Marine Protected Areas require comprehensive monitoring to ensure objectives are achieved; however, monitoring natural ecosystems at scale is challenged by the biodiversity it aims to measure. Environmental DNA (eDNA) metabarcoding holds promise to address this monitoring challenge. We conducted paired sampling at 54 sites for fish and invertebrate assemblages in the Northwest Atlantic using groundfish trawls and eDNA metabarcoding of benthic seawater using four genetic markers (12S rRNA, 16S rRNA, 18S rRNA, and CO1). Compared to trawling, eDNA detected similar patterns of species turnover, larger estimates of gamma diversity, and smaller estimates of alpha diversity. A total of 63.6% (42/66) of fish species captured by trawling were detected by eDNA, along with an additional 26 species. Of the 24 missed detections by eDNA, 12 were inevitable as they lacked reference sequences. Excluding taxa assigned to higher than species level and those without a species name, 23.6% (17/72) of invertebrate species captured by trawling were detected by CO1, which detected an additional 98 species. We demonstrate that eDNA is capable of detecting patterns of community assemblage and species turnover in an offshore environment, emphasizing its strong potential for a non-invasive, comprehensive, and scalable tool for biodiversity monitoring supporting marine conservation programmes.Cite this data as: Jeffery, N., Rubidge, E., Abbott, C., Westfall, K., Stanley, R. (2024): Data of: eDNA metabarcoding enriches traditional trawl survey data for monitoring biodiversity in the marine environment.Published: August 2024. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, N.S. https://open.canada.ca/data/en/dataset/43a91ba7-8025-4330-88db-db14022d729d

This geodatabase includes hotspot maps of 1) nearshore habitat richness, 2) diversity (fish and invertebrates), and 3) biomass (using catch per unit effort of fish and invertebrates), as well as two layers showing the spatial extent of the diversity and biomass hotspot analyses. Full details and methods can be found in the Rubidge et al. 2018 CSAS Research Document 2018/053 available here or at https://waves-vagues.dfo-mpo.gc.ca/Library/40759842.pdf. These data were reviewed as part of a Canadian Science Advisory Secretariat (CSAS) regional peer review process on Nov 1-2, 2017.Habitat Richness Hotspots: Because there are no systematic surveys of nearshore species that span the entire coastline of Northern Shelf Bioregion, the nearshore habitat richness hotspots were developed as a proxy for species diversity in nearshore areas. Habitat richness was calculated from eight habitat features: eelgrass, surfgrass, canopy-forming kelp, estuaries, areas of high rugosity, and hard, mixed, and soft substrate. The number of features within 1 km x 1 km planning units was counted, and hotspots were identified using the Getis-Ord G* tool in ArcGIS. Planning units with Gi_Bin values of 3 (99% confidence) were classified as habitat richness hotspots.Diversity and Biomass Hotspots: Hotspots of fish and invertebrate diversity and biomass were developed as proxies for spatial patterns of productivity in the Northern Shelf Bioregion. Diversity (Shannon diversity) and biomass (kg/hour or count/hook/hour) were calculated from DFO synoptic trawl and outside hard-bottom longline (HBLL) survey catch records. The outside HBLL survey was previously referred to as Pacific Halibut Management Area (PHMA) survey. The synoptic trawl and HBLL surveys have complementary spatial coverage, with the HBLL surveys occurring in more coastal areas (20–260 m) and the synoptic trawl surveys occurring on deeper shelf areas (50–1300 m). Hotspots were identified using the Getis-Ord G* tool in ArcGIS for five separate analyses: fish biomass (trawl), fish diversity (trawl), fish diversity (longline), invertebrate biomass (trawl), and invertebrate diversity (trawl). Using the Minimum Bounding Geometry Tool, convex hull polygons were drawn around groups of hotspot points (Gi_Bin values of 1, 2, or 3; confidence ≥90%) containing 10 or more points. The resulting polygons were then buffered by 1 km and manually edited where needed to exclude any large areas of the polygons that did not include hotspot points.

