This theme includes the drainage areas of various watercourse monitoring stations (physicochemical and bacteriological, benthic organisms, diatoms, pesticides, etc.) carried out by the Ministry of the Environment, the Fight against Climate Change, Wildlife and Parks (MELCCFP) as well as lake catchments (MELCCFP) as well as lake catchments including the majority of lakes in the Voluntary Lake Monitoring Network (RSVL).The drainage area and the watershed represent the territory whose water flows to the sampling station or to the outlet of the lake. Boundaries are generated using a geographic information system (GIS) from topographic maps, numerical elevation models and flow models, and watershed boundaries produced by the Main Directorate of Water Expertise (DPEH).The drainage area and watershed are used to calculate the area drained upstream of the sampling station or lake, to characterize the drained territory (for example, to determine land use), and to meet specific mapping needs. The linked tables also provide compilations of land use according to three classifications to contextualize the various monitoring carried out at the stations. Note that the use of land outside Quebec, drainage areas and transboundary watersheds is not calculated and that the percentages in each category correspond to the Quebec area only.**This third party metadata element was translated using an automated translation tool (Amazon Translate).**

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of invertebrates species whose DNA has been detected in water samples collected at 2018 using the marker COI.The surveys were carried out in the summer of 2018 from August 11 to 14, between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 9-52 meters depth in 40 stations with one sample par station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissue extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. Libraries at the COI locus were prepared by Genome Quebec and sequenced on an Illumina MiSeq PE250 system. The bioinformatics analysis of the sequences obtained was carried out using an in-house analysis pipeline as reported in Bourret et al. 2022. A first step made it possible to obtain a molecular operational taxonomic unit table (MOTU) using the cutadapt software for the removal of the adapters and the DADA2 R package for the filtration, fusion, chimera removal and data compilation. The MOTUs table was subsequently corrected by taking into account the negative controls, where the number of observations in the latter was removed from the linked samples. Singleton MOTUs have also been removed. Finally, the taxonomic assignments were carried out on the MOTUs using the IDTAXA classifier (present in the DECIPHIER R package) using a training set trained on the COI reference bank for Golf St-Laurent (GSL-rl v1.0, https://github.com/GenomicsMLI-DFO/MLI_GSL-rl) and a threshold of 40. Detections with an “Unreliable due to gaps” category were reported at the genus level only.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data are also available on SLGO platform : https://doi.org/10.26071/ogsl-cd4c205b-f63b

AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas.Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador.Reference:TunicatesVase tunicate (Ciona intestinalis) 2012 The Vase tunicate, is a high impact solitary invader and was first detected by DFO in 2012 on the Burin Peninsula at Ship Cove and Little Bay, Placentia Bay. Various mitigation measures (McKenzie et al. 2016b) contained this invasive tunicate to a small area for six years within Placentia Bay. First detected in Fortune Bay as an established population in 2019, increasing reports of the Vase tunicate have been made along the south coast of Newfoundland. The data provided here indicates the detections of this AIS in coastal NL.From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

A chlorophyll fluorescence time series was collected at various locations around the coast of Vancouver Island, British Columbia, Canada for monitoring phytoplankton concentrations. A Wetlabs ECO fluorometer was deployed every few months on a schedule depending on season and sensor availability. The instrument hung by a chain attached to the side of the buoy, or dock, depending on location, and measured chlorophyll using the fluorescence emission at 695nm. The instrument also measured turbidity by detecting the scattered light at 700nm. The units had internal batteries and data storage and were programmed to make a group of 5 measurements every 30 minutes. A copper wiper covered the sampling window between groups of measurements to reduce fouling. Times are in UTC unless otherwise stated.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

These data sets provide information pertaining to shrimp and bycatch estimates associated with otter-trawling and trapping (November, 2000) and beam-trawling (February, 2001) in Simoom Sound. Data sets were compiled and formatted by Meagan Mak.Abstract from report:As part of a project investigating possible modification of marine ecosystems by shrimp trawling and trapping, we obtained information on catches offish, shrimp, prawns , and bycatch organisms as well as weight, sex ratios , egg location and colIateral damage to several species of pandalids and eualids. Focusing on the humpback shrimp (Pandalus hypsinotus), we assessed damage to the rostrum, carapace, abdomen, and tail fan on specimens caught by beam trawling, otter trawling, and trapping. Data are given from a preliminary study conducted in Northumberland Channel in June 2000 and more comprehensive sampling from Simoom Sound in November 2000 and February 2001.

A literature review, focusing on oil sand products (e.g., diluted bitumen), diluents, spill-treating agents, and crude oil toxicology and ecological studies, relevant to the northeast Pacific was compiled as part of the Government of Canada’s World Class Tanker Safety program. Of the 763 references identified, 14 involved diluted bitumen and other heavy crude oils, indicating the need for further research of these products in the marine environment. Diluent research suggests relatively fast evaporation and dispersion times for this component, however high toxicities may pose a threat to marine biota. Historical studies indicate older dispersant formulations had potential ecological implications, therefore newer formulations, which have not been studied in detail, require full assessment. Consistent utilization of toxicology standards remains elusive, hindering species sensitivity analyses. Exxon Valdez literature demonstrates highly variable impacts from a single oil type and the need for baseline data, recovery status, and suitable ecological end-point determination.

Historical finds of Coleophora laricella

Zones were created to manage the potential spread of fish pathogens. This product requires the use of geographic information system (GIS) software.

PURPOSE:The purpose of this work was to determine (1) how the population genomic structure relates to gut microbiota composition of White Hake (Urophycis tenuis), and (2) whether microbiota community variation provides complementary insights into population structure of this species in eastern Canada.DESCRIPTION:Integrating host-associated microbiota with genomic approaches offers an opportunity to better understand the multiple biological dimensions shaping population structure in marine fishes. A clear understanding of population structure and dynamics is essential for informed fisheries management and conservation decisions; however, while genomic approaches have greatly improved our ability to delineate biological populations, they provide only a partial representation of biological structure, as patterns of differentiation reflect both historical divergence and contemporary ecological conditions. Host-associated microbiota can influence population-level ecological processes by contributing functional, potentially heritable variation that shapes host phenotype and fitness.In this study, we combined genotyping-by-sequencing with 16S rRNA gene amplicon sequencing to examine how population genomic structure relates to gut microbiota composition and to evaluate whether microbiota community variation provides complementary insights into population structure in White Hake (Urophycis tenuis) in eastern Canada. Genomic analyses identified two populations with greater spatial overlap than previously reported. Variation partitioning revealed that host genetics explained a negligible proportion of microbiota variation compared to environmental factors and fish length, suggesting that ontogenetic shifts in habitat use and resource acquisition influence gut microbiota composition. Several taxa were differentially abundant among fish length categories used as a proxy for diet, including taxa with chitin-degrading potential such as Photobacterium and Lachnospirales, which were enriched in smaller fish known to consume a crustacean-dominated diet. Together, these results indicate that gut microbiota composition in White Hake primarily reflects ecological and life-history processes rather than host population structure. PARAMETERS COLLECTED:Environmental parameters were also collected at most sampling sites, including depth, water temperature, oxygen and salinity levels.PHYSICAL SAMPLE DETAILS:Fin samples were collected to characterize the fish genotypes. Intestine (rectum) samples were collected to study fish gut microbiome.SAMPLING METHODS:In 2022 and 2023, White Hake were sampled during Fisheries and Oceans Canada (DFO) annual bottom trawl ecosystem monitoring surveys.USE LIMITATION:To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.