Harbour seals reside throughout the year around Newfoundland and Labrador (NL). The first systematic survey for harbour seals occurred along the NL Shelf during July and August 2021 to obtain counts of hauled out individuals and assess distribution. Grey seals are seasonal residents in NL, mainly present in the summer and autumn months. Grey seals were also recorded during the survey as these two species can share haul-out locations. Surveys were flown along the coastline with a Bell 429 helicopter with photographs taken of hauled out seals. This data includes the counts of hauled out harbour, grey and unknown seals seen during the survey. Adjusted counts are also provided, which assign the unknown seals to species based on the number of positively identified harbour and grey seals from each survey day. The realized survey coverage (survey tracks) is also included. Cite this data as: Hamilton, C.D., Goulet, P.J., Stenson, G.B., and Lang, S.L.C. 2024. Data of: Counts of harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) from an aerial survey of the coast of the Newfoundland Shelf and Sandwich Bay, Labrador during the summer of 2021This data can be found in: Hamilton, C.D., Goulet, P.J., Stenson, G. B., and Lang, S.L.C. 2023. Counts and spatial distribution of harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) from an aerial survey of the coast of the Newfoundland Shelf and Sandwich Bay, Labrador during the summer of 2021. Can. Tech. Rep. Fish. Aquat. Sci. 3566: v + 39 p. https://publications.gc.ca/site/eng/9.927831/publication.html DFO. 20XX. Stock assessment of Atlantic harbour seals (Phoca vitulina vitulina) in Canada for 2019-2021. DFO Can. Sci. Advis Sec. Sci. Advis. Rep. 2023/XXX. Lang, S.L.C., St-Pierre, A.P., Hamilton, C.D., Mosnier, A., Lidgard, D.C., Goulet, P., den Heyer, C.E., Bordeleau, X., Irani, A.I., and Hammill, M.O. 20XX. Population status assessment and Potential Biological Removal (PBR) for the Atlantic harbour seal (Phoca vitulina vitulina) in Canadian waters. DFO Can. Sci. Advis. Sec. Res. Doc. 2024

Harbour seals (Phoca vitulina) are found along temperate and Arctic marine coastlines of the Northern Hemisphere. They are found in coastal waters of the northern Atlantic and Pacific Oceans, as well as those of the Baltic and North Seas. In Canada, they may be found off the coastal waters of British Columbia, Nunavut, Manitoba, Ontario, Quebec, New Brunswick, Prince Edward Island, Nova Scotia, and Newfoundland and Labrador.Population trends and abundance of harbour seals in British Columbia are assessed based on aerial surveys conducted during 1966-2019. Based on counts conducted in Index Areas distributed throughout the province, the trend observed in the Strait of Georgia appears to be generally indicative of harbour seal populations throughout British Columbia. Total abundance of harbour seals on the B.C. coast in 2008 was estimated to be on the order of about 105,000 (95% confidence interval of 90,900 to 118,900) seals. Total abundance was re-estimated in 2022 (estimate and CI pending completion of CSAS process). Historic reconstructions indicate the population was depleted by a period of commercial harvesting during 1879-1914, and subsequently maintained below natural levels by predator control programs until the early 1960s. Already depleted, the population could not sustain a second period of intense commercial harvesting during 1962-1968 and was further depleted, but now appears to have fully recovered.

The initial objective of this dataset was to study the seasonal movement patterns of harbour seals (Phoca vitulina) in the St. Lawrence Estuary and Sable Island. This study was part of a larger program that studied the foraging behavior of the species.Ten harbour seals were captured using gillnets from 1994 to 1998 at three sites in the St. Lawrence Estuary (Bic, n=1 individual; île Blanche, n=1; Métis-sur-Mer, n=5) and one site on Sable Island (n=3 individuals). The individuals were equipped with a satellite-linked time-depth recorder (Type3.10, Wildlife Computers) equipped with an Argos tag and placed on the back of the neck. For most individuals, satellite tracking began in September and continued until the following spring.The dataset consists of series of geographic locations of ten harbor seals with associated dates and times and movement speeds calculated from successive locations.The location data were only filtered based on the validity class provided by Argos. Class Z locations were excluded.

