Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.

Considered the "king" of sea lions, the Steller sea lion (Eumetopias jubatus) is the biggest of all sea lions and enjoys a lifespan of up to thirty years. In Canada, the Steller can be spotted along the rocky coast of British Columbia. This hefty mammal usually travels alone or in a small group, but wisely, it joins others for protection during the mating and birthing season. Little is known about its oceanic lifestyle; however, the good news for this sea-loving mammal is that since the Steller sea lion first became protected in 1970, the size of the adult population has more than doubled. Recent trends in the abundance of Steller sea lions (Eumetopias jubatus) in British Columbia were assessed based on a series of thirteen province-wide aerial surveys conducted during the breeding season (27-June to 06-July) between 1971 and 2013.

Rouyn-Noranda cycling network**This third party metadata element was translated using an automated translation tool (Amazon Translate).**

Yukon Oil and Gas Rights Disposition Process. Call for Bids (CFB) - an invitation to submit bids for posted locations. The Call for Bids dataset contains basic information on disposition process administered by Yukon Government. For more information on disposition process visit [https://yukon.ca/en/doing-business/licensing/apply-oil-and-gas-rights#disposition-overview](https://yukon.ca/en/doing-business/licensing/apply-oil-and-gas-rights)Distributed from [GeoYukon](https://yukon.ca/geoyukon) by the [Government of Yukon](https://yukon.ca/maps) . Discover more digital map data and interactive maps from Yukon's digital map data collection.For more information: [geomatics.help@yukon.ca](mailto:geomatics.help@yukon.ca)

Historical finds of Operophtera brumata

Represent the competitive Yukon Oil and Gas Rights disposition process governed by the Yukon's Oil and Gas Act and administered by Government of Yukon. The disposition process has been designed so that the oil and gas industry is able to request locations for which they intend to submit bids. Request for Posting (RFP) - an expression of interest from industry to acquire oil and gas rights in a specific location. For more information visit https://yukon.ca/en/doing-business/licensing/apply-oil-and-gas-rights#disposition-overviewDistributed from [GeoYukon](https://yukon.ca/geoyukon) by the [Government of Yukon](https://yukon.ca/maps) . Discover more digital map data and interactive maps from Yukon's digital map data collection.For more information: [geomatics.help@yukon.ca](mailto:geomatics.help@yukon.ca)

AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas.Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador.Reference:TunicatesVase tunicate (Ciona intestinalis) 2012 The Vase tunicate, is a high impact solitary invader and was first detected by DFO in 2012 on the Burin Peninsula at Ship Cove and Little Bay, Placentia Bay. Various mitigation measures (McKenzie et al. 2016b) contained this invasive tunicate to a small area for six years within Placentia Bay. First detected in Fortune Bay as an established population in 2019, increasing reports of the Vase tunicate have been made along the south coast of Newfoundland. The data provided here indicates the detections of this AIS in coastal NL.From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

A federal electoral district is an area represented by a member of the House of Commons. The Federal Electoral Districts (FED) dataset is a digital representation of the 338 electoral districts proclaimed by the Representation Order of 2013. This dataset is an update of the 2013 Federal Electoral Districts (FED) 2013.

