Marine protected areas (MPAs) are one among a number of spatial management tools, and are defined as areas that are established for the long-term, and managed through legal or other effective means, to achieve the long-term conservation of nature with associated ecosystem services and cultural values.Currently, Fisheries and Oceans Canada has a number of MPAs designated under the Oceans Act and Areas of Interest for new MPAs at various stages of progress towards designation. These areas are ecologically significant, with species and/or features that require special management consideration.  An Oceans Act MPA can be established for any of the six conservation purposes outlined in the Act:• The conservation and protection of commercial and non-commercial fishery resources, including marine mammals, and their habitats;  • The conservation and protection of endangered or threatened marine species, and their habitats;  • The conservation and protection of unique habitats;  • The conservation and protection of marine areas of high biodiversity or biological productivity; • The conservation and protection of any other marine resource or habitat as is necessary to fulfill the mandate of the Minister; and• The conservation and protection of marine areas for the purposes of maintaining ecological integrity

Appropriately responding to a marine pollution event, especially hydrocarbon spills, often requires detailed knowledge of local habitat and environmental features. Access to high resolution habitat profiles can support effective spill response plans, informing discussions on protection priorities or expediated remediation. However, marine habitat composition data for a given area is often lacking due to the high cost and effort of conducting such surveys across the vast shorelines of Canada. The purpose of this study was to develop methodologies for conducting rapid and affordable habitat compositions in the marine environment via drone aerial photography; an emerging technology for conducting high resolution surveys. We used the Musquash Marine Protected Area (MPA; Musquash, NB, Canada) as a model system as it contains a diverse range of habitat types, is a region of conservation concern in Atlantic Canada, and is in close proximity to oil and gas handling facilities and vessel traffic. The MPA consists of a tidal river that outflows into the Bay of Fundy. Using Geographic Information System (GIS) software, we subdivided the MPA into several transects (N =61) that were used to generate flight plans for a Remotely Piloted Aircraft System (RPAS; DJI Mavic 3 Enterprise, DJI ). The RPAS was flown (6 m s-1) at an altitude of 100m (Above ground level) taking images with side (70%) and front (80%) overlap. Resulting images were then compiled as an orthomosaic map using Pix4Dmatic software. These data will be used to inform marine spill response planning in the region, to support marine planning and conservation, and Marine Protected Area (MPA) monitoring efforts as well as develop further methodological approaches for conducting RPAS-based habitat surveys in other coastal systems within Atlantic Canada. Cite this data as: Lawrence MJ, Coates PJ, Matheson K, Hamer A. Characterisation of intertidal habitat types in the Musquash Marine Protected Area using aerial drone photography. Published November 2025. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, St. Andrews, N.B.

The Great Lakes Fish Biodiversity Science Database is a compilation of fish community and habitat data from DFO Science surveys, primarily related to freshwater fishes of conservation concern in the Great Lakes basin. Data include: sampling site location, date, fish species and counts, and associated habitat information. Project-specific details including purpose/objectives and study methodology are often reported in the DFO Canadian data report of fisheries and aquatic sciences series.

Identification of a potential spawning site for lumpfish during an ichtyofauna inventory conducted in the eelgrass in Sept-Îles bay by Calderón (1996).Purpose:The 1996 document by par Isabel Calderón has been achieved by the "Corporation de protection de l'environnement de Sept-Îles" with the support of Department of Fisheries and Oceans (DFO) as part of the "Biodiversity" program, St. Lawrence Vision 2000 (Canada).Source:Calderón, I. 1996. Caractérisation des habitats du poisson de la baie de Sept-Îles - Phase II. Corporation de protection de l'environnement de Sept-Îles. 37 pages.

Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes.Cite this data as: Jeffery, N., Rubidge, E., Abbott, C., Westfall, K., Stanley, R. (2024): Data of: eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment.Published: August 2024. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/43a91ba7-8025-4330-88db-db14022d729d

Funded under DFO's Marine Conservation Targets Program in partnership with the Huntsman Marine Science Centre (HMSC), this diver-based imagery and sample collection benthic survey documents the occurrence of sponges at 42 dive sites in the Eastern Shore Islands (ESI) Area of Interest (AOI, ~2089 km2) off the Atlantic coast of Nova Scotia, Canada from dive surveys conducted in summer 2021 and 2022. Water quality, species occurrences and counts, habitat, slope, and substrate characteristics were catalogued through diver log sheets, camera imagery, specimen vouchers, and high-resolution bathymetric data. A total of 54 dives to depths from 11 to 33 m (below sea level), collecting up to 147 still images, one-hour of video, and 17 specimen samples per site, resulted in 220 observations for 27 different sponge taxa. This included three new records for Canada (Hymedesmia stellifera, Plocamionida arndti, Hymedesmia jecusculum) and a range extension for a species new to science (Crellomima mehqisinpekonuta) which was recently described from the Bay of Fundy. There were also four species which may seem to be new to science (Halichondria sp., Hymedesmia sp., Protosuberires sp., and Sphaerotylus sp.). Sponges were found to occupy a diversity of micro-habitats, often several different ones in proximity. A total of eight distinct habitat classes were defined, based on varying abundances and diversity of sponges and associated benthic species. These are likely widely distributed among the many complex submerged seabed features within this AOI. Collected specimens were preserved and are stored at the Atlantic Reference Centre (ARC) in St. Andrew's, New Brunswick.Cite this data as: Goodwin, C., Cooper, J.A., Lawton, P., Teed, L.L. 2025. Sponge occurrence and associated species and habitat descriptions derived from the 2021 and 2022 SCUBA diving surveys in the Eastern Shore Islands Area of Interest, Nova Scotia. Version 1.4. Fisheries and Oceans Canada. Occurrence dataset. https://ipt.iobis.org/obiscanada/resource?r=eastern_shore_islands_sponge_survey_2021_2022&v=1.4

