Bivalve aquaculture has direct and indirect effects on plankton communities, which are highly sensitive to short-term (seasonal, interannual) and long-term climate changes, although how these dynamics alter aquaculture ecosystem interactions is poorly understood. Here, we investigate seasonal patterns in plankton abundance and community structure spanning several size fractions from 0.2 µm up to 5 mm, in a deep aquaculture embayment in northeast Newfoundland, Canada. Using flow cytometry and FlowCam imaging, we observed a clear seasonal relationship between fraction sizes driven by water column stratification (freshwater input, nutrient availability, light availability, water temperature). Plankton abundance decreased proportionally with increasing size fraction, aligning with size spectra theory. Within the bay, greater mesozooplankton abundance, and a greater relative abundance of copepods, was observed closest to the aquaculture lease. No significant spatial effect was observed for phytoplankton composition. While the months of August to October showed statistically similar plankton composition and size spectra slopes (i.e., food chain efficiency) and could be used for interannual variability comparisons of plankton composition, sampling for longer periods could capture long-term phenological shifts in plankton abundance and composition related to various processes, including climate change. Conclusions provide guidance on optimal sampling to monitor and assess aquaculture pathways of effects.Cite this data as: Sharpe H, Lacoursière-Roussel A, Gallardi D (2024). Ecological insight of seasonal plankton succession to monitor shellfish aquaculture ecosystem interactions. Version 3.2. Fisheries and Oceans Canada. Sampling event dataset. https://doi.org/10.25607/2ujdvh

Plankton (zooplankton and large phytoplankton) are collected using the Continuous Plankton Recorder (CPR) in the Northwest (NW) Atlantic along tracks transited by container ships from Reykjavik (Iceland) to St. John’s, NL (the Z line), and between St. John’s and the New England Coast, along the Scotian Shelf (the E and MD lines). The CPR Survey is the longest running, most geographically extensive marine ecological survey in the world, providing comparable data on the geographical distribution, seasonal cycles and year-to-year changes in abundance of plankton over a large spatial area. The first northwest Atlantic samples were collected in the Irminger Sea in 1957, and sampling was extended farther west to the Scotian Shelf a few years later. Sampling has continued to the present with some interruptions during the late 1970s and 1980s. Sampling is nominally once per month along the E, MD, and Z lines. DFO Sample collection and analysis are led by the Continuous Plankton Recorder Survey program at the Marine Biological Association of the UK. DFO provides partial support for the northwest Atlantic survey carried out on the E, MD, and Z lines and incorporates CPR data in Atlantic Zone Monitoring Program ocean environmental status reporting.

The objective of this project was to gather data to develop a model of the food web of the lower trophic levels of the nearshore area of the Beaufort Sea. Sampling took place from 2005 to 2008 using the CCGS Nahidik. The multidisciplinary character of the Nahidik program produced measurements of biology/ecology (primary production, phytoplankton, zooplankton, benthos, fish), chemical and physical oceanography, contaminants, geology and hydro acoustics. The data were collected in July and August of each year. The Nahidik program provided data to provide a baseline for future studies as well as an information source for environmental assessment.This record contains water chemistry data collected as part of this project including suspended nitrogen, dissolved nitrogen, suspended phosphorus, dissolved phosphorus, dissolved organic carbon, suspended carbon, chlorophyll a, and suspended silicon.

The objective of this project was to gather data to develop a model of the food web of the lower trophic levels of the nearshore area of the Beaufort Sea. Sampling took place from 2005 to 2008 using the CCGS Nahidik. The multidisciplinary character of the Nahidik program produced measurements of biology/ecology (primary production, phytoplankton, zooplankton, benthos, fish), chemical and physical oceanography, contaminants, geology and hydro acoustics. The data were collected in July and August of each year. The Nahidik program provided data to provide a baseline for future studies as well as an information source for environmental assessment. This record contains the geographic coordinates and station names from 2005 to 2008.

This record contains information on the samples collected and the intended parameters for analysis from sediment cores collected as part of this project including bacteria, chemistry, stable isotopes, fatty acids, or meiofauna.

Bay-scale empirical demonstrations of how bivalve aquaculture alters plankton composition, and subsequently ecological functioning and higher trophic levels, are lacking. Temporal, inter- and within-bay variation in hydrodynamic, environmental, and aquaculture pressure limit efficient plankton monitoring design to detect bay-scale changes and inform aquaculture ecosystem interactions. Here, we used flow cytometry to investigate spatio-temporal variations in bacteria and phytoplankton (< 20 µm) composition in four bivalve aquaculture embayments. We observed higher abundances of bacteria and phytoplankton in shallow embayments that experienced greater freshwater and nutrient inputs. Depleted nutrient conditions may have led to the dominance of picophytoplankton cells, which showed strong within-bay variation as a function of riverine vs freshwater influence and nutrient availability. Although environmental forcings appeared to be a strong driver of spatio-temporal trends, results showed that bivalve aquaculture may reduce near-lease phytoplankton abundance and favor bacterial growth. We discuss aquaculture pathways of effects such as grazing, benthic-pelagic coupling processes, and microbial biogeochemical cycling. Conclusions provide guidance on optimal sampling considerations using flow cytometry in aquaculture sites based on embayment geomorphology and hydrodynamics.Cite this data as: Sharpe H, Lacoursière-Roussel A, Barrell J (2024). Monitoring bay-scale bivalve aquaculture ecosystem interactions using flow cytometry. Version 1.2. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=monitoring_bay-scale_bivalve_aquaculture_ecosystem_interactions_using_flow_cytometry&v=1.2

From August 5th to September 3rd, 2012, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 28 stations along four transects. Standardized sampling was conducted at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. A specialized CTD probe (UCTD) was deployed at an additional 30 locations while the ship was underway. Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

From August 2nd to September 9th, 2013, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 64 stations along ten transects. Standardized sampling was conducted on the transects at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. A specialized CTD probe (UCTD) was deployed at an additional 72 locations while the ship was underway.Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

The objective of this project was to gather data to develop a model of the food web of the lower trophic levels of the nearshore area of the Beaufort Sea. Sampling took place from 2005 to 2008 using the CCGS Nahidik. The multidisciplinary character of the Nahidik program produced measurements of biology/ecology (primary production, phytoplankton, zooplankton, benthos, fish), chemical and physical oceanography, contaminants, geology and hydro acoustics. The data were collected in July and August of each year. The Nahidik program provided data to provide a baseline for future studies as well as an information source for environmental assessment.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a