The dataset contains the digital boundaries for significant discovery licences, production licences, drilled wells, major pipelines and the jurisdictional boundary of the area administered by the Canada-Nova Scotia Offshore Energy Regulator (CNSOER), as well as the inactive Georges Bank permits formerly administered by the Canada Oil and Gas Lands Administration (COGLA). All production facilities have been removed and active projects were fully abandoned and decommissioned by November 2020. As of January 2022 there are no active exploration licences.A significant discovery licence (SDL) may be issued for lands within a declared significant discovery area under the Accord Acts. The term of a significant discovery licence is indefinite and was designed to maintain an explorer's rights during the period between first discovery and eventual production. Significant discovery licence interest representatives in 2021 include BP Canada Energy Company, ExxonMobil Canada Ltd., ExxonMobil Canada Properties, Ovintiv Canada ULC, and Shell Canada Limited. Most of the SDLs predate the CNSOEB; they were issued at various times from 1980 to 1986 by COGLA before the Offshore Board existed. On January 5, 1990, they were all rolled into CNSOEB’s governance. The only exception is SDL 2702 which was issued June 6, 2007.A production licence (PL) gives interest holders the right to produce petroleum in an area which is subject to a declared commercial discovery. In order to be declared a commercial discovery, an operator must demonstrate that the discovery contains reserves that will justify the investment of capital and effort to bring the discovery to production. A production licence has a term of 25 years but may be extended if commercial production is continuing or is likely to recommence.Production licence interest representatives in 2021 include ExxonMobil Canada Ltd. and Ovintiv Canada ULC. The PLs were effective on the following dates:• 2901, 2902: 1991-04-01• 2903, 2904, 2905, 2906: 1999-07-06• 2907: 2003-10-31• 2908: 2004-11-25• 2909, 2910, 2911: 2010-12-31 Inactive Georges Bank permit interest representatives include BP Canada Energy Company and Chevron Canada Ltd.Each production licence, significant discovery licence and inactive Georges Bank permit polygon is a mapped representation of the permit interest abstract. The offshore area is divided into grid areas and permit interest abstracts are defined by this land division. The land division system consists of grid areas, sections, and units – all referenced to the North American Datum of 1927 (NAD27). For more information on the Canada lands defined areas (Land Division), See: Canada oil and gas regulations land division (https://laws-lois.justice.gc.ca/eng/regulations/C.R.C.%2C_c._1518/page-1.html#h-525569 or https://www.cnsopb.ns.ca/resource-library/land-division-guideline).The area of a PL or SDL can be found in the permit interest abstract for that licence, available for download on the CNSOER's website (https://www.cnsopb.ns.ca/sites/default/files/resource/interest_summary_table.pdf) . The directory of wells point dataset contains the surface locations for all wells drilled in Offshore Nova Scotia. The file includes well names, locations, spud dates, termination dates, rig table elevations, water depths, well total depth, well type (exploratory, delineation, production, or injector), well result, and current status. Original data for wells drilled prior to 1990 was collected and maintained by the Geological Survey of Canada and COGLA. Data are also available on the Basin database online (https://basin.gdr.nrcan.gc.ca/index_e.php). CNSOER’s directory of wells was last updated January, 2020.Jurisdictional boundary point and lines datasets define the outer limit of the jurisdiction of the CNSOER, as described in the Canada-Nova Scotia Offshore Petroleum Resources Accord Implementation Act - Schedule 1 (Canada-Nova Scotia Offshore Petroleum Resources Accord Implementation Act (https://laws-lois.justice.gc.ca/eng/acts/C-7.8/page-1.html)), excluding the 200-nautical mile exclusive economic zone (EEZ) limit as described under Part VI of the United Nations Convention on the Law of the Sea (https://www.un.org/Depts/los/convention_agreements/texts/unclos/unclos_e.pdf?msclkid=5309c2b9cfc911ecb5bb107f67772ec1). The offshore gas pipeline polyline dataset contains locations of major pipelines in the CNSOER's jurisdictional area, associated with petroleum production in offshore Nova Scotia. This file is considered to be approximate and is not an official record. Some pipeline coordinates are sourced from CAD documents (DXF), supplied by operators. Some were created using ArcMap, digitized from paper records. As of October 2021, all pipelines within this file have been fully flushed with sea water and abandoned.All shapefiles were transformed into the NAD83 datum using the NTv2 transformation.

Canada is divided into 338 electoral districts. A representative or member of Parliament is elected for each electoral district. Following the release of population counts from each decennial census, the Chief Electoral Officer determines the number of seats in the House of Commons and publishes the information in the Canada Gazette. Electoral boundaries commissions then determine the adjustments to the constituency boundaries. The federal electoral boundaries commissions are independent bodies that make all decisions regarding the proposed and final federal electoral boundaries. Elections Canada provides support services to the boundaries commission in each province. Based on reports from these commissions, the Chief Electoral Officer prepares a representation order that describes the boundaries and specifies the name and the population of each FED. The representation order is in force on the first dissolution of Parliament that occurs at least seven months after its proclamation. The 2013 Representation Order (proclaimed on October 5, 2013) was based on 2011 Census population counts, and increased the number of FEDs to 338, up from 308 from the previous 2003 Representation Order. Ontario received fifteen additional seats, Alberta and British Columbia each gained six seats while Quebec added three seats. On June 19, 2014, the Riding Name Change Act, 2014 (Bill C-37) received Royal Assent changing the names of 31 FEDs. The names of FEDs may change at any time through an Act of Parliament.