Bivalve aquaculture has direct and indirect effects on plankton communities, which are highly sensitive to short-term (seasonal, interannual) and long-term climate changes, although how these dynamics alter aquaculture ecosystem interactions is poorly understood. Here, we investigate seasonal patterns in plankton abundance and community structure spanning several size fractions from 0.2 µm up to 5 mm, in a deep aquaculture embayment in northeast Newfoundland, Canada. Using flow cytometry and FlowCam imaging, we observed a clear seasonal relationship between fraction sizes driven by water column stratification (freshwater input, nutrient availability, light availability, water temperature). Plankton abundance decreased proportionally with increasing size fraction, aligning with size spectra theory. Within the bay, greater mesozooplankton abundance, and a greater relative abundance of copepods, was observed closest to the aquaculture lease. No significant spatial effect was observed for phytoplankton composition. While the months of August to October showed statistically similar plankton composition and size spectra slopes (i.e., food chain efficiency) and could be used for interannual variability comparisons of plankton composition, sampling for longer periods could capture long-term phenological shifts in plankton abundance and composition related to various processes, including climate change. Conclusions provide guidance on optimal sampling to monitor and assess aquaculture pathways of effects.Cite this data as: Sharpe H, Lacoursière-Roussel A, Gallardi D (2024). Ecological insight of seasonal plankton succession to monitor shellfish aquaculture ecosystem interactions. Version 3.2. Fisheries and Oceans Canada. Sampling event dataset. https://doi.org/10.25607/2ujdvh

Plankton (zooplankton and large phytoplankton) are collected using the Continuous Plankton Recorder (CPR) in the Northwest (NW) Atlantic along tracks transited by container ships from Reykjavik (Iceland) to St. John’s, NL (the Z line), and between St. John’s and the New England Coast, along the Scotian Shelf (the E and MD lines). The CPR Survey is the longest running, most geographically extensive marine ecological survey in the world, providing comparable data on the geographical distribution, seasonal cycles and year-to-year changes in abundance of plankton over a large spatial area. The first northwest Atlantic samples were collected in the Irminger Sea in 1957, and sampling was extended farther west to the Scotian Shelf a few years later. Sampling has continued to the present with some interruptions during the late 1970s and 1980s. Sampling is nominally once per month along the E, MD, and Z lines. DFO Sample collection and analysis are led by the Continuous Plankton Recorder Survey program at the Marine Biological Association of the UK. DFO provides partial support for the northwest Atlantic survey carried out on the E, MD, and Z lines and incorporates CPR data in Atlantic Zone Monitoring Program ocean environmental status reporting.

The objective of this project was to gather data to develop a model of the food web of the lower trophic levels of the nearshore area of the Beaufort Sea. Sampling took place from 2005 to 2008 using the CCGS Nahidik. The multidisciplinary character of the Nahidik program produced measurements of biology/ecology (primary production, phytoplankton, zooplankton, benthos, fish), chemical and physical oceanography, contaminants, geology and hydro acoustics. The data were collected in July and August of each year. The Nahidik program provided data to provide a baseline for future studies as well as an information source for environmental assessment.This record contains water chemistry data collected as part of this project including suspended nitrogen, dissolved nitrogen, suspended phosphorus, dissolved phosphorus, dissolved organic carbon, suspended carbon, chlorophyll a, and suspended silicon.

From August 2nd to September 9th, 2013, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 64 stations along ten transects. Standardized sampling was conducted on the transects at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. A specialized CTD probe (UCTD) was deployed at an additional 72 locations while the ship was underway.Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

The objective of this project was to gather data to develop a model of the food web of the lower trophic levels of the nearshore area of the Beaufort Sea. Sampling took place from 2005 to 2008 using the CCGS Nahidik. The multidisciplinary character of the Nahidik program produced measurements of biology/ecology (primary production, phytoplankton, zooplankton, benthos, fish), chemical and physical oceanography, contaminants, geology and hydro acoustics. The data were collected in July and August of each year. The Nahidik program provided data to provide a baseline for future studies as well as an information source for environmental assessment. This record contains the geographic coordinates and station names from 2005 to 2008.