Funded under DFO's Marine Conservation Targets Program in partnership with the Huntsman Marine Science Centre (HMSC), this diver-based imagery and sample collection benthic survey documents the occurrence of sponges at 42 dive sites in the Eastern Shore Islands (ESI) Area of Interest (AOI, ~2089 km2) off the Atlantic coast of Nova Scotia, Canada from dive surveys conducted in summer 2021 and 2022. Water quality, species occurrences and counts, habitat, slope, and substrate characteristics were catalogued through diver log sheets, camera imagery, specimen vouchers, and high-resolution bathymetric data. A total of 54 dives to depths from 11 to 33 m (below sea level), collecting up to 147 still images, one-hour of video, and 17 specimen samples per site, resulted in 220 observations for 27 different sponge taxa. This included three new records for Canada (Hymedesmia stellifera, Plocamionida arndti, Hymedesmia jecusculum) and a range extension for a species new to science (Crellomima mehqisinpekonuta) which was recently described from the Bay of Fundy. There were also four species which may seem to be new to science (Halichondria sp., Hymedesmia sp., Protosuberires sp., and Sphaerotylus sp.). Sponges were found to occupy a diversity of micro-habitats, often several different ones in proximity. A total of eight distinct habitat classes were defined, based on varying abundances and diversity of sponges and associated benthic species. These are likely widely distributed among the many complex submerged seabed features within this AOI. Collected specimens were preserved and are stored at the Atlantic Reference Centre (ARC) in St. Andrew's, New Brunswick.Cite this data as: Goodwin, C., Cooper, J.A., Lawton, P., Teed, L.L. 2025. Sponge occurrence and associated species and habitat descriptions derived from the 2021 and 2022 SCUBA diving surveys in the Eastern Shore Islands Area of Interest, Nova Scotia. Version 1.4. Fisheries and Oceans Canada. Occurrence dataset. https://ipt.iobis.org/obiscanada/resource?r=eastern_shore_islands_sponge_survey_2021_2022&v=1.4

DescriptionConservation of marine biodiversity requires understanding the joint influence of ongoing environmental change and fishing pressure. Addressing this challenge requires robust biodiversity monitoring and analyses that jointly account for potential drivers of change. Here, we ask how demersal fish biodiversity in Canadian Pacific waters has changed since 2003 and assess the degree to which these changes can be explained by environmental change and commercial fishing. Using a spatiotemporal multispecies model based on fisheries independent data, we find that species density (number of species per area) and community biomass have increased during this period. Environmental changes during this period were associated with temporal fluctuations in the biomass of species and the community as a whole. However, environmental changes were less associated with changes in species’ occurrence. Thus, the estimated increases in species density are not likely to be due to environmental change. Instead, our results are consistent with an ongoing recovery of the demersal fish community from a reduction in commercial fishing intensity from historical levels. These findings provide key insight into the drivers of biodiversity change that can inform ecosystem-based management.The layers provided represent three community metrics: 1) species density (i.e., species richness), 2) Hill-Shannon diversity, and 3) community biomass. All layers are provided at a 3 km resolution across the study domain for the period of 2003 to 2019. For each metric, we provide layers for three summary statistics: 1) the mean value in each grid cell over the temporal range, 2) the probability that the grid cell is a hotspot for that metric, and 3) the temporal coefficient of variation (i.e., standard deviation/mean) across all years.Methods:The analysis that produced these layers is presented in Thompson et al. (2022). The analysis uses data from the Groundfish Synoptic Bottom Trawl Research surveys in Queen Charlotte Sound (QCS), Hecate Strait (HS), West Coast Vancouver Island (WCVI), and West Coast Haida Gwaii (WCHG) from 2003 to 2019. Cartilaginous and bony fish species caught in DFO groundfish surveys that were present in at least 15% of all trawls over the depth range in which they were caught were included. This depth range was defined as that which included 95% of all trawls in which that species was present. The final dataset used in our analysis consisted of 57 species (Table S1 in Thompson et al. 2022).The spatiotemporal dynamics of the demersal fish community were modeled using the Hierarchical Modeling of Species Communities (HMSC) framework and package (Tikhonov et al. 2021) in R. This framework uses Bayesian inference to fit a multivariate hierarchical generalized mixed model. We modeled community dynamics using a hurdle model, which consists of two sub models: a presence-absence model and a biomass model that is conditional on presence. Our list of environmental covariates included bottom depth, bathymetric position index (BPI), mean summer tidal speed, substrate muddiness, substrate rockiness, whether the trawl was inside or outside of the ecosystem-based trawling footprint, and survey region (QCS & HS vs. WCVI & WCHG)), mean summer near-bottom temperature deviation, mean summer near-bottom dissolved oxygen deviation, mean summer cross-shore and along-shore current velocities near the seafloor, mean summer depth-integrated primary production, and local-scale commercial fishing effort.Layers are provided for three community metrics. All metrics should be interpreted as the value that would be expected in the catch from an average tow in the Groundfish Synoptic Bottom Trawl Research Surveys taken in a given 3 km grid cell. Species density (sometimes called species richness) should be interpreted as the number of the 57 species that would be caught in a trawl. Hill-Shannon diversity is a measure of diversity that gives greater weight to communities where biomass is spread equally across species. Community biomass is the total biomass across all 57 species that would be expected to be caught per square km in an average tow. Data Sources:Research data was provided by Pacific Science's Groundfish Data Unit for research surveys from the GFBio database between 2003 and 2019 that occurred in four regions: Queen Charlotte Sound, Hecate Strait, West Coast Haida Gwaii, and West Coast Vancouver Island. Our analysis excludes species that are rarely caught in the research trawls and so our estimates would not include the occurrence or biomass of these rare species.Commercial fishing data was accessed through a DFO R script detailed here: https://github.com/pbsassess/gfdata. Local scale commercial fishing effort was calculated from this data. The substrate layers were obtained from a substrate model (Gregr et al. 2021). The oceanographic layers (bottom temperature, dissolved oxygen, tidal and circulation speeds, primary production) were obtained from a hindcast simulation of the British Columbia continental margin (BCCM) model (Peña et al. 2019).Uncertainties:Species that are not well sampled by the trawl surveys may not be accurately estimated by our model. The model did not include spatiotemporal random effects, which likely underestimates spatiotemporal variability in the region. It is also important to underline covariate uncertainty and model uncertainty. The hotspot estimates provide one measure of model uncertainty/certainty.

