.The data are presented in Darwin Core and are separated in two files:The "Activité_épifaune_CBSMEA_epifauna_event_en" file which contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The "Occurrence_épifaune_CBSMEA_epifauna_en" file that contains the taxonomic occurrences.

The Department of Fisheries and Oceans (DFO) Science Branch has designed a multispecies dive survey protocol to provide unbiased, coast wide monitoring of benthic invertebrate stocks (as may be required under the updated Fisheries Act) and associated habitat information for a suite of benthic marine invertebrate species (Green (Strongylocentrotus droebachiensis), Purple (S. purpuratus) and Red Sea Urchin (Mesocentrotus franciscanus), Geoduck (Panopea generosa), Giant Red Sea Cucumber (Apostichopus californicus), Northern Abalone (Haliotis kamtschatkana), and Sunflower Sea Star (Pycnopodia helianthoides)). Based on information available at this time, the proposed survey design can provide estimates of coast wide stock status for Red Sea Urchin and Giant Red Sea Cucumber, and relative abundance indices for Geoduck, Green Sea Urchin, Purple Sea Urchin, Northern Abalone and Sunflower Sea Star.The new protocol was tested through a series of pilot surveys conducted on a subset of areas of the BC coast each September from 2016-2021. Design of the pilot surveys was based on previous dive survey data and experience and demonstrated the practical feasibility of the protocol, while also gathering preliminary information to guide recommendations about the statistical design of the survey.The dataset consists of a relational database containing tables representing each component of the survey methodology. The primary component of the survey is a transect location. Along each transect, systematically spaced quadrats are sampled, and on each quadrat, substrate observations are recorded, multiple species of algae are recorded, and multiple individual invertebrates are measured or counted. The tables are linked by transect number and quadrat number.

Benthic invertebrates monitoring includes both lotic (rivers/streams) and lentic (wetlands) ecosystems. Aquatic biomonitoring provides a direct measure of change in biotic populations and communities in relation to benchmark or reference conditions and can help identify the ecological effects of cumulative stressors. Used together with the water chemical and physical monitoring components, this program uses an integrated approach to assess whether ecological affects are occurring in response to OS developments. Sampling can include the collection of invertebrates, algal biomass, water chemistry, and appropriate supporting habitat information and is conducted during periods of high abundance and diversity of macroinvertebrates. Sampling focuses on near-shore gravel and sand habitats on the Athabasca River, erosional habitats on major tributaries and in wadable areas in deltaic wetlands within the Expanded Geographical Area. As of October 2012, over 80 locations have been visited.

Marine classification schemes based on abiotic surrogates often inform regional marine conservation planning in lieu of detailed biological data. However, theses chemes may poorly represent ecologically relevant biological patterns required for effective design and management strategies. We used a community-level modeling approach to characterize and delineate representative mesoscale (tens to thousands of kilometers) assemblages of demersal fish and benthic invertebrates in the North-west Atlantic. Hierarchical clustering of species occurrence data from four regional annual multispecies trawl surveys revealed three to six groupings (predominant assemblage types) in each survey region, broadly associated with geomorphic and oceanographic features. Indicator analyses identified 3–34 emblematic taxa of each assemblage type. Random forest classifications accurately predicted assemblage dis-tributions from environmental covariates (AUC > 0.95) and identified thermal limits (annual minimum and maximum bottom temperatures) as important pre-dictors of distribution in each region. Using forecasted oceanographic conditions for the year 2075 and a regional classification model, we projected assemblage dis-tributions in the southernmost bioregion (Scotian Shelf-Bay of Fundy) under ahigh emissions climate scenario (RCP 8.5). Range expansions to the north eastare projected for assemblages associated with warmer and shallower waters of the Western Scotian Shelf over the 21st century as thermal habitat on the rela-tively cooler Eastern Scotian Shelf becomes more favorable. Community-level modeling provides a biotic-informed approach for identifying broadscale ecolog-ical structure required for the design and management of ecologically coherent, representative, well-connected networks of Marine Protected Areas. When com-bined with oceanographic forecasts, this modeling approach provides a spatial tool for assessing sensitivity and resilience to climate change, which can improve conservation planning, monitoring, and adaptive management.Cite this data as: O'Brien, J.M., Stanley, R.R.E., Jeffery, N.W., Heaslip, S.W., DiBacco, C., and Wang, Z. Demersal fish and benthic invertebrate assemblages in the Northwest Atlantic.Published: December 2024. Coastal Ecosystems Science Division, Maritimes region, Fisheries and Oceans Canada, Dartmouth NS.https://open.canada.ca/data/en/dataset/14d55ea5-b17d-478c-b9ee-6a7c04439d2b

