The Department of Fisheries and Oceans (DFO) Science Branch has designed a multispecies dive survey protocol to provide unbiased, coast wide monitoring of benthic invertebrate stocks (as may be required under the updated Fisheries Act) and associated habitat information for a suite of benthic marine invertebrate species (Green (Strongylocentrotus droebachiensis), Purple (S. purpuratus) and Red Sea Urchin (Mesocentrotus franciscanus), Geoduck (Panopea generosa), Giant Red Sea Cucumber (Apostichopus californicus), Northern Abalone (Haliotis kamtschatkana), and Sunflower Sea Star (Pycnopodia helianthoides)). Based on information available at this time, the proposed survey design can provide estimates of coast wide stock status for Red Sea Urchin and Giant Red Sea Cucumber, and relative abundance indices for Geoduck, Green Sea Urchin, Purple Sea Urchin, Northern Abalone and Sunflower Sea Star.The new protocol was tested through a series of pilot surveys conducted on a subset of areas of the BC coast each September from 2016-2021. Design of the pilot surveys was based on previous dive survey data and experience and demonstrated the practical feasibility of the protocol, while also gathering preliminary information to guide recommendations about the statistical design of the survey.The dataset consists of a relational database containing tables representing each component of the survey methodology. The primary component of the survey is a transect location. Along each transect, systematically spaced quadrats are sampled, and on each quadrat, substrate observations are recorded, multiple species of algae are recorded, and multiple individual invertebrates are measured or counted. The tables are linked by transect number and quadrat number.

Kernel density estimation (KDE) utilizes spatially explicit data to model the distribution of a variable of interest. It is a simple non-parametric neighbour-based smoothing function that relies on few assumptions about the structure of the observed data. It has been used in ecology to identify hotspots, that is, areas of relatively high biomass/abundance, and in 2010 was used by Fisheries and Oceans Canada to delineate significant concentrations of corals and sponges. The same approach has been used successfully in the Northwest Atlantic Fisheries Organization (NAFO) Regulatory Area. Here, we update the previous analyses with the catch records from up to 5 additional years of trawl survey data from Eastern Canada, including the Gulf of Saint Lawrence. We applied kernel density estimation to create a modelled biomass surface for each of sponges, small and large gorgonian corals, and sea pens, and applied an aerial expansion method to identify significant concentrations of these taxa. We compared our results to those obtained previously and provided maps of significant concentrations as well as point data co-ordinates for catches above the threshold values used to construct the significant area polygons. The borders of the polygons can be refined using knowledge of null catches and species distribution models of species presence/absence and/or biomass.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

Marine classification schemes based on abiotic surrogates often inform regional marine conservation planning in lieu of detailed biological data. However, theses chemes may poorly represent ecologically relevant biological patterns required for effective design and management strategies. We used a community-level modeling approach to characterize and delineate representative mesoscale (tens to thousands of kilometers) assemblages of demersal fish and benthic invertebrates in the North-west Atlantic. Hierarchical clustering of species occurrence data from four regional annual multispecies trawl surveys revealed three to six groupings (predominant assemblage types) in each survey region, broadly associated with geomorphic and oceanographic features. Indicator analyses identified 3–34 emblematic taxa of each assemblage type. Random forest classifications accurately predicted assemblage dis-tributions from environmental covariates (AUC > 0.95) and identified thermal limits (annual minimum and maximum bottom temperatures) as important pre-dictors of distribution in each region. Using forecasted oceanographic conditions for the year 2075 and a regional classification model, we projected assemblage dis-tributions in the southernmost bioregion (Scotian Shelf-Bay of Fundy) under ahigh emissions climate scenario (RCP 8.5). Range expansions to the north eastare projected for assemblages associated with warmer and shallower waters of the Western Scotian Shelf over the 21st century as thermal habitat on the rela-tively cooler Eastern Scotian Shelf becomes more favorable. Community-level modeling provides a biotic-informed approach for identifying broadscale ecolog-ical structure required for the design and management of ecologically coherent, representative, well-connected networks of Marine Protected Areas. When com-bined with oceanographic forecasts, this modeling approach provides a spatial tool for assessing sensitivity and resilience to climate change, which can improve conservation planning, monitoring, and adaptive management.Cite this data as: O'Brien, J.M., Stanley, R.R.E., Jeffery, N.W., Heaslip, S.W., DiBacco, C., and Wang, Z. Demersal fish and benthic invertebrate assemblages in the Northwest Atlantic.Published: December 2024. Coastal Ecosystems Science Division, Maritimes region, Fisheries and Oceans Canada, Dartmouth NS.https://open.canada.ca/data/en/dataset/14d55ea5-b17d-478c-b9ee-6a7c04439d2b

