Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

These data sets provide information pertaining to shrimp and bycatch estimates associated with otter-trawling and trapping (November, 2000) and beam-trawling (February, 2001) in Simoom Sound. Data sets were compiled and formatted by Meagan Mak.Abstract from report:As part of a project investigating possible modification of marine ecosystems by shrimp trawling and trapping, we obtained information on catches offish, shrimp, prawns , and bycatch organisms as well as weight, sex ratios , egg location and colIateral damage to several species of pandalids and eualids. Focusing on the humpback shrimp (Pandalus hypsinotus), we assessed damage to the rostrum, carapace, abdomen, and tail fan on specimens caught by beam trawling, otter trawling, and trapping. Data are given from a preliminary study conducted in Northumberland Channel in June 2000 and more comprehensive sampling from Simoom Sound in November 2000 and February 2001.

Historical finds of Operophtera brumata

Historical finds of Pristiphora erichsonii

Historical finds of Coleophora laricella

A literature review, focusing on oil sand products (e.g., diluted bitumen), diluents, spill-treating agents, and crude oil toxicology and ecological studies, relevant to the northeast Pacific was compiled as part of the Government of Canada’s World Class Tanker Safety program. Of the 763 references identified, 14 involved diluted bitumen and other heavy crude oils, indicating the need for further research of these products in the marine environment. Diluent research suggests relatively fast evaporation and dispersion times for this component, however high toxicities may pose a threat to marine biota. Historical studies indicate older dispersant formulations had potential ecological implications, therefore newer formulations, which have not been studied in detail, require full assessment. Consistent utilization of toxicology standards remains elusive, hindering species sensitivity analyses. Exxon Valdez literature demonstrates highly variable impacts from a single oil type and the need for baseline data, recovery status, and suitable ecological end-point determination.

2 Billion Trees ProgramForests and trees sustain life on Earth. Beyond the jobs that our sustainably managed forests provide, people living in Canada rely on forests for a wealth of benefits. Healthy forest ecosystems sustain thousands of living organisms, supply us with food, provide shelter and shade on a sunny day, clean the air we breathe and the water we drink, and hold spiritual significance for many, particularly within Indigenous cultures.The following 10 tree planting projects (out of 72 projects funded by the 2 Billion Trees program in 2021) are being showcased to highlight the diversity of projects funded across Canada. From the application of traditional ecological knowledge, habitat restoration, increasing tree biodiversity in urban centres, or engaging local residents in an effort to educate people on the importance of nature-based climate solutions, these projects demonstrate that planting trees brings a wealth of benefits for all Canadians, from coast to coast.

Historical finds of Fenusa pumila

Historical finds of Adelges abietis