Bivalve aquaculture has direct and indirect effects on plankton communities, which are highly sensitive to short-term (seasonal, interannual) and long-term climate changes, although how these dynamics alter aquaculture ecosystem interactions is poorly understood. Here, we investigate seasonal patterns in plankton abundance and community structure spanning several size fractions from 0.2 µm up to 5 mm, in a deep aquaculture embayment in northeast Newfoundland, Canada. Using flow cytometry and FlowCam imaging, we observed a clear seasonal relationship between fraction sizes driven by water column stratification (freshwater input, nutrient availability, light availability, water temperature). Plankton abundance decreased proportionally with increasing size fraction, aligning with size spectra theory. Within the bay, greater mesozooplankton abundance, and a greater relative abundance of copepods, was observed closest to the aquaculture lease. No significant spatial effect was observed for phytoplankton composition. While the months of August to October showed statistically similar plankton composition and size spectra slopes (i.e., food chain efficiency) and could be used for interannual variability comparisons of plankton composition, sampling for longer periods could capture long-term phenological shifts in plankton abundance and composition related to various processes, including climate change. Conclusions provide guidance on optimal sampling to monitor and assess aquaculture pathways of effects.Cite this data as: Sharpe H, Lacoursière-Roussel A, Gallardi D (2024). Ecological insight of seasonal plankton succession to monitor shellfish aquaculture ecosystem interactions. Version 3.2. Fisheries and Oceans Canada. Sampling event dataset. https://doi.org/10.25607/2ujdvh

Plankton (zooplankton and large phytoplankton) are collected using the Continuous Plankton Recorder (CPR) in the Northwest (NW) Atlantic along tracks transited by container ships from Reykjavik (Iceland) to St. John’s, NL (the Z line), and between St. John’s and the New England Coast, along the Scotian Shelf (the E and MD lines). The CPR Survey is the longest running, most geographically extensive marine ecological survey in the world, providing comparable data on the geographical distribution, seasonal cycles and year-to-year changes in abundance of plankton over a large spatial area. The first northwest Atlantic samples were collected in the Irminger Sea in 1957, and sampling was extended farther west to the Scotian Shelf a few years later. Sampling has continued to the present with some interruptions during the late 1970s and 1980s. Sampling is nominally once per month along the E, MD, and Z lines. DFO Sample collection and analysis are led by the Continuous Plankton Recorder Survey program at the Marine Biological Association of the UK. DFO provides partial support for the northwest Atlantic survey carried out on the E, MD, and Z lines and incorporates CPR data in Atlantic Zone Monitoring Program ocean environmental status reporting.

Zooplankton and ichthyoplankton data are archived in the Institute of Ocean Sciences (IOS) Zooplankton Database. The data available spans from 1980 to 2018 and is an extraction of vertical net hauls as biomass by major taxa collected during surveys conducted in the oceanic and coastal waters of the Northeast Pacific Ocean. The majority of vertical net hauls in this data set were collected from 10 metres above the sea floor or an approximate maximum depth of 250 metres. For further data requests, please use the contact information provided.

From August 2nd to September 9th, 2013, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 64 stations along ten transects. Standardized sampling was conducted on the transects at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. A specialized CTD probe (UCTD) was deployed at an additional 72 locations while the ship was underway.Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

From August 5th to September 3rd, 2012, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 28 stations along four transects. Standardized sampling was conducted at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. A specialized CTD probe (UCTD) was deployed at an additional 30 locations while the ship was underway. Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

Bay-scale empirical demonstrations of how bivalve aquaculture alters plankton composition, and subsequently ecological functioning and higher trophic levels, are lacking. Temporal, inter- and within-bay variation in hydrodynamic, environmental, and aquaculture pressure limit efficient plankton monitoring design to detect bay-scale changes and inform aquaculture ecosystem interactions. Here, we used flow cytometry to investigate spatio-temporal variations in bacteria and phytoplankton (< 20 µm) composition in four bivalve aquaculture embayments. We observed higher abundances of bacteria and phytoplankton in shallow embayments that experienced greater freshwater and nutrient inputs. Depleted nutrient conditions may have led to the dominance of picophytoplankton cells, which showed strong within-bay variation as a function of riverine vs freshwater influence and nutrient availability. Although environmental forcings appeared to be a strong driver of spatio-temporal trends, results showed that bivalve aquaculture may reduce near-lease phytoplankton abundance and favor bacterial growth. We discuss aquaculture pathways of effects such as grazing, benthic-pelagic coupling processes, and microbial biogeochemical cycling. Conclusions provide guidance on optimal sampling considerations using flow cytometry in aquaculture sites based on embayment geomorphology and hydrodynamics.Cite this data as: Sharpe H, Lacoursière-Roussel A, Barrell J (2024). Monitoring bay-scale bivalve aquaculture ecosystem interactions using flow cytometry. Version 1.2. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=monitoring_bay-scale_bivalve_aquaculture_ecosystem_interactions_using_flow_cytometry&v=1.2

As part of the development of a nationally-consistent sampling design within the Aquaculture Monitoring Program (AMP), this data reports mesozooplankton assemblages observed at nine coastal shellfish aquaculture sites, located across four DFO regions, with sampling across months, tide phases, and sampling locations. In most sites, strong spatial effects were observed, while tide effects were generally less important for structuring the mesozooplankton communities. Seasonality emerged as an essential factor to design an efficient monitoring program. This dataset represents the first large-scale Canadian coastal study using imaging technology for plankton taxonomic Identification.Cite this data as: Finnis, S., Guyondet, T., McKindsey, C.W., Arseneau, J., Barrell, J., Duhaime, J., Filgueira, R., Gallardi, D., Gaspard, D., Gibb, O., Goodwin, C., Hua, K., Macdonald, T., Milne, R., Lacoursière-Roussel, A. 2023. Guidance on sampling effort to monitor mesozooplankton communities at Canadian bivalve aquaculture sites using an optical imaging system. Can. Tech. Rep. Fish. Aquat. Sci. 3581: vii + 101 p

Surficial geologic units are unconsolidated materials overlying bedrock. The dataset represents a general description of the stratigraphy and geology, including geologic unit thickness, morphology, age and rank. It features a list of the geologic unit names and types of sediment in the hydrogeological unit. While the preferred format to deliver this data is by using a shapefile and its linked attributes, this dataset can be delivered also by providing link to external data which should have at least the same properties or also by joining a georeferenced image of the map.

The bedrock geologic units designate units of the same types of rock which composed the solid rock exposed at ground surface (as outcrop) or which underlies unconsolidated surficial sediments. This dataset represents a general description of the stratigraphy and geology, including geologic unit thickness, morphology, age and rank. It features a list of the geologic unit names and types of rock (lithology) in the hydrogeological unit, from a controlled vocabulary. While the preferred format to deliver this data is by using a shapefile and its linked attributes, this dataset can be delivered also by providing link to external data which should have at least the same properties or also by joining a georeferenced image of the map.

Snow survey administrative basin areas, which are components of the BC snow survey network. Basin codes are used as basis of snow survey station names, and for some reporting purposes.