Since 1985, research surveys targeting scallops—primarily the sea scallop (Placopecten magellanicus) and, to a lesser extent, the Icelandic scallop (Chlamys islandica)—have been conducted by Fisheries and Oceans Canada (DFO) at one- or two-year intervals around the Magdalen Islands (fishing area 20A). The main objective of this survey is to assess the status of sea scallop stocks. The study area is situated south of the Magdalen Islands, where scallop beds are typically sampled at depths ranging from approximately 25 to 35 m. Sampling stations are randomly selected from a predetermined fixed grid, with sampling conducted along transects at these randomly assigned locations within the study area. Each station is sampled using a lined Digby scallop dredge (20 mm mesh size), towed for roughly 500 m across the seabed.This publication includes three files: the file biometriePetoncle_20, which contains detailed biometric data (species, size, weights and sex) from 1998 to 2024; the file taillePetoncle_20, which provides the size of the individuals sampled from 2009 to 2024; and the file traitPetoncle_20 which contains the abundances and densities per tow from 2009 to 2024. Data on abundances and densities per tow from 1998-2008 is available upon request.This dataset is updated every one to two years as data becomes available. A cleaning of aberrant data has been carried out. However, there is missing data in various columns of the dataset – use the data with caution. If you have any questions please contact DFO.DataManagementSAISB-GestionDonneesDAISS.MPO@dfo-mpo.gc.ca or the author. For certain time periods, associated species are identified and semi-quantitatively counted directly on the sorting table, and the results are presented in the following publications: - https://open.canada.ca/data/en/dataset/6529a4b0-f863-4568-ac71-1fa26cf68679- https://open.canada.ca/data/en/dataset/71732ad5-5c70-4dbf-916d-a94e1380c53b 

Fisheries and Oceans Canada (DFO) conducts an annual summer multidisciplinary scientific survey with a bottom trawl in the Estuary and the northern Gulf of St. Lawrence since 1984. Over the years this survey has been conducted on four vessels: the MV Lady Hammond (1984-1990), the CCGS Alfred Needler (1990-2005), the CCGS Teleost (2004-2021) and the CCGS Cabot (2022-current). It is important to note that the objectives, the methods used and the identification of the species during these surveys have improved over time in response to DFO requests and mandates. The data are therefore not directly comparable between these surveys. The specificities of the missions onboard the CCGS Alfred Needler are described below.Objectives:1. Assess groundfish and northern shrimp population abundance and condition2. Assess environmental conditions3. Conduct a biodiversity inventory of benthic and demersal megafauna4. Monitor the pelagic ecosystem5. Collect samples for various research projectsSurvey descriptionThe survey covers the divisions 4R, 4S, 3Pn and the northern part of division 4T of the Northwest Atlantic Fisheries Organization (NAFO). This survey follows a stratified random sampling design and the fishing gear used on the CCGS Alfred Needler is a URI shrimp trawl (81'/114'). Standard trawling tows last 25 minutes, starting from the time the trawl touched the sea floor. Towing speed is 3 knots.DataFor each fishing tow, the catch is sorted and weighed by taxa; individuals are then counted and biological data are collected on a subsample. For fish, crab and squid, size and weight are measured by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish, and the otoliths are collected for several species such as witch flounder, Atlantic cod, Greenland halibut and Atlantic halibut. A roughly 2-kg shrimp sample is sorted and weighed by species (and by stage of maturity for northern shrimp). The shrimps are measured individually. The other invertebrates are counted (no individual measurements) and photographed.The biological data are divided into 5 files: a “Metadata” file containing set information, a “Catches” file containing catches per set for fish taxa, a “Carbio” file containing biological and morphometric measurements per individual, a “Freql” file containing the length frequency of fish and a “Shrimps” file containing information on shrimp catches.It's important to note that this is raw data. Only sets considered successful are retained. In each set, all species are kept, with a few exceptions. For more information please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca).

Polygons denoting concentrations of sea pens, small and large gorgonian corals and sponges on the east coast of Canada have been identified through spatial analysis of research vessel survey by-catch data following an approach used by the Northwest Atlantic Fisheries Organization (NAFO) in the Regulatory Area (NRA) on Flemish Cap and southeast Grand Banks. Kernel density analysis was used to identify high concentrations and the area occupied by successive catch weight thresholds was used to identify aggregations. These analyses were performed for each of the five biogeographic zones of eastern Canada. The largest sea pen fields were found in the Laurentian Channel as it cuts through the Gulf of St. Lawrence, while large gorgonian coral forests were found in the Eastern Arctic and on the northern Labrador continental slope. Large ball-shaped Geodia spp. sponges were located along the continental slopes north of the Grand Banks, while on the Scotian Shelf a unique population of the large barrel-shaped sponge Vazella pourtalesi was identified. The latitude and longitude marking the positions of all tows which form these and other dense aggregations are provided along with the positions of all tows which captured black coral, a non-aggregating taxon which is long-lived and vulnerable to fishing pressures.These polygons identify sea pen fields from the broader distribution of sea pens in the Northern Gulf region as sampled by Campelen trawl gear in the Gulf biogeographic zone. A 7 kg minimum threshold for the sea pen catch was identified as the weight that separated the sea pen field habitat from the broader distribution of sea pens with these research vessel tow data and gear type.