Effective conservation planning relies on understanding population connectivity which can be informed by genomic data. This is particularly important for sessile species like the horse mussel (Modiolus modiolus), a key habitat-forming species and conservation priority in Atlantic Canada), yet little genomic information is available to describe horse mussel connectivity patterns. We used more than 8000 restriction-site associated DNA sequencing-derived single nucleotide polymorphisms and a panel of 8 microsatellites to examine genomic connectivity among horse mussel populations in the Bay of Fundy, along the Scotian Shelf, and in the broader northwestern Atlantic extending to Newfoundland. Despite phenotypic differences between sampling locations, we found an overall lack of genetic diversity and population structure in horse mussels in the Northwest Atlantic Ocean. All sampled locations had low heterozygosity, very low FST, elevated inbreeding coefficients, and deviated from Hardy-Weinberg Equilibrium, highlighting generally low genetic diversity across all metrics. Principal components analysis, Admixture analysis, pairwise FST calculations, and analysis of outlier loci (potentially under selection) all showed no independent genomic clusters within the data, and an analysis of molecular variance showed that less than 1% of the variation within the SNP dataset was found between sampling locations. Our results suggest that connectivity is high among horse mussel populations in the Northwest Atlantic, and coupled with large effective population sizes, this has resulted in minimal genomic divergence across the region. These results can inform conservation design considerations in the Bay of Fundy and support further integration into the broader regional conservation network.Cite this data as: Van Wyngaarden, Mallory et al. (2024). Widespread genetic similarity between Northwest Atlantic populations of the horse mussel, Modiolus modiolus. Published: May 2025. Coastal Ecosystem Science Division, Maritimes Region, Fisheries and Oceans Canada, Dartmouth, NS.

In celebration of the tremendous diversity of tree species that tell the story of our culture and history, the NCC released in September 2020 a compilation of close to 170 remarkable trees across Canada’s Capital region entitled A Living Legacy: Remarkable Trees of Canada’s Capital. An interactive map and downloadable book are available for free on the NCC’s website and will allow the public to discover distinctive features of these trees, revealing a story of the beauty of our natural heritage through the rich diversity of species thriving within Canada’s Capital. This compilation features trees according to their commonalities, which can include their physical relationship with the land, the fact that they were a source of food for Indigenous peoples, or for their contribution to the forest industry.https://ncc-ccn.gc.ca/remarkable-treeshttps://ncc-ccn.maps.arcgis.com/apps/MapJournal/index.html?appid=a9ba98fb7e8b4c2ba9be337235b95291

