A compendium of reports that provide information about aquatic and terrestrial animals and plants, soils, surface water, groundwater and their accompanying data files and maps

The Department of Fisheries and Oceans (DFO) Science Branch has designed a multispecies dive survey protocol to provide unbiased, coast wide monitoring of benthic invertebrate stocks (as may be required under the updated Fisheries Act) and associated habitat information for a suite of benthic marine invertebrate species (Green (Strongylocentrotus droebachiensis), Purple (S. purpuratus) and Red Sea Urchin (Mesocentrotus franciscanus), Geoduck (Panopea generosa), Giant Red Sea Cucumber (Apostichopus californicus), Northern Abalone (Haliotis kamtschatkana), and Sunflower Sea Star (Pycnopodia helianthoides)). Based on information available at this time, the proposed survey design can provide estimates of coast wide stock status for Red Sea Urchin and Giant Red Sea Cucumber, and relative abundance indices for Geoduck, Green Sea Urchin, Purple Sea Urchin, Northern Abalone and Sunflower Sea Star.The new protocol was tested through a series of pilot surveys conducted on a subset of areas of the BC coast each September from 2016-2021. Design of the pilot surveys was based on previous dive survey data and experience and demonstrated the practical feasibility of the protocol, while also gathering preliminary information to guide recommendations about the statistical design of the survey.The dataset consists of a relational database containing tables representing each component of the survey methodology. The primary component of the survey is a transect location. Along each transect, systematically spaced quadrats are sampled, and on each quadrat, substrate observations are recorded, multiple species of algae are recorded, and multiple individual invertebrates are measured or counted. The tables are linked by transect number and quadrat number.

Species characterization by environmental DNA (eDNA) is a method that allows the use of DNA released into the environment by organisms from various sources (secretions, faeces, gametes, tissues, etc.). It is a complementary tool to standard sampling methods for the identification of biodiversity. This project provides a list of fish and marine mammal species whose DNA has been detected in water samples collected between 2019 and 2021 using the mitochondrial marker MiFish (12S).The surveys were carried out in the summer of 2019 (July 14-18) and (July 30 - August 5), in the fall of 2020 (October 27-28) and in the summer-fall of 2021 (May 31 - June 3 ) and (August 24-25) between Forestville and Godbout (Haute-Côte-Nord). Sampling was carried out between 1-50 meters depth in 91 stations, with 1 to 3 replicates per station. Two liters of water were filtered through a 1.2 µm fiberglass filter. DNA extractions were performed with the DNeasy Blood and Tissues or PowerWater extraction kit (Qiagen). Negative field, extraction and PCR controls were added at the different stages of the protocol. The libraries were prepared either by Génome Québec (2019, 2020) or by the Genomics Laboratory of the Maurice-Lamontagne Institute (2021), then sequenced on a NovaSeq 4000 PE250 system by Génome Québec. The bioinformatics analysis of the sequences obtained was carried out using an analysis pipeline developed in the genomics laboratory. A first step made it possible to obtain a table of molecular operational taxonomic units (MOTU) using the cutadapt software for the removal of the adapters and the R package DADA2 for the filtration, the fusion, removal of chimeras and compilation of data. The MOTUs table was then corrected using the R package metabaR to eliminate the tag-jumping and take contaminants into consideration. Samples showing a strong presence of contaminating MOTUs were removed from the dataset. The MOTUs were also filtered to remove all remaining adapter sequences and also retain only those of the expected size (around 170 bp). Finally, taxonomic assignments were made on the MOTUs using the BLAST+ program and the NCBI-nt database. Taxonomic levels (species, genus or family) were assigned using a best match method (Top hit), with a threshold of 95%. Only assignments at the level of fish and marine mammals were considered, and the taxa detected were compared to a list of regional species, and corrected if necessary. The species detections of the different replicas have been combined.The file provided includes generic activity information, including site, station name, date, marker type, assignment types used for taxa identification, and a list of taxa or species. The list of taxa has been verified by a biodiversity expert from the Maurice-Lamontagne Institute.This project was funded by Fisheries and Oceans Canada's Coastal Environmental Baseline Data Program under the Oceans Protection Plan. This initiative aims to acquire baseline environmental data that contributes to the characterization of significant coastal areas and supports evidence-based assessments and management decisions to preserve marine ecosystems.Data were also published on SLGO platform : https://doi.org/10.26071/ogsl-2239bca5-c24a

The Great Lakes Fish Biodiversity Science Database is a compilation of fish community and habitat data from DFO Science surveys, primarily related to freshwater fishes of conservation concern in the Great Lakes basin. Data include: sampling site location, date, fish species and counts, and associated habitat information. Project-specific details including purpose/objectives and study methodology are often reported in the DFO Canadian data report of fisheries and aquatic sciences series.

Fish Habitat Assessment Output: 7 of 16High Water Level (75.4m ASL) - Juvenile/Adult Habitat - Low Vegetation Association Species (Coolwater Guild)Habitat suitability was assessed for the Bay of Quinte Area of Concern, at a 3 m grid resolution, using the Habitat Ecosystem Assessment Tool (HEAT), temperature algorithms, vegetation models, and water level input. Habitat classifications were based on three variables: depth (elevation), vegetation, and substrate; and modified by temperature suitabilities. The final suitability maps were based on documented habitat and temperature associations for the fish in the area. Different life stages (spawning requirements, nursery habitat, adult habitat) were modeled for the years of 1972-2011. Suitability values were scaled from 0 (not suitable) to 1 (highly suitable) and converted to suitability classes of very low, low, medium, and high. The final maps for each guild – life stage combination are maximum suitability values from the 39-year period modelled.