The Northwest Atlantic Fisheries Organization (NAFO) Secretariat, Fisheries and Oceans Canada (DFO) and the Canadian Hydrographic Service (CHS) have collaborated to update the spatial representation of the NAFO Subareas, Divisions, and Subdivisions as defined in Annex 1 to the Convention on Cooperation in the Northwest Atlantic Fisheries (2020) (https://www.nafo.int/Portals/0/PDFs/key-publications/NAFOConvention.pdf). The NAFO Convention does not indicate which datum should be used for spatial representation. The datum used at the time of development of the NAFO Convention would have been North American Datum 1927 (NAD27). However, all datasets were derived using NAD83.International boundaries have been updated based on accepted coordinates between the USA and Canada (http://www.internationalboundarycommission.org/en/maps-coordinates/coordinates.php), and Canada and Greenland (https://www.treaty-accord.gc.ca/text-texte.aspx?id=105136).This version of the NAFO Divisions is not intended to be used for legal purposes and is being provided for mapping / illustrative purposes only.

Polygons denoting concentrations of sea pens, small and large gorgonian corals and sponges on the east coast of Canada have been identified through spatial analysis of research vessel survey by-catch data following an approach used by the Northwest Atlantic Fisheries Organization (NAFO) in the Regulatory Area (NRA) on Flemish Cap and southeast Grand Banks. Kernel density analysis was used to identify high concentrations and the area occupied by successive catch weight thresholds was used to identify aggregations. These analyses wereperformed for each of the five biogeographic zones of eastern Canada. The largest sea pen fields were found in the Laurentian Channel as it cuts through the Gulf of St. Lawrence, while large gorgonian coral forests were found in the Eastern Arctic and on the northern Labrador continental slope. Large ball-shaped Geodia spp. sponges were located along the continental slopes north of the Grand Banks, while on the Scotian Shelf a unique population of the large barrel-shaped sponge Vazella pourtalesi was identified. The latitude and longitude marking the positions of all tows which form these and other dense aggregations are provided along with the positions of all tows which captured black coral, a non-aggregating taxon which is long-lived and vulnerable to fishing pressures.These polygons identify small gorgonian coral fields from the broader distribution of small gorgonian corals in the region as sampled by Campelen trawl gear in the Eastern Arctic biogeographic zone. A 0.05 kg minimum threshold for the small gorgonian coral catch was identified as the weight that separated the small gorgonian field habitat from the broader distribution of small gorgoninan corals with these research vessel tow data and gear type.

Fisheries and Oceans Canada (DFO) conducts an annual summer multidisciplinary scientific survey with a bottom trawl in the Estuary and the northern Gulf of St. Lawrence since 1984. Over the years this survey has been conducted on four vessels: the MV Lady Hammond (1984-1990), the CCGS Alfred Needler (1990-2005), the CCGS Teleost (2004-2022) and the CCGS Cabot (2022-present). It is important to note that the objectives, the methods used and the identification of the species during these surveys have improved over time in response to DFO requests and mandates. The data are therefore not directly comparable between these surveys. However, comparative analyses have been carried out between vessels, and conversion factors are available on request for a number of species. The specificities of the missions onboard the CCGS Teleost are described below.Objectives:1. Assess groundfish and northern shrimp population abundance and condition2. Assess environmental conditions3. Conduct a biodiversity inventory of benthic and demersal megafauna 4. Assess phytoplankton and mesozooplankton abundance5. Monitor the pelagic ecosystem6. Inventory marine mammals populations7. Inventory seabirds populations8. Collect samples for various research projectsSurvey descriptionThe survey covers the Estuary and the northern Gulf of St. Lawrence, that is the divisions 4R, 4S and the northern part of division 4T of the Northwest Atlantic Fisheries Organization (NAFO). Since 2008, coverage of division 4T has been increased in the upstream part of the Lower Estuary in order to sample the depths between 37 and 183 m. A stratified random sampling strategy is used for this survey and the area of the study area is 118,587 km². The fishing gear used on the CCGS Teleost is a four-sided Campelen 1800 shrimp trawl equipped with a Rockhopper footgear (“bicycle”). The trawl lengthening and codend are equipped with a 12.7-mm knotless nylon lining. Standard trawling tows last 15 minutes, starting from the time the trawl touches the sea floor. The target towing speed is 3 knots.DataFor each fishing tow, the catch is sorted and weighed by taxa; individuals are then counted and biological data are collected on a subsample. For fish, crab and squid, size and weight are measured by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish, and the otoliths are sampled for several species such as Atlantic cod, Atlantic halibut, Greenland halibut, and witch flounder. A roughly 2-kg shrimp sample is sorted and weighed by species (and by stage of maturity for northern shrimp). The shrimps are measured individually. The other invertebrates are counted (no individual measurements) and photographed.The biological data are divided into 4 files: a “Metadata” file containing set information, a “Catches” file containing catches per set for fish taxa, a a “Carbio” file containing biological and morphometric measurements per individual and a “Shrimps” file containing information on shrimp catches.It's important to note that this is raw data. Only set considered successful are retained. In each set, all species are kept, with a few exceptions. For more information please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca).