PURPOSE:The purpose of this work was to determine (1) how the population genomic structure relates to gut microbiota composition of White Hake (Urophycis tenuis), and (2) whether microbiota community variation provides complementary insights into population structure of this species in eastern Canada.DESCRIPTION:Integrating host-associated microbiota with genomic approaches offers an opportunity to better understand the multiple biological dimensions shaping population structure in marine fishes. A clear understanding of population structure and dynamics is essential for informed fisheries management and conservation decisions; however, while genomic approaches have greatly improved our ability to delineate biological populations, they provide only a partial representation of biological structure, as patterns of differentiation reflect both historical divergence and contemporary ecological conditions. Host-associated microbiota can influence population-level ecological processes by contributing functional, potentially heritable variation that shapes host phenotype and fitness.In this study, we combined genotyping-by-sequencing with 16S rRNA gene amplicon sequencing to examine how population genomic structure relates to gut microbiota composition and to evaluate whether microbiota community variation provides complementary insights into population structure in White Hake (Urophycis tenuis) in eastern Canada. Genomic analyses identified two populations with greater spatial overlap than previously reported. Variation partitioning revealed that host genetics explained a negligible proportion of microbiota variation compared to environmental factors and fish length, suggesting that ontogenetic shifts in habitat use and resource acquisition influence gut microbiota composition. Several taxa were differentially abundant among fish length categories used as a proxy for diet, including taxa with chitin-degrading potential such as Photobacterium and Lachnospirales, which were enriched in smaller fish known to consume a crustacean-dominated diet. Together, these results indicate that gut microbiota composition in White Hake primarily reflects ecological and life-history processes rather than host population structure. PARAMETERS COLLECTED:Environmental parameters were also collected at most sampling sites, including depth, water temperature, oxygen and salinity levels.PHYSICAL SAMPLE DETAILS:Fin samples were collected to characterize the fish genotypes. Intestine (rectum) samples were collected to study fish gut microbiome.SAMPLING METHODS:In 2022 and 2023, White Hake were sampled during Fisheries and Oceans Canada (DFO) annual bottom trawl ecosystem monitoring surveys.USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

The National Ecological Framework for Canada's "Soil Texture by Ecoprovince” dataset contains tables that provide soil texture information within the ecoprovince framework polygon. It provides soil texture codes and their English and French language descriptions as well as the percentage of the polygon that the component occupies.Soil texture indicates the relative proportions of the various soil separates (sand, silt, clay) as described by classes of texture. Soil separates are mineral particles, 2.0 mm in diameter and include: gravel 0.2 -7.5 cm and cobbles 7.5-25.0 cm. There are 12 texture group classes definitions and one class definition for Not Applicable (which indicates, for example, water, ice or urban areas).

Historical finds of Coleophora serratella

This map illustrates the distribution of soil parent material textures in the agricultural region of Alberta. Soil texture is defined by the relative proportions of the sand, silt and clay particles present. Soil textures are identified by classes using the Soil Texture Triangle illustrated below. The Soil Texture Triangle identifies the textural class of a soil at the intersection of the percent sand (x-axis) and the percent clay (y-axis). The percent silt of the soil is the remainder to add up to 100 percent. This resource was created in 2002 using ArcGIS.

Forest encroachment onto grasslands

The Canadian Aquatic Biomonitoring Network (CABIN) is an aquatic biomonitoring program for assessing the health of fresh water ecosystems in Canada. Benthic macroinvertebrates are collected at a site location and their counts are used as an indicator of the health of that water body. CABIN is based on the network of networks approach that promotes inter-agency collaboration and data-sharing to achieve consistent and comparable reporting on fresh water quality and aquatic ecosystem conditions in Canada. The program is maintained by Environment and Climate Change Canada (ECCC) to support the collection, assessment, reporting and distribution of biological monitoring information. A set of nationally standardized CABIN protocols are used for field collection, laboratory work, and analysis of biological monitoring data. A training program is available to certify participants in the standard protocols. There are two types of sites in the CABIN database (reference and test). Reference sites represent habitats that are closest to “natural” before any human impact. The data from reference sites are used to create reference models that CABIN partners use to evaluate their test sites in an approach known as the Reference Condition Approach (RCA). Using the RCA models, CABIN partners match their test sites to groups of reference sites on similar habitats and compare the observed macroinvertebrate communities. The extent of the differences between the test site communities and the reference site communities allows CABIN partners to estimate the severity of the impacts at those locations. CABIN samples have been collected since 1987 and are organized in the database by study (partner project). The data is delineated by the 11 major drainage areas (MDA) found in Canada and each one has a corresponding study, habitat and benthic invertebrate data file. Links to auxiliary water quality data are provided when available. Visits may be conducted at the same location over time with repeat site visits being identified by identical study name / site code with different dates. All data collected by the federal government is available on Open Data and more partners are adding their data continually. The csv files are updated monthly. Contact the CABIN study authority to request permission to access non open data.

The “Agricultural Major Land Practices Groups of the Canadian Prairies” dataset lays out the areas of the 5 Major Land Practices Groups of the agricultural portions of the Canadian Prairies. They are represented by vector polygons amalgamated (dissolved) from the Version 1.9 SLC polygons sharing common water resources, land use and farming practices as developed in the “Agricultural Land Practices Groups of the Canadian Prairies by SLC Polygon” of this series. The dataset is based upon selected attributes from the Soil Landscapes of Canada (SLC) and the 1996 Census of Agriculture.Typical attributes including: land in pasture, land in summerfallow, crop mixture, farm size and the level of chemical and fertilizer inputs.

The “Agricultural Land Practices Groups of the Canadian Prairies” dataset lays out the areas of the 13 Land Practices Groups of the agricultural portions of the Canadian Prairies. They are represented by vector polygons amalgamated (dissolved) from the Version 1.9 SLC polygons sharing common water resources, land use and farming practices as developed in the “Agricultural Land Practices Groups of the Canadian Prairies by SLC Polygon” of this series. The dataset is based upon selected attributes from the Soil Landscapes of Canada (SLC) and the 1996 Census of Agriculture.Typical attributes including: land in pasture, land in summerfallow, crop mixture, farm size and the level of chemical and fertilizer inputs.

The National Ecological Framework for Canada's "Soil Texture by Ecozone” dataset contains tables that provide soil texture information within the ecozone framework polygon. It provides soil texture codes and their English and French language descriptions as well as the percentage of the polygon that the component occupies.Soil texture indicates the relative proportions of the various soil separates (sand, silt, clay) as described by classes of texture. Soil separates are mineral particles, 2.0 mm in diameter and include: gravel 0.2 -7.5 cm and cobbles 7.5-25.0 cm. There are 12 texture group classes definitions and one class definition for Not Applicable (which indicates, for example, water, ice or urban areas).

The National Ecological Framework for Canada's "Soil Texture by Ecodistrict” dataset contains tables that provide soil texture information within the ecodistrict framework polygon. It provides soil texture codes and their English and French language descriptions as well as the percentage of the polygon that the component occupies.Soil texture indicates the relative proportions of the various soil separates (sand, silt, clay) as described by classes of texture. Soil separates are mineral particles, 2.0 mm in diameter and include: gravel 0.2 -7.5 cm and cobbles 7.5-25.0 cm. There are 12 texture group classes definitions and one class definition for Not Applicable (which indicates, for example, water, ice or urban areas).