Fish Habitat Assessment Output: 6 of 16High Water Level (75.4m ASL) - Juvenile/Adult Habitat - Low Vegetation Association Species (Warmwater Guild)Habitat suitability was assessed for the Bay of Quinte Area of Concern, at a 3 m grid resolution, using the Habitat Ecosystem Assessment Tool (HEAT), temperature algorithms, vegetation models, and water level input. Habitat classifications were based on three variables: depth (elevation), vegetation, and substrate; and modified by temperature suitabilities. The final suitability maps were based on documented habitat and temperature associations for the fish in the area. Different life stages (spawning requirements, nursery habitat, adult habitat) were modeled for the years of 1972-2011. Suitability values were scaled from 0 (not suitable) to 1 (highly suitable) and converted to suitability classes of very low, low, medium, and high. The final maps for each guild – life stage combination are maximum suitability values from the 39-year period modelled.

Fish Habitat Assessment Output: 8 of 16High Water Level (75.4m ASL) - Juvenile/Adult Habitat - Low Vegetation Association Species (Coldwater) Habitat suitability was assessed for the Bay of Quinte Area of Concern, at a 3 m grid resolution, using the Habitat Ecosystem Assessment Tool (HEAT), temperature algorithms, vegetation models, and water level input. Habitat classifications were based on three variables: depth (elevation), vegetation, and substrate; and modified by temperature suitabilities. The final suitability maps were based on documented habitat and temperature associations for the fish in the area. Different life stages (spawning requirements, nursery habitat, adult habitat) were modeled for the years of 1972-2011. Suitability values were scaled from 0 (not suitable) to 1 (highly suitable) and converted to suitability classes of very low, low, medium, and high. The final maps for each guild – life stage combination are maximum suitability values from the 39-year period modelled.

Fish Habitat Assessment Output: 14 of 16Average Water Level (75.0m ASL) - Juvenile/Adult Habitat - Low Vegetation Association Species (Warmwater Guild)Habitat suitability was assessed for the Bay of Quinte Area of Concern, at a 3 m grid resolution, using the Habitat Ecosystem Assessment Tool (HEAT), temperature algorithms, vegetation models, and water level input. Habitat classifications were based on three variables: depth (elevation), vegetation, and substrate; and modified by temperature suitabilities. The final suitability maps were based on documented habitat and temperature associations for the fish in the area. Different life stages (spawning requirements, nursery habitat, adult habitat) were modeled for the years of 1972-2011. Suitability values were scaled from 0 (not suitable) to 1 (highly suitable) and converted to suitability classes of very low, low, medium, and high. The final maps for each guild – life stage combination are maximum suitability values from the 39-year period modelled.

The porbeagle shark (Lamna nasus), is a species found in Atlantic Canadian waters which is encountered in commercial and recreational fisheries. Pop-up Satellite Archival Tags (PSAT) from Wildlife Computers were applied to porbeagle sharks from 2005 to 2021 to collect data on depth (pressure), temperature and ambient light level (for position estimation). Deployments were conducted in Canada and the Faroe Islands on commercial, recreational and scientific charters, typically in summer and fall but some over winter when the porbeagle commercial fishery was active in Canada. A variety of tag models were deployed: PAT 4 (n=1), Mk10 (N=41), and MiniPAT (N=15) and 51 of 57 tags reported. One individual shark was recaptured and the physical tag was returned. The porbeagle sharks tagged ranged in size from 76 cm to 249 cm Fork Length (curved); 42 were female, 15 were male. Time at liberty ranged from 4 – 356 days and 14 tags remained on for the programmed duration. Raw data transmitted from the PSAT’s after release was processed through Wildlife Computers software (GPE3) to get summary files, assuming a maximum swimming speed of 2m/s, NOAA OI SST V2 High Resolution data set for SST reference and ETOPO1-Bedrock dataset for bathymetry reference. The maximum likelihood position estimates are available in .csv and .kmz format and depth and temperature profiles are also in .csv format. Other tag outputs as well as metadata from the deployments can be obtained upon request from: warren.joyce@dfo-mpo.gc.ca or heather.bowlby@dfo-mpo.gc.ca.

Fish Habitat Assessment Output: 5 of 16High Water Level (75.4m ASL) - Juvenile/Adult Habitat - High Vegetation Association Species (All Thermal Guilds)Habitat suitability was assessed for the Bay of Quinte Area of Concern, at a 3 m grid resolution, using the Habitat Ecosystem Assessment Tool (HEAT), temperature algorithms, vegetation models, and water level input. Habitat classifications were based on three variables: depth (elevation), vegetation, and substrate; and modified by temperature suitabilities. The final suitability maps were based on documented habitat and temperature associations for the fish in the area. Different life stages (spawning requirements, nursery habitat, adult habitat) were modeled for the years of 1972-2011. Suitability values were scaled from 0 (not suitable) to 1 (highly suitable) and converted to suitability classes of very low, low, medium, and high. The final maps for each guild – life stage combination are maximum suitability values from the 39-year period modelled.