This resource documents a dataset of epifauna occurrences collected in 2021 during The Knowledge and Ecosystem-Based Approach in Baffin Bay (KEBABB) program developed by the Department of Fisheries and Oceans Canada (DFO) in collaboration with university partners. The overall objective of KEBABB is to characterize the variability and trends in physical, chemical, and biological oceanographic conditions and food webs supporting fisheries in the connected ecosystems of western Baffin Bay and Lancaster Sound. In 2021, DFO expanded the KEBABB program to Barrow Strait (KEBABS-Knowledge and Ecosystem-Based Approach in Barrow Strait), a key productive area of the Tallurutiup Imanga National Marine Conservation Area. The study took place in the Eastern Canadian Arctic (mainly in Baffin Bay, Davis Strait and Barrow Strait). Sampling is done along transects at fixed stations in the study area. Catches are collected with a 1.5 m Agassiz trawl (5 mm mesh net) for 3 minutes bottom-contact time at a target speed of 1.5 knots and with a 3 m benthic beam trawl (6.4 mm mesh net) for 15 minutes bottom-contact time at a target speed of 3 knots. A total of 16 stations were sampled for epifauna in 2021 between 85-850 m depth. Epibenthic invertebrates are identified to the lowest possible taxonomic level and photographed. All unknown specimens are frozen. In the lab, the identifications are validated or refined with the photos and the frozen specimens.The data are presented in Darwin Core and are separated in two files:The “Activité_épifaune_KEBABB_epifauna_event_en” file which contains information about missions, stations and deployments, which are presented under a hierarchical activity structure.The “Occurrence_épifaune_KEBABB_epifauna_en” file that contains the taxonomic occurrences.Further details on sampling can be found in the following report: Pućko, M., Charette, J., Tremblay P., Brulotte S., St-Denis B., Ciastek S., Hedges, K., Kuzyk, Z., Roy V., and Michel, C. 2022. An ecosystem-based approach in the eastern Arctic: KEBABB/S (Knowledge and Ecosystem-Based Approach in Baffin Bay/Barrow Strait) 2021 expedition report. Can. Manuscr. Rep. Fish. Aquat. Sci. 3250: viii + 58 p. https://publications.gc.ca/collections/collection_2022/mpo-dfo/Fs97-4-3250-eng.pdfUSE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

The North Shore of the Lower Estuary (Upper North Shore, Quebec) is a productive coastal system where many commercial species of benthic invertebrates are fished in the infralittoral (10-20 m) and circalittoral (20-50 m) zone. However, little data exist on the biodiversity of non-commercial species and the environmental characteristics of the benthic habitat in this area. Two scientific surveys were conducted in 2018 and 2019 to address this knowledge gap by developing a framework of biodiversity and environmental (water column and seafloor) data taking that will be used to determine the baseline state of the benthic ecosystem in this region.Surveys were conducted in 2018 (August 11-14) and 2019 (July 30-August 5) in the Upper North Shore region (between the towns of Forestville and Godbout). Surveys followed a fixed sampling design of eight transects perpendicular to bathymetry with stations at 10 m depth intervals in a bathymetry range of 10-50 m for a total of approximately 40 stations per survey. Specimens were collected with a beam trawl with an opening of 2.8 m. The hauls were made at a target speed of 2 knots and a target duration of 7 minutes. Start and end positions were recorded to calculate the distance traveled on each tow using the geosphere library of R. The average tow distance was approximately 425 m. The area covered at each tow was the product of the trawl opening and the distance traveled. The three files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "event_information" file includes generic event information, including date and location. The "additional_information_event_and_occurrence" file includes sample size, sampling protocol and sampling effort, among others. The "taxon_occurrence" file includes the taxonomy of the species observed, identified to the species or lowest possible taxonomic level. For abundance and biomass estimates, contact Virginie Roy (virginie.roy@dfo-mpo.gc.ca). For quality controls, all taxonomic names were checked against the World Register of Marine Species (WoRMS) to match recognized standards. The WoRMS match was placed in the "scientificNameID" field of the occurrence file. Special cases were noted in "identificationRemarks" and selected specimens were confirmed using field photos. Data quality checks were performed using the R obistools and worrms libraries. All sampling locations were spatially validated.This project was funded by DFO Coastal Environmental Baseline Program under Canada’s Oceans Protection Plan. This initiative aims to acquire environmental baseline data contributing to the characterization of important coastal areas and to support evidence-based assessments and management decisions for preserving marine ecosystems.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b