Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes.Cite this data as: Jeffery, N., Rubidge, E., Abbott, C., Westfall, K., Stanley, R. (2024): Data of: eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment.Published: August 2024. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/43a91ba7-8025-4330-88db-db14022d729d

Polygon layer containing an archaeological reference grid established by Charles Borden. Similar to the BCGS grid or TRIM grid system. All archaeological sites in Canada are numbered based on this grid

This data set contains a small sub-set of the WHSE_FOREST_TENURE.FTEN_RECREATION_POLY_SVW layer, in point form, with minimal attribution for the purpose of displaying a select set of high value recreation sites. For access to complete listing of Forest Recreation sites please use [Recreation Polygons WHSE_FOREST_TENURE.FTEN_RECREATION_POLY_SVW](http://catalogue.data.gov.bc.ca/dataset/263338a7-93ee-49c1-83e8-13f0bde70833)

This is a spatial layer showing Ministry of Forests Map Notation Lines. These are the linear spatial representation for a notation on the Forest Atlas which records the area of interest of other government agencies and individuals

This is a spatial layer showing Ministry of Forests Map Notation Polygons. These are polygonal spatial representation for a notation on the Forest Atlas which records the area of interest of other government agencies and individuals

Public parking spaces in downtown Sherbrooke.attributes:Location - Location of the parking spaceName - Name of the parkingNumber - Number of the parking meterType - Type of parkingLevel - Level of the parking spaceLevel - Level of the parking space**This third party metadata element was translated using an automated translation tool (Amazon Translate).**

Buildings located on the territory of the City of Sherbrooke and belonging to one of the following categories: business, hospital, school or municipal building. These categories are respectively associated with subtype codes 2, 3, 4, and 5 attributes:ID - Unique IdentifierSubtype - Building Subtype Code (2 - Business, 3 - Hospital, 4 - Hospital, 4 - School, 4 - School, 5 - Municipal Building)**This third party metadata element was translated using an automated translation tool (Amazon Translate).**

The purpose of this dataset is to give an accurate representation of the boundaries of lands closed to hunting in Manitoba.The purpose of this dataset is to give an accurate representation of the boundaries of lands closed to hunting in Manitoba. These are defined as lands where hunting and trapping are either prohibited, or special restrictions are in place for certain species and areas are defined within the General Hunting Regulation (351/87), Moose Conservation Closure Regulation (122/2011), Hunting Seasons and Bag Limits Regulation (165/91), and Trapping of Wild Animals Regulation (245/90) of The Wildlife Act (C.C.S.M c. W130). Hunting and trapping prohibitions or restrictions are implemented in certain areas to ensure the conservation of species or enhance public safety. Fields included (Alias (Field Name): Field description) FID (OBJECTID): Sequential unique whole numbers that are automatically generated Id (Id): The number assigned to each restricted area (not currently being used) Name (Name): Name given to the restricted area Restrictions (Restrictions): Description of the restriction applied to the restricted area Director of Surveys Plan (D_of_S): Director of Surveys Plan number which pertains to the boundary of the restricted area Regulation (Regulation): The regulation title defined in The Wildlife Act Shape_Area (Shape_Area): Area of the feature in internal units squared Shape_Length (Shape_Length): Length of the feature in internal units

The purpose of this feature class is to show areas that are National Parks (NPs), National Park Reserves (NPRs) and National Marine Conservation Area (NMCAs). Parks Canada manages more than 43 NPs. (See: https://parks.canada.ca/voyage-travel/recherche-tous-parks-all) for a list of PCA administered parks.By using this data, it is important to understand and accept that the data is not to be used for defining boundaries. Administrative decisions should be based on legal documents and legal survey plans.Canada Lands Surveys: https://clss.nrcan-rncan.gc.ca/clss/plan/search-recherche

Land Features entities are: Island, Shoreline, Wooded Area, Saturated soil, Landform Feature (esker, sand\...), and Cut Line. CanVec is a digital cartographic reference product of Natural Resources Canada (NRCan). It originates from the best available data sources covering Canadian territory, offers quality topographical information in vector format, and complies with international geomatics standards. CanVec is a multi-source product coming mainly from the National Topographic Data Base (NTDB), the Mapping the North process conducted by the Canada Center for Mapping and Earth Observation (CCMEO), the Atlas of Canada data, the GeoBase initiative, and the data update using satellite imagery coverage (e.g. Landsat 7, Spot, Radarsat, etc.).Distributed from [GeoYukon](https://yukon.ca/geoyukon) by the [Government of Yukon](https://yukon.ca/maps) . Discover more digital map data and interactive maps from Yukon's digital map data collection.For more information: [geomatics.help@yukon.ca](mailto:geomatics.help@yukon.ca)