Most of the data were collected during aerial surveys carried out at low tides during June and August 1994-1997, 2000 and 2001. June and August are respectively pupping and moulting seasons, when the haulout sites are intensively used by seals. Features in this layer show the Harbour seal distribution and the mean abundance for all aerial surveys (tables 3 and 5, figures 3 and 5 from Robillard et al. 2005). In the estuary, areas of high abundance have more than 30 individuals, areas of medium abundance have between 10 and 30 individuals and areas of low abundance have fewer than 10 individuals. In the Gulf, areas of high abundance have more than 50 individuals and areas of medium to low abundance have fewer than 50 individuals. Unpublished data obtained from Parks Canada and Sepaq were also used to identify important haulout areas in the Saguenay Fjord sector and in Pointe-aux-Vaches tidal flat sectors, which have been categorized in this dataset as high abundance areas.Data are valid only during summer (except for the Pointe-aux-Vaches flats identified as mainly frequented in autumn by Parc Canada), because spring and fall distributions of the Harbour seal are unknown. Data shown in the Estuary and the Gulf of St. Lawrence are a picture of the situation in 2005 because it is the most recent mapping available for this specie. The distribution of the Harbour seal is non-uniform among the different concentration areas but is similar between June and August. However, Harbour seals tend to decrease their presence along the south shore and the Lower Estuary in August to the benefit of the Saguenay River colonies. Abundance classes are arbitrary but fit with the published results of haulout site utilization from Robillard et al. (2005).Data sources :Parks Canada. 2021. Personal communication. Harbor seal monitoring data on the Pointe-aux-Vaches tidal flat. Parks Canada and SÉPAQ, 2020. Données du suivi du phoque commun dans le fjord du Saguenay. Unpublished data.Robillard, A., V. Lesage, and M.O. Hammill. 2005. Distribution and abundance of harbour seals (Phoca vitulina concolor) and grey seals (Halichoerus grypus) in the Estuary and Gulf of St. Lawrence, 1994–2001. Can. Tech. Rep. Fish. Aquat. Sci. 2613: 152 pp.

Data were collected during aerial surveys carried out at low tides in June and August 1994-1997, 2000 and 2001. June and August are respectively pupping and moulting seasons, when the haulout sites are intensively used by seals. Features in this layer show the Grey seal distribution and mean abundance for all aerial surveys (tables 4 and 6, figures 4 and 6 from Robillard et al. 2005). In the estuary, areas of high abundance have more than 25 individuals, areas of medium abundance have between 5 and 25 individuals and areas of low abundance have fewer than 5 individuals. In the Gulf, areas of high abundance have more than 70 individuals and areas of medium to low abundance have fewer than 70 individuals.Data are valid only during summer because Grey seals in the Estuary and northern Gulf migrate to the southern Gulf of St. Lawrence in the fall. These seals will spend the winter on Sable Island, on the ice shelf in the Northumberland Strait or on neighboring islands. During the summer, in the Estuary and the Gulf of St. Lawrence, its distribution is not uniform between the different concentration areas identified, but it is similar between June and August. However, there are some areas where Grey seals are more abundant in August than in June. Abundance classes are arbitrary but fit with the published results of haul-out sites utilization from Robillard et al. (2005). Data shown are a picture of the situation in 2005 because it is the most recent mapping available for this species.Data sources and references:Lavigueur, L., Hammill, M.O., and Asselin, S. 1993. Distribution et biologie des phoques et autres mammifères marins dans la région du parc marin du Saguenay. Rapp. manus. can. sci. halieut. aquat. 2220: vi + 40.Lesage, V., and Hammill, M.O. 2001. The status of the grey seal, Halichoerus grypus, in the Northwest Atlantic. Can. Field-Nat. 115(4): 653-662.Robillard, A., V. Lesage, and M.O. Hammill. 2005. Distribution and abundance of harbour seals (Phoca vitulina concolor) and grey seals (Halichoerus grypus) in the Estuary and Gulf of St. Lawrence, 1994–2001. Can. Tech. Rep. Fish. Aquat. Sci. 2613: 152 pp.

