Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes.Cite this data as: Jeffery, N., Rubidge, E., Abbott, C., Westfall, K., Stanley, R. (2024): Data of: eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment.Published: August 2024. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/43a91ba7-8025-4330-88db-db14022d729d

Monitoring programs are an important component of Marine Protected Area (MPA) management, providing requisite information on the state of, and changes in, protected ecosystems. Monitoring is required to gauge the efficacy of MPAs towards their conservation objectives and provides information needed to evaluate the benefits provided to biodiversity from restricted access. However, in Nova Scotia’s coastal zone, there is a lack of baseline data, including fish diversity and community structure in macrophyte beds, which makes monitoring intractable. In 2017, the Eastern Shore Islands was identified as a coastal Area of Interest (AOI) for the potential establishment of an MPA. In 2018 an overview was conducted, detailing the spatial and temporal ecological attributes of the AOI. This information revealed a unique coastal ecosystem associated with a dense archipelago and relatively natural seascape. The abundance of plant and algal biogenic habitats within the area was assumed to host a diversity of juvenile fish species. The primary objective of this project is to begin development of a long-term biodiversity monitoring program in the Eastern Shore Islands and other coastal Areas of Interest for conservation planning. We propose implementing this program with the use of direct (beach seines, scuba diving, and stable isotope sampling) and indirect (environmental DNA - eDNA) sampling. Environmental DNA (eDNA) is a useful tool to examine marine biodiversity in a non-invasive way, on a small spatial scale. eDNA can be easily collected and filtered and is becoming increasingly cost efficient to sequence and may be a useful marine protected area monitoring tool. While eDNA generally yields comparable results to traditional sampling techniques in terms of biodiversity captured, little is known on how eDNA signals fluctuate across years (or even days to weeks). We will compare species detections using eDNA metabarcoding to visual surveys (scuba and seine nets) to census eelgrass beds across the coastal zone, providing a baseline and time series of species diversity on which to base long-term monitoring. This project will generate inventories of eelgrass bed locations, and fish and invertebrate diversity within eelgrass beds. We additionally collect fish length distribution data to examine seasonal and inter-annual trends in size structure over time. The data generated from direct and indirect sampling will provide a comprehensive and ongoing catalog of species diversity and community structure in coastal eelgrass beds, as well as best-practices for sampling eDNA in the coastal environment.Cite this data as: Jeffery, N.W., Pettitt-Wade, H., Van Wyngaarden, M., and Stanley, R.R.E. Maritimes Coastal Biodiversity Monitoring Program – Beach Seining.Published: December 2023. Coastal Ecosystems Science Division, Maritimes region, Fisheries and Oceans Canada, Dartmouth NS. https://open.canada.ca/data/en/dataset/dbbcb23a-d018-4b70-b8ec-89997aded770

Fisheries and Oceans Canada (DFO) conducts research and undertakes monitoring surveys of the marine and freshwater environment in support of sustainable fisheries, healthy aquatic ecosystems and living resources, and safe and effective marine services. In an effort to effectively inform and ensure Canadians feel engaged in the delivery of its science mandate, DFO produces Fieldnotes, an annual compendium of planned science field operations in the North Pacific and Arctic oceans, as well as in the coastal and interior waters of British Columbia and Yukon.

We evaluated an autonomous environmental DNA sampler produced by Dartmouth Ocean Technologies Inc (Dartmouth, Canada) compared to time-at-sample filtration in the laboratory to determine the performance of moored samplers for monitoring in the marine world. We deployed three autonomous samplers from DOT in the Bedford Basin (Canada) over a nine-week period in summer/fall 2023. The samplers filtered seawater in situ at programmed interviews over this time period, and we collected contemporaneous samples with a standard vacuum pump during each sampling period. Both eDNA sample types captured similar fish diversity, including typical diversity for the Northwest Atlantic. The invertebrate community detected using the COI marker was different between each sample type, likely due to differences in filter pore size. We found biofouling on the moored samplers was minimal over the study period, even in a high-traffic area such as the Bedford Basin, likely due to the relatively short experimental period, and copper screening covering in the inlet and outlet valves of the instruments. Overall, our results show promise to deploy autonomous eDNA samplers in marine conservation areas to contribute to monitoring in the temperate ocean, but further testing over longer periods of time is needed to determine if DNA remains well-preserved in the autonomous samplers at ambient ocean temperatures.Cite this data as: Jeffery, N.W., Van Wyngaarden, M., and Stanley, R.R.E. Evaluating an Autonomous eDNA Sampler for Marine Environmental Monitoring: Short- and Long-Term Applications. Published: December 2024. Coastal Ecosystems Science Division, Maritimes Region, Fisheries and Oceans Canada, Dartmouth NS.

The Riparian Quality Index (IQBR) is used to assess the ecological condition of the shoreline environment. It is built from nine components that have been weighted according to their potential to perform ecological functions in terms of the protection of aquatic ecosystems.Data on shoreline quality come from benthos monitoring stations sampled between 2003 and 2023.**This third party metadata element was translated using an automated translation tool (Amazon Translate).**

Oceanographic data from stationary moorings deployed as part of the Centre for Marine Applied Research's (CMAR) Coastal Monitoring Program.

Oceanographic data from stationary moorings deployed as part of the Centre for Marine Applied Research's (CMAR) Coastal Monitoring Program.

Oceanographic data from stationary moorings deployed as part of the Centre for Marine Applied Research's (CMAR) Coastal Monitoring Program.

Oceanographic data from stationary moorings deployed as part of the Centre for Marine Applied Research's (CMAR) Coastal Monitoring Program.

Oceanographic data from stationary moorings deployed as part of the Centre for Marine Applied Research's (CMAR) Coastal Monitoring Program.