This dataset displays the Canadian geographic ranges of the priority species identified under the Pan-Canadian Approach for Transforming Species at Risk Conservation in Canada (“Pan-Canadian Approach”). These species include Barren-ground Caribou (including the Dolphin and Union population); Greater Sage-Grouse; Peary Caribou; Wood Bison; Caribou, Boreal population (“Boreal Caribou”); and Woodland Caribou, Southern Mountain population (“Southern Mountain Caribou”). The priority species were chosen following a number of criteria and considerations in collaboration with federal, provincial, and territorial partners. These include, but were not limited to, the species' ecological role on a regional or national scale, their conservation status and achievability of conservation outcomes, their social and cultural value (particularly to Indigenous peoples), and the leadership/partnership opportunities that they present. Delivering conservation outcomes for targeted priority species can have significant co-benefits for other species at risk, and wildlife in general. For more information on the Pan-Canadian Approach and the priority species, see https://www.canada.ca/en/services/environment/wildlife-plants-species/species-risk/pan-canadian-approach.html.This dataset includes: 1) the range for the Boreal Caribou (see https://species-registry.canada.ca/index-en.html#/consultations/2253); 2) the local populations for the Southern Mountain Caribou (see https://species-registry.canada.ca/index-en.html#/consultations/1309); 3) the range for the Greater Sage-Grouse (see https://species-registry.canada.ca/index-en.html#/consultations/1458); 4) local populations for the Peary Caribou (see https://species-registry.canada.ca/index-en.html#/consultations/3657); 5) range for the Barren-ground Caribou (see https://www.maps.geomatics.gov.nt.ca/Html5Viewer/index.html?viewer=NWT_SHV English only); 6) range for the Barren-ground Caribou, Dolphin and Union population (https://www.maps.geomatics.gov.nt.ca/Html5Viewer/index.html?viewer=NWT_SHV English only); 7) range for the Wood Bison (see https://species-registry.canada.ca/index-en.html#/consultations/2914).

This theme presents observations of invasive exotic species (IAS)transmitted and validated using the Sentinelle tool, an EEE detection system.An invasive exotic species is a plant, animal or microorganism (virus,bacteria or fungi) that are introduced outside of their natural range. Sonestablishment or its spread may pose a threat to the environment,the economy or society. The species listed are species of fauna and floraconcerning (or potentially worrying) for Quebec's biodiversity. Ellesinclude EEE present in Quebec and EEE not listed in Quebec atmonitor.**This third party metadata element was translated using an automated translation tool (Amazon Translate).**

The dataset consists of maps detailing the boundaries of the designatable units for conservation considerations as defined by the Committee on the Status of Endangered Wildlife in Canada (COSEWIC) for species of Pacific Salmon in British Columbia and the Yukon. Designatable units represent geographical areas that support groups of individuals with a unique genetic heritage makes them discrete and evolutionarily significant units of the taxonomic species, where “significant” means that the unit is important to the evolutionary legacy of the species as a whole and if lost is unlikely to be replaced through natural dispersion.

The Brier Island/Digby Neck area has been identified as an Ecologically and Biologically Significant Area (EBSA) by Fisheries and Oceans Canada and is one of four marine areas within the Bay of Fundy recognised by Parks Canada as of national significance for marine conservation planning. The area is representative of important outer Bay of Fundy features with significant marine mammal, bird, and benthic diversity including potentially important aggregations of sensitive benthic species such as horse mussel and sponge. Much of the information used for this recognition is now over 40 years old and should be re-validated using standardised georeferenced survey methods. As a first phase, a diver-based survey of the sublittoral habitats and associated species was conducted in August and September of 2017 for the Brier Island area. This report summarises the major sublittoral habitat types, species assemblages, and oceanographic conditions observed at 20 locations including Northwest and Southwest Ledges, Gull Rock, Peter’s Island, and Grand Passage. A total of 962 records were made of 178 taxa, consisting of 43 algae and 135 animals. Comparison with historical records largely confirmed the continued presence of unique habitats and species assemblages for which this area was initially recognised as an EBSA. Differences in species richness observed for cryptic and less known taxonomic groups such as sponges and bryozoans were attributable to changes in survey methods and knowledge. Based on these findings, additional surveys of inshore and offshore Brier Island using more quantitative methods developed for other Bay of Fundy EBSAs would further support regional MPA network planning and provide relative scales of species diversity and habitat coverage for this area.