Layers that present various important parameters such as inventories, presence, sightings, distribution, relative occurrence or catch rates, critical habitat, breeding and feeding areas, potential spawning and haul-out sites for the different species with status under the Species at Risk Act (SARA).The act classifies those species as being either extirpated, endangered, threatened, or of special concern. Under SARA, Fisheries and Oceans Canada must produce recovery strategies and action plans for aquatic species listed as endangered or threatened. The act is part of Canada’s strategy to protect hundreds of wild plants and animal species from becoming extinct, and to help in their recovery.The different species represented by the layers are the following:1. American shad (Alosa sapidissima)2. Atlantic sturgeon (Acipenser oxyrinchus)3. Atlantic wolffish (Anarhichas lupus)4. Beluga whale (Delphinapterus leucas)5. Blue whale (Balaenoptera musculus)6. Copper redhorse (Moxostoma hubbsi)7. Eelgrass (Zostera marina)8. Grey seal (Halichoerus grypus)9. Harbor seal (Phoca vitulina)10. Humpback whale (Megaptera novaeangliae)11. Lake sturgeon (Acipenser fulvescens)12. Lumpfish (Cyclopterus lumpus)13. Northern wolffish (Anarhichas denticulatus)14. Rainbow smelt (Osmerus mordax)15. Sea pens (Pennatulacea)16. Seaweed17. Smooth skate (Malacoraja senta)18. Sponges19. Spotted wolffish (Anarhichas minor)20. Striped bass (Morone saxatilis)21. Thorny skate (Amblyraja radiata)22. Winter skate (Leucoraja ocellata)

Layer that includes the known information on potential haul-out sites for the harbour seal and gray seal in the Estuary and Gulf of St. Lawrence according to a literature review of documents produced between 1978 and 2000.Additional InformationPotential haul-out sites for the harbor seal and gray seal were produced according to a literature review of the following documents:Andersen, A. et M. Gagnon. 1980. Les ressources halieutiques de l'estuaire du Saint-Laurent. Rapp. can. ind. sci. halieut. aquat., 119: iv + 56 p.Argus Groupe-Conseil inc. 1992. Synthèse et analyse des connaissances relatives aux ressources naturelles du Saguenay et de l'estuaire maritime du Saint-Laurent. Parc marin du Saguenay. Service canadien des parcs, région du Québec.Biorex. 1995. Cartographie des ressources halieutiques et de leurs habitats dans l'estuaire moyen du Saint-Laurent. Rapport au ministère des Pêches et des Océans, Région du Québec, Division de la gestion de l'habitat du poisson. 36 p. + annexes. Biorex. 1996. Base de données géoréférencées sur les ressources halieutiques et leurs habitats : estuaire maritime du Saint-Laurent et fjord du Saguenay. Rapport au ministère des Pêches et des Océans, Région du Québec, Division de la gestion de l'habitat du poisson. Volume 1 : 38 p. + annexes et Volume 2 : 34 p. + annexes.Chevrier, V. 1994. Cartographie des habitats du poisson aux Îles-de-la-Madeleine. Rapport technique no. 1. Réalisé en collaboration avec le M.P.O., Attention Frag'Îles et la M.R.C. des Îles-de-la-Madeleine. 24 p. + 1 ann. + 36 cartes.Comité de la zone d'intervention prioritaire (ZIP) de la Côte-Nord du golfe. 1999. Inventaire des habitats côtiers et marins sensibles de la Basse-Cote-Nord : Rapport final, présenté à Pêches et Océans Canada par le Comité ZIP Côte-Nord du Golfe. 126 p.Comité de protection de la santé et de l'environnement de Gaspé inc. (C.P.S.E.G.). 1996.Communication personnelle par Carol Fournier, MPO. 1999. Communications personnelles par Gosselin, J-F-. 1996. Desaulniers, J. 1989. Étude des populations de pinnipèdes de l'Archipel-de-Mingan et relation entre l'activité de chasse au phoque et la sécurité publique 1987 à 1989. Parcs Canada. Région du Québec. Service de la conservation des ressources naturelles.Enquêtes auprès des pêcheurs. 1995.Lavigne, P.-J. 1978. La chasse estivale du phoque dans le Saint-Laurent. Rapport non publié. 65 p. Pêches et Océans Canada, Région du Québec.Lesage, Véronique. 2000. Communication personnel.Lesage, V., M. O. Hammill, and K.M. Kovacs. 1995. Harbour seal (Phoca vitulina) and Grey seal (Halichoerus grypus) abundance in the St. Lawrence estuary. Can. Manuscr. Rep. Fish. Aquat. Sci. 2307: iii + 19 p.Naturam Environnement inc. 1996. Caractérisation physique et biologique de l'habitat du poisson du secteur de Pointe-aux-Outardes. Realisé pour le compte de la Corporation du Parc régional de Pointe-aux-Outardes. 196 p.