Monitoring programs are an important component of Marine Protected Area (MPA) management, providing requisite information on the state of, and changes in, protected ecosystems. Monitoring is required to gauge the efficacy of MPAs towards their conservation objectives and provides information needed to evaluate the benefits provided to biodiversity from restricted access. However, in Nova Scotia’s coastal zone, there is a lack of baseline data, including fish diversity and community structure in macrophyte beds, which makes monitoring intractable. In 2017, the Eastern Shore Islands was identified as a coastal Area of Interest (AOI) for the potential establishment of an MPA. In 2018 an overview was conducted, detailing the spatial and temporal ecological attributes of the AOI. This information revealed a unique coastal ecosystem associated with a dense archipelago and relatively natural seascape. The abundance of plant and algal biogenic habitats within the area was assumed to host a diversity of juvenile fish species. The primary objective of this project is to begin development of a long-term biodiversity monitoring program in the Eastern Shore Islands and other coastal Areas of Interest for conservation planning. We propose implementing this program with the use of direct (beach seines, scuba diving, and stable isotope sampling) and indirect (environmental DNA - eDNA) sampling. Environmental DNA (eDNA) is a useful tool to examine marine biodiversity in a non-invasive way, on a small spatial scale. eDNA can be easily collected and filtered and is becoming increasingly cost efficient to sequence and may be a useful marine protected area monitoring tool. While eDNA generally yields comparable results to traditional sampling techniques in terms of biodiversity captured, little is known on how eDNA signals fluctuate across years (or even days to weeks). We will compare species detections using eDNA metabarcoding to visual surveys (scuba and seine nets) to census eelgrass beds across the coastal zone, providing a baseline and time series of species diversity on which to base long-term monitoring. This project will generate inventories of eelgrass bed locations, and fish and invertebrate diversity within eelgrass beds. We additionally collect fish length distribution data to examine seasonal and inter-annual trends in size structure over time. The data generated from direct and indirect sampling will provide a comprehensive and ongoing catalog of species diversity and community structure in coastal eelgrass beds, as well as best-practices for sampling eDNA in the coastal environment.Cite this data as: Jeffery, N.W., Pettitt-Wade, H., Van Wyngaarden, M., and Stanley, R.R.E. Maritimes Coastal Biodiversity Monitoring Program – Beach Seining.Published: December 2023. Coastal Ecosystems Science Division, Maritimes region, Fisheries and Oceans Canada, Dartmouth NS. https://open.canada.ca/data/en/dataset/dbbcb23a-d018-4b70-b8ec-89997aded770

Marine classification schemes based on abiotic surrogates often inform regional marine conservation planning in lieu of detailed biological data. However, theses chemes may poorly represent ecologically relevant biological patterns required for effective design and management strategies. We used a community-level modeling approach to characterize and delineate representative mesoscale (tens to thousands of kilometers) assemblages of demersal fish and benthic invertebrates in the North-west Atlantic. Hierarchical clustering of species occurrence data from four regional annual multispecies trawl surveys revealed three to six groupings (predominant assemblage types) in each survey region, broadly associated with geomorphic and oceanographic features. Indicator analyses identified 3–34 emblematic taxa of each assemblage type. Random forest classifications accurately predicted assemblage dis-tributions from environmental covariates (AUC > 0.95) and identified thermal limits (annual minimum and maximum bottom temperatures) as important pre-dictors of distribution in each region. Using forecasted oceanographic conditions for the year 2075 and a regional classification model, we projected assemblage dis-tributions in the southernmost bioregion (Scotian Shelf-Bay of Fundy) under ahigh emissions climate scenario (RCP 8.5). Range expansions to the north eastare projected for assemblages associated with warmer and shallower waters of the Western Scotian Shelf over the 21st century as thermal habitat on the rela-tively cooler Eastern Scotian Shelf becomes more favorable. Community-level modeling provides a biotic-informed approach for identifying broadscale ecolog-ical structure required for the design and management of ecologically coherent, representative, well-connected networks of Marine Protected Areas. When com-bined with oceanographic forecasts, this modeling approach provides a spatial tool for assessing sensitivity and resilience to climate change, which can improve conservation planning, monitoring, and adaptive management.Cite this data as: O'Brien, J.M., Stanley, R.R.E., Jeffery, N.W., Heaslip, S.W., DiBacco, C., and Wang, Z. Demersal fish and benthic invertebrate assemblages in the Northwest Atlantic.Published: December 2024. Coastal Ecosystems Science Division, Maritimes region, Fisheries and Oceans Canada, Dartmouth NS.https://open.canada.ca/data/en/dataset/14d55ea5-b17d-478c-b9ee-6a7c04439d2b