Bay-scale empirical demonstrations of how bivalve aquaculture alters plankton composition, and subsequently ecological functioning and higher trophic levels, are lacking. Temporal, inter- and within-bay variation in hydrodynamic, environmental, and aquaculture pressure limit efficient plankton monitoring design to detect bay-scale changes and inform aquaculture ecosystem interactions. Here, we used flow cytometry to investigate spatio-temporal variations in bacteria and phytoplankton (< 20 µm) composition in four bivalve aquaculture embayments. We observed higher abundances of bacteria and phytoplankton in shallow embayments that experienced greater freshwater and nutrient inputs. Depleted nutrient conditions may have led to the dominance of picophytoplankton cells, which showed strong within-bay variation as a function of riverine vs freshwater influence and nutrient availability. Although environmental forcings appeared to be a strong driver of spatio-temporal trends, results showed that bivalve aquaculture may reduce near-lease phytoplankton abundance and favor bacterial growth. We discuss aquaculture pathways of effects such as grazing, benthic-pelagic coupling processes, and microbial biogeochemical cycling. Conclusions provide guidance on optimal sampling considerations using flow cytometry in aquaculture sites based on embayment geomorphology and hydrodynamics.Cite this data as: Sharpe H, Lacoursière-Roussel A, Barrell J (2024). Monitoring bay-scale bivalve aquaculture ecosystem interactions using flow cytometry. Version 1.2. Fisheries and Oceans Canada. Samplingevent dataset. https://ipt.iobis.org/obiscanada/resource?r=monitoring_bay-scale_bivalve_aquaculture_ecosystem_interactions_using_flow_cytometry&v=1.2

This record contains information on the samples collected and the intended parameters for analysis from sediment cores collected as part of this project including bacteria, chemistry, stable isotopes, fatty acids, or meiofauna.

From August 5th to September 3rd, 2012, Fisheries and Oceans Canada conducted a baseline survey of marine fishes and their habitats on the Canadian Beaufort Shelf and slope. Sampling was conducted from the F/V Frosti at 28 stations along four transects. Standardized sampling was conducted at pre-determined depth stations (20-40, 75, 200, 350, 500, 750, and 1000 m) using a variety of sampling equipment including benthic fishing trawls, plankton nets, sediment cores, and CTD and water sample profiles. A specialized CTD probe (UCTD) was deployed at an additional 30 locations while the ship was underway. Presented here is the information on the sampling locations, and the sampling gear deployed at each station.

The North Shore of the Lower Estuary (Upper North Shore, Quebec) is a productive coastal system where many commercial species of benthic invertebrates are fished in the infralittoral (10-20 m) and circalittoral (20-50 m) zone. However, little data exist on the biodiversity of non-commercial species and the environmental characteristics of the benthic habitat in this area. Two scientific surveys were conducted in 2018 and 2019 to address this knowledge gap by developing a framework of biodiversity and environmental (water column and seafloor) data taking that will be used to determine the baseline state of the benthic ecosystem in this region.Surveys were conducted in 2018 (August 11-14) and 2019 (July 30-August 5) in the Upper North Shore region (between the towns of Forestville and Godbout). Surveys followed a fixed sampling design of eight transects perpendicular to bathymetry with stations at 10 m depth intervals in a bathymetry range of 10-50 m for a total of approximately 40 stations per survey. Specimens were collected with a beam trawl with an opening of 2.8 m. The hauls were made at a target speed of 2 knots and a target duration of 7 minutes. Start and end positions were recorded to calculate the distance traveled on each tow using the geosphere library of R. The average tow distance was approximately 425 m. The area covered at each tow was the product of the trawl opening and the distance traveled. The three files provided (DarwinCore format) are complementary and are linked by the "eventID" key. The "event_information" file includes generic event information, including date and location. The "additional_information_event_and_occurrence" file includes sample size, sampling protocol and sampling effort, among others. The "taxon_occurrence" file includes the taxonomy of the species observed, identified to the species or lowest possible taxonomic level. For abundance and biomass estimates, contact Virginie Roy (virginie.roy@dfo-mpo.gc.ca). For quality controls, all taxonomic names were checked against the World Register of Marine Species (WoRMS) to match recognized standards. The WoRMS match was placed in the "scientificNameID" field of the occurrence file. Special cases were noted in "identificationRemarks" and selected specimens were confirmed using field photos. Data quality checks were performed using the R obistools and worrms libraries. All sampling locations were spatially validated.This project was funded by DFO Coastal Environmental Baseline Program under Canada’s Oceans Protection Plan. This initiative aims to acquire environmental baseline data contributing to the characterization of important coastal areas and to support evidence-based assessments and management decisions for preserving marine ecosystems.

This project was completed by the Coastal Environmental Baseline Program (Coastal and Freshwater Ecology Section) in the Newfoundland and Labrador Science Branch of Fisheries and Oceans Canada (DFO). The nearshore fish and invertebrate community was sampled annually in Placentia Bay at 14 sites for 5 years (2018 – 2022). The objective of this program was to collect data on juvenile fish (specifically Atlantic cod) communities and habitats within Placentia Bay. The sites and timing (October) were selected based on historical sampling. There were several data types collected during each of these sampling trips. Boat-based beach seining was used to collect data on fish and invertebrate diversity, abundance, distribution, and size. Visual transects were used to identify vegetation and substrate (habitat) types and proportions at seine sites. CTD profiles (temperature, conductivity, depth, and salinity) were obtained using a castaway CTD. Finally, eDNA samples were collected at each site (2018-2021) to obtain complementary information on the presence of juvenile fish and other species present. This record contains the geographic locations of the 14 sites, and information on the timing and type of data collected at each site.