Phytoplankton pigments, determined by high-performance liquid chromatography (HPLC) are measured on DFO cruises three times a year in February, June, and August/September along Line P in the northeast subarctic Pacific. Sampling for phytoplankton pigments started in 2006 at the five main Line P stations and was expanded to sample at all twenty seven stations along the transect in June 2010.

Phytoplankton pigments, determined by high-performance liquid chromatography (HPLC) have been measured on DFO La Perouse cruises since 2011. Surface samples are taken along a series of transects off the west coast of Vancouver Island twice a year usually in May/June and early September.

Cold-water corals are conspicuous in the waters off Eastern Canada. Despite that, there are few DNA sequence records from specimens collected in the region available in GenBank, and not all species recorded in the region have sequence data regardless of geographic origin. This can limit the use of eDNA techniques to detect and identify corals. Our objective was to sequence and publish sequences for two octocoral DNA barcoding markers: CO1 and MutS. We sequenced and deposited 36 sequences to GenBank from 19 specimens representing three sea pen taxa (Octocorallia: Pennatuloidea): Distichoptilum gracile, Pennatula aculeata, and Protoptilum carpenteri. Identification of all specimens was confirmed by B. M. Neves before submission. Specimens and DNA tissues were donated to the Canadian Museum of Nature, where they are currently stored. This publication is part 1 of a series of GenBank submissions by our lab.Specimens were collected from across the Northwest Atlantic and originate from depths ranging between 200-1924 meters. Specimens were collected as part of research vessel multispecies trawl surveys or remotely operated vehicle (ROV ROPOS) surveys. DNA was isolated and purified using the QIAgen DNeasy Blood and Tissue kit, with an initial overnight incubation with Proteinase K. Two commonly used octocoral barcoding regions were amplified using previously described primers: 1) COII8068F (McFadden et al., 2004) and COIOCTR (France and Hoover, 2002) for the CO1 gene, and 2) ND42599F (France and Hoover, 2002) and mut3458R (Sánchez et al., 2003) for the MutS gene. Amplifications were conducted using 12.5 µl of Green DreamTaq Master Mix (Thermo Fisher Scientific), 1 µl of template DNA, 0.5 µl of each 10 µM forward and reverse primers, 0.5 µl of 10 µM reverse primer, and 10.5 µl of water. Thermocycling was run as follows: 3 min of initial denaturation at 95 °C, followed by 40 cycles at 95 °C for 30 s, 30 s at annealing temperature of 48 °C, then 65 s at an extension temperature of 72 °C, and a final elongation at 72 °C for 4 min. PCR products were cleaned using Agencourt AMPure XP Beads (Beckman Coulter) and sent to The Center for Advanced Genomics, Toronto, Canada for Sanger sequencing. Sequences were visualized and aligned using Geneious Prime 2022.0.2. Obtained sequences have been deposited in GenBank under accession numbers OQ569768- OQ569784 and OQ420359- OQ420377. This work was funded by Fisheries and Oceans Canada under an Enhanced Regional Capacity grant (2020-2021) and the Marine Conservation Targets (MCT) program (2021-2024), Newfoundland and Labrador Region.