The Planning for an Environmental Response (PIER) initiative falls under the umbrella of the Government of Canada’s Oceans Protection Plan (OPP), whose goal is preserving marine ecosystems vulnerable to increased transportation and the development of the marine industry. The PIERs’ main mandate is to acquire and update biological sensitivity data under its jurisdiction for preparation and response purposes in the event of an oil spill.This dataset contains all observations of marine organisms noted during the analysis of 2959 underwater images sampled over a large extent of the coastal zone (≤10 m) of the Estuary and the Gulf of St. Lawrence (Quebec region). The dataset includes 21 490 occurrences of 150 taxa and informal categories including macroalgae, invertebrates and fish. Underwater images were collected between 2017 and 2021 according to a directed sampling protocol whose primary goal was to map large seaweed and eelgrass beds. Images were normally recorded as videos using a GoPro Hero camera installed on a pole and placed near the seabed from a small boat. The collected data served primarily as ground-truth data to validate coasting zone mapping based on aerial photographs within the framework of the PIER's initiative.The two files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "event_information" file includes generic event information, including date and location. The "taxon_occurrence" file includes the original identifiers of the observed organisms (verbatimIdentification field), identification remarks and their taxonomy.Taxonomic names were verified on the World Register of Marine Species (WoRMS) to match recognized standards. The WoRMS match has been put in the scientificNameID field in the occurrence file. Data quality control was performed using the R packages obistools and worrms. All sampling locations were plotted on a map to perform a visual check confirming that the latitude and longitude coordinates were within the described sampling area.A visual dictionary was developed as an identification aid and accompanies this dataset (unilingual french only, the English version will be published soon). More data, including a visibility index, estimated macroalgae and eelgrass cover, substrate type and dominant macroalgae and animals were recorded but not included in this dataset. These data may be made available upon request.CreditsProvencher-Nolet, L., Paquette, L., Pitre, L.D., Grégoire, B. and Desjardins, C. 2024. Cartographie des macrophytes estuariens et marins du Québec. Rapp. Tech. Can. Sci. halieut. Aquat. 3617 : v + 99 p.Grégoire, B., Pitre, L.D., Provencher-Nolet, L., Paquette, L. and Desjardins, C. 2024. Distribution d’organismes marins de la zone côtière peu profonde du Québec recensés par imagerie sous-marine de 2017 à 2021. Rapp. tech. can. sci. halieut. aquat. 3616 : v + 78 p.

Effective fisheries and habitat management processes require knowledge of the distribution of areas of high ecological or biological significance. On the Scotian Shelf and Slope, a number of benthic ecologically or biologically significant areas consisting of habitat-forming species such as sponges and deep-water corals have been identified. However, knowledge of their spatial distribution is largely based on targeted surveys that are limited in their spatial extent. We used a species distribution modelling approach called random forest (RF) to predict the probability of occurrence and biomass of sponges, sea pens, and large and small gorgonian corals across the entire spatial extent of Fisheries and Oceans Canada’s (DFO) Maritimes Region. We also modelled the rare sponge Vazella pourtalesi, which forms the largest known aggregation of its kind on the Scotian Shelf. We utilized a number of data sources including DFO multispecies trawl catch data and in situ benthic imagery observations. Most models had excellent predictive capacity with cross-validated Area Under the Receiver Operating Characteristic Curve (AUC) values ranging from 0.760 to 0.977. Areas of suitable habitat were identified for each taxon and were contrasted against their known distribution and when applicable, the location of closure areas designated for their protection. Generalized additive models (GAMs) were developed to predict the biomass distribution of each taxonomic group and serve as a comparison to the RF models. The RF and GAM models provided comparable results, although GAMs provided superior predictions of biomass along the continental slope for some taxonomic groups. In the absence of data observations, the results of this study could be used to identify the potential distribution of sensitive benthic taxa for use in fisheries and habitat management applications. These results could also be used to refine significant concentrations of these taxa as identified through the kernel density analyses.Cite this data as: Beazley, Lindsay; Kenchington, Ellen; Murillo-Perez, Javier; Lirette, Camille; Guijarro-Sabaniel, Javier; McMillan, Andrew; Knudby, Anders (2019). Species Distribution Modelling of Corals and Sponges in the Maritimes Region for Use in the Identification of Significant Benthic Areas. Published July 2023. Ocean Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/356e92f3-5bf3-4810-98b1-3e10cd7742aa