Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

Considered the "king" of sea lions, the Steller sea lion (Eumetopias jubatus) is the biggest of all sea lions and enjoys a lifespan of up to thirty years. In Canada, the Steller can be spotted along the rocky coast of British Columbia. This hefty mammal usually travels alone or in a small group, but wisely, it joins others for protection during the mating and birthing season. Little is known about its oceanic lifestyle; however, the good news for this sea-loving mammal is that since the Steller sea lion first became protected in 1970, the size of the adult population has more than doubled. Recent trends in the abundance of Steller sea lions (Eumetopias jubatus) in British Columbia were assessed based on a series of thirteen province-wide aerial surveys conducted during the breeding season (27-June to 06-July) between 1971 and 2013.

The mako shark (Isurus oxyrinchus), is a species found in Atlantic Canadian waters which is encountered in commercial and recreational fisheries. Pop-up Satellite Archival Tags (PSAT) from Wildlife Computers were applied to mako sharks from 2011 to 2013 to collect data on depth (pressure), temperature and ambient light level (for position estimation). Deployments were conducted in Canada on commercial vessels, typically in summer and fall from July to October. Two types of tag models were deployed: Mk10 (N=28), and MiniPAT (N=9) and 28 of 37 tags reported (one female shark was recaptured). The mako sharks tagged ranged in size from 80 cm to 229 cm Fork Length (curved); 13 were female, 17 were male, and 7 were unknown sex. Time at liberty ranged from 0 – 185 days and 6 tags remained on for the programmed duration. Raw data transmitted from the PSAT’s after release was processed through Wildlife Computers software (GPE3) to get summary files, assuming a maximum swimming speed of 2m/s, NOAA OI SST V2 High Resolution data set for SST reference and ETOPO1-Bedrock dataset for bathymetry reference. The maximum likelihood position estimates are available in .csv and .kmz format and depth and temperature profiles are also in .csv format. Other tag outputs as well as metadata from the deployments can be obtained upon request from: warren.joyce@dfo-mpo.gc.ca or heather.bowlby@dfo-mpo.gc.ca.

Historical finds of Fenusa pumila

The Shorezone Biobanding Polygons are an area representation of the various types of biota (flora and fauna) and their distribution, or lack thereof, found in the shoreunit.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

Situated in the Gwich’in settlement Area (GSA), the Rat River is inhabited by anadromous Dolly Varden (Salvelinus malma malma) that are harvested by both Gwich’in and Inuvialuit beneficiaries. The harvest of Dolly Varden from the Rat River occurs during the summer at feeding areas along the coast (by the Inuvialuit) and during upstream migration in the Mackenzie Delta (by both Gwich’in and Inuvialuit). Dolly Varden stocks are co-managed under an Integrated Fisheries Management Plan (IFMP) whose signatories include Fisheries and Oceans Canada (DFO), Gwich'in Renewable Resources Board, Fisheries Joint Management Committee, and Parks Canada Agency. The Rat River Working Group, the co-management body that makes recommendations for harvest levels for Dolly Varden stocks in the GSA, has supported research activities that facilitate implementation of the IFMP, including studies to monitor harvest levels and assess population status. Population studies (e.g., abundance estimates, biological and genetic sampling) and coastal harvest monitoring activities allow for a comprehensive assessment of this stock. The data are used to inform co-management partners on the status of Dolly Varden from the Rat River.

The porbeagle shark (Lamna nasus), is a species found in Atlantic Canadian waters which is encountered in commercial and recreational fisheries. Pop-up Satellite Archival Tags (PSAT) from Wildlife Computers were applied to porbeagle sharks from 2005 to 2021 to collect data on depth (pressure), temperature and ambient light level (for position estimation). Deployments were conducted in Canada and the Faroe Islands on commercial, recreational and scientific charters, typically in summer and fall but some over winter when the porbeagle commercial fishery was active in Canada. A variety of tag models were deployed: PAT 4 (n=1), Mk10 (N=41), and MiniPAT (N=15) and 51 of 57 tags reported. One individual shark was recaptured and the physical tag was returned. The porbeagle sharks tagged ranged in size from 76 cm to 249 cm Fork Length (curved); 42 were female, 15 were male. Time at liberty ranged from 4 – 356 days and 14 tags remained on for the programmed duration. Raw data transmitted from the PSAT’s after release was processed through Wildlife Computers software (GPE3) to get summary files, assuming a maximum swimming speed of 2m/s, NOAA OI SST V2 High Resolution data set for SST reference and ETOPO1-Bedrock dataset for bathymetry reference. The maximum likelihood position estimates are available in .csv and .kmz format and depth and temperature profiles are also in .csv format. Other tag outputs as well as metadata from the deployments can be obtained upon request from: warren.joyce@dfo-mpo.gc.ca or heather.bowlby@dfo-mpo.gc.ca.

Speckled Dace (Rhinichthys osculus) are listed under the Species at Risk Act (SARA) as endangered. These fresh water fish, of the family Cyprinidae, are found in Canada only in the Kettle Valley of British Columbia. Proposed critical habitat was based on minimum viable population analysis and assumed densities of fish. From October 19th to the 22nd of 2015, night time pole seining surveys were conducted to enumerate Speckled Dace in proposed critical habitat on the West Kettle River; one of three rivers containing Speckled Dace. The estimated population abundance of Speckled Dace within the survey area was 8,978 (6,143-11,814), however only 1,014 of these are estimated to be adults.

Sexual reproduction is critical to the resilience of seagrass beds impacted by habitat degradation or environmental changes, as robust seed banks allow new shoots to establish each year. Reproductive strategies of seagrass beds range on a continuum from strictly annual to perennial, driven by local environmental conditions. We examined the reproductive dynamics of Zostera marina beds at six sites on the Atlantic coast of Canada to characterize how life history strategies are shaped by the surrounding environment. Sites were categorized as wave protected and wave exposed, where protected sites were warm, shallow, with little water movement and muddy sediments, and exposed sites were either shallow or deep, with cooler water and sandy sediments. While mixed life history strategies were evident at all sites, protected eelgrass beds exhibited both the highest and lowest sexual reproductive effort relative to exposed beds. These beds regularly experienced thermal stress, with higher temperature range and extended warm water events relative to exposed beds. The development of reproductive shoots were similar across sites with comparable Growing Degree-days at the beginning and end of anthesis, but the First Flowering Date was earlier at the protected warmer sites relative to exposed sites. With different reproductive shoot density among sites, seed production, seed retention, and seedling recruitment also varied strongly. Only one site, located in a warm, shallow and protected lagoon, contained a mixed life history population with a high reproductive effort (33.7%), strong seed bank, and high seedling establishment. However, a primarily perennial population with the lowest reproductive effort (0.5%) was identified at the warmest site, suggesting that conditions here could not support high sexual reproduction. Robustness of seed banks was strongly linked to reproductive shoot density, although the role of seed retention, germination and seedling survival require further investigation. Our study provides insights into one key aspect of seagrass resilience, and suggests that resilience assessments should include reproductive shoot density to inform their management and conservation.Cite this data: Vercaemer B. and Wong M. Reproductive ecology of Zostera marina L. (eelgrass) across varying environmental conditions. Published: May 2022. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/56cfea6f-aeca-47ed-94ab